{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T23:32:05Z","timestamp":1792711925381,"version":"4.5.0"},"reference-count":28,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2012,10,7]],"date-time":"2012-10-07T00:00:00Z","timestamp":1349568000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2012,10,7]],"date-time":"2012-10-07T00:00:00Z","timestamp":1349568000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Arch Virol"],"published-print":{"date-parts":[[2013,2]]},"DOI":"10.1007\/s00705-012-1489-2","type":"journal-article","created":{"date-parts":[[2012,10,6]],"date-time":"2012-10-06T05:36:10Z","timestamp":1349501770000},"page":"349-358","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":75,"title":["Griffithsin inhibits Japanese encephalitis virus infection in vitro and in vivo"],"prefix":"10.1007","volume":"158","author":[{"given":"Hassan Z. A.","family":"Ishag","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming-xia","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengjuan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Ni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thowaiba","family":"Malik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pu-yan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Mao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2012,10,7]]},"reference":[{"key":"1489_CR1","doi-asserted-by":"publisher","first-page":"875","DOI":"10.1016\/j.chembiol.2004.04.010","volume":"11","author":"EW Adams","year":"2004","unstructured":"Adams EW, Ratner DM, Bokesch HR, McMahon JB, O\u2019Keefe BR, Seeberger PH (2004) Oligosaccharide and glycoprotein microarrays as tools in HIV glycobiology: glycan-dependent gp120\/protein interactions. Chem Biol 11:875\u2013881","journal-title":"Chem Biol"},{"key":"1489_CR2","doi-asserted-by":"publisher","first-page":"9039","DOI":"10.1128\/JVI.02675-10","volume":"85","author":"KB Alexandre","year":"2011","unstructured":"Alexandre KB, Gray ES, Pantophlet R, Moore PL, McMahon JB, Chakauya E, O\u2019Keefe BR, Chikwamba R, Morris L (2011) Binding of the mannose-specific lectin, griffithsin, to HIV-1 gp120 exposes the CD4-binding site. J Virol 85:9039","journal-title":"J Virol"},{"key":"1489_CR3","doi-asserted-by":"publisher","first-page":"583","DOI":"10.1038\/nrmicro1707","volume":"5","author":"J Balzarini","year":"2007","unstructured":"Balzarini J (2007) Targeting the glycans of glycoproteins: a novel paradigm for antiviral therapy. Nat Rev Microbiol 5:583\u2013597","journal-title":"Nat Rev Microbiol"},{"key":"1489_CR4","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1016\/S0026-895X(24)12550-3","volume":"59","author":"AJ Bolmstedt","year":"2001","unstructured":"Bolmstedt AJ, O\u2019Keefe BR, Shenoy SR, McMahon JB, Boyd MR (2001) Cyanovirin-N defines a new class of antiviral agent targeting N-linked, high-mannose glycans in an oligosaccharide-specific manner. Mol Pharmacol 59:949\u2013954","journal-title":"Mol Pharmacol"},{"key":"1489_CR5","doi-asserted-by":"publisher","first-page":"577","DOI":"10.1093\/infdis\/162.3.577","volume":"162","author":"WE Brandt","year":"1990","unstructured":"Brandt WE (1990) From the World Health Organization: development of dengue and Japanese encephalitis vaccines. J Infect Dis 162:577\u2013583","journal-title":"J Infect Dis"},{"key":"1489_CR6","doi-asserted-by":"publisher","first-page":"728","DOI":"10.1038\/sj.emboj.7600064","volume":"23","author":"S Bressanelli","year":"2004","unstructured":"Bressanelli S, Stiasny K, Allison SL, Stura EA, Duquerroy S, Lescar J, Heinz FX, Rey FA (2004) Structure of a flavivirus envelope glycoprotein in its low-pH-induced membrane fusion conformation. EMBO J 23:728\u2013738","journal-title":"EMBO J"},{"key":"1489_CR7","doi-asserted-by":"publisher","first-page":"579","DOI":"10.1007\/s00253-007-0978-9","volume":"76","author":"Y Cao","year":"2007","unstructured":"Cao Y, Qiao J, Li Y, Lu W (2007) De novo synthesis, constitutive expression of Aspergillus sulphureus \u03b2-xylanase gene in Pichia pastoris and partial enzymic characterization. Appl Microbiol Biotechnol 76:579\u2013585","journal-title":"Appl Microbiol Biotechnol"},{"key":"1489_CR8","first-page":"1","volume":"12","author":"YH Chiu","year":"2011","unstructured":"Chiu YH, Chan Y-L, Li T-L, Wu CJ (2011) Inhibition of Japanese encephalitis virus infection by the sulfated polysaccharides extracts from Ulva lactuca. Mar Biotechnol 12:1\u201311","journal-title":"Mar Biotechnol"},{"key":"1489_CR9","first-page":"326","volume":"98","author":"K Dutta","year":"2010","unstructured":"Dutta K, Rangarajan PN, Vrati S, Basu A (2010) Japanese encephalitis: pathogenesis, prophylactics and therapeutics. Curr Sci 98:326","journal-title":"Curr Sci"},{"key":"1489_CR10","doi-asserted-by":"publisher","first-page":"244","DOI":"10.1111\/j.1600-0684.2007.00242.x","volume":"36","author":"P Emau","year":"2007","unstructured":"Emau P, Tian B, O\u2019keefe B, Mori T, McMahon J, Palmer K, Jiang Y, Bekele G, Tsai C (2007) Griffithsin, a potent HIV entry inhibitor, is an excellent candidate for anti-HIV microbicide. J Med Primatol 36:244\u2013253","journal-title":"J Med Primatol"},{"key":"1489_CR11","doi-asserted-by":"publisher","first-page":"194","DOI":"10.1016\/j.pep.2005.10.014","volume":"47","author":"B Giomarelli","year":"2006","unstructured":"Giomarelli B, Schumacher KM, Taylor TE, Sowder RC 2nd, Hartley JL, McMahon JB, Mori T (2006) Recombinant production of anti-HIV protein, griffithsin, by auto-induction in a fermentor culture. Protein Expres Purif 47:194\u2013202","journal-title":"Protein Expres Purif"},{"key":"1489_CR12","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1016\/S0065-3527(08)60433-9","volume":"27","author":"C Huang","year":"1982","unstructured":"Huang C (1982) Studies of Japanese encephalitis in China. Adv Virus Res 27:71\u2013101","journal-title":"Adv Virus Res"},{"key":"1489_CR13","doi-asserted-by":"publisher","first-page":"7846","DOI":"10.1128\/JVI.00789-08","volume":"82","author":"JM Kim","year":"2008","unstructured":"Kim JM, Yun SI, Song BH, Hahn YS, Lee CH, Oh HW, Lee YM (2008) A single N-linked glycosylation site in the Japanese encephalitis virus prM protein is critical for cell type-specific prM protein biogenesis, virus particle release, and pathogenicity in mice. J Virol 82:7846","journal-title":"J Virol"},{"key":"1489_CR14","first-page":"1108","volume-title":"Fields virology","author":"BD Lindenbach","year":"2007","unstructured":"Lindenbach BD, Thiel H-Ju, Rice CM (2007) Flaviviridae: the viruses and their replication. In: Knipe DM, Howley PM (eds) Fields virology. Lippincott-Raven, Philadelphia, pp 1108\u20131109"},{"key":"1489_CR15","doi-asserted-by":"publisher","first-page":"5159","DOI":"10.1128\/AAC.00633-11","volume":"55","author":"P Meuleman","year":"2011","unstructured":"Meuleman P, Albecka A, Belouzard S, Vercauteren K, Verhoye L, Wychowski C, Leroux-Roels G, Palmer KE, Dubuisson J (2011) Griffithsin has antiviral activity against hepatitis C virus. Antimicrob Agents Ch 55:5159\u20135167","journal-title":"Antimicrob Agents Ch"},{"key":"1489_CR16","doi-asserted-by":"publisher","first-page":"9345","DOI":"10.1074\/jbc.M411122200","volume":"280","author":"T Mori","year":"2005","unstructured":"Mori T, O\u2019Keefe BR, Sowder RC 2nd, Bringans S, Gardella R, Berg S, Cochran P, Turpin JA, Buckheit RW Jr, McMahon JB, Boyd MR (2005) Isolation and characterization of griffithsin, a novel HIV-inactivating protein, from the red alga Griffithsia sp. J Biol Chem 280:9345\u20139353","journal-title":"J Biol Chem"},{"key":"1489_CR17","doi-asserted-by":"publisher","first-page":"6099","DOI":"10.1073\/pnas.0901506106","volume":"106","author":"BR O\u2019Keefe","year":"2009","unstructured":"O\u2019Keefe BR, Vojdani F, Buffa V, Shattock RJ, Montefiori DC, Bakke J, Mirsalis J, d\u2019Andrea AL, Hume SD, Bratcher B (2009) Scaleable manufacture of HIV-1 entry inhibitor griffithsin and validation of its safety and efficacy as a topical microbicide component. PNAS 106:6099","journal-title":"PNAS"},{"key":"1489_CR18","doi-asserted-by":"publisher","first-page":"2511","DOI":"10.1128\/JVI.02322-09","volume":"84","author":"BR O\u2019Keefe","year":"2010","unstructured":"O\u2019Keefe BR, Giomarelli B, Barnard DL, Shenoy SR, Chan PKS, McMahon JB, Palmer KE, Barnett BW, Meyerholz DK, Wohlford-Lenane CL (2010) Broad-spectrum in vitro activity and in vivo efficacy of the antiviral protein griffithsin against emerging viruses of the family Coronaviridae. J Virol 84:2511","journal-title":"J Virol"},{"key":"1489_CR19","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1093\/oxfordjournals.aje.a118408","volume":"27","author":"LJ Reed","year":"1938","unstructured":"Reed LJ, Muench H (1938) A simple method of estimating fifty per cent endpoints. Am J Epidemiol 27:493\u2013497","journal-title":"Am J Epidemiol"},{"key":"1489_CR20","doi-asserted-by":"publisher","first-page":"194","DOI":"10.1006\/abio.2000.4753","volume":"285","author":"TD Schmittgen","year":"2000","unstructured":"Schmittgen TD, Zakrajsek BA, Mills AG, Gorn V, Singer MJ, Reed MW (2000) Quantitative reverse transcription-polymerase chain reaction to study mRNA decay: comparison of endpoint and real-time methods. Anal Biochem 285:194\u2013204","journal-title":"Anal Biochem"},{"key":"1489_CR21","doi-asserted-by":"publisher","first-page":"64","DOI":"10.1186\/1743-422X-5-64","volume":"5","author":"L Sebastian","year":"2008","unstructured":"Sebastian L, Desai A, Shampur MN, Perumal Y, Sriram D, Vasanthapuram R (2008) N-methylisatin-beta-thiosemicarbazone derivative (SCH 16) is an inhibitor of Japanese encephalitis virus infection in vitro and in vivo. Virol J 5:64","journal-title":"Virol J"},{"key":"1489_CR22","doi-asserted-by":"publisher","first-page":"168","DOI":"10.1016\/j.ijantimicag.2008.07.013","volume":"33","author":"L Sebastian","year":"2009","unstructured":"Sebastian L, Desai A, Madhusudana SN, Ravi V (2009) Pentoxifylline inhibits replication of Japanese encephalitis virus: a comparative study with ribavirin. Int J Antimicrob Agents 33:168\u2013173","journal-title":"Int J Antimicrob Agents"},{"key":"1489_CR23","doi-asserted-by":"publisher","first-page":"497","DOI":"10.1016\/0042-6822(87)90144-9","volume":"161","author":"H Sumiyoshi","year":"1987","unstructured":"Sumiyoshi H, Mori C, Fuke I, Morita K, Kuhara S, Kondou J, Kikuchi Y, Nagamatu H, Igarashi A (1987) Complete nucleotide sequence of the Japanese encephalitis virus genome RNA. Virology 161:497\u2013510","journal-title":"Virology"},{"key":"1489_CR24","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1006\/abio.1999.4085","volume":"270","author":"J Winer","year":"1999","unstructured":"Winer J, Jung CKS, Shackel I, Williams PM (1999) Development and validation of Real-Time quantitative reverse transcriptase-polymerase chain reaction for monitoring gene expression in cardiac myocytesin vitro. Anal Biochem 270:41\u201349","journal-title":"Anal Biochem"},{"key":"1489_CR25","doi-asserted-by":"publisher","first-page":"361","DOI":"10.7150\/ijbs.6.361","volume":"6","author":"Y Yan","year":"2010","unstructured":"Yan Y, Kang B (2010) Regulation of Vid-dependent degradation of FBPase by TCO89, a component of TOR Complex 1. Int J Biol Sci 6:361","journal-title":"Int J Biol Sci"},{"key":"1489_CR26","doi-asserted-by":"publisher","first-page":"80","DOI":"10.1186\/1743-422X-8-80","volume":"8","author":"Y Yang","year":"2011","unstructured":"Yang Y, Ye J, Yang X, Jiang R, Chen H, Cao S (2011) Japanese encephalitis virus infection induces changes of mRNA profile of mouse spleen and brain. Virol J 8:80","journal-title":"Virol J"},{"key":"1489_CR27","doi-asserted-by":"publisher","first-page":"1127","DOI":"10.1016\/j.str.2006.05.017","volume":"14","author":"NE Ziolkowska","year":"2006","unstructured":"Ziolkowska NE, O\u2019Keefe BR, Mori T, Zhu C, Giomarelli B, Vojdani F, Palmer KE, McMahon JB, Wlodawer A (2006) Domain-swapped structure of the potent antiviral protein griffithsin and its mode of carbohydrate binding. Structure\u00a014:1127\u20131135","journal-title":"Structure"},{"key":"1489_CR28","doi-asserted-by":"crossref","first-page":"617","DOI":"10.18388\/abp.2006_3290","volume":"53","author":"NE Zi\u00f3\u0142kowska","year":"2006","unstructured":"Zi\u00f3\u0142kowska NE, Wlodawer A (2006) Structural studies of algal lectins with anti-HIV activity. Acta Biochim Pol 53:617\u2013626","journal-title":"Acta Biochim Pol"}],"container-title":["Archives of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00705-012-1489-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00705-012-1489-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00705-012-1489-2","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00705-012-1489-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T04:12:40Z","timestamp":1744171960000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00705-012-1489-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,10,7]]},"references-count":28,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2013,2]]}},"alternative-id":["1489"],"URL":"https:\/\/doi.org\/10.1007\/s00705-012-1489-2","relation":{},"ISSN":["0304-8608","1432-8798"],"issn-type":[{"value":"0304-8608","type":"print"},{"value":"1432-8798","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,10,7]]},"assertion":[{"value":"8 June 2012","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 August 2012","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 October 2012","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}]}}