{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T15:26:36Z","timestamp":1786980396643,"version":"build-2736575974"},"reference-count":26,"publisher":"Springer Science and Business Media LLC","issue":"9","license":[{"start":{"date-parts":[[2016,6,10]],"date-time":"2016-06-10T00:00:00Z","timestamp":1465516800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2016,6,10]],"date-time":"2016-06-10T00:00:00Z","timestamp":1465516800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100012421","name":"Agricultural\u00a0Science\u00a0and\u00a0Technology\u00a0Innovation\u00a0Program","doi-asserted-by":"crossref","award":["ASTIP-IAS15"],"award-info":[{"award-number":["ASTIP-IAS15"]}],"id":[{"id":"10.13039\/501100012421","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Arch Virol"],"published-print":{"date-parts":[[2016,9]]},"DOI":"10.1007\/s00705-016-2918-4","type":"journal-article","created":{"date-parts":[[2016,6,10]],"date-time":"2016-06-10T02:28:54Z","timestamp":1465525734000},"page":"2543-2547","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Establishment of a nanoparticle-assisted RT-PCR assay to distinguish field strains and attenuated strains of porcine epidemic diarrhea virus"],"prefix":"10.1007","volume":"161","author":[{"given":"Yu","family":"Zhu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gui-hua","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-dong","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shang-jin","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,6,10]]},"reference":[{"key":"2918_CR1","first-page":"594","volume":"135","author":"K Van Reeth","year":"1994","unstructured":"Van Reeth K, Pensaert M (1994) Prevalence of infections with enzootic respiratory and enteric viruses in feeder pigs entering fattening herds. Vet Rec 135:594\u2013597","journal-title":"Vet Rec"},{"key":"2918_CR2","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1136\/vr.100.12.243","volume":"100","author":"EN Wood","year":"1977","unstructured":"Wood EN (1977) An apparently new syndrome of porcine epidemic diarrhoea. Vet Rec 100:243\u2013244","journal-title":"Vet Rec"},{"key":"2918_CR3","doi-asserted-by":"publisher","first-page":"1112","DOI":"10.3201\/eid1507.081256","volume":"15","author":"S Puranaveja","year":"2009","unstructured":"Puranaveja S, Poolperm P, Lertwatcharasarakul P, Kesdaengsakonwut S, Boonsoongnern A, Urairong K, Kitikoon P, Choojai P, Kedkovid R, Teankum K, Thanawongnuwech R (2009) Chinese-like strain of porcine epidemic diarrheavirus, Thailand. Emerg Infect Dis 15:1112\u20131115","journal-title":"Emerg Infect Dis"},{"key":"2918_CR4","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1016\/S0021-9975(05)80069-6","volume":"113","author":"M Sueyoshi","year":"1995","unstructured":"Sueyoshi M, Tsuda T, Yamazaki K, Yoshida K, Nakazawa M, Sato K, Minami T, Iwashita K, Watanabe M, Suzuki Y (1995) An immunohistochemical investigation of porcine epidemic diarrhoea. J Comp Pathol 113:59\u201367","journal-title":"J Comp Pathol"},{"key":"2918_CR5","doi-asserted-by":"publisher","first-page":"161","DOI":"10.3201\/eid1801.111259","volume":"18","author":"RQ Sun","year":"2012","unstructured":"Sun RQ, Cai RJ, Chen YQ, Liang PS, Chen DK, Song CX (2012) Outbreak of porcine epidemic diarrhea in suckling piglets, China. Emerg Infect Dis 18:161\u2013163","journal-title":"Emerg Infect Dis"},{"key":"2918_CR6","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1007\/s11262-012-0713-1","volume":"44","author":"D Song","year":"2012","unstructured":"Song D, Park B (2012) Porcine epidemic diarrhoea virus: a comprehensive review of molecular epidemiology, diagnosis, and vaccines. Virus Genes 44:167\u2013175","journal-title":"Virus Genes"},{"key":"2918_CR7","first-page":"467","volume":"243","author":"G Cima","year":"2013","unstructured":"Cima G (2013) Fighting a deadly pig disease. Industry, veterinarians trying to contain PED virus, new to the US. J Am Vet Med Assoc 243:467\u2013470","journal-title":"J Am Vet Med Assoc"},{"key":"2918_CR8","doi-asserted-by":"publisher","first-page":"234","DOI":"10.1128\/JCM.02820-13","volume":"52","author":"Q Chen","year":"2014","unstructured":"Chen Q, Li G, Stasko J, Thomas JT, Stensland WR, Pillatzki AE, Gauger PC, Schwartz KJ, Madson D, Yoon KJ, Stevenson GW, Burrough ER, Harmon KM, Main RG, Zhang J (2014) Isolation and characterization of porcine epidemic diarrhea viruses associated with the 2013 disease outbreak among swine in the US. J Clin Microbiol 52:234\u2013243","journal-title":"J Clin Microbiol"},{"key":"2918_CR9","doi-asserted-by":"publisher","first-page":"6121","DOI":"10.1128\/JVI.00430-15","volume":"89","author":"C Liu","year":"2015","unstructured":"Liu C, Tang J, Ma Y, Liang X, Yang Y, Peng G, Qi Q, Jiang S, Li J, Du L, Li F (2015) Receptor usage and cell entry of porcine epidemic diarrhea coronavirus. J Virol 89:6121\u20136125","journal-title":"J Virol"},{"key":"2918_CR10","doi-asserted-by":"publisher","first-page":"202","DOI":"10.1016\/j.jbiotec.2010.07.019","volume":"150","author":"X Ren","year":"2010","unstructured":"Ren X, Li G, Liu B (2010) Binding characterization of determinants in porcine aminopeptidase N, the cellular receptor for transmissible gastroenteritis virus. J Biotechnol 150:202\u2013206","journal-title":"J Biotechnol"},{"key":"2918_CR11","doi-asserted-by":"publisher","first-page":"12540","DOI":"10.1073\/pnas.0605402103","volume":"103","author":"W Lu","year":"2006","unstructured":"Lu W, Zheng BJ, Xu K, Schwarz W, Du L, Wong CK, Chen J, Duan S, Deubel V, Sun B (2006) Severe acute respiratory syndrome-associated coronavirus 3a protein forms an ion channel and modulates virus release. Proc Natl Acad Sci USA 103:12540\u201312545","journal-title":"Proc Natl Acad Sci USA"},{"key":"2918_CR12","doi-asserted-by":"publisher","first-page":"763","DOI":"10.1007\/s007050050541","volume":"144","author":"A McGoldrick","year":"1999","unstructured":"McGoldrick A, Lowings JP, Paton DJ (1999) Characterisation of a recent virulent transmissible gastroenteritis virus from Britain with a deleted ORF 3a. Arch Virol 144:763\u2013770","journal-title":"Arch Virol"},{"key":"2918_CR13","doi-asserted-by":"publisher","first-page":"1833","DOI":"10.1016\/S0264-410X(03)00027-6","volume":"21","author":"DS Song","year":"2003","unstructured":"Song DS, Yang JS, Oh JS, Han JH, Park BK (2003) Differentiation of a Vero cell adapted porcine epidemic diarrhea virus from Korean field strains by restriction fragment length polymorphism analysis of ORF 3. Vaccine 21:1833\u20131842","journal-title":"Vaccine"},{"key":"2918_CR14","doi-asserted-by":"publisher","first-page":"384","DOI":"10.1016\/j.febslet.2012.01.005","volume":"586","author":"K Wang","year":"2012","unstructured":"Wang K, Lu W, Chen J, Xie S, Shi H, Hsu H, Yu W, Xu K, Bian C, Fischer WB, Schwarz W, Feng L, Sun B (2012) PEDV ORF3 encodes an ion channel protein and regulates virus production. FEBS Lett 586:384\u2013391","journal-title":"FEBS Lett"},{"key":"2918_CR15","doi-asserted-by":"publisher","first-page":"577","DOI":"10.1007\/s00705-010-0892-9","volume":"156","author":"SJ Park","year":"2011","unstructured":"Park SJ, Kim HK, Song DS, Moon HJ, Park BK (2011) Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus (PEDV) field isolates in Korea. Arch Virol 156:577\u2013585","journal-title":"Arch Virol"},{"key":"2918_CR16","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1292\/jvms.54.313","volume":"54","author":"K Kusanagi","year":"1992","unstructured":"Kusanagi K, Kuwahara H, Katoh T, Nunoya T, Ishikawa Y, Samejima T, Tajima M (1992) Isolation and serial propagation of porcine epidemic diarrhea virus in cell cultures and partial characterization of the isolate. J Vet Med Sci 54:313\u2013318","journal-title":"J Vet Med Sci"},{"key":"2918_CR17","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1016\/j.mcp.2014.11.004","volume":"29","author":"H Gou","year":"2015","unstructured":"Gou H, Deng J, Wang J, Pei J, Liu W, Zhao M, Chen J (2015) Rapid and sensitive detection of porcine epidemic diarrhea virus by reverse transcription loop-mediated isothermal amplification combined with a vertical flow visualization strip. Mol Cell Probes 29:48\u201353","journal-title":"Mol Cell Probes"},{"key":"2918_CR18","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1177\/1040638715621949","volume":"28","author":"LC Miller","year":"2016","unstructured":"Miller LC, Crawford KK, Lager KM, Kellner SG, Brockmeier SL (2016) Evaluation of two real-time polymerase chain reaction assays for Porcine epidemic diarrhea virus (PEDV) to assess PEDV transmission in growing pigs. J Vet Diagn Invest 28:20\u201329","journal-title":"J Vet Diagn Invest"},{"key":"2918_CR19","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1177\/104063870401600312","volume":"16","author":"K Jung","year":"2004","unstructured":"Jung K, Chae C (2004) Effect of temperature on the detection of porcine epidemic diarrhea virus and transmissible gastroenteritis virus in fecal samples by reverse transcription-polymerase chain reaction. J Vet Diagn Invest 16:237\u2013239","journal-title":"J Vet Diagn Invest"},{"key":"2918_CR20","doi-asserted-by":"publisher","first-page":"455103","DOI":"10.1088\/0957-4484\/20\/45\/455103","volume":"20","author":"C Shen","year":"2009","unstructured":"Shen C, Yang W, Ji Q, Maki H, Dong A, Zhang Z (2009) NanoPCR observation: different levels of DNA replication fidelity in nanoparticle-enhanced polymerase chain reactions. Nanotechnology 20:455103","journal-title":"Nanotechnology"},{"key":"2918_CR21","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1016\/j.tvjl.2015.02.017","volume":"204","author":"K Jung","year":"2015","unstructured":"Jung K, Saif LJ (2015) Porcine epidemic diarrhea virus infection: Etiology, epidemiology, pathogenesis and immunoprophylaxis. Vet J 204:134\u2013143","journal-title":"Vet J"},{"key":"2918_CR22","doi-asserted-by":"publisher","first-page":"1471","DOI":"10.1007\/s00705-010-0720-2","volume":"155","author":"J Chen","year":"2010","unstructured":"Chen J, Wang C, Shi H, Qiu H, Liu S, Chen X, Zhang Z, Feng L (2010) Molecular epidemiology of porcine epidemic diarrhea virus in China. Arch Virol 155:1471\u20131476","journal-title":"Arch Virol"},{"key":"2918_CR23","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1007\/s11262-012-0735-8","volume":"45","author":"ZL Li","year":"2012","unstructured":"Li ZL, Zhu L, Ma JY, Zhou QF, Song YH, Sun BL, Chen RA, Xie QM, Bee YZ (2012) Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus (PEDV) field strains in south China. Virus Genes 45:181\u2013185","journal-title":"Virus Genes"},{"key":"2918_CR24","doi-asserted-by":"publisher","first-page":"374","DOI":"10.1016\/j.jviromet.2013.07.018","volume":"193","author":"X Ma","year":"2013","unstructured":"Ma X, Cui Y, Qiu Z, Zhang B, Cui S (2013) A nanoparticle-assisted PCR assay to improve the sensitivity for rapid detection and differentiation of wild-type pseudorabies virus and gene-deleted vaccine strains. J Virol Methods 193:374\u2013378","journal-title":"J Virol Methods"},{"key":"2918_CR25","doi-asserted-by":"publisher","first-page":"106","DOI":"10.1016\/j.jviromet.2014.02.029","volume":"202","author":"X Wang","year":"2014","unstructured":"Wang X, Bai A, Zhang J, Kong M, Cui Y, Ma X, Ai X, Tang Q, Cui S (2014) A new nanoPCR molecular assay for detection of porcine bocavirus. J Virol Methods 202:106\u2013111","journal-title":"J Virol Methods"},{"key":"2918_CR26","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1016\/j.jviromet.2015.04.008","volume":"220","author":"W Yuan","year":"2015","unstructured":"Yuan W, Li Y, Li P, Song Q, Li L, Sun J (2015) Development of a nanoparticle-assisted PCR assay for detection of porcine epidemic diarrhea virus. J Virol Methods 220:18\u201320","journal-title":"J Virol Methods"}],"container-title":["Archives of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00705-016-2918-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00705-016-2918-4\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00705-016-2918-4","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00705-016-2918-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T01:04:50Z","timestamp":1744160690000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00705-016-2918-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,6,10]]},"references-count":26,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2016,9]]}},"alternative-id":["2918"],"URL":"https:\/\/doi.org\/10.1007\/s00705-016-2918-4","relation":{},"ISSN":["0304-8608","1432-8798"],"issn-type":[{"value":"0304-8608","type":"print"},{"value":"1432-8798","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,6,10]]},"assertion":[{"value":"17 March 2016","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"31 May 2016","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 June 2016","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"None of the authors of this paper has a financial or personal relationship with other people or organizations that could inappropriately influence or bias the content of the paper.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}