{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T17:00:43Z","timestamp":1779296443692,"version":"3.51.4"},"reference-count":33,"publisher":"Springer Science and Business Media LLC","issue":"8","license":[{"start":{"date-parts":[[2017,4,18]],"date-time":"2017-04-18T00:00:00Z","timestamp":1492473600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Guangdong Provincial Science and Technology","award":["2010A040302003"],"award-info":[{"award-number":["2010A040302003"]}]},{"name":"Guangdong Provincial Science and Technology","award":["2011B031800163"],"award-info":[{"award-number":["2011B031800163"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["NO.31670168"],"award-info":[{"award-number":["NO.31670168"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"The 12th five-year-major-projects of China\u2019s Ministry of Public Health","award":["2012zx10004-213"],"award-info":[{"award-number":["2012zx10004-213"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Arch Virol"],"published-print":{"date-parts":[[2017,8]]},"DOI":"10.1007\/s00705-017-3361-x","type":"journal-article","created":{"date-parts":[[2017,4,18]],"date-time":"2017-04-18T07:51:33Z","timestamp":1492501893000},"page":"2445-2449","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Two deletion variants of Middle East respiratory syndrome coronavirus found in a patient with characteristic symptoms"],"prefix":"10.1007","volume":"162","author":[{"given":"Qian","family":"Xie","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yujuan","family":"Cao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Su","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianbo","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengsong","family":"Wan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingliang","family":"He","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changwen","family":"Ke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2017,4,18]]},"reference":[{"key":"3361_CR1","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1586\/17476348.2015.1027689","volume":"9","author":"JA Al-Tawfiq","year":"2015","unstructured":"Al-Tawfiq JA, Memish ZA (2015) An update on Middle East respiratory syndrome: 2 years later. Expert Rev Respir Med 9:327\u2013335","journal-title":"Expert Rev Respir Med"},{"key":"3361_CR2","doi-asserted-by":"crossref","first-page":"20290","DOI":"10.2807\/ese.17.40.20290-en","volume":"17","author":"A Bermingham","year":"2012","unstructured":"Bermingham A, Chand MA, Brown CS, Aarons E, Tong C, Langrish C et al (2012) Severe respiratory illness caused by a novel coronavirus, in a patient transferred to the United Kingdom from the Middle East, September 2012. Euro Surveill 17:20290","journal-title":"Euro Surveill"},{"key":"3361_CR3","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.3201\/eid2006.140299","volume":"20","author":"DK Chu","year":"2014","unstructured":"Chu DK, Poon LL, Gomaa MM, Shehata MM, Perera RA, Abu ZD et al (2014) MERS coronaviruses in dromedary camels, Egypt. Emerg Infect Dis 20:1049\u20131053","journal-title":"Emerg Infect Dis"},{"key":"3361_CR4","doi-asserted-by":"crossref","first-page":"1819","DOI":"10.3201\/eid1911.131172","volume":"19","author":"ZA Memish","year":"2013","unstructured":"Memish ZA, Mishra N, Olival KJ, Fagbo SF, Kapoor V, Epstein JH et al (2013) Middle East respiratory syndrome coronavirus in bats, Saudi Arabia. Emerg Infect Dis 19:1819\u20131823","journal-title":"Emerg Infect Dis"},{"key":"3361_CR5","doi-asserted-by":"crossref","first-page":"1697","DOI":"10.3201\/eid1910.130946","volume":"19","author":"NL Ithete","year":"2013","unstructured":"Ithete NL, Stoffberg S, Corman VM, Cottontail VM, Richards LR, Schoeman MC et al (2013) Close relative of human Middle East respiratory syndrome coronavirus in bat, South Africa. Emerg Infect Dis 19:1697\u20131699","journal-title":"Emerg Infect Dis"},{"key":"3361_CR6","first-page":"1360","volume":"37","author":"TA Madani","year":"2014","unstructured":"Madani TA, Azhar EI, Hashem AM (2014) Evidence for camel-to-human transmission of MERS coronavirus. N Engl J Med 37:1360","journal-title":"N Engl J Med"},{"key":"3361_CR7","doi-asserted-by":"crossref","first-page":"699","DOI":"10.3201\/eid2104.141949","volume":"21","author":"MG Hemida","year":"2015","unstructured":"Hemida MG, Al-Naeem A, Perera RA, Chin AW, Poon LL, Peiris M (2015) Lack of middle East respiratory syndrome coronavirus transmission from infected camels. Emerg Infect Dis 21:699\u2013701","journal-title":"Emerg Infect Dis"},{"key":"3361_CR8","doi-asserted-by":"crossref","first-page":"1814","DOI":"10.1056\/NEJMoa1211721","volume":"367","author":"AM Zaki","year":"2012","unstructured":"Zaki AM, van Boheemen S, Bestebroer TM, Osterhaus AD, Fouchier RA (2012) Isolation of a novel coronavirus from a man with pneumonia in Saudi Arabia. N Engl J Med 367:1814\u20131820","journal-title":"N Engl J Med"},{"key":"3361_CR9","doi-asserted-by":"crossref","first-page":"1993","DOI":"10.1016\/S0140-6736(13)61887-5","volume":"382","author":"M Cotten","year":"2013","unstructured":"Cotten M, Watson SJ, Kellam P, Al-Rabeeah AA, Makhdoom HQ, Assiri A et al (2013) Transmission and evolution of the Middle East respiratory syndrome coronavirus in Saudi Arabia: a descriptive genomic study. Lancet 382:1993\u20132002","journal-title":"Lancet"},{"key":"3361_CR10","first-page":"71","volume":"132","author":"TG Burland","year":"2000","unstructured":"Burland TG (2000) DNASTAR\u2019s Lasergene sequence analysis software. Methods Mol Biol 132:71\u201391","journal-title":"Methods Mol Biol"},{"key":"3361_CR11","doi-asserted-by":"crossref","first-page":"2725","DOI":"10.1093\/molbev\/mst197","volume":"30","author":"K Tamura","year":"2013","unstructured":"Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol 30:2725\u20132729","journal-title":"Mol Biol Evol"},{"key":"3361_CR12","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1093\/bioinformatics\/17.7.646","volume":"17","author":"S M\u00f6ller","year":"2001","unstructured":"M\u00f6ller S, Croning MD, Apweiler R (2001) Evaluation of methods for the prediction of membrane spanning regions. Bioinformatics 17:646\u2013653","journal-title":"Bioinformatics"},{"key":"3361_CR13","doi-asserted-by":"crossref","first-page":"1172","DOI":"10.1093\/bioinformatics\/btl023","volume":"22","author":"IL Hofacker","year":"2006","unstructured":"Hofacker IL, Stadler PF (2006) Memory efficient folding algorithms for circular RNA secondary structures. Bioinformatics 22:1172\u20131176","journal-title":"Bioinformatics"},{"key":"3361_CR14","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1007\/s11427-015-4903-7","volume":"58","author":"Q Xie","year":"2015","unstructured":"Xie Q, Cao Y, Su J, Wu X, Wan C, Ke C et al (2015) Genomic sequencing and analysis of the first imported Middle East Respiratory Syndrome Coronavirus (MERS CoV) in China. Sci China Life Sci 58:818\u2013820","journal-title":"Sci China Life Sci"},{"key":"3361_CR15","doi-asserted-by":"crossref","first-page":"9939","DOI":"10.1128\/JVI.01048-13","volume":"87","author":"L Du","year":"2013","unstructured":"Du L, Zhao G, Kou Z, Ma C, Sun S, Poon VK et al (2013) Identification of a receptor-binding domain in the S protein of the novel human coronavirus Middle East respiratory syndrome coronavirus as an essential target for vaccine development. J Virol 87:9939\u20139942","journal-title":"J Virol"},{"key":"3361_CR16","doi-asserted-by":"crossref","first-page":"13134","DOI":"10.1128\/JVI.02433-13","volume":"87","author":"J Gao","year":"2013","unstructured":"Gao J, Lu G, Qi J, Li Y, Wu Y, Deng Y et al (2013) Structure of the fusion core and inhibition of fusion by a heptad repeat peptide derived from the S protein of Middle East respiratory syndrome coronavirus. J Virol 87:13134\u201313140","journal-title":"J Virol"},{"key":"3361_CR17","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1093\/infdis\/jiu407","volume":"211","author":"S Gierer","year":"2015","unstructured":"Gierer S, Muller MA, Heurich A, Ritz D, Springstein BL, Karsten CB et al (2015) Inhibition of proprotein convertases abrogates processing of the middle eastern respiratory syndrome coronavirus spike protein in infected cells but does not reduce viral infectivity. J Infect Dis 211:889\u2013897","journal-title":"J Infect Dis"},{"key":"3361_CR18","doi-asserted-by":"crossref","first-page":"2100","DOI":"10.1016\/j.vaccine.2014.02.004","volume":"32","author":"C Ma","year":"2014","unstructured":"Ma C, Li Y, Wang L, Zhao G, Tao X, Tseng CT et al (2014) Intranasal vaccination with recombinant receptor-binding domain of MERS-CoV spike protein induces much stronger local mucosal immune responses than subcutaneous immunization: Implication for designing novel mucosal MERS vaccines. Vaccine 32:2100\u20132108","journal-title":"Vaccine"},{"key":"3361_CR19","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/j.virusres.2014.10.007","volume":"194","author":"S Xia","year":"2014","unstructured":"Xia S, Liu Q, Wang Q, Sun Z, Su S, Du L et al (2014) Middle East respiratory syndrome coronavirus (MERS-CoV) entry inhibitors targeting spike protein. Virus Res 194:200\u2013210","journal-title":"Virus Res"},{"key":"3361_CR20","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.virusres.2015.02.023","volume":"201","author":"W Surya","year":"2015","unstructured":"Surya W, Li Y, Verdia-Baguena C, Aguilella VM, Torres J (2015) MERS coronavirus envelope protein has a single transmembrane domain that forms pentameric ion channels. Virus Res 201:61\u201366","journal-title":"Virus Res"},{"key":"3361_CR21","doi-asserted-by":"publisher","first-page":"e1004320","DOI":"10.1371\/journal.ppat.1004320","volume":"10","author":"JM Jimenez-Guardeno","year":"2014","unstructured":"Jimenez-Guardeno JM, Nieto-Torres JL, DeDiego ML, Regla-Nava JA, Fernandez-Delgado R, Castano-Rodriguez C et al (2014) The PDZ-binding motif of severe acute respiratory syndrome coronavirus envelope protein is a determinant of viral pathogenesis. PloS Pathog 10:e1004320. doi: 10.1371\/journal.ppat.1004320","journal-title":"PloS Pathog"},{"key":"3361_CR22","doi-asserted-by":"publisher","first-page":"e1004077","DOI":"10.1371\/journal.ppat.1004077","volume":"10","author":"JL Nieto-Torres","year":"2014","unstructured":"Nieto-Torres JL, DeDiego ML, Verdia-Baguena C, Jimenez-Guardeno JM, Regla-Nava JA, Fernandez-Delgado R et al (2014) Severe acute respiratory syndrome coronavirus envelope protein ion channel activity promotes virus fitness and pathogenesis. PloS Pathog 10:e1004077. doi: 10.1371\/journal.ppat.1004077","journal-title":"PloS Pathog"},{"key":"3361_CR23","doi-asserted-by":"publisher","first-page":"e1002674","DOI":"10.1371\/journal.ppat.1002674","volume":"8","author":"TR Ruch","year":"2012","unstructured":"Ruch TR, Machamer CE (2012) A single polar residue and distinct membrane topologies impact the function of the infectious bronchitis coronavirus E protein. PLoS Pathog 8:e1002674. doi: 10.1371\/journal.ppat.1002674","journal-title":"PLoS Pathog"},{"key":"3361_CR24","doi-asserted-by":"crossref","first-page":"3870","DOI":"10.1128\/JVI.03566-14","volume":"89","author":"JA Regla-Nava","year":"2015","unstructured":"Regla-Nava JA, Nieto-Torres JL, Jimenez-Guardeno JM, Fernandez-Delgado R, Fett C, Castano-Rodriguez C et al (2015) Severe acute respiratory syndrome coronaviruses with mutations in the E protein are attenuated and promising vaccine candidates. J Virol 89:3870\u20133887","journal-title":"J Virol"},{"key":"3361_CR25","doi-asserted-by":"publisher","first-page":"e00650-13","DOI":"10.1128\/mBio.00650-13","volume":"5","author":"F Almaz\u00e1n","year":"2013","unstructured":"Almaz\u00e1n F, DeDiego ML, Sola I, Zu\u00f1iga S, Nieto-Torres JL, Marquez-Jurado S et al (2013) Engineering a replication-competent, propagation-defective Middle East respiratory syndrome coronavirus as a vaccine candidate. MBio 5:e00650-13. doi: 10.1128\/mBio.00650-13","journal-title":"MBio"},{"key":"3361_CR26","doi-asserted-by":"crossref","first-page":"16157","DOI":"10.1073\/pnas.1311542110","volume":"111","author":"T Scobey","year":"2013","unstructured":"Scobey T, Yount BL, Sims AC, Donaldson EF, Agnihothram SS, Menachery VD et al (2013) Reverse genetics with a full-length infectious cDNA of the Middle East respiratory syndrome coronavirus. Proc Natl Acad Sci USA 111:16157\u201316162","journal-title":"Proc Natl Acad Sci USA"},{"key":"3361_CR27","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1099\/0022-1317-81-3-791","volume":"81","author":"K Stirrups","year":"2000","unstructured":"Stirrups K, Shaw K, Evans S, Dalton K, Cavanagh D, Britton P (2000) Leader switching occurs during the rescue of defective RNAs by heterologous strains of the coronavirus infectious bronchitis virus. J Gen Virol 81:791\u2013801","journal-title":"J Gen Virol"},{"key":"3361_CR28","doi-asserted-by":"crossref","first-page":"2720","DOI":"10.1128\/JVI.70.5.2720-2729.1996","volume":"70","author":"RY Chang","year":"1996","unstructured":"Chang RY, Krishnan R, Brian DA (1996) The UCUAAAC promoter motif is not required for high-frequency leader recombination in bovine coronavirus defective interfering RNA. J Virol 70:2720\u20132729","journal-title":"J Virol"},{"key":"3361_CR29","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1016\/0022-2836(86)90270-6","volume":"192","author":"S Kuge","year":"1986","unstructured":"Kuge S, Saito I, Nomoto A (1986) Primary structure of poliovirus defective-interfering particle genomes and possible generation mechanisms of the particles. J Mol Biol 192:473\u2013487","journal-title":"J Mol Biol"},{"key":"3361_CR30","doi-asserted-by":"crossref","first-page":"7851","DOI":"10.1128\/JVI.69.12.7851-7856.1995","volume":"69","author":"G Marle van","year":"1995","unstructured":"van Marle G, Luytjes W, van der Most RG, van der Straaten T, Spaan WJ (1995) Regulation of coronavirus mRNA transcription. J Virol 69:7851\u20137856","journal-title":"J Virol"},{"key":"3361_CR31","doi-asserted-by":"crossref","first-page":"3428","DOI":"10.1099\/vir.0.83284-0","volume":"88","author":"E Maori","year":"2007","unstructured":"Maori E, Lavi S, Mozes-Koch R, Gantman Y, Peretz Y, Edelbaum O et al (2007) Isolation and characterization of Israeli acute paralysis virus, a dicistrovirus affecting honeybees in Israel: evidence for diversity due to intra- and inter-species recombination. J Gen Virol 88:3428\u20133438","journal-title":"J Gen Virol"},{"key":"3361_CR32","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1016\/j.virol.2011.03.029","volume":"415","author":"JL Nieto-Torres","year":"2011","unstructured":"Nieto-Torres JL, Dediego ML, Alvarez E, Jim\u00e9nez-Guarde\u00f1o JM, Regla-Nava JA, Llorente M et al (2011) Subcellular location and topology of severe acute respiratory syndrome coronavirus envelope protein. Virology 415:69\u201382. doi: 10.1016\/j.virol.2011.03.029","journal-title":"Virology"},{"key":"3361_CR33","doi-asserted-by":"crossref","first-page":"9313","DOI":"10.1128\/JVI.01237-15","volume":"89","author":"JW Westerbeck","year":"2015","unstructured":"Westerbeck JW, Machamer CE (2015) A coronavirus E protein is present in two distinct pools with different effects on assembly and the secretory pathway. J Virol 89:9313\u20139323","journal-title":"J Virol"}],"container-title":["Archives of Virology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00705-017-3361-x\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00705-017-3361-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00705-017-3361-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,7,27]],"date-time":"2022-07-27T15:35:18Z","timestamp":1658936118000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00705-017-3361-x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,4,18]]},"references-count":33,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2017,8]]}},"alternative-id":["3361"],"URL":"https:\/\/doi.org\/10.1007\/s00705-017-3361-x","relation":{},"ISSN":["0304-8608","1432-8798"],"issn-type":[{"value":"0304-8608","type":"print"},{"value":"1432-8798","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,4,18]]}}}