{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T17:41:53Z","timestamp":1791394913972,"version":"4.3.3"},"reference-count":27,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2019,3,11]],"date-time":"2019-03-11T00:00:00Z","timestamp":1552262400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2019,3,11]],"date-time":"2019-03-11T00:00:00Z","timestamp":1552262400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31602095"],"award-info":[{"award-number":["31602095"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program","doi-asserted-by":"crossref","award":["2016YFD0501505"],"award-info":[{"award-number":["2016YFD0501505"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"name":"China Agriculture Research System","award":["CARS-35"],"award-info":[{"award-number":["CARS-35"]}]},{"name":"Central Public Interest Scientific Institution Basal Research Fund","award":["Y2016CG23"],"award-info":[{"award-number":["Y2016CG23"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Arch Virol"],"published-print":{"date-parts":[[2019,5,1]]},"DOI":"10.1007\/s00705-019-04167-3","type":"journal-article","created":{"date-parts":[[2019,3,11]],"date-time":"2019-03-11T14:29:45Z","timestamp":1552314585000},"page":"1287-1295","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Biological characterization and pathogenicity of a newly isolated Chinese highly virulent genotype GIIa porcine epidemic diarrhea virus strain"],"prefix":"10.1007","volume":"164","author":[{"given":"Liping","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinsheng","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiaoling","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuzhen","family":"Fang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoliang","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donghong","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiyan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxin","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongguang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6418-3913","authenticated-orcid":false,"given":"Yonglu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,3,11]]},"reference":[{"key":"4167_CR1","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.12991","author":"J Guo","year":"2018","unstructured":"Guo J, Fang L, Ye X, Chen J, Xu S, Zhu X, Miao Y, Wang D, Xiao S (2018) Evolutionary and genotypic analyses of global porcine epidemic diarrhea virus strains. Transbound Emerg Dis. https:\/\/doi.org\/10.1111\/tbed.12991","journal-title":"Transbound Emerg Dis"},{"issue":"3","key":"4167_CR2","doi-asserted-by":"publisher","first-page":"355","DOI":"10.1292\/jvms.15-0446","volume":"78","author":"D Sun","year":"2016","unstructured":"Sun D, Wang X, Wei S, Chen J, Feng L (2016) Epidemiology and vaccine of porcine epidemic diarrhea virus in China: a mini-review. J Vet Med Sci 78(3):355\u2013363. https:\/\/doi.org\/10.1292\/jvms.15-0446","journal-title":"J Vet Med Sci"},{"issue":"3","key":"4167_CR3","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1007\/BF01317606","volume":"58","author":"MB Pensaert","year":"1978","unstructured":"Pensaert MB, de Bouck P (1978) A new coronavirus-like particle associated with diarrhea in swine. Arch Virol 58(3):243\u2013247","journal-title":"Arch Virol"},{"key":"4167_CR4","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1016\/j.virusres.2016.09.003","volume":"226","author":"B Choudhury","year":"2016","unstructured":"Choudhury B, Dastjerdi A, Doyle N, Frossard JP, Steinbach F (2016) From the field to the lab\u2014an European view on the global spread of PEDV. Virus Res 226:40\u201349. https:\/\/doi.org\/10.1016\/j.virusres.2016.09.003","journal-title":"Virus Res"},{"key":"4167_CR5","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1016\/j.virusres.2016.05.023","volume":"226","author":"CM Lin","year":"2016","unstructured":"Lin CM, Saif LJ, Marthaler D, Wang Q (2016) Evolution, antigenicity and pathogenicity of global porcine epidemic diarrhea virus strains. Virus Res 226:20\u201339. https:\/\/doi.org\/10.1016\/j.virusres.2016.05.023","journal-title":"Virus Res"},{"issue":"9","key":"4167_CR6","doi-asserted-by":"publisher","first-page":"2601","DOI":"10.1007\/s00705-016-2938-0","volume":"161","author":"R Paraguison-Alili","year":"2016","unstructured":"Paraguison-Alili R, Domingo CY (2016) Phylogenetic tracking of current porcine epidemic diarrhea virus (PEDV) strains in the Philippines. Arch Virol 161(9):2601\u20132604. https:\/\/doi.org\/10.1007\/s00705-016-2938-0","journal-title":"Arch Virol"},{"key":"4167_CR7","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1186\/1743-422X-9-195","volume":"9","author":"Y Pan","year":"2012","unstructured":"Pan Y, Tian X, Li W, Zhou Q, Wang D, Bi Y, Chen F, Song Y (2012) Isolation and characterization of a variant porcine epidemic diarrhea virus in China. Virol J 9:195. https:\/\/doi.org\/10.1186\/1743-422X-9-195","journal-title":"Virol J"},{"issue":"12","key":"4167_CR8","doi-asserted-by":"publisher","first-page":"2089","DOI":"10.3201\/eid2012.131642","volume":"20","author":"S Park","year":"2014","unstructured":"Park S, Kim S, Song D, Park B (2014) Novel porcine epidemic diarrhea virus variant with large genomic deletion, South Korea. Emerg Infect Dis 20(12):2089\u20132092. https:\/\/doi.org\/10.3201\/eid2012.131642","journal-title":"Emerg Infect Dis"},{"key":"4167_CR9","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1016\/j.rvsc.2015.09.024","volume":"103","author":"R Yamamoto","year":"2015","unstructured":"Yamamoto R, Soma J, Nakanishi M, Yamaguchi R, Niinuma S (2015) Isolation and experimental inoculation of an S INDEL strain of porcine epidemic diarrhea virus in Japan. Res Vet Sci 103:103\u2013106. https:\/\/doi.org\/10.1016\/j.rvsc.2015.09.024","journal-title":"Res Vet Sci"},{"issue":"8","key":"4167_CR10","doi-asserted-by":"publisher","first-page":"1931","DOI":"10.1007\/s00705-015-2463-6","volume":"160","author":"DT Vui","year":"2015","unstructured":"Vui DT, Thanh TL, Tung N, Srijangwad A, Tripipat T, Chuanasa T, Nilubol D (2015) Complete genome characterization of porcine epidemic diarrhea virus in Vietnam. Arch Virol 160(8):1931\u20131938. https:\/\/doi.org\/10.1007\/s00705-015-2463-6","journal-title":"Arch Virol"},{"issue":"7","key":"4167_CR11","doi-asserted-by":"publisher","first-page":"1112","DOI":"10.3201\/eid1507.081256","volume":"15","author":"S Puranaveja","year":"2009","unstructured":"Puranaveja S, Poolperm P, Lertwatcharasarakul P, Kesdaengsakonwut S, Boonsoongnern A, Urairong K, Kitikoon P, Choojai P, Kedkovid R, Teankum K, Thanawongnuwech R (2009) Chinese-like strain of porcine epidemic diarrhea virus, Thailand. Emerg Infect Dis 15(7):1112\u20131115. https:\/\/doi.org\/10.3201\/eid1507.081256","journal-title":"Emerg Infect Dis"},{"key":"4167_CR12","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1016\/j.virusres.2016.08.005","volume":"226","author":"K Crawford","year":"2016","unstructured":"Crawford K, Lager KM, Kulshreshtha V, Miller LC, Faaberg KS (2016) Status of vaccines for porcine epidemic diarrhea virus in the United States and Canada. Virus Res 226:108\u2013116. https:\/\/doi.org\/10.1016\/j.virusres.2016.08.005","journal-title":"Virus Res"},{"issue":"2","key":"4167_CR13","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1007\/s11262-017-1528-x","volume":"54","author":"R Lara-Romero","year":"2018","unstructured":"Lara-Romero R, Gomez-Nunez L, Cerriteno-Sanchez JL, Marquez-Valdelamar L, Mendoza-Elvira S, Ramirez-Mendoza H, Rivera-Benitez JF (2018) Molecular characterization of the spike gene of the porcine epidemic diarrhea virus in Mexico, 2013\u20132016. Virus Genes 54(2):215\u2013224. https:\/\/doi.org\/10.1007\/s11262-017-1528-x","journal-title":"Virus Genes"},{"key":"4167_CR14","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1016\/j.prevetmed.2015.10.020","volume":"123","author":"MC Jarvis","year":"2016","unstructured":"Jarvis MC, Lam HC, Zhang Y, Wang L, Hesse RA, Hause BM, Vlasova A, Wang Q, Zhang J, Nelson MI, Murtaugh MP, Marthaler D (2016) Genomic and evolutionary inferences between American and global strains of porcine epidemic diarrhea virus. Prev Vet Med 123:175\u2013184. https:\/\/doi.org\/10.1016\/j.prevetmed.2015.10.020","journal-title":"Prev Vet Med"},{"key":"4167_CR15","doi-asserted-by":"publisher","DOI":"10.1128\/genomea.00503-14","author":"PK Lawrence","year":"2014","unstructured":"Lawrence PK, Bumgardner E, Bey RF, Stine D, Bumgarner RE (2014) Genome sequences of porcine epidemic diarrhea virus: in vivo and in vitro phenotypes. Genome Announc. https:\/\/doi.org\/10.1128\/genomea.00503-14","journal-title":"Genome Announc"},{"issue":"2","key":"4167_CR16","first-page":"197","volume":"29","author":"FM Zheng","year":"2013","unstructured":"Zheng FM, Huo JY, Zhao J, Chang HT, Wang XM, Chen L, Wang CQ (2013) Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus field strains in central China during 2010\u20132012 outbreaks. Bing du xue bao Chin J Virol 29(2):197\u2013205","journal-title":"Bing du xue bao Chin J Virol"},{"key":"4167_CR17","doi-asserted-by":"publisher","DOI":"10.3390\/v10080399","author":"C Kaewborisuth","year":"2018","unstructured":"Kaewborisuth C, He Q, Jongkaewwattana A (2018) The accessory protein ORF3 contributes to porcine epidemic diarrhea virus replication by direct binding to the spike protein. Viruses. https:\/\/doi.org\/10.3390\/v10080399","journal-title":"Viruses"},{"key":"4167_CR18","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.00603-18","author":"D Wang","year":"2018","unstructured":"Wang D, Ge X, Chen D, Li J, Cai Y, Deng J, Zhou L, Guo X, Han J, Yang H (2018) The S gene is necessary but not sufficient for the virulence of porcine epidemic diarrhea virus novel variant strain BJ2011C. J Virol. https:\/\/doi.org\/10.1128\/jvi.00603-18","journal-title":"J Virol"},{"issue":"3","key":"4167_CR19","doi-asserted-by":"publisher","first-page":"1253","DOI":"10.3390\/v6031253","volume":"6","author":"D Shi","year":"2014","unstructured":"Shi D, Lv M, Chen J, Shi H, Zhang S, Zhang X, Feng L (2014) Molecular characterizations of subcellular localization signals in the nucleocapsid protein of porcine epidemic diarrhea virus. Viruses 6(3):1253\u20131273. https:\/\/doi.org\/10.3390\/v6031253","journal-title":"Viruses"},{"issue":"3","key":"4167_CR20","doi-asserted-by":"publisher","first-page":"e21","DOI":"10.4110\/in.2018.18.e21","volume":"18","author":"SH Kim","year":"2018","unstructured":"Kim SH, Cho BH, Lee KY, Jang YS (2018) N-terminal domain of the spike protein of porcine epidemic diarrhea virus as a new candidate molecule for a mucosal vaccine. Immune Netw 18(3):e21. https:\/\/doi.org\/10.4110\/in.2018.18.e21","journal-title":"Immune Netw"},{"issue":"2","key":"4167_CR21","doi-asserted-by":"publisher","first-page":"e00165-14","DOI":"10.1128\/genomeA.00165-14","volume":"2","author":"A Jia","year":"2014","unstructured":"Jia A, Feng X, Liu Q, Zhou R, Wang G (2014) Complete genome sequence of CHYJ130330, a highly virulent strain of porcine epidemic diarrhea virus in South China. Genome Announc 2(2):e00165-14. https:\/\/doi.org\/10.1128\/genomea.00165-14","journal-title":"Genome Announc"},{"issue":"8","key":"4167_CR22","doi-asserted-by":"publisher","first-page":"1350","DOI":"10.3201\/eid1803.120002","volume":"18","author":"W Li","year":"2012","unstructured":"Li W, Li H, Liu Y, Pan Y, Deng F, Song Y, Tang X, He Q (2012) New variants of porcine epidemic diarrhea virus, China, 2011. Emerg Infect Dis 18(8):1350\u20131353. https:\/\/doi.org\/10.3201\/eid1808.120002","journal-title":"Emerg Infect Dis"},{"issue":"1","key":"4167_CR23","doi-asserted-by":"publisher","first-page":"194","DOI":"10.1186\/s12985-017-0860-z","volume":"14","author":"Q Zhang","year":"2017","unstructured":"Zhang Q, Liu X, Fang Y, Zhou P, Wang Y, Zhang Y (2017) Detection and phylogenetic analyses of spike genes in porcine epidemic diarrhea virus strains circulating in China in 2016-2017. Virol J 14(1):194. https:\/\/doi.org\/10.1186\/s12985-017-0860-z","journal-title":"Virol J"},{"issue":"3\u20134","key":"4167_CR24","doi-asserted-by":"publisher","first-page":"258","DOI":"10.1016\/j.vetmic.2014.08.012","volume":"173","author":"T Oka","year":"2014","unstructured":"Oka T, Saif LJ, Marthaler D, Esseili MA, Meulia T, Lin CM, Vlasova AN, Jung K, Zhang Y, Wang Q (2014) Cell culture isolation and sequence analysis of genetically diverse US porcine epidemic diarrhea virus strains including a novel strain with a large deletion in the spike gene. Vet Microbiol 173(3\u20134):258\u2013269. https:\/\/doi.org\/10.1016\/j.vetmic.2014.08.012","journal-title":"Vet Microbiol"},{"key":"4167_CR25","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1186\/s13567-015-0249-1","volume":"46","author":"X Liu","year":"2015","unstructured":"Liu X, Lin CM, Annamalai T, Gao X, Lu Z, Esseili MA, Jung K, El-Tholoth M, Saif LJ, Wang Q (2015) Determination of the infectious titer and virulence of an original US porcine epidemic diarrhea virus PC22A strain. Vet Res 46:109. https:\/\/doi.org\/10.1186\/s13567-015-0249-1","journal-title":"Vet Res"},{"key":"4167_CR26","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1016\/j.virusres.2016.05.026","volume":"226","author":"D Wang","year":"2016","unstructured":"Wang D, Fang L, Xiao S (2016) Porcine epidemic diarrhea in China. Virus Res 226:7\u201313. https:\/\/doi.org\/10.1016\/j.virusres.2016.05.026","journal-title":"Virus Res"},{"key":"4167_CR27","doi-asserted-by":"publisher","first-page":"50","DOI":"10.1016\/j.virol.2016.10.011","volume":"500","author":"B Fan","year":"2017","unstructured":"Fan B, Yu Z, Pang F, Xu X, Zhang B, Guo R, He K, Li B (2017) Characterization of a pathogenic full-length cDNA clone of a virulent porcine epidemic diarrhea virus strain AH2012\/12 in China. Virology 500:50\u201361. https:\/\/doi.org\/10.1016\/j.virol.2016.10.011","journal-title":"Virology"}],"container-title":["Archives of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00705-019-04167-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00705-019-04167-3\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00705-019-04167-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T05:19:40Z","timestamp":1744175980000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00705-019-04167-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,11]]},"references-count":27,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2019,5,1]]}},"alternative-id":["4167"],"URL":"https:\/\/doi.org\/10.1007\/s00705-019-04167-3","relation":{},"ISSN":["0304-8608","1432-8798"],"issn-type":[{"value":"0304-8608","type":"print"},{"value":"1432-8798","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3,11]]},"assertion":[{"value":"10 November 2018","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 January 2019","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 March 2019","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"The authors declare that they have no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"All piglets used in the present study were humanely bred during the experiment and euthanized at the end of the experiment. Animal care and use protocols were reviewed and approved by the Institutional Animal Use and Care Committee of Lanzhou Veterinary Research Institute.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}}]}}