{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T05:11:09Z","timestamp":1788930669311,"version":"build-2803163510"},"reference-count":18,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2020,11,27]],"date-time":"2020-11-27T00:00:00Z","timestamp":1606435200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2020,11,27]],"date-time":"2020-11-27T00:00:00Z","timestamp":1606435200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Arch Virol"],"published-print":{"date-parts":[[2021,2]]},"DOI":"10.1007\/s00705-020-04892-0","type":"journal-article","created":{"date-parts":[[2020,11,27]],"date-time":"2020-11-27T12:03:54Z","timestamp":1606478634000},"page":"587-591","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Use of whole blood over plasma enhances the detection of dengue virus RNA: possible utility in dengue vaccine trials"],"prefix":"10.1007","volume":"166","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1401-8589","authenticated-orcid":false,"given":"K.","family":"Alagarasu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. B.","family":"Kakade","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. V.","family":"Bachal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Bote","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Parashar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. S.","family":"Shah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,11,27]]},"reference":[{"key":"4892_CR1","doi-asserted-by":"publisher","first-page":"S89","DOI":"10.1093\/infdis\/jiw649","volume":"215","author":"DA Muller","year":"2017","unstructured":"Muller DA, Depelsenaire ACI, Young PR (2017) Clinical and laboratory diagnosis of dengue virus infection. J Infect Dis 215:S89\u2013S95. https:\/\/doi.org\/10.1093\/infdis\/jiw649","journal-title":"J Infect Dis"},{"issue":"9","key":"4892_CR2","doi-asserted-by":"publisher","first-page":"e0004100","DOI":"10.1371\/journal.pntd.0004100","volume":"9","author":"AC Andries","year":"2015","unstructured":"Andries AC, Duong V, Ly S et al (2015) Value of routine dengue diagnostic tests in urine and saliva specimens. PLoS Negl Trop Dis 9(9):e0004100. https:\/\/doi.org\/10.1371\/journal.pntd.0004100","journal-title":"PLoS Negl Trop Dis"},{"key":"4892_CR3","doi-asserted-by":"publisher","first-page":"1200","DOI":"10.1016\/j.vaccine.2016.10.070","volume":"35","author":"World Health Organization null","year":"2017","unstructured":"World Health Organization null (2017) Dengue vaccine: WHO position paper, July 2016\u2014recommendations. Vaccine 35:1200\u20131201. https:\/\/doi.org\/10.1016\/j.vaccine.2016.10.070","journal-title":"Vaccine"},{"key":"4892_CR4","unstructured":"World Health Organization (2012) Guidelines on the quality, safety and efficacy of dengue tetravalent vaccines (live, attenuated). www.who.int\/biologicals\/vaccines\/Dengue_DB_5_April_2012_clean.pdf. Accessed 1 Oct 2020."},{"key":"4892_CR5","doi-asserted-by":"publisher","first-page":"2311","DOI":"10.1007\/s00705-020-04725-0","volume":"165","author":"MB Kakade","year":"2020","unstructured":"Kakade MB, Shrivastava N, Patil JA et al (2020) Clinical evaluation of an in-house-developed real-time RT-PCR assay for serotyping of dengue virus. Arch Virol 165:2311\u20132315. https:\/\/doi.org\/10.1007\/s00705-020-04725-0","journal-title":"Arch Virol"},{"key":"4892_CR6","doi-asserted-by":"publisher","first-page":"71","DOI":"10.4103\/ijmr.IJMR_613_18","volume":"151","author":"R Kulkarni","year":"2020","unstructured":"Kulkarni R, Modak M, Gosavi M et al (2020) Comparative assessment of commercial enzyme-linked immunosorbent assay and rapid diagnostic tests used for dengue diagnosis in India. Indian J Med Res 151:71\u201378. https:\/\/doi.org\/10.4103\/ijmr.IJMR_613_18","journal-title":"Indian J Med Res"},{"key":"4892_CR7","doi-asserted-by":"publisher","first-page":"2480","DOI":"10.1128\/JCM.00305-07","volume":"45","author":"C Klungthong","year":"2007","unstructured":"Klungthong C, Gibbons RV, Thaisomboonsuk B et al (2007) Dengue virus detection using whole blood for reverse transcriptase PCR and virus isolation. J Clin Microbiol 45:2480\u20132485. https:\/\/doi.org\/10.1128\/JCM.00305-07","journal-title":"J Clin Microbiol"},{"key":"4892_CR8","doi-asserted-by":"publisher","first-page":"S25","DOI":"10.1016\/j.ijid.2018.12.003","volume":"84S","author":"M Kar","year":"2019","unstructured":"Kar M, Nisheetha A, Kumar A et al (2019) Isolation and molecular characterization of dengue virus clinical isolates from pediatric patients in New Delhi. Int J Infect Dis 84S:S25\u2013S33. https:\/\/doi.org\/10.1016\/j.ijid.2018.12.003","journal-title":"Int J Infect Dis"},{"key":"4892_CR9","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1016\/s0166-0934(02)00005-8","volume":"102","author":"SO De Paula","year":"2002","unstructured":"De Paula SO, Lopes da Fonseca BA (2002) Optimizing dengue diagnosis by RT-PCR in IgM-positive samples: comparison of whole blood, buffy-coat and serum as clinical samples. J Virol Methods 102:113\u2013117. https:\/\/doi.org\/10.1016\/s0166-0934(02)00005-8","journal-title":"J Virol Methods"},{"key":"4892_CR10","doi-asserted-by":"publisher","first-page":"297","DOI":"10.1016\/j.jcv.2003.11.004","volume":"30","author":"SO De Paula","year":"2004","unstructured":"De Paula SO, de Melo LC, Torres MP et al (2004) One-step RT-PCR protocols improve the rate of dengue diagnosis compared to two-step RT-PCR approaches. J Clin Virol 30:297\u2013301. https:\/\/doi.org\/10.1016\/j.jcv.2003.11.004","journal-title":"J Clin Virol"},{"key":"4892_CR11","doi-asserted-by":"publisher","first-page":"827","DOI":"10.1111\/1462-2920.14516","volume":"21","author":"F Cholet","year":"2019","unstructured":"Cholet F, Ijaz UZ, Smith CJ (2019) Differential ratio amplicons (Ramp) for the evaluation of RNA integrity extracted from complex environmental samples. Environ Microbiol 21:827\u2013844. https:\/\/doi.org\/10.1111\/1462-2920.14516","journal-title":"Environ Microbiol"},{"key":"4892_CR12","doi-asserted-by":"publisher","first-page":"e0004445","DOI":"10.1371\/journal.pntd.0004445","volume":"10","author":"G A\u00f1ez","year":"2016","unstructured":"A\u00f1ez G, Heisey DAR, Chancey C et al (2016) Distribution of dengue virus types 1 and 4 in blood components from infected blood donors from Puerto Rico. PLoS Negl Trop Dis 10:e0004445. https:\/\/doi.org\/10.1371\/journal.pntd.0004445","journal-title":"PLoS Negl Trop Dis"},{"key":"4892_CR13","doi-asserted-by":"publisher","first-page":"360","DOI":"10.1186\/s12879-015-1088-4","volume":"15","author":"X Shan","year":"2015","unstructured":"Shan X, Wang X, Yuan Q et al (2015) Evaluation of the diagnostic accuracy of nonstructural protein 1 Ag-based tests for dengue virus in Asian population: a meta-analysis. BMC Infect Dis 15:360. https:\/\/doi.org\/10.1186\/s12879-015-1088-4","journal-title":"BMC Infect Dis"},{"key":"4892_CR14","unstructured":"World Health Organization (2009) Dengue guidelines for diagnosis, treatment, prevention and control: new edition. World Health Organization. https:\/\/apps.who.int\/iris\/handle\/10665\/44188. Accessed 27 July 2020"},{"key":"4892_CR15","doi-asserted-by":"publisher","first-page":"2707","DOI":"10.1016\/j.vaccine.2016.04.028","volume":"34","author":"E Plennevaux","year":"2016","unstructured":"Plennevaux E, Sabchareon A, Limkittikul K et al (2016) Detection of dengue cases by serological testing in a dengue vaccine efficacy trial: utility for efficacy evaluation and impact of future vaccine introduction. Vaccine 34:2707\u20132712. https:\/\/doi.org\/10.1016\/j.vaccine.2016.04.028","journal-title":"Vaccine"},{"key":"4892_CR16","doi-asserted-by":"publisher","first-page":"1164","DOI":"10.1093\/cid\/cix966","volume":"66","author":"E Plennevaux","year":"2018","unstructured":"Plennevaux E, Moureau A, Arredondo-Garc\u00eda JL et al (2018) Impact of dengue vaccination on serological diagnosis: insights from phase III dengue vaccine efficacy trials. Clin Infect Dis 66:1164\u20131172. https:\/\/doi.org\/10.1093\/cid\/cix966","journal-title":"Clin Infect Dis"},{"key":"4892_CR17","doi-asserted-by":"publisher","first-page":"2759","DOI":"10.1016\/j.vaccine.2016.04.070","volume":"34","author":"K Alagarasu","year":"2016","unstructured":"Alagarasu K (2016) Introducing dengue vaccine: implications for diagnosis in dengue vaccinated subjects. Vaccine 34:2759\u20132761. https:\/\/doi.org\/10.1016\/j.vaccine.2016.04.070","journal-title":"Vaccine"},{"key":"4892_CR18","doi-asserted-by":"publisher","first-page":"317","DOI":"10.3389\/fcimb.2017.00317","volume":"7","author":"C Guo","year":"2017","unstructured":"Guo C, Zhou Z, Wen Z et al (2017) Global epidemiology of dengue outbreaks in 1990\u20132015: a systematic review and meta-analysis. Front Cell Infect Microbiol 7:317. https:\/\/doi.org\/10.3389\/fcimb.2017.00317","journal-title":"Front Cell Infect Microbiol"}],"container-title":["Archives of Virology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00705-020-04892-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00705-020-04892-0\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00705-020-04892-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,2,2]],"date-time":"2021-02-02T05:01:01Z","timestamp":1612242061000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00705-020-04892-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11,27]]},"references-count":18,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2021,2]]}},"alternative-id":["4892"],"URL":"https:\/\/doi.org\/10.1007\/s00705-020-04892-0","relation":{},"ISSN":["0304-8608","1432-8798"],"issn-type":[{"value":"0304-8608","type":"print"},{"value":"1432-8798","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,11,27]]},"assertion":[{"value":"29 July 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 October 2020","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 November 2020","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"None declared.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"Approved by the Institutional Ethics committee of ICMR-NIV [NIV\/IEC\/2018\/D-8 and NIV\/IEC\/Jan\/2020\/D-6].","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}}]}}