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Environmental screening was undertaken to determine the source of contamination, which was suspected to be evaporation during polymerase chain reaction (PCR) assays while using the coronavirus disease 2019 (COVID-19) ARTIC protocol. A decontamination strategy is hereby documented to assist laboratories that may experience similar amplicon contamination. Routine molecular laboratory environmental screening as a quality control is highly recommended.<\/jats:p>","DOI":"10.1007\/s00705-022-05411-z","type":"journal-article","created":{"date-parts":[[2022,3,17]],"date-time":"2022-03-17T01:02:48Z","timestamp":1647478968000},"page":"1175-1179","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["A decontamination strategy for resolving SARS-CoV-2 amplicon contamination in a next-generation sequencing laboratory"],"prefix":"10.1007","volume":"167","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4732-2811","authenticated-orcid":false,"given":"Peter","family":"Mwangi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milton","family":"Mogotsi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayodeji","family":"Ogunbayo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teboho","family":"Mooko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wairimu","family":"Maringa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hlengiwe","family":"Sondlane","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelebogile","family":"Nkwadipo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olusesan","family":"Adelabu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip Armand","family":"Bester","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Goedhals","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5017-5584","authenticated-orcid":false,"given":"Martin","family":"Nyaga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,3,17]]},"reference":[{"issue":"6221","key":"5411_CR1","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1038\/339237a0","volume":"339","author":"SA Kwok","year":"1989","unstructured":"Kwok SA, Higuchi R (1989) Avoiding false positives with PCR. 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