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PCMV\/PRV is important in xenotransplantation, because in preclinical trials in which pig organs were transplanted into non-human primates, transmission of PCMV\/PRV was shown to be associated with significantly reduced survival of the xenotransplants. PCMV\/PRV was also transmitted in the first transplantation of a pig heart into a human patient worldwide and apparently contributed to the death of the patient. The prevalence of PCMV\/PRV in wild boars is largely unknown. In this study, we screened wild boars from several areas of northern Italy and Germany to test for the presence of PCMV\/PRV using PCR-based and Western blot assays. By Western blot analysis, 54% and 82% of Italian and German wild boars, respectively, were found to be PCMV\/PRV positive, while 36% and 60%, respectively, tested positive by real-time polymerase chain reaction (PCR). These data indicate that the virus is common in German and Italian wild boars and that the Western blot assay detected a PCMV\/PRV infection more often than did real-time PCR. The data also indicate that pigs raised for xenotransplantation should be protected from contact with materials from wild boars and commercial pigs.<\/jats:p>","DOI":"10.1007\/s00705-022-05690-6","type":"journal-article","created":{"date-parts":[[2023,1,7]],"date-time":"2023-01-07T10:24:28Z","timestamp":1673087068000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Presence of porcine cytomegalovirus, a porcine roseolovirus, in wild boars in Italy and Germany"],"prefix":"10.1007","volume":"168","author":[{"given":"Sabrina","family":"Hansen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Luisa","family":"Menandro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Franzo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ludwig","family":"Krabben","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen F.","family":"Marino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benedikt","family":"Kaufer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3244-6085","authenticated-orcid":false,"given":"Joachim","family":"Denner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,1,7]]},"reference":[{"key":"5690_CR1","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.virol.2014.05.014","volume":"460\u2013461","author":"W Gu","year":"2014","unstructured":"Gu W, Zeng N, Zhou L, Ge X, Guo X, Yang H (2014) Genomic organization and molecular characterization of porcine cytomegalovirus. 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