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For effective disease control, it is crucial to select an appropriate vaccine based on the specific genotype of BVDV. Therefore, developing a rapid and reliable assay to detect and genotype BVDV is imperative for controlling the spread of disease. In this study, we developed a TaqMan assay to detect and genotype BVDV types 1 and 2 directly in bovine serum without extraction of RNA. The direct BVDV TaqMan assay effectively detected both BVDV1 and BVDV2 with confirmed specificity and showed no cross-reactivity with any of the other viruses tested, including bovine respiratory syncytial virus, bovine coronavirus, Akabane virus, bovine herpesvirus 1, bovine parainfluenza virus 3, bovine immunodeficiency virus, and bovine leukemia virus. The assay could detect the virus in serum samples with a titer as low as 10\n                    <jats:sup>2<\/jats:sup>\n                    TCID\n                    <jats:sub>50<\/jats:sub>\n                    \/mL in two out of three trials for BVDV1 and all three trials for BVDV2, indicating that its sensitivity is equivalent to that of virus isolation. Our findings represent a significant advancement in BVDV detection and typing directly from bovine serum.\n                  <\/jats:p>","DOI":"10.1007\/s00705-024-06207-z","type":"journal-article","created":{"date-parts":[[2024,12,12]],"date-time":"2024-12-12T12:02:50Z","timestamp":1734004970000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Direct TaqMan assay for the detection and genotyping of bovine viral diarrhea virus types 1 and 2"],"prefix":"10.1007","volume":"170","author":[{"given":"Shakir","family":"Ullah","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kosuke","family":"Notsu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akatsuki","family":"Saito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamaki","family":"Okabayashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirohisa","family":"Mekata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norikazu","family":"Isoda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2066-128X","authenticated-orcid":false,"given":"Satoshi","family":"Sekiguchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,12,12]]},"reference":[{"key":"6207_CR1","doi-asserted-by":"publisher","unstructured":"Ye\u015filba\u011f K, Alpay G, Becher P (2017) Variability and global distribution of subgenotypes of bovine viral diarrhea virus. mdpi.com 9:128. https:\/\/doi.org\/10.3390\/v9060128","DOI":"10.3390\/v9060128"},{"key":"6207_CR2","doi-asserted-by":"publisher","first-page":"613","DOI":"10.1111\/tbed.12427","volume":"64","author":"S Silveira","year":"2015","unstructured":"Silveira S, Weber MN, Osena ACSM et al (2015) Genetic diversity of Brazilian bovine pestiviruses detected between 1995 and 2014. 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