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The gene expression of a select set of immune markers associated with inflammatory responses (TLR4, TLR7, TLR2-2, IL6, CCL4, CCR5, IFN\u03b3, IL2, IL17C), immune synapse (CD80), T helper cell polarisation (IL4) and effector cell receptors (CD4, CD8\u03b1 and CD14), following OR and ED primary vaccination and revaccination using the same or alternate route [ED\/ED (primary\/revaccination), OR\/OR, ED\/OR and OR\/ED] with ILTV SA2 strain in chickens were evaluated. Both ED and OR primary vaccinations were associated with increased gene expression of inflammation-associated markers in the conjunctiva and trachea, with a statistical interaction between application route and profile of immune responses. Revaccination via the alternative route elicited upregulation of more immune markers in the conjunctiva and trachea compared to other methods, while most immune markers were downregulated in the ED\/ED group, and close to baseline in the OR\/OR group. In conclusion, the immune responses in the conjunctiva and trachea following the ED and OR primary vaccination with ILTV vaccine were tissue-specific, showing increased gene expression of markers related to the inflammatory response. Revaccination using the alternative route elicited upregulation of markers associated with inflammatory responses CCR5 and TLR7 in the conjunctiva and\/or trachea. Challenge studies are necessary to confirm the role of these immune responses in protecting vaccinated birds from ILTV.<\/jats:p>","DOI":"10.1007\/s00705-026-06560-1","type":"journal-article","created":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T14:51:30Z","timestamp":1773845490000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Immune responses in chicken mucosal lymphoid tissues following oral and eye drop vaccination and revaccination with live modified vaccine of infectious laryngotracheitis virus (ILTV)"],"prefix":"10.1007","volume":"171","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4833-220X","authenticated-orcid":false,"given":"Thanh Tien","family":"Tran","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5881-2296","authenticated-orcid":false,"given":"Nicholas M.","family":"Andronicos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4870-706X","authenticated-orcid":false,"given":"Natkunam","family":"Ketheesan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0638-5533","authenticated-orcid":false,"given":"Stephen W.","family":"Walkden-Brown","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8343-8299","authenticated-orcid":false,"given":"Priscilla F.","family":"Gerber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,3,18]]},"reference":[{"key":"6560_CR1","unstructured":"ICTV (2024) Current ICTV Taxonomy Release. https:\/\/ictv.global\/taxonomy"},{"key":"6560_CR2","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1002\/9781119371199.ch5","volume-title":"Diseases of poultry","author":"M Garcia","year":"2020","unstructured":"Garcia M, Spatz S (2020) Infectious laryngotracheitis. 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