{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T07:59:14Z","timestamp":1785139154989,"version":"3.55.0"},"reference-count":58,"publisher":"Springer Science and Business Media LLC","issue":"7","license":[{"start":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T00:00:00Z","timestamp":1782172800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T00:00:00Z","timestamp":1782172800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100002888","name":"Beijing Municipal Commission of Education","doi-asserted-by":"publisher","award":["KZ202210005001"],"award-info":[{"award-number":["KZ202210005001"]}],"id":[{"id":"10.13039\/501100002888","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Arch Virol"],"published-print":{"date-parts":[[2026,7]]},"DOI":"10.1007\/s00705-026-06662-w","type":"journal-article","created":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T16:00:02Z","timestamp":1782230402000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Structural insights into DENV-2 NS2B-NS3 protease and inhibition by glutathione-coated gold nanocluster"],"prefix":"10.1007","volume":"171","author":[{"given":"Haojia","family":"Zhu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenchao","family":"Niu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuancong","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoyang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongfang","family":"Xia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sixu","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Botao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junshuai","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huai","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueyun","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyuan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Gong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1066-7071","authenticated-orcid":false,"given":"Yubai","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,6,23]]},"reference":[{"key":"6662_CR1","doi-asserted-by":"publisher","first-page":"488","DOI":"10.1038\/d41586-024-02513-3","volume":"632","author":"S Mallapaty","year":"2024","unstructured":"Mallapaty S (2024) The pathogens that could spark the next pandemic. Nature 632:488. https:\/\/doi.org\/10.1038\/d41586-024-02513-3","journal-title":"Nature"},{"key":"6662_CR2","doi-asserted-by":"publisher","first-page":"504","DOI":"10.1038\/nature12060","volume":"496","author":"S Bhatt","year":"2013","unstructured":"Bhatt S, Gething PW, Brady OJ et al (2013) The global distribution and burden of dengue. Nature 496:504\u2013507. https:\/\/doi.org\/10.1038\/nature12060","journal-title":"Nature"},{"key":"6662_CR3","doi-asserted-by":"publisher","first-page":"107940","DOI":"10.1016\/j.ijid.2025.107940","volume":"158","author":"N Haider","year":"2025","unstructured":"Haider N, Hasan MN, Onyango J et al (2025) Global dengue epidemic worsens with record 14 million cases and 9000 deaths reported in 2024. Int J Infect Dis 158:107940. https:\/\/doi.org\/10.1016\/j.ijid.2025.107940","journal-title":"Int J Infect Dis"},{"key":"6662_CR4","doi-asserted-by":"publisher","first-page":"1423","DOI":"10.1056\/NEJMra1110265","volume":"366","author":"CP Simmons","year":"2012","unstructured":"Simmons CP, Farrar JJ, Nguyen v V et al (2012) Dengue. N Engl J Med 366:1423\u20131432. https:\/\/doi.org\/10.1056\/NEJMra1110265","journal-title":"N Engl J Med"},{"key":"6662_CR5","unstructured":"World Health Organization (2009) Guidelines for diagnosis, treatment, prevention and control. WHO. https:\/\/wkc.who.int\/resources\/publications\/i\/item\/9789241547871. Accessed 25 Dec 2025"},{"key":"6662_CR6","doi-asserted-by":"publisher","unstructured":"Kalimuddin S, Chia PY, Low JG et al (2025) Dengue and severe dengue. Clin Microbiol Rev e0024424. https:\/\/doi.org\/10.1128\/cmr.00244-24","DOI":"10.1128\/cmr.00244-24"},{"key":"6662_CR7","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1056\/NEJMoa1411037","volume":"372","author":"L Villar","year":"2015","unstructured":"Villar L, Dayan GH, Arredondo-Garc\u00eda JL et al (2015) Efficacy of a Tetravalent Dengue Vaccine in Children in Latin America. N Engl J Med 372:113\u2013123. https:\/\/doi.org\/10.1056\/NEJMoa1411037","journal-title":"N Engl J Med"},{"key":"6662_CR8","doi-asserted-by":"publisher","first-page":"1358","DOI":"10.1016\/s0140-6736(14)61060-6","volume":"384","author":"MR Capeding","year":"2014","unstructured":"Capeding MR, Tran NH, Hadinegoro SRS et al (2014) Clinical efficacy and safety of a novel tetravalent dengue vaccine in healthy children in Asia: a phase 3, randomised, observer-masked, placebo-controlled trial. Lancet 384:1358\u20131365. https:\/\/doi.org\/10.1016\/s0140-6736(14)61060-6","journal-title":"Lancet"},{"key":"6662_CR9","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1056\/NEJMoa1411037","volume":"372","author":"L Villar","year":"2015","unstructured":"Villar L, Dayan GH, Arredondo-Garc\u00eda JL et al (2015) Efficacy of a tetravalent dengue vaccine in children in Latin America. N Engl J Med 372:113\u2013123. https:\/\/doi.org\/10.1056\/NEJMoa1411037","journal-title":"N Engl J Med"},{"key":"6662_CR10","doi-asserted-by":"publisher","first-page":"1234","DOI":"10.1016\/s1473-3099(24)00376-1","volume":"24","author":"ML Nogueira","year":"2024","unstructured":"Nogueira ML, Cintra MAT, Moreira JA et al (2024) Efficacy and safety of Butantan-DV in participants aged 2\u201359 years through an extended follow-up: results from a double-blind, randomised, placebo-controlled, phase 3, multicentre trial in Brazil. Lancet Infect Dis 24:1234\u20131244. https:\/\/doi.org\/10.1016\/s1473-3099(24)00376-1","journal-title":"Lancet Infect Dis"},{"key":"6662_CR11","doi-asserted-by":"publisher","first-page":"e257","DOI":"10.1016\/s2214-109x(23)00522-3","volume":"12","author":"V Tricou","year":"2024","unstructured":"Tricou V, Yu D, Reynales H et al (2024) Long-term efficacy and safety of a tetravalent dengue vaccine (TAK-003): 4\u00b75-year results from a phase 3, randomised, double-blind, placebo-controlled trial. Lancet Glob Health 12:e257\u2013e270. https:\/\/doi.org\/10.1016\/s2214-109x(23)00522-3","journal-title":"Lancet Glob Health"},{"key":"6662_CR12","doi-asserted-by":"publisher","first-page":"1423","DOI":"10.1016\/s0140-6736(20)30414-1","volume":"395","author":"S Biswal","year":"2020","unstructured":"Biswal S, Borja-Tabora C, Martinez Vargas L et al (2020) Efficacy of a tetravalent dengue vaccine in healthy children aged 4\u201316 years: a randomised, placebo-controlled, phase 3 trial. Lancet 395:1423\u20131433. https:\/\/doi.org\/10.1016\/s0140-6736(20)30414-1","journal-title":"Lancet"},{"key":"6662_CR13","doi-asserted-by":"publisher","first-page":"2009","DOI":"10.1056\/NEJMoa1903869","volume":"381","author":"S Biswal","year":"2019","unstructured":"Biswal S, Reynales H, Saez-Llorens X et al (2019) Efficacy of a Tetravalent Dengue Vaccine in Healthy Children and Adolescents. N Engl J Med 381:2009\u20132019. https:\/\/doi.org\/10.1056\/NEJMoa1903869","journal-title":"N Engl J Med"},{"key":"6662_CR14","doi-asserted-by":"publisher","first-page":"105516","DOI":"10.1016\/j.antiviral.2022.105516","volume":"210","author":"T Teramoto","year":"2023","unstructured":"Teramoto T, Choi KH, Padmanabhan R (2023) Flavivirus proteases: The viral Achilles heel to prevent future pandemics. Antiviral Res 210:105516. https:\/\/doi.org\/10.1016\/j.antiviral.2022.105516","journal-title":"Antiviral Res"},{"key":"6662_CR15","doi-asserted-by":"publisher","first-page":"S7","DOI":"10.1038\/nrmicro2460","volume":"8","author":"MG Guzman","year":"2010","unstructured":"Guzman MG, Halstead SB, Artsob H et al (2010) Dengue: a continuing global threat. Nat Rev Microbiol 8:S7\u201316. https:\/\/doi.org\/10.1038\/nrmicro2460","journal-title":"Nat Rev Microbiol"},{"key":"6662_CR16","doi-asserted-by":"publisher","first-page":"2467","DOI":"10.1128\/jvi.65.5.2467-2475.1991","volume":"65","author":"B Falgout","year":"1991","unstructured":"Falgout B, Pethel M, Zhang YM et al (1991) Both nonstructural proteins NS2B and NS3 are required for the proteolytic processing of dengue virus nonstructural proteins. J Virol 65:2467\u20132475. https:\/\/doi.org\/10.1128\/jvi.65.5.2467-2475.1991","journal-title":"J Virol"},{"key":"6662_CR17","doi-asserted-by":"publisher","first-page":"3108","DOI":"10.1128\/jvi.73.4.3108-3116.1999","volume":"73","author":"H Li","year":"1999","unstructured":"Li H, Clum S, You S et al (1999) The serine protease and RNA-stimulated nucleoside triphosphatase and RNA helicase functional domains of dengue virus type 2 NS3 converge within a region of 20 amino acids. J Virol 73:3108\u20133116. https:\/\/doi.org\/10.1128\/jvi.73.4.3108-3116.1999","journal-title":"J Virol"},{"key":"6662_CR18","doi-asserted-by":"publisher","first-page":"3889","DOI":"10.1093\/nar\/17.10.3889","volume":"17","author":"AE Gorbalenya","year":"1989","unstructured":"Gorbalenya AE, Donchenko AP, Koonin EV et al (1989) N-terminal domains of putative helicases of flavi- and pestiviruses may be serine proteases. Nucleic Acids Res 17:3889\u20133897. https:\/\/doi.org\/10.1093\/nar\/17.10.3889","journal-title":"Nucleic Acids Res"},{"key":"6662_CR19","doi-asserted-by":"publisher","first-page":"637","DOI":"10.1016\/0042-6822(89)90639-9","volume":"171","author":"JF Bazan","year":"1989","unstructured":"Bazan JF, Fletterick RJ (1989) Detection of a trypsin-like serine protease domain in flaviviruses and pestiviruses. Virology 171:637\u2013639. https:\/\/doi.org\/10.1016\/0042-6822(89)90639-9","journal-title":"Virology"},{"key":"6662_CR20","doi-asserted-by":"publisher","first-page":"107914","DOI":"10.1016\/j.bioorg.2024.107914","volume":"153","author":"EE Jo\u00e3o","year":"2024","unstructured":"Jo\u00e3o EE, Lopes JR, Guedes BFR et al (2024) Advances in drug discovery of flavivirus NS2B-NS3pro serine protease inhibitors for the treatment of Dengue, Zika, and West Nile viruses. Bioorg Chem 153:107914. https:\/\/doi.org\/10.1016\/j.bioorg.2024.107914","journal-title":"Bioorg Chem"},{"key":"6662_CR21","doi-asserted-by":"publisher","unstructured":"Starvaggi J, Previti S, Zappal\u00e0 M et al (2024) The Inhibition of NS2B\/NS3 Protease: A New Therapeutic Opportunity to Treat Dengue and Zika Virus Infection. Int J Mol Sci 25. https:\/\/doi.org\/10.3390\/ijms25084376","DOI":"10.3390\/ijms25084376"},{"key":"6662_CR22","doi-asserted-by":"publisher","first-page":"6832","DOI":"10.1021\/jacs.9b02505","volume":"141","author":"Y Yao","year":"2019","unstructured":"Yao Y, Huo T, Lin YL et al (2019) Discovery, X-ray Crystallography and Antiviral Activity of Allosteric Inhibitors of Flavivirus NS2B-NS3 Protease. J Am Chem Soc 141:6832\u20136836. https:\/\/doi.org\/10.1021\/jacs.9b02505","journal-title":"J Am Chem Soc"},{"key":"6662_CR23","doi-asserted-by":"publisher","first-page":"372","DOI":"10.1038\/nsmb1073","volume":"13","author":"P Erbel","year":"2006","unstructured":"Erbel P, Schiering N, D\u2019Arcy A et al (2006) Structural basis for the activation of flaviviral NS3 proteases from dengue and West Nile virus. Nat Struct Mol Biol 13:372\u2013373. https:\/\/doi.org\/10.1038\/nsmb1073","journal-title":"Nat Struct Mol Biol"},{"key":"6662_CR24","doi-asserted-by":"publisher","first-page":"1061937","DOI":"10.3389\/fcimb.2023.1061937","volume":"13","author":"H Norshidah","year":"2023","unstructured":"Norshidah H, Leow CH, Ezleen KE et al (2023) Assessing the potential of NS2B\/NS3 protease inhibitors biomarker in curbing dengue virus infections: In silico vs. In vitro approach. Front Cell Infect Microbiol 13:1061937. https:\/\/doi.org\/10.3389\/fcimb.2023.1061937","journal-title":"Front Cell Infect Microbiol"},{"key":"6662_CR25","doi-asserted-by":"publisher","first-page":"1355","DOI":"10.1039\/d0nh00207k","volume":"5","author":"D Chen","year":"2020","unstructured":"Chen D, Li J (2020) Ultrasmall Au nanoclusters for bioanalytical and biomedical applications: the undisclosed and neglected roles of ligands in determining the nanoclusters\u2019 catalytic activities. Nanoscale Horiz 5:1355\u20131367. https:\/\/doi.org\/10.1039\/d0nh00207k","journal-title":"Nanoscale Horiz"},{"key":"6662_CR26","doi-asserted-by":"publisher","first-page":"958626","DOI":"10.3389\/fchem.2022.958626","volume":"10","author":"D Cheng","year":"2022","unstructured":"Cheng D, Liu R, Hu K (2022) Gold nanoclusters: Photophysical properties and photocatalytic applications. Front Chem 10:958626. https:\/\/doi.org\/10.3389\/fchem.2022.958626","journal-title":"Front Chem"},{"key":"6662_CR27","doi-asserted-by":"publisher","first-page":"e2101032","DOI":"10.1002\/adhm.202101032","volume":"11","author":"W Ndugire","year":"2022","unstructured":"Ndugire W, Raviranga NGH, Lao J et al (2022) Gold Nanoclusters as Nanoantibiotic Auranofin Analogues. Adv Healthc Mater 11:e2101032. https:\/\/doi.org\/10.1002\/adhm.202101032","journal-title":"Adv Healthc Mater"},{"key":"6662_CR28","doi-asserted-by":"publisher","first-page":"110924","DOI":"10.1016\/j.msec.2020.110924","volume":"112","author":"L Chen","year":"2020","unstructured":"Chen L, Liang J (2020) An overview of functional nanoparticles as novel emerging antiviral therapeutic agents. Mater Sci Eng C Mater Biol Appl 112:110924. https:\/\/doi.org\/10.1016\/j.msec.2020.110924","journal-title":"Mater Sci Eng C Mater Biol Appl"},{"key":"6662_CR29","doi-asserted-by":"publisher","first-page":"969","DOI":"10.1021\/acsanm.7b00386","volume":"1","author":"Y Bai","year":"2018","unstructured":"Bai Y, Zhou Y, Liu H et al (2018) Glutathione-Stabilized Fluorescent Gold Nanoclusters Vary in Their Influences on the Proliferation of Pseudorabies Virus and Porcine Reproductive and Respiratory Syndrome Virus. ACS Appl Nano Mater 1:969\u2013976. https:\/\/doi.org\/10.1021\/acsanm.7b00386","journal-title":"ACS Appl Nano Mater"},{"key":"6662_CR30","doi-asserted-by":"publisher","first-page":"1800030","DOI":"10.1002\/gch2.201800030","volume":"2","author":"C Feng","year":"2018","unstructured":"Feng C, Fang P, Zhou Y et al (2018) Different Effects of His-Au NCs and MES-Au NCs on the Propagation of Pseudorabies Virus. Glob Chall 2:1800030. https:\/\/doi.org\/10.1002\/gch2.201800030","journal-title":"Glob Chall"},{"key":"6662_CR31","doi-asserted-by":"publisher","first-page":"101468","DOI":"10.1016\/j.nantod.2022.101468","volume":"44","author":"Z He","year":"2022","unstructured":"He Z, Ye F, Zhang C et al (2022) A comparison of Remdesivir versus gold cluster in COVID-19 animal model: A better therapeutic outcome of gold cluster. Nano Today 44:101468. https:\/\/doi.org\/10.1016\/j.nantod.2022.101468","journal-title":"Nano Today"},{"key":"6662_CR32","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1107\/s0907444909047337","volume":"66","author":"W Kabsch","year":"2010","unstructured":"Kabsch W (2010) XDS. Acta Crystallogr D Biol Crystallogr 66:125\u2013132. https:\/\/doi.org\/10.1107\/s0907444909047337","journal-title":"Acta Crystallogr D Biol Crystallogr"},{"key":"6662_CR33","doi-asserted-by":"publisher","first-page":"658","DOI":"10.1107\/s0021889807021206","volume":"40","author":"AJ McCoy","year":"2007","unstructured":"McCoy AJ, Grosse-Kunstleve RW, Adams PD et al (2007) Phaser crystallographic software. J Appl Crystallogr 40:658\u2013674. https:\/\/doi.org\/10.1107\/s0021889807021206","journal-title":"J Appl Crystallogr"},{"key":"6662_CR34","doi-asserted-by":"publisher","first-page":"2126","DOI":"10.1107\/s0907444904019158","volume":"60","author":"P Emsley","year":"2004","unstructured":"Emsley P, Cowtan K (2004) Coot: model-building tools for molecular graphics. Acta Crystallogr D Biol Crystallogr 60:2126\u20132132. https:\/\/doi.org\/10.1107\/s0907444904019158","journal-title":"Acta Crystallogr D Biol Crystallogr"},{"key":"6662_CR35","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1107\/s0907444909052925","volume":"66","author":"PD Adams","year":"2010","unstructured":"Adams PD, Afonine PV, Bunk\u00f3czi G et al (2010) PHENIX: a comprehensive Python-based system for macromolecular structure solution. Acta Crystallogr D Biol Crystallogr 66:213\u2013221. https:\/\/doi.org\/10.1107\/s0907444909052925","journal-title":"Acta Crystallogr D Biol Crystallogr"},{"key":"6662_CR36","doi-asserted-by":"publisher","first-page":"355","DOI":"10.1107\/s0907444911001314","volume":"67","author":"GN Murshudov","year":"2011","unstructured":"Murshudov GN, Skub\u00e1k P, Lebedev AA et al (2011) REFMAC5 for the refinement of macromolecular crystal structures. Acta Crystallogr D Biol Crystallogr 67:355\u2013367. https:\/\/doi.org\/10.1107\/s0907444911001314","journal-title":"Acta Crystallogr D Biol Crystallogr"},{"key":"6662_CR37","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1107\/s0907444909042073","volume":"66","author":"VB Chen","year":"2010","unstructured":"Chen VB, Arendall WB 3rd, Headd JJ et al (2010) MolProbity: all-atom structure validation for macromolecular crystallography. Acta Crystallogr D Biol Crystallogr 66:12\u201321. https:\/\/doi.org\/10.1107\/s0907444909042073","journal-title":"Acta Crystallogr D Biol Crystallogr"},{"key":"6662_CR38","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1007\/978-1-4939-6887-9_22","volume":"1586","author":"S Gr\u00e4slund","year":"2017","unstructured":"Gr\u00e4slund S, Savitsky P, M\u00fcller-Knapp S (2017) In Vivo Biotinylation of Antigens in E. coli. Methods Mol Biol 1586:337\u2013344. https:\/\/doi.org\/10.1007\/978-1-4939-6887-9_22","journal-title":"Methods Mol Biol"},{"key":"6662_CR39","doi-asserted-by":"publisher","first-page":"2744","DOI":"10.1021\/cb400612h","volume":"8","author":"M Yildiz","year":"2013","unstructured":"Yildiz M, Ghosh S, Bell JA et al (2013) Allosteric inhibition of the NS2B-NS3 protease from dengue virus. ACS Chem Biol 8:2744\u20132752. https:\/\/doi.org\/10.1021\/cb400612h","journal-title":"ACS Chem Biol"},{"key":"6662_CR40","unstructured":"Team RC (2025) R: A Language and Environment for Statistical Computing R Foundation for Statistical Computing. Vienna, Austria. https:\/\/www.R-project.org\/"},{"key":"6662_CR41","doi-asserted-by":"publisher","first-page":"13951","DOI":"10.1021\/acs.jpcc.9b01184","volume":"123","author":"K Wang","year":"2019","unstructured":"Wang K, Su W, Gao F et al (2019) Glutathione-Coated Au29(SG)27: Structural Determination Based on Different Combination Styles Confirmed by Experiments. J Phys Chem C 123:13951\u201313957. https:\/\/doi.org\/10.1021\/acs.jpcc.9b01184","journal-title":"J Phys Chem C"},{"key":"6662_CR42","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1016\/0263-7855(96)00018-5","volume":"14","author":"W Humphrey","year":"1996","unstructured":"Humphrey W, Dalke A, Schulten K (1996) VMD: visual molecular dynamics. J Mol Graph 14:33\u201338. https:\/\/doi.org\/10.1016\/0263-7855(96)00018-5","journal-title":"J Mol Graph"},{"key":"6662_CR43","doi-asserted-by":"publisher","first-page":"405","DOI":"10.1021\/acs.jctc.5b00935","volume":"12","author":"J Lee","year":"2016","unstructured":"Lee J, Cheng X, Swails JM et al (2016) CHARMM-GUI Input Generator for NAMD, GROMACS, AMBER, OpenMM, and CHARMM\/OpenMM Simulations Using the CHARMM36 Additive Force Field. J Chem Theory Comput 12:405\u2013413. https:\/\/doi.org\/10.1021\/acs.jctc.5b00935","journal-title":"J Chem Theory Comput"},{"key":"6662_CR44","doi-asserted-by":"publisher","first-page":"1781","DOI":"10.1002\/jcc.20289","volume":"26","author":"JC Phillips","year":"2005","unstructured":"Phillips JC, Braun R, Wang W et al (2005) Scalable molecular dynamics with NAMD. J Comput Chem 26:1781\u20131802. https:\/\/doi.org\/10.1002\/jcc.20289","journal-title":"J Comput Chem"},{"key":"6662_CR45","doi-asserted-by":"publisher","first-page":"e36872","DOI":"10.1371\/journal.pone.0036872","volume":"7","author":"M Junaid","year":"2012","unstructured":"Junaid M, Chalayut C, Sehgelmeble Torrejon A et al (2012) Enzymatic analysis of recombinant Japanese encephalitis virus NS2B(H)-NS3pro protease with fluorogenic model peptide substrates. PLoS ONE 7:e36872. https:\/\/doi.org\/10.1371\/journal.pone.0036872","journal-title":"PLoS ONE"},{"key":"6662_CR46","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1186\/s41182-016-0025-6","volume":"44","author":"GE Ulanday","year":"2016","unstructured":"Ulanday GE, Okamoto K, Morita K (2016) Development and utility of an in vitro, fluorescence-based assay for the discovery of novel compounds against dengue 2 viral protease. Trop Med Health 44:22. https:\/\/doi.org\/10.1186\/s41182-016-0025-6","journal-title":"Trop Med Health"},{"key":"6662_CR47","doi-asserted-by":"publisher","first-page":"607","DOI":"10.1186\/s12859-021-04519-4","volume":"22","author":"J Guerra","year":"2021","unstructured":"Guerra J, Ribeiro-Filho HV, Jara GE et al (2021) pyKVFinder: an efficient and integrable Python package for biomolecular cavity detection and characterization in data science. BMC Bioinformatics 22:607. https:\/\/doi.org\/10.1186\/s12859-021-04519-4","journal-title":"BMC Bioinformatics"},{"key":"6662_CR48","doi-asserted-by":"publisher","first-page":"3059","DOI":"10.1128\/jvi.02044-09","volume":"84","author":"S Chandramouli","year":"2010","unstructured":"Chandramouli S, Joseph JS, Daudenarde S et al (2010) Serotype-specific structural differences in the protease-cofactor complexes of the dengue virus family. J Virol 84:3059\u20133067. https:\/\/doi.org\/10.1128\/jvi.02044-09","journal-title":"J Virol"},{"key":"6662_CR49","doi-asserted-by":"publisher","first-page":"438","DOI":"10.1128\/jvi.06225-11","volume":"86","author":"CG Noble","year":"2012","unstructured":"Noble CG, Seh CC, Chao AT et al (2012) Ligand-bound structures of the dengue virus protease reveal the active conformation. J Virol 86:438\u2013446. https:\/\/doi.org\/10.1128\/jvi.06225-11","journal-title":"J Virol"},{"key":"6662_CR50","doi-asserted-by":"publisher","unstructured":"Quek JP, Ser Z, Chew BLA et al (2022) Dynamic Interactions of Post Cleaved NS2B Cofactor and NS3 Protease Identified by Integrative Structural Approaches. Viruses 14https:\/\/doi.org\/10.3390\/v14071440","DOI":"10.3390\/v14071440"},{"key":"6662_CR51","doi-asserted-by":"publisher","first-page":"129521","DOI":"10.1016\/j.bbagen.2020.129521","volume":"1864","author":"GD Noske","year":"2020","unstructured":"Noske GD, Gawriljuk VO, Fernandes RS et al (2020) Structural characterization and polymorphism analysis of the NS2B-NS3 protease from the 2017 Brazilian circulating strain of Yellow Fever virus. Biochim Biophys Acta Gen Subj 1864:129521. https:\/\/doi.org\/10.1016\/j.bbagen.2020.129521","journal-title":"Biochim Biophys Acta Gen Subj"},{"key":"6662_CR52","doi-asserted-by":"publisher","first-page":"13410","DOI":"10.1038\/ncomms13410","volume":"7","author":"WW Phoo","year":"2016","unstructured":"Phoo WW, Li Y, Zhang Z et al (2016) Structure of the NS2B-NS3 protease from Zika virus after self-cleavage. Nat Commun 7:13410. https:\/\/doi.org\/10.1038\/ncomms13410","journal-title":"Nat Commun"},{"key":"6662_CR53","doi-asserted-by":"publisher","first-page":"503","DOI":"10.1126\/science.aag2419","volume":"353","author":"J Lei","year":"2016","unstructured":"Lei J, Hansen G, Nitsche C et al (2016) Crystal structure of Zika virus NS2B-NS3 protease in complex with a boronate inhibitor. Science 353:503\u2013505. https:\/\/doi.org\/10.1126\/science.aag2419","journal-title":"Science"},{"key":"6662_CR54","doi-asserted-by":"publisher","first-page":"110451","DOI":"10.1016\/j.jbc.2025.110451","volume":"301","author":"L Corliss","year":"2025","unstructured":"Corliss L, Petit CM, Lennemann NJ (2025) Subcellular determinants of orthoflavivirus protease activity. J Biol Chem 301:110451. https:\/\/doi.org\/10.1016\/j.jbc.2025.110451","journal-title":"J Biol Chem"},{"key":"6662_CR55","doi-asserted-by":"publisher","first-page":"e0007202","DOI":"10.1371\/journal.pntd.0007202","volume":"13","author":"C Zou","year":"2019","unstructured":"Zou C, Huang C, Zhang J et al (2019) Virulence difference of five type I dengue viruses and the intrinsic molecular mechanism. PLoS Negl Trop Dis 13:e0007202. https:\/\/doi.org\/10.1371\/journal.pntd.0007202","journal-title":"PLoS Negl Trop Dis"},{"key":"6662_CR56","doi-asserted-by":"publisher","first-page":"18022","DOI":"10.1021\/jacs.0c06793","volume":"142","author":"D Budhadev","year":"2020","unstructured":"Budhadev D, Poole E, Nehlmeier I et al (2020) Glycan-Gold Nanoparticles as Multifunctional Probes for Multivalent Lectin-Carbohydrate Binding: Implications for Blocking Virus Infection and Nanoparticle Assembly. J Am Chem Soc 142:18022\u201318034. https:\/\/doi.org\/10.1021\/jacs.0c06793","journal-title":"J Am Chem Soc"},{"key":"6662_CR57","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1038\/nmat5053","volume":"17","author":"V Cagno","year":"2018","unstructured":"Cagno V, Andreozzi P, D\u2019Alicarnasso M et al (2018) Broad-spectrum non-toxic antiviral nanoparticles with a virucidal inhibition mechanism. Nat Mater 17:195\u2013203. https:\/\/doi.org\/10.1038\/nmat5053","journal-title":"Nat Mater"},{"key":"6662_CR58","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1186\/s12985-017-0761-1","volume":"14","author":"LL Garc\u00eda","year":"2017","unstructured":"Garc\u00eda LL, Padilla L, Casta\u00f1o JC (2017) Inhibitors compounds of the flavivirus replication process. Virol J 14:95. https:\/\/doi.org\/10.1186\/s12985-017-0761-1","journal-title":"Virol J"}],"container-title":["Archives of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00705-026-06662-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00705-026-06662-w","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00705-026-06662-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T07:02:04Z","timestamp":1785135724000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00705-026-06662-w"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,23]]},"references-count":58,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["6662"],"URL":"https:\/\/doi.org\/10.1007\/s00705-026-06662-w","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-8498727\/v1","asserted-by":"object"}]},"ISSN":["0304-8608","1432-8798"],"issn-type":[{"value":"0304-8608","type":"print"},{"value":"1432-8798","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,23]]},"assertion":[{"value":"2 January 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 May 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 June 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no known competing financial interests or personal relationships that could have influenced the work reported in this paper.","order":1,"name":"Ethics","label":"Declaration of competing interest","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"219"}}