{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T04:22:04Z","timestamp":1787458924670,"version":"build-2736575974"},"reference-count":14,"publisher":"Springer Science and Business Media LLC","issue":"8","license":[{"start":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T00:00:00Z","timestamp":1784764800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T00:00:00Z","timestamp":1784764800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Arch Virol"],"published-print":{"date-parts":[[2026,8]]},"DOI":"10.1007\/s00705-026-06696-0","type":"journal-article","created":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T03:50:58Z","timestamp":1784778658000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Reassessment of PRRSV-2 ORF5 sequence diversity in Japan identifies a previously unrecognized candidate lineage-level clade"],"prefix":"10.1007","volume":"171","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-2328-6682","authenticated-orcid":false,"given":"Osamu","family":"Taira","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryosuke","family":"Takai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoriko","family":"Yonezawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuyuki","family":"Tsutsumi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsuaki","family":"Sugiura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,7,23]]},"reference":[{"key":"6696_CR1","doi-asserted-by":"publisher","first-page":"106627","DOI":"10.1016\/j.prevetmed.2025.106627","volume":"244","author":"O Osemeke","year":"2025","unstructured":"Osemeke O, Silva GS, Corzo CA et al (2025) Economic impact of productivity losses attributable to porcine reproductive and respiratory syndrome virus in United States pork production, 2016\u20132020. Prev Vet Med 244:106627. https:\/\/doi.org\/10.1016\/j.prevetmed.2025.106627","journal-title":"Prev Vet Med"},{"key":"6696_CR2","doi-asserted-by":"publisher","first-page":"8700","DOI":"10.1128\/jvi.02551-09","volume":"84","author":"M Shi","year":"2010","unstructured":"Shi M, Lam TT-Y, Hon C-C et al (2010) Phylogeny-Based Evolutionary, Demographical, and Geographical Dissection of North American Type 2 Porcine Reproductive and Respiratory Syndrome Viruses. J Virol 84:8700\u20138711. https:\/\/doi.org\/10.1128\/jvi.02551-09","journal-title":"J Virol"},{"key":"6696_CR3","doi-asserted-by":"publisher","first-page":"e02916","DOI":"10.1128\/spectrum.02916-23","volume":"11","author":"W Yim-im","year":"2023","unstructured":"Yim-im W, Anderson TK, Paploski IAD et al (2023) Refining PRRSV-2 genetic classification based on global ORF5 sequences and investigation of their geographic distributions and temporal changes. Microbiol Spectr 11:e02916\u2013e02923. https:\/\/doi.org\/10.1128\/spectrum.02916-23","journal-title":"Microbiol Spectr"},{"key":"6696_CR4","doi-asserted-by":"publisher","unstructured":"Takai R, Sato T, Yonezawa Y et al (2025) Spatiotemporal distribution and genetic characteristics of porcine reproductive and respiratory syndrome virus-2 lineage 1 in Japan from 2020 to 2023. Res Vet Sci 106025. https:\/\/doi.org\/10.1016\/j.rvsc.2025.106025","DOI":"10.1016\/j.rvsc.2025.106025"},{"key":"6696_CR5","doi-asserted-by":"publisher","first-page":"608","DOI":"10.3390\/vaccines9060608","volume":"9","author":"IAD Paploski","year":"2021","unstructured":"Paploski IAD, Pamornchainavakul N, Makau DN et al (2021) Phylogenetic Structure and Sequential Dominance of Sub-Lineages of PRRSV Type-2 Lineage 1 in the United States. Vaccines 9:608. https:\/\/doi.org\/10.3390\/vaccines9060608","journal-title":"Vaccines"},{"key":"6696_CR6","doi-asserted-by":"publisher","first-page":"2486","DOI":"10.3389\/fmicb.2019.02486","volume":"10","author":"IAD Paploski","year":"2019","unstructured":"Paploski IAD, Corzo C, Rovira A et al (2019) Temporal Dynamics of Co-circulating Lineages of Porcine Reproductive and Respiratory Syndrome Virus. Front Microbiol 10:2486. https:\/\/doi.org\/10.3389\/fmicb.2019.02486","journal-title":"Front Microbiol"},{"key":"6696_CR7","doi-asserted-by":"publisher","first-page":"1419340","DOI":"10.3389\/fvets.2024.1419340","volume":"11","author":"MA Zeller","year":"2024","unstructured":"Zeller MA, Chang J, Trevisan G et al (2024) Rapid PRRSV-2 ORF5-based lineage classification using Nextclade. Front Vet Sci 11:1419340. https:\/\/doi.org\/10.3389\/fvets.2024.1419340","journal-title":"Front Vet Sci"},{"key":"6696_CR8","doi-asserted-by":"publisher","first-page":"msae263","DOI":"10.1093\/molbev\/msae263","volume":"41","author":"S Kumar","year":"2024","unstructured":"Kumar S, Stecher G, Suleski M et al (2024) MEGA12: Molecular Evolutionary Genetic Analysis Version 12 for Adaptive and Green Computing. Mol Biol Evol 41:msae263. https:\/\/doi.org\/10.1093\/molbev\/msae263","journal-title":"Mol Biol Evol"},{"key":"6696_CR9","doi-asserted-by":"publisher","first-page":"1792","DOI":"10.1093\/nar\/gkh340","volume":"32","author":"RC Edgar","year":"2004","unstructured":"Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32:1792\u20131797. https:\/\/doi.org\/10.1093\/nar\/gkh340","journal-title":"Nucleic Acids Res"},{"key":"6696_CR10","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1109\/mcse.2007.55","volume":"9","author":"JD Hunter","year":"2007","unstructured":"Hunter JD (2007) Matplotlib: A 2D Graphics Environment. Comput Sci Eng 9:90\u201395. https:\/\/doi.org\/10.1109\/mcse.2007.55","journal-title":"Comput Sci Eng"},{"key":"6696_CR11","doi-asserted-by":"publisher","first-page":"1433931","DOI":"10.3389\/fviro.2024.1433931","volume":"4","author":"K VanderWaal","year":"2024","unstructured":"VanderWaal K, Pamornchainavakul N, Kikuti M et al (2024) Phylogenetic-based methods for fine-scale classification of PRRSV-2 ORF5 sequences: a comparison of their robustness and reproducibility. Front Virol 4:1433931. https:\/\/doi.org\/10.3389\/fviro.2024.1433931","journal-title":"Front Virol"},{"key":"6696_CR12","doi-asserted-by":"publisher","first-page":"e00709","DOI":"10.1128\/msphere.00709-24","volume":"10","author":"K VanderWaal","year":"2025","unstructured":"VanderWaal K, Pamornchainavakul N, Kikuti M et al (2025) PRRSV-2 variant classification: a dynamic nomenclature for enhanced monitoring and surveillance. mSphere 10:e00709\u2013e00724. https:\/\/doi.org\/10.1128\/msphere.00709-24","journal-title":"mSphere"},{"key":"6696_CR13","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1186\/s40813-025-00470-5","volume":"11","author":"S-C Kim","year":"2025","unstructured":"Kim S-C, Kang SC, Kim H-J et al (2025) Nationwide emergence and spread of highly virulent PRRSV-2 mutants in Korea. Porc Heal Manag 11:58. https:\/\/doi.org\/10.1186\/s40813-025-00470-5","journal-title":"Porc Heal Manag"},{"key":"6696_CR14","doi-asserted-by":"publisher","first-page":"346","DOI":"10.3390\/pathogens15040346","volume":"15","author":"JPH da Silva","year":"2026","unstructured":"da Silva JPH, Paploski IAD, Charette R et al (2026) Phylogenetic Lineages of PRRSV-2 from Canada Reveal Patterns of Transboundary Spread and Two Novel Sub-Lineages in North America. Pathogens 15:346. https:\/\/doi.org\/10.3390\/pathogens15040346","journal-title":"Pathogens"}],"container-title":["Archives of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00705-026-06696-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00705-026-06696-0","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00705-026-06696-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T04:01:41Z","timestamp":1787457701000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00705-026-06696-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,23]]},"references-count":14,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["6696"],"URL":"https:\/\/doi.org\/10.1007\/s00705-026-06696-0","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-9175809\/v1","asserted-by":"object"}]},"ISSN":["0304-8608","1432-8798"],"issn-type":[{"value":"0304-8608","type":"print"},{"value":"1432-8798","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,23]]},"assertion":[{"value":"19 March 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 July 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 July 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","label":"Competing interests","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"232"}}