{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T09:23:04Z","timestamp":1768468984817,"version":"3.49.0"},"reference-count":53,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2020,5,1]],"date-time":"2020-05-01T00:00:00Z","timestamp":1588291200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2020,5,1]],"date-time":"2020-05-01T00:00:00Z","timestamp":1588291200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"funder":[{"name":"Science and Technology Open Cooperation Project of Henan Province","award":["182106000035"],"award-info":[{"award-number":["182106000035"]}]},{"name":"Earmarked Fund for Beijing Dairy Industry Innovation Consortium of Agriculture Research System","award":["BAIC06-2019"],"award-info":[{"award-number":["BAIC06-2019"]}]},{"name":"Chinese Academy of Agricultural Science and Technology Innovation Project","award":["CAAS-XTCX2016011-01"],"award-info":[{"award-number":["CAAS-XTCX2016011-01"]}]},{"DOI":"10.13039\/501100012421","name":"Agricultural Science and Technology Innovation Program","doi-asserted-by":"crossref","award":["CAAS-ASTIP-2017-FRI-04"],"award-info":[{"award-number":["CAAS-ASTIP-2017-FRI-04"]}],"id":[{"id":"10.13039\/501100012421","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Fundamental Research Funds for Central Non-profit Scientific Institution","award":["CAAS-ASTIP-2017-FRI-04"],"award-info":[{"award-number":["CAAS-ASTIP-2017-FRI-04"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Amino Acids"],"published-print":{"date-parts":[[2020,5]]},"DOI":"10.1007\/s00726-020-02852-1","type":"journal-article","created":{"date-parts":[[2020,5,5]],"date-time":"2020-05-05T20:03:31Z","timestamp":1588709011000},"page":"781-792","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Integrating RNA-sequencing and untargeted LC\u2013MS metabolomics to evaluate the effect of lysine deficiency on hepatic functions in Holstein calves"],"prefix":"10.1007","volume":"52","author":[{"given":"Fanlin","family":"Kong","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanliang","family":"Bi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bing","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kai","family":"Cui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tong","family":"Fu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiyu","family":"Diao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Tu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,5,5]]},"reference":[{"key":"2852_CR1","doi-asserted-by":"publisher","first-page":"628","DOI":"10.2527\/1998.762628x","volume":"76","author":"M Abe","year":"1998","unstructured":"Abe M, Iriki T, Funaba M, Onda S (1998) Limiting amino acids for a corn and soybean meal diet in weaned calves less than three months of age. J Anim Sci 76:628\u2013636","journal-title":"J Anim Sci"},{"key":"2852_CR2","doi-asserted-by":"publisher","first-page":"651","DOI":"10.1080\/00071668.2012.726349","volume":"53","author":"MR Alam","year":"2012","unstructured":"Alam MR, Yoshizawa F, Sugahara K (2012) Oral administration of lysine restores food intake and ventromedial hypothalamic dopamine in chicken on a lysine-free diet. British Poult Sci 53:651\u2013657","journal-title":"British Poult Sci"},{"key":"2852_CR3","first-page":"589","volume":"14","author":"M Ameen","year":"1987","unstructured":"Ameen M, Palmer T, Oberholzer VG (1987) Inhibition of bovine liver lysine-ketoglutarate reductase by urea cycle metabolites and saccharine. Biochem Int 14:589\u2013595","journal-title":"Biochem Int"},{"key":"2852_CR4","doi-asserted-by":"publisher","first-page":"9717","DOI":"10.1523\/JNEUROSCI.0381-15.2015","volume":"35","author":"V Anja","year":"2015","unstructured":"Anja V, Juliane B, Masataka N, Sandra H, Ulrich B, Wolfgang M (2015) Transsynaptic tracing from taste receptor cells reveals local taste receptor gene expression in gustatory ganglia and brain. J Neurosci 35:9717\u20139729","journal-title":"J Neurosci"},{"key":"2852_CR5","volume-title":"Official methods of analysis of the association of offical analytical chemists","author":"Association of Official Analytical Chemists","year":"2012","unstructured":"Association of Official Analytical Chemists (2012) Official methods of analysis of the association of offical analytical chemists. AOAC International, Gaithersburg"},{"key":"2852_CR6","doi-asserted-by":"publisher","first-page":"680","DOI":"10.1016\/j.tibs.2006.10.002","volume":"31","author":"JL Bos","year":"2006","unstructured":"Bos JL (2006) Epac proteins: multi-purpose cAMP targets. Trends Biochem Sci 31:680\u2013686","journal-title":"Trends Biochem Sci"},{"key":"2852_CR7","doi-asserted-by":"publisher","first-page":"1801","DOI":"10.2527\/jas.2012-5260","volume":"91","author":"R Carrasco","year":"2013","unstructured":"Carrasco R, Arrizon AA, Plascencia A, Torrentera NG, Zinn RA (2013) Comparative feeding value of distillers dried grains plus solubles as a partial replacement for steam-flaked corn in diets for calf-fed Holstein steers: characteristics of digestion, growth performance, and dietary energetics. J Anim Sci 91:1801\u20131810","journal-title":"J Anim Sci"},{"key":"2852_CR8","first-page":"423","volume":"19","author":"T Ci\u0307Velek","year":"2013","unstructured":"Ci\u0307Velek T, Bi\u0307Rdane F, Kabu M, Cingi CC, Acar A (2013) Effects of methionine and lysine on metabolic profile in dairy cattle during periparturient period. Kafkas Univ Vet Fak 19:423\u2013432","journal-title":"Kafkas Univ Vet Fak"},{"key":"2852_CR9","doi-asserted-by":"publisher","first-page":"3851","DOI":"10.3168\/jds.S0022-0302(05)73070-8","volume":"88","author":"HM Dann","year":"2005","unstructured":"Dann HM, Drackley JK (2005) Carnitine palmitoyltransferase I in liver of periparturient dairy cows: effects of prepartum intake, postpartum induction of ketosis, and periparturient disorders. J Dairy Sci 88:3851\u20133859","journal-title":"J Dairy Sci"},{"key":"2852_CR10","doi-asserted-by":"publisher","first-page":"681","DOI":"10.3168\/jds.S0022-0302(85)80874-2","volume":"68","author":"PB Donahue","year":"1985","unstructured":"Donahue PB, Schwab CG, Quigley JD, Hylton WE (1985) Methionine deficiency in early-weaned dairy calves fed pelleted rations based on corn and alfalfa or corn and soybean proteins. J Dairy Sci 68:681\u2013693","journal-title":"J Dairy Sci"},{"key":"2852_CR11","doi-asserted-by":"crossref","first-page":"10764","DOI":"10.1016\/S0021-9258(18)90577-6","volume":"259","author":"WA Dunn","year":"1984","unstructured":"Dunn WA, Rettura G, Seifter E, Englard S (1984) Carnitine biosynthesis from gamma-butyrobetaine and from exogenous protein-bound 6-N-trimethyl-l-lysine by the perfused guinea pig liver. Effect of ascorbate deficiency on the in situ activity of gamma-butyrobetaine hydroxylase. J Biol Chem 259:10764\u201310770","journal-title":"J Biol Chem"},{"key":"2852_CR12","first-page":"213","volume":"21","author":"J Flaga","year":"2018","unstructured":"Flaga J, Korytkowski \u00c5, G\u00e3Rka P, Kowalski ZM (2018) Age-related changes in mRNA expression of selected surface receptors in lymphocytes of dairy calves. Pol J Vet Sci 21:213\u2013216","journal-title":"Pol J Vet Sci"},{"key":"2852_CR13","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1007\/s00424-012-1126-7","volume":"465","author":"UG Friis","year":"2013","unstructured":"Friis UG, Madsen K, Stubbe J, Hansen PB, Svenningsen P, Bie P, Skott O, Jensen B (2013) Regulation of renin secretion by renal juxtaglomerular cells. Pfl\u00fcgers Arch Eur J Physiol 465:25\u201337","journal-title":"Pfl\u00fcgers Arch Eur J Physiol"},{"key":"2852_CR14","doi-asserted-by":"publisher","first-page":"9888","DOI":"10.1073\/pnas.0702448104","volume":"104","author":"CB Green","year":"2007","unstructured":"Green CB, Douris N, Kojima S, Strayer CA, Fogerty J, Lourim D, Keller SR, Besharse JC (2007) Loss of Nocturnin, a circadian deadenylase, confers resistance to hepatic steatosis and diet-induced obesity. Proc Natl Acad Sci USA 104:9888\u20139893","journal-title":"Proc Natl Acad Sci USA"},{"key":"2852_CR15","doi-asserted-by":"publisher","first-page":"3882","DOI":"10.3168\/jds.S0022-0302(93)77729-2","volume":"76","author":"RR Grummer","year":"1993","unstructured":"Grummer RR (1993) Etiology of lipid-related metabolic disorders in periparturient dairy cows. J Dairy Sci 76:3882\u20133896","journal-title":"J Dairy Sci"},{"key":"2852_CR16","first-page":"1","volume":"3","author":"ML Halls","year":"2011","unstructured":"Halls ML, Cooper DMF (2011) Regulation by Ca2+-signaling pathways of adenylyl cyclases. CSH Perspect Biol 3:1\u201322","journal-title":"CSH Perspect Biol"},{"key":"2852_CR17","doi-asserted-by":"publisher","first-page":"1558","DOI":"10.1038\/oby.2012.37","volume":"20","author":"SW Hee","year":"2012","unstructured":"Hee SW, Tsai SH, Chang YC, Chang CJ, Yu IS, Lee PC, Lee WJ, Chang EYC, Chuang LM (2012) The Role of Nocturnin in Early Adipogenesis and Modulation of Systemic Insulin Resistance in Human. Obesity 20:1558\u20131565","journal-title":"Obesity"},{"key":"2852_CR18","doi-asserted-by":"publisher","first-page":"2347","DOI":"10.1271\/bbb.120589","volume":"76","author":"K Hiroyuki","year":"2012","unstructured":"Hiroyuki K, Aiko I, Akane A, Kazuki N, Masaya K (2012) Effects of dietary low level of threonine and lysine on the accumulation of intramuscular fat in porcine muscle. Biosci Biotechnol Biochem 76:2347\u20132350","journal-title":"Biosci Biotechnol Biochem"},{"key":"2852_CR19","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1016\/j.livsci.2012.06.025","volume":"149","author":"M Katsumata","year":"2012","unstructured":"Katsumata M, Kyoya T, Ishida A, Ohtsuka M, Nakashima K (2012) Dose-dependent response of intramuscular fat accumulation in longissimus dorsi muscle of finishing pigs to dietary lysine levels. Livest Sci 149:41\u201345","journal-title":"Livest Sci"},{"key":"2852_CR20","doi-asserted-by":"publisher","first-page":"427","DOI":"10.1111\/asj.12150","volume":"85","author":"M Keisuke","year":"2014","unstructured":"Keisuke M, Fumika Y, Masanari T, Masakazu I (2014) Effects of dietary lysine\/protein ratio and fat levels on growth performance and meat quality of finishing pigs. Anim Sci J 85:427\u2013434","journal-title":"Anim Sci J"},{"key":"2852_CR21","doi-asserted-by":"publisher","first-page":"244","DOI":"10.1186\/s12864-016-2578-5","volume":"17","author":"K Keogh","year":"2016","unstructured":"Keogh K, Kenny DA, Cormican P, Kelly AK, Waters SM (2016) Effect of dietary restriction and subsequent re-alimentation on the transcriptional profile of hepatic tissue in cattle. BMC Genom 17:244","journal-title":"BMC Genom"},{"key":"2852_CR22","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1111\/jpn.12061","volume":"98","author":"J Kim","year":"2014","unstructured":"Kim J, Lee KS, Kwon DH, Bong JJ, Jeong JY, Nam YS, Lee MS, Liu X, Biak M (2014) Severe dietary lysine restriction affects growth and body composition and hepatic gene expression for nitrogen metabolism in growing rats. J Anim Physiol Anim Nutr 98:149\u2013157","journal-title":"J Anim Physiol Anim Nutr"},{"key":"2852_CR23","doi-asserted-by":"publisher","first-page":"87","DOI":"10.11118\/actaun201058010087","volume":"58","author":"L K\u0159\u00ed\u017eov\u00e1","year":"2010","unstructured":"K\u0159\u00ed\u017eov\u00e1 L, T\u0159in\u00e1ct\u00fd J, Svobodov\u00e1 J, Richter M, \u010cern\u00fd V, Jaro\u0161ov\u00e1 A (2010) Effect of supplemental rumenprotected lysine, methionine or both added to diet of lactating dairy cows on milk fatty acids profile. Acta Univ Agric Silvic Mendelianae Brun 58:87\u201394","journal-title":"Acta Univ Agric Silvic Mendelianae Brun"},{"key":"2852_CR24","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1080\/87559129809541162","volume":"14","author":"T Kunio","year":"1998","unstructured":"Kunio T (1998) Central mechanism of umami taste perception and effect of dietary protein on the preference for amino acids and sodium chloride in rats. Food Rev Int 14:273\u2013308","journal-title":"Food Rev Int"},{"key":"2852_CR25","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1042\/bj1600085","volume":"160","author":"J Labadie","year":"1976","unstructured":"Labadie J, Dunn WA, Aronson NN (1976) Hepatic synthesis of carnitine from protein-bound trimethyl-lysine. Lysosomal digestion of methyl-lysine-labelled asialo-fetuin. Biochem J 160:85\u201395","journal-title":"Biochem J"},{"key":"2852_CR26","doi-asserted-by":"publisher","first-page":"360","DOI":"10.1017\/S1751731108003571","volume":"3","author":"H Lapierre","year":"2009","unstructured":"Lapierre H, Doepel L, Milne E, Lobley GE (2009) Responses in mammary and splanchnic metabolism to altered lysine supply in dairy cows. Animal 3:360\u2013371","journal-title":"Animal"},{"key":"2852_CR27","doi-asserted-by":"publisher","first-page":"e84589","DOI":"10.1371\/journal.pone.0084589","volume":"9","author":"S Luigi","year":"2014","unstructured":"Luigi S, Alfonso G, Domenico C, Domenico C, Luisa BM (2014) Where does N\u03b5-trimethyllysine for the carnitine biosynthesis in mammals come from? PLoS ONE 9:e84589","journal-title":"PLoS ONE"},{"key":"2852_CR28","doi-asserted-by":"publisher","first-page":"3177","DOI":"10.2527\/jas.2012-5424","volume":"91","author":"MS Madeira","year":"2013","unstructured":"Madeira MS, Costa P, Alfaia CM, Lopes PA, Bessa RJB, Lemos JPC, Prates JAM (2013) The increased intramuscular fat promoted by dietary lysine restriction in lean but not in fatty pig genotypes improves pork sensory attributes. J Anim Sci 91:3177\u20133187","journal-title":"J Anim Sci"},{"key":"2852_CR29","doi-asserted-by":"crossref","first-page":"11643","DOI":"10.1016\/S0021-9258(20)71252-4","volume":"259","author":"PJ Markovitz","year":"1984","unstructured":"Markovitz PJ, Chuang DT, Cox RP (1984) Familial hyperlysinemias. Purification and characterization of the bifunctional aminoadipic semialdehyde synthase with lysine-ketoglutarate reductase and saccharopine dehydrogenase activities. J Biol Chem 259:11643\u201311646","journal-title":"J Biol Chem"},{"key":"2852_CR30","volume-title":"Nutrient requirements of dairy cattle","author":"National Research Council","year":"2001","unstructured":"National Research Council (2001) Nutrient requirements of dairy cattle. National Academy Press, Washington"},{"key":"2852_CR31","doi-asserted-by":"publisher","first-page":"28","DOI":"10.3390\/ani9010028","volume":"9","author":"I Ogunade","year":"2019","unstructured":"Ogunade I, Schweickart H, Mccoun M, Cannon K, Mcmanus C (2019) Integrating 16S rRNA sequencing and LC\u2013MS-based metabolomics to evaluate the effects of live yeast on rumen function in beef cattle. Animals 9:28","journal-title":"Animals"},{"key":"2852_CR32","doi-asserted-by":"publisher","first-page":"544","DOI":"10.1016\/j.nutres.2011.06.001","volume":"31","author":"DBS Pink","year":"2011","unstructured":"Pink DBS, Gatrell SK, Elango R, Turchinsky J, Kiess AS, Blemings KP, Dixon WT, Ball RO (2011) Lysine \u03b1-ketoglutarate reductase, but not saccharopine dehydrogenase, is subject to substrate inhibition in pig liver. Nutrit Res 31:544\u2013554","journal-title":"Nutrit Res"},{"key":"2852_CR33","doi-asserted-by":"publisher","first-page":"1144","DOI":"10.1096\/fj.15-277798","volume":"30","author":"CK Pon","year":"2016","unstructured":"Pon CK, Lane JR, Sloan EK, Halls ML (2016) The \u03b22-adrenoceptor activates a positive cAMP-calcium feedforward loop to drive breast cancer cell invasion. The FASEB J 30:1144\u20131154","journal-title":"The FASEB J"},{"key":"2852_CR34","doi-asserted-by":"publisher","first-page":"3651","DOI":"10.2527\/jas.2010-3791","volume":"89","author":"JA Rodr\u00edguez-S\u00e1nchez","year":"2011","unstructured":"Rodr\u00edguez-S\u00e1nchez JA, Sanz MA, Blanco M, Serrano MP, Joy M, Latorre MA (2011) The influence of dietary lysine restriction during the finishing period on growth performance and carcass, meat, and fat characteristics of barrows and gilts intended for dry-cured ham production. J Anim Sci 89:3651\u20133662","journal-title":"J Anim Sci"},{"key":"2852_CR35","doi-asserted-by":"publisher","first-page":"6344","DOI":"10.3168\/jds.2014-8359","volume":"97","author":"CT Schaff","year":"2014","unstructured":"Schaff CT, Rohrbeck D, Steinhoff-Wagner J, Kanitz E, Sauerwein H, Bruckmaier RM, Hammon HM (2014) Effects of colostrum versus formula feeding on hepatic glucocorticoid and \u03b1(1)- and \u03b2(2)-adrenergic receptors in neonatal calves and their effect on glucose and lipid metabolism. J Dairy Sci 97:6344\u20136357","journal-title":"J Dairy Sci"},{"key":"2852_CR36","doi-asserted-by":"publisher","first-page":"137","DOI":"10.2527\/jas.2008-1204","volume":"88","author":"JD Schneider","year":"2010","unstructured":"Schneider JD, Tokach MD, Dritz SS, Nelssen JL, DeRouchey JM, Goodband RD (2010) Determining the effect of lysine:calorie ratio on growth performance of ten- to twenty-kilogram of body weight nursery pigs of two different genotypes. J Anim Sci 88:137\u2013146","journal-title":"J Anim Sci"},{"key":"2852_CR37","doi-asserted-by":"publisher","first-page":"10094","DOI":"10.3168\/jds.2017-13320","volume":"100","author":"CG Schwab","year":"2017","unstructured":"Schwab CG, Broderick GA (2017) A 100-Year Review: protein and amino acid nutrition in dairy cows. J Dairy Sci 100:10094\u201310112","journal-title":"J Dairy Sci"},{"key":"2852_CR38","doi-asserted-by":"publisher","first-page":"e1006916","DOI":"10.1371\/journal.ppat.1006916","volume":"14","author":"V Sekhar","year":"2018","unstructured":"Sekhar V, Pollicino T, Diaz G, Engle RE, Alayli F, Melis M, Kabat J, Tice A, Pomerenke A, Altan-Bonnet N, Zamboni F, Lusso P, Emerson SU, Farci P (2018) Infection with hepatitis C virus depends on TACSTD2, a regulator of claudin-1 and occludin highly downregulated in hepatocellular carcinoma. PloS Pathog 14:e1006916","journal-title":"PloS Pathog"},{"key":"2852_CR39","doi-asserted-by":"publisher","first-page":"e84589","DOI":"10.1371\/journal.pone.0084589","volume":"9","author":"L Servillo","year":"2014","unstructured":"Servillo L, Giovane A, Diaz G, Cautela D, Cartaldo D, Balestrieri ML (2014) Where does N(\u03b5)-trimethyllysine for the carnitine biosynthesis in mammals come from? PLoS ONE 9:e84589","journal-title":"PLoS ONE"},{"key":"2852_CR40","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1002\/biof.5520120121","volume":"12","author":"M Smriga","year":"2000","unstructured":"Smriga M, Murakami H, Mori M, Torii K (2000) Effects of l-lysine deficient diet on the hypothalamic interstitial norepinephrine and diet-induced thermogenesis in rats in vivo. BioFactors 12:137\u2013142","journal-title":"BioFactors"},{"key":"2852_CR41","doi-asserted-by":"publisher","first-page":"3583","DOI":"10.3168\/jds.S0022-0302(91)78551-2","volume":"74","author":"PJ Soest","year":"1991","unstructured":"Soest PJ, Van RJB, Lewis BA (1991) Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci 74:3583\u20133597","journal-title":"J Dairy Sci"},{"key":"2852_CR42","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1016\/j.anifeedsci.2010.02.008","volume":"157","author":"N Swanepoel","year":"2010","unstructured":"Swanepoel N, Robinson PH, Erasmus LJ (2010) Amino acid needs of lactating dairy cows: Impact of feeding lysine in a ruminally protected form on productivity of lactating dairy cows. Anim Feed Sci Technol 157:79\u201394","journal-title":"Anim Feed Sci Technol"},{"key":"2852_CR43","doi-asserted-by":"publisher","first-page":"1526","DOI":"10.2527\/jas.2007-0799","volume":"86","author":"M Takagi","year":"2008","unstructured":"Takagi M, Yonezawa T, Haga S, Shingu H, Kobayashi Y, Takahashi T, Ohtani Y, Obara Y, Katoh K (2008) Changes of activity and mRNA expression of urea cycle enzymes in the liver of developing Holstein calves. J Anim Sci 86:1526\u20131532","journal-title":"J Anim Sci"},{"key":"2852_CR44","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1158\/1535-7163.MCT-07-0565","volume":"7","author":"AB Tchagang","year":"2008","unstructured":"Tchagang AB, Tewfik AH, Derycke MS, Skubitz KM, Skubitz AP (2008) Early detection of ovarian cancer using group biomarkers. Mol Cancer Ther 7:27\u201337","journal-title":"Mol Cancer Ther"},{"key":"2852_CR45","doi-asserted-by":"publisher","first-page":"1323","DOI":"10.1017\/S0007114512003078","volume":"109","author":"T Tsurugizawa","year":"2013","unstructured":"Tsurugizawa T, Uematsu A, Uneyama H, Torii K (2013) Reversible brain response to an intragastric load of l-lysine under l-lysine depletion in conscious rats. Br J Nutr 109:1323\u20131329","journal-title":"Br J Nutr"},{"key":"2852_CR46","doi-asserted-by":"publisher","first-page":"922","DOI":"10.3168\/jds.2008-1209","volume":"92","author":"L Vels","year":"2009","unstructured":"Vels L, R\u00f8ntved CM, Bjerring M, Ingvartsen KL (2009) Cytokine and acute phase protein gene expression in repeated liver biopsies of dairy cows with a lipopolysaccharide-induced mastitis. J Dairy Sci 92:922\u2013934","journal-title":"J Dairy Sci"},{"key":"2852_CR47","doi-asserted-by":"publisher","first-page":"e0127789","DOI":"10.1371\/journal.pone.0127789","volume":"10","author":"PJ Wan","year":"2015","unstructured":"Wan PJ, Yuan SY, Tang YH, Li KL, Yang L, Fu Q, Li GQ (2015) Pathways of amino acid degradation in nilaparvata lugens (St\u00e5l) with special reference to lysine-ketoglutarate reductase\/saccharopine dehydrogenase (LKR\/SDH). PLoS ONE 10:e0127789","journal-title":"PLoS ONE"},{"key":"2852_CR48","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1038\/nrg2484","volume":"10","author":"Z Wang","year":"2009","unstructured":"Wang Z, Gerstein M, Snyder M (2009) RNA-Seq: a revolutionary tool for transcriptomics. Nat Rev Genet 10:57\u201363","journal-title":"Nat Rev Genet"},{"key":"2852_CR49","doi-asserted-by":"publisher","first-page":"224","DOI":"10.5713\/ajas.2011.11190","volume":"25","author":"J Wang","year":"2012","unstructured":"Wang J, Diao Q, Tu Y, Zhang N, Xu X (2012) The limiting sequence and proper ratio of lysine, methionine and threonine for calves fed milk replacers containing soy protein. Asian-Australas J Anim Sci 25:224\u2013233","journal-title":"Asian-Australas J Anim Sci"},{"key":"2852_CR50","doi-asserted-by":"publisher","first-page":"696","DOI":"10.2134\/jpa1999.0696","volume":"12","author":"JE Williams","year":"1999","unstructured":"Williams JE, Newell SA, Hess BW, Scholljegerdes E (1999) Influence of rumen-protected methionine and lysine on growing cattle fed forage and corn based diets. J Prod Agric 12:696\u2013701","journal-title":"J Prod Agric"},{"key":"2852_CR51","doi-asserted-by":"publisher","first-page":"1010","DOI":"10.1172\/JCI58431","volume":"122","author":"CD Wrann","year":"2012","unstructured":"Wrann CD, Eguchi J, Bozec A, Xu Z, Mikkelsen T, Gimble J, Nave H, Wagner EF, Ong SE, Rosen ED (2012) FOSL2 promotes leptin gene expression in human and mouse adipocytes. J Clin Invest 122:1010\u20131021","journal-title":"J Clin Invest"},{"key":"2852_CR52","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1016\/j.anifeedsci.2011.05.011","volume":"169","author":"F Xue","year":"2011","unstructured":"Xue F, Zhou Z, Ren L, Meng Q (2011) Influence of rumen-protected lysine supplementation on growth performance and plasma amino acid concentrations in growing cattle offered the maize stalk silage\/maize grain-based diet. Anim Feed Sci Technol 169:61\u201367","journal-title":"Anim Feed Sci Technol"},{"key":"2852_CR53","doi-asserted-by":"publisher","first-page":"1280","DOI":"10.2527\/1998.7651280x","volume":"76","author":"RA Zinn","year":"1998","unstructured":"Zinn RA, Shen Y (1998) An evaluation of ruminally degradable intake protein and metabolizable amino acid requirements of feedlot calves. J Anim Sci 76:1280\u20131289","journal-title":"J Anim Sci"}],"container-title":["Amino Acids"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00726-020-02852-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00726-020-02852-1\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00726-020-02852-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,5,4]],"date-time":"2021-05-04T23:45:26Z","timestamp":1620171926000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00726-020-02852-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5]]},"references-count":53,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2020,5]]}},"alternative-id":["2852"],"URL":"https:\/\/doi.org\/10.1007\/s00726-020-02852-1","relation":{},"ISSN":["0939-4451","1438-2199"],"issn-type":[{"value":"0939-4451","type":"print"},{"value":"1438-2199","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5]]},"assertion":[{"value":"9 July 2019","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"30 April 2020","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 May 2020","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"The authors declare no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"The experimental procedures were approved by the Animal Ethics Committee of the Chinese Academy of Agricultural sciences (committee permit number: FRI-CAAS20180110), and humane animal care and handing procedures were followed throughout the experiment.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical standards"}},{"value":"Informed consent was obtained from all individual participants included in this study.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Informed consent"}}]}}