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Previously, we found that pharmacological treatment of Becker muscular dystrophy (BMD) patients with metformin or L-citrulline resulted in antidromic effects on serum hArg and GAA concentrations, seemingly acting as an inhibitor and effector of AGAT activity, respectively. Here, we used data of this study as a model to determine <jats:italic>K<\/jats:italic><jats:sub>harg<\/jats:sub> and <jats:italic>K<\/jats:italic><jats:sub>gaa<\/jats:sub> values by using the concentrations of the participating amino acids measured in serum samples of the BMD patients. The study aimed to prove the general utility of this approach to investigate effects of amino acids and drugs on AGAT-catalyzed reactions in vivo in humans.<\/jats:p>","DOI":"10.1007\/s00726-022-03218-5","type":"journal-article","created":{"date-parts":[[2022,12,7]],"date-time":"2022-12-07T09:08:51Z","timestamp":1670404131000},"page":"203-213","update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Determination of equilibria constants of arginine:glycine amidinotransferase (AGAT)-catalyzed reactions using concentrations of circulating amino acids"],"prefix":"10.1007","volume":"55","author":[{"given":"Dimitrios","family":"Tsikas","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,12,7]]},"reference":[{"issue":"7","key":"3218_CR1","doi-asserted-by":"publisher","first-page":"1231","DOI":"10.1515\/cclm-2015-0785","volume":"54","author":"D Atzler","year":"2016","unstructured":"Atzler D, Appelbaum S, Cordts K, Ojeda FM, Wild PS, M\u00fcnzel T, Blankenberg S, B\u00f6ger RH, Blettner M, Beutel ME, Pfeiffer N, Zeller T, Lackner KJ, Schwedhelm E (2016) Reference intervals of plasma homoarginine from the German Gutenberg health study. 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Int J Mol Sci 23(18):10649. https:\/\/doi.org\/10.3390\/ijms231810649","journal-title":"Int J Mol Sci"}],"container-title":["Amino Acids"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00726-022-03218-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00726-022-03218-5\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00726-022-03218-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,23]],"date-time":"2023-02-23T18:04:24Z","timestamp":1677175464000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00726-022-03218-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,7]]},"references-count":41,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,2]]}},"alternative-id":["3218"],"URL":"https:\/\/doi.org\/10.1007\/s00726-022-03218-5","relation":{},"ISSN":["0939-4451","1438-2199"],"issn-type":[{"value":"0939-4451","type":"print"},{"value":"1438-2199","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,7]]},"assertion":[{"value":"8 July 2022","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 November 2022","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 December 2022","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare that they have no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"Ethics and health authority approvals were obtained from the local Ethics Committee (Pilotstudie zur Untersuchung der Wirksamkeit von L-Citrullin und Metformin bei Erwachsenen mit Muskeldystrophie Becker; reference No. EKBB EK17\/13) and the National Swiss Drug Agency (Swissmedic: <i>Pilotstudie bei Muskeldystrophie Becker<\/i>, reference No. 2013 DR2067, release date May 30, 2013). This pilot study entitled was conducted in accordance with the ethical principles of good clinical practice (GCP) that have their origins in the Declaration of Helsinki.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}}]}}