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Cigarette smoking is the single most important and modifiable risk factor with 5 to 20-fold susceptibility for periodontal diseases. Reverse smoking is a peculiar habit of smoking where the lit end is kept inside the mouth, which is predominant in the northern coastal districts of Andhra Pradesh. Polyamines are biologically active amines involved in tissue regeneration and modulation of inflammation. The study aimed to evaluate polyamines and check their utility as a marker in detection of periodontitis among different groups. Total polyamine levels showed significant increase in reverse smokers with periodontitis when compared to the other groups. Qualitative analysis by thin layer chromatography showed three polyamine bands with varying intensity among the different groups. Mass spectrometric and NMR analyses of the three bands identified them as N1, N8-diacetyl spermidine,\n                    <jats:italic>N<\/jats:italic>\n                    -acetyl cadaverine and lysine. Most significantly elevated levels of lysine was observed in the smoker and reverse smoker periodontitis groups when compared to healthy and non-smoker periodontitis groups. The significantly elevated levels of\n                    <jats:italic>N<\/jats:italic>\n                    -acetyl cadaverine could be responsible for the more destruction of periodontium in the reverse smoker group. Antioxidant potential decreased significantly in different smoker periodontitis groups. The present study suggests that the quantitative analysis of salivary polyamines, lysine and\n                    <jats:italic>N<\/jats:italic>\n                    -acetyl cadaverine can aid as an easy noninvasive diagnostic method for assessing the periodontal status, especially in smokers.\n                  <\/jats:p>","DOI":"10.1007\/s00726-024-03396-4","type":"journal-article","created":{"date-parts":[[2024,6,8]],"date-time":"2024-06-08T12:01:42Z","timestamp":1717848102000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Combined assessment of lysine and N-acetyl cadaverine levels assist as a potential biomarker of the smoker periodontitis"],"prefix":"10.1007","volume":"56","author":[{"given":"Md Haroon","family":"Rashid","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandhya Pavan Kumar","family":"Yellarthi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavan Kumar","family":"Yellarthi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brinda Goda Lakshmi","family":"Didugu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anitha","family":"Mamillapalli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,6,8]]},"reference":[{"issue":"1","key":"3396_CR1","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1186\/s12916-021-02035-z","volume":"19","author":"L And\u00f6rfer","year":"2021","unstructured":"And\u00f6rfer L, Holtfreter B, Weiss S, Matthes R, Pitchika V, Schmidt CO, Samietz S, Kastenm\u00fcller G, Nauck M, V\u00f6lker U, V\u00f6lzke H, Csonka LN, Suhre K, Pietzner M, Kocher T (2021) Salivary metabolites associated with a 5-year tooth loss identified in a population-based setting. 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