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Thus, our objective is to identify the specific kinds of amino acids as diagnostic biomarkers in pediatric patients with sepsis.\u00a0Serum samples were collected from patients with sepsis admitted to the pediatric intensive care unit (PICU) between January 2019 and December 2019 on the 1<jats:sup>st<\/jats:sup>, 3<jats:sup>rd<\/jats:sup> and 7<jats:sup>th<\/jats:sup> day following admission. Demographic and laboratory variables were also retrieved from the medical records specified times. Serum amino acid concentrations were detected by UPLC-MS\/MS system. PLS-DA (VIP\u2009&gt;\u20091.0) and <jats:italic>Kruskal-Wallis<\/jats:italic> test (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) were employed to identify potential biomarkers. <jats:italic>Spearman\u2019s<\/jats:italic> rank correlation analysis was conducted to find the potential association between amino acid levels and clinical features. The diagnostic utility for pediatric sepsis was assessed using receiver operating characteristic (<jats:italic>ROC<\/jats:italic>) curve analysis.\u00a0Most of amino acid contents in serum were significantly decreased in patients with sepsis, but approached normal levels by the seventh day post-diagnosis. Threonine (THR), lysine (LYS), valine (VAL) and alanine (ALA) emerged as potential biomarkers related for sepsis occurrence, though they were not associated with PELOD\/PELOD-2 scores. Moreover, alterations in serum THR, LYS and ALA were linked to complications of brain injury, and serum ALA levels were also related to sepsis-associated acute kidney injury. Further analysis revealed that ALA was significantly correlated with the <jats:italic>Glasgow<\/jats:italic> score, serum lactate and glucose levels, C-reactive protein (CRP), and other indicators for liver or kidney dysfunction. Notably, the area under the <jats:italic>ROC<\/jats:italic> curve (AUC) for ALA in distinguishing sepsis from healthy controls was 0.977 (95% <jats:italic>CI<\/jats:italic>: 0.925-1.000).\u00a0The serum amino acid profile of children with sepsis is significantly altered compared to that of healthy controls. Notably, ALA shows promise as a potential biomarker for the early diagnosis in septic children.<\/jats:p>","DOI":"10.1007\/s00726-024-03408-3","type":"journal-article","created":{"date-parts":[[2024,7,27]],"date-time":"2024-07-27T03:28:34Z","timestamp":1722050914000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Alanine, a potential amino acid biomarker of pediatric sepsis: a pilot study in PICU"],"prefix":"10.1007","volume":"56","author":[{"given":"Tiantian","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaya","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaohua","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuyun","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaodong","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunxia","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,7,27]]},"reference":[{"key":"3408_CR40","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1016\/j.mito.2014.05.007","volume":"17","author":"M Adeva-Andany","year":"2014","unstructured":"Adeva-Andany M, L\u00f3pez-Oj\u00e9n M, Funcasta-Calder\u00f3n R, Ameneiros-Rodr\u00edguez E, Donapetry-Garc\u00eda C, Vila-Altesor M, Rodr\u00edguez-Seijas J (2014) Comprehensive review on lactate metabolism in human health. 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