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We aimed to investigate this relationship using mice deficient in D-AA-related metabolic enzymes. We examined mice lacking these enzymes for approximately 900\u00a0days and the effects of altered D-AA metabolism on cancer development based on lifespan, pathological findings, and gene expression. The lifespan of female\n                    <jats:italic>DASPO<\/jats:italic>\n                    -knockout (\n                    <jats:italic>DASPO<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>\u2212\/\u2212<\/jats:italic>\n                    <\/jats:sup>\n                    ) mice was shorter than that of the other group mice; furthermore, these mice showed tumor-like masses in the liver, spleen, and small intestine. A pathological diagnosis of diffuse large B-cell lymphoma (DLBCL) was made. RNA sequencing of the liver samples showed specific alterations in the expression of 71 genes in\n                    <jats:italic>DASPO<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>\u2212\/\u2212<\/jats:italic>\n                    <\/jats:sup>\n                    mice compared with that in wild-type B6 mice;\n                    <jats:italic>RGS 1<\/jats:italic>\n                    ,\n                    <jats:italic>MTSS1<\/jats:italic>\n                    , and\n                    <jats:italic>SMARCD 1<\/jats:italic>\n                    were identified as DLBCL-related genes. Patients with DLBCL exhibiting low\n                    <jats:italic>DASPO<\/jats:italic>\n                    expression demonstrated a shorter survival period than those showing high expression. However, the role of\n                    <jats:italic>DASPO<\/jats:italic>\n                    in DLBCL development is unclear. Therefore, future research should focus on B cells.\n                    <jats:italic>DASPO<\/jats:italic>\n                    may serve as novel biomarkers and therapeutic targets in cancer.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Graphical abstract<\/jats:bold>\n                  <\/jats:p>","DOI":"10.1007\/s00726-024-03426-1","type":"journal-article","created":{"date-parts":[[2024,12,24]],"date-time":"2024-12-24T07:08:58Z","timestamp":1735024138000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Effect of D-amino acid metabolic enzyme deficiency on cancer development\u2014diffuse large B-cell lymphoma onset and gene expression analyses in DASPO-knockout mice"],"prefix":"10.1007","volume":"57","author":[{"given":"Yusuke","family":"Nakade","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasunori","family":"Iwata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenichi","family":"Harada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuharu","family":"Sato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masashi","family":"Mita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenji","family":"Hamase","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryuichi","family":"Konno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayo","family":"Hayashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taku","family":"Kobayashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuta","family":"Yamamura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tadashi","family":"Toyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsushi","family":"Tajima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Wada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,12,24]]},"reference":[{"key":"3426_CR1","doi-asserted-by":"publisher","first-page":"767070","DOI":"10.3389\/fimmu.2021.767070","volume":"12","author":"Y Bai","year":"2021","unstructured":"Bai Y, Hu M, Chen Z, Wei J, Du H (2021) Single-cell transcriptome analysis reveals RGS1 as a new marker and promoting factor for T-cell exhaustion in multiple cancers. 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