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Here, an <jats:italic>Escherichia coli<\/jats:italic> cytosol isolation procedure was developed that was devoid of detergents, strongly coordinating buffers, and EDTA. The interaction of the metal ions from these complexes with a SEC column was minimized by pre-loading the column with <jats:sup>67<\/jats:sup>ZnSO<jats:sub>4<\/jats:sub> and then monitoring <jats:sup>66<\/jats:sup>Zn and other metals by inductively coupled plasma mass spectrometry (ICP-MS) when investigating cytosolic ultrafiltration flow-through-solutions (FTSs). Endogenous cytosolic salts suppressed ESI-MS signals, making the detection of metal complexes difficult. FTSs contained ca. 80\u00a0\u00b5M Fe, 15\u00a0\u00b5M Ni, 13\u00a0\u00b5M Zn, 10\u00a0\u00b5M Cu, and 1.4\u00a0\u00b5M Mn (after correcting for dilution during cytosol isolation). FTSs exhibited 2\u20135 Fe, at least 2 Ni, 2\u20135 Zn, 2\u20134 Cu, and at least 2 Mn species with apparent masses between 300 and 5000\u00a0Da. Fe(ATP), Fe(GSH), and Zn(GSH) standards were passed through the column to assess their presence in FTS. Major LMM sulfur- and phosphorus-containing species were identified. These included reduced and oxidized glutathione, methionine, cysteine, orthophosphate, and common mono- and di-nucleotides such as ATP, ADP, AMP, and NADH. FTSs from cells grown in media supplemented with one of these metal salts exhibited increased peak intensity for the supplemented metal indicating that the size of the labile metal pools in <jats:italic>E. coli<\/jats:italic> is sensitive to the concentration of nutrient metals.<\/jats:p>","DOI":"10.1007\/s00775-021-01864-w","type":"journal-article","created":{"date-parts":[[2021,5,8]],"date-time":"2021-05-08T08:05:33Z","timestamp":1620461133000},"page":"479-494","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Low-molecular-mass labile metal pools in Escherichia coli: advances using chromatography and mass spectrometry"],"prefix":"10.1007","volume":"26","author":[{"given":"Hayley N.","family":"Brawley","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8307-9647","authenticated-orcid":false,"given":"Paul A.","family":"Lindahl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,5,8]]},"reference":[{"key":"1864_CR1","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1126\/science.1085049","volume":"300","author":"LA Finney","year":"2003","unstructured":"Finney LA, O\u2019Halloran TV (2003) Transition metal speciation in the cell; insights from the chemistry of metal ion receptors. 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