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The in vitro toxicity and genotoxicity of the metalloantibiotic were estimated toward fetal lung fibroblast (MRC-5) cells. The molecular mechanism of the <jats:bold>CIPAG<\/jats:bold> activity against MCF-7 cells was clarified by the (i) cell morphology, (ii) cell cycle arrest, (iii) mitochondrial membrane permeabilization, and (iv) by the assessment of the possible differential effect of <jats:bold>CIPAG<\/jats:bold> on estrogen receptor alpha (ER\u03b1) and estrogen receptor beta (ER\u03b2) transcriptional activation, applying luciferase reporter gene assay. Moreover, the ex vivo mechanism of <jats:bold>CIPAG<\/jats:bold> was clarified by its binding affinity toward calf thymus (CT-DNA).<\/jats:p>\n                <jats:p><jats:bold>Graphical abstract<\/jats:bold><\/jats:p>","DOI":"10.1007\/s00775-024-02048-y","type":"journal-article","created":{"date-parts":[[2024,4,6]],"date-time":"2024-04-06T05:01:24Z","timestamp":1712379684000},"page":"177-186","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Silver ciprofloxacin (CIPAG): a multitargeted metallodrug in the development of breast cancer therapy"],"prefix":"10.1007","volume":"29","author":[{"given":"Christina N.","family":"Banti","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Foteini D.","family":"Kalousi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna-Maria G.","family":"Psarra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleni E.","family":"Moushi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Demetres D.","family":"Leonidas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sotiris K.","family":"Hadjikakou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,4,6]]},"reference":[{"key":"2048_CR1","doi-asserted-by":"publisher","first-page":"2174","DOI":"10.1039\/D0BM02155E","volume":"9","author":"Y Zheng","year":"2021","unstructured":"Zheng Y, Kng J, Yang C, Hedrickc JL, Yan Yang Y (2021) Cationic polymer synergizing with chemotherapeutics and re-purposing antibiotics against cancer cells. 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