{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T13:46:40Z","timestamp":1787579200115,"version":"build-2736575974"},"reference-count":40,"publisher":"Springer Science and Business Media LLC","issue":"7","license":[{"start":{"date-parts":[[2015,5,27]],"date-time":"2015-05-27T00:00:00Z","timestamp":1432684800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Clin Oral Invest"],"published-print":{"date-parts":[[2015,9]]},"DOI":"10.1007\/s00784-015-1491-7","type":"journal-article","created":{"date-parts":[[2015,5,25]],"date-time":"2015-05-25T23:17:17Z","timestamp":1432595837000},"page":"1581-1593","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":96,"title":["Enamel matrix derivative and bone grafts for periodontal regeneration of intrabony defects. A systematic review and meta-analysis"],"prefix":"10.1007","volume":"19","author":[{"given":"M.","family":"Matarasso","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Iorio-Siciliano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Blasi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Ramaglia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. E.","family":"Salvi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Sculean","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2015,5,27]]},"reference":[{"key":"1491_CR1","doi-asserted-by":"crossref","first-page":"1809","DOI":"10.1016\/S0140-6736(05)67728-8","volume":"366","author":"BL Pihlstr\u00f6m","year":"2005","unstructured":"Pihlstr\u00f6m BL, Michalowicz BS, Johnson NW (2005) Periodontal disease. Lancet 366:1809\u20131820","journal-title":"Lancet"},{"key":"1491_CR2","first-page":"385","volume":"45","author":"A Ivanovic","year":"2014","unstructured":"Ivanovic A, Nikou G, Miron RJ, Nikolidakis D, Sculean A (2014) Which biomaterials may promote periodontal regeneration in intrabony periodontal defects? A systematic review of preclinical studies. Quintessence Int 45:385\u2013395","journal-title":"Quintessence Int"},{"key":"1491_CR3","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1111\/prd.12086","volume":"68","author":"A Sculean","year":"2015","unstructured":"Sculean A, Nikolidakis D, Nikou G, Ivanovic A, Chapple IL, Stavropoulos A (2015) Biomaterials for promoting periodontal regeneration in human intrabony defects. A systematic review. Periodontol 68:182\u2013216","journal-title":"Periodontol"},{"key":"1491_CR4","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1902\/cap.2011.110047","volume":"1","author":"A Sculean","year":"2011","unstructured":"Sculean A, Miron R, Salvi GE, Bosshardt DD (2011) Enamel matrix proteins and periodontal wound healing and regeneration. Clin Adv Periodontics 1:101\u2013117","journal-title":"Clin Adv Periodontics"},{"key":"1491_CR5","first-page":"CD003875","volume":"4","author":"M Esposito","year":"2009","unstructured":"Esposito M, Grusovin MG, Papanikolaou N, Coulthard P, Worthington HV (2009) Enamel matrix derivative (Emdogain) for periodontal tissue regeneration in intrabony defects. Cochrane Database Syst Rev 4:CD003875","journal-title":"Cochrane Database Syst Rev"},{"key":"1491_CR6","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1902\/jop.2011.110266","volume":"83","author":"R Koop","year":"2012","unstructured":"Koop R, Mareheb J, Quirynen M (2012) Periodontal regeneration with enamel matrix derivative in reconstructive periodontal therapy: a systematic review. J Periodontol 83:707\u2013720","journal-title":"J Periodontol"},{"key":"1491_CR7","doi-asserted-by":"crossref","first-page":"934","DOI":"10.1902\/jop.1993.64.10.934","volume":"64","author":"MS Tonetti","year":"1993","unstructured":"Tonetti MS, Pini-Prato G, Cortellini P (1993) Periodontal regeneration of human intrabony defects. IV. Determinants of healing response. J Periodontol 64:934\u2013940","journal-title":"J Periodontol"},{"key":"1491_CR8","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1034\/j.1600-051X.2003.10146.x","volume":"30","author":"M Silvestri","year":"2003","unstructured":"Silvestri M, Sartori S, Rasperini G, Ricci G, Rota C, Cattaneo V (2003) Comparison of intrabony defects treated with enamel matrix derivative versus guided tissue regeneration with a nonresorbable membrane. J Clin Periodontol 30:386\u2013393","journal-title":"J Clin Periodontol"},{"key":"1491_CR9","doi-asserted-by":"crossref","first-page":"1092","DOI":"10.1111\/j.1600-051X.2004.00615.x","volume":"31","author":"MS Tonetti","year":"2004","unstructured":"Tonetti MS, Fourmousis I, Suvan J, Cortellini P, Br\u00e4gger U, Lang NP, European Research Group on Periodontology (ERGOPERIO) (2004) Healing, post-operative morbidity and patient perception of outcomes following regenerative therapy of deep intrabony defects. J Clin Periodontol 31:1092\u20131098","journal-title":"J Clin Periodontol"},{"key":"1491_CR10","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1111\/j.1600-051X.2008.01295.x","volume":"35","author":"A Sculean","year":"2008","unstructured":"Sculean A, Kiss A, Miliauskaite A, Schwartz F, Arweiler NB, Hannig M (2008) Ten-years results following treatment with an enamel matrix proteins and guided tissue regeneration. J Clin Periodontol 35:817\u2013824","journal-title":"J Clin Periodontol"},{"key":"1491_CR11","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1034\/j.1600-0757.2000.2220108.x","volume":"22","author":"P Cortellini","year":"2000","unstructured":"Cortellini P, Tonetti MS (2000) Focus on intrabony defects: guided tissue regeneration. Periodontol 2000 22:104\u2013132","journal-title":"Periodontol 2000"},{"key":"1491_CR12","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1034\/j.1600-051X.2002.290407.x","volume":"29","author":"MS Tonetti","year":"2002","unstructured":"Tonetti MS, Lang NP, Cortellini P, Suvan JE, Adriaens P, Dubravec D, Fonzar A, Fourmousis I, Mayfield L, Rossi R, Silvestri M, Tiedermann C, Topoll H, Vangsted T, Wallkamm B (2002) Enamel matrix proteins in the regenerative therapy of deep intrabony defects. J Clin Periodontol 29:317\u2013325","journal-title":"J Clin Periodontol"},{"key":"1491_CR13","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1902\/jop.2010.100144","volume":"82","author":"V Iorio Siciliano","year":"2011","unstructured":"Iorio Siciliano V, Andreuccetti G, Iorio Siciliano A, Blasi A, Sculean A, Salvi GE (2011) Clinical outcomes after treatment of non-contained intrabony defects with enamel matrix derivative or guided tissue regeneration: a 12-month randomized controlled clinical trial. J Periodontol 82:62\u201371","journal-title":"J Periodontol"},{"key":"1491_CR14","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1902\/jop.2012.110656","volume":"84","author":"D Leonardis De","year":"2013","unstructured":"De Leonardis D, Paolantonio M (2013) Enamel matrix derivative alone or associated with a synthetic bone substitute in the treatment of 1- to 2-wall periodontal defects. J Periodontol 84:444\u2013455","journal-title":"J Periodontol"},{"key":"1491_CR15","doi-asserted-by":"crossref","first-page":"1342","DOI":"10.1902\/jop.2014.130420","volume":"85","author":"V Iorio-Siciliano","year":"2014","unstructured":"Iorio-Siciliano V, Andreuccetti G, Blasi A, Matarasso M, Sculean A, Salvi GE (2014) Clinical outcomes after treatment of non-contained intrabony defects using a deproteinized bovine bone mineral combined with either enamel matrix derivative or guided bone regeneration. J Periodontol 85:1342\u2013135016","journal-title":"J Periodontol"},{"key":"1491_CR16","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1007\/s00784-013-0992-5","volume":"18","author":"RJ Miron","year":"2014","unstructured":"Miron RJ, Wei L, Bosshardt DD, Buser D, Sculean A, Zhang Y (2014) Effects of enamel matrix proteins in combination with a bovine-derived natural bone mineral for the repair of bone defects. Clin Oral Investig 18:471\u201347","journal-title":"Clin Oral Investig"},{"key":"1491_CR17","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1111\/j.1600-051X.2005.00865.x","volume":"33","author":"L Trombelli","year":"2006","unstructured":"Trombelli L, Annunziata M, Belardo S, Farina R, Scabbia A, Guida L (2006) Autogenous bone graft in conjunction with enamel matrix derivative in the treatment of deep periodontal intra-osseous defects: a report of 13 consecutively treated patients. J Clin Periodontol 33:69\u201375","journal-title":"J Clin Periodontol"},{"key":"1491_CR18","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1111\/j.1600-051X.2010.01567.x","volume":"37","author":"S Yilmaz","year":"2010","unstructured":"Yilmaz S, Cakar G, Yildirim B, Sculean A (2010) Healing of two and three wall intrabony periodontal defects following treatment with an enamel matrix derivative combined with autogenous bone. J Clin Periodontol 37:544\u2013550","journal-title":"J Clin Periodontol"},{"key":"1491_CR19","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1902\/jop.2007.060142","volume":"78","author":"L Guida","year":"2007","unstructured":"Guida L, Annunaziata M, Belardo S, Farina R, Scabbia A, Trombelli L (2007) Effect of autogenous cortical bone particulate in conjunction with enamel matrix derivative in the treatment of periodontal intraosseous defects. J Periodontol 78:231\u2013238","journal-title":"J Periodontol"},{"key":"1491_CR20","doi-asserted-by":"crossref","first-page":"1309","DOI":"10.1902\/jop.2004.75.10.1309","volume":"75","author":"BS Gurinsky","year":"2004","unstructured":"Gurinsky BS, Mills MO, Melloning JT (2004) Clinical evaluation of demineralized freeze-dried bone allograft and enamel matrix derivative versus enamel matrix derivative alone for the treatment of periodontal osseous defects in human. J Periodontol 75:1309\u20131318","journal-title":"J Periodontol"},{"key":"1491_CR21","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1007\/s00784-009-0369-y","volume":"15","author":"SD Aspriello","year":"2011","unstructured":"Aspriello SD, Ferrante L, Rubini C, Piemontese M (2011) Comparative study of DFDBA in combination with enamel matrix derivative versus DFDBA alone for treatment of periodontal intrabony defects at 12-months post-surgery. Clin Oral Investig 15:225\u2013232","journal-title":"Clin Oral Investig"},{"key":"1491_CR22","doi-asserted-by":"crossref","first-page":"2273","DOI":"10.1902\/jop.2008.080259","volume":"79","author":"MJ Hoidal","year":"2008","unstructured":"Hoidal MJ, Grimard BA, Mills MP, Schoofield JD, Melloning JT, Mealey BL (2008) Clinical evaluation of demineralized freeze-dried bone allograft with and without enamel matrix derivative for the treatment of periodontal osseous defects in human. J Periodontol 79:2273\u20132280","journal-title":"J Periodontol"},{"key":"1491_CR23","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.1902\/jop.2000.71.7.1110","volume":"71","author":"V Lekovic","year":"2000","unstructured":"Lekovic V, Camargo PM, Weinlaender M, Nedic M, Aleksic Z, Kenney EB (2000) A comparison between enamel matrix proteins used alone or in combination with bovine porous bone mineral in the treatment of intrabony periodontal defects in humans. J Periodontol 71:1110\u20131116","journal-title":"J Periodontol"},{"key":"1491_CR24","doi-asserted-by":"crossref","first-page":"1157","DOI":"10.1902\/jop.2000.72.9.1157","volume":"72","author":"V Lekovic","year":"2001","unstructured":"Lekovic V, Camargo PM, Weilaender M, Vasilic N, Djordjevic M, Kenney EB (2001) The use of bovine porous bone mineral in combination with enamel matrix proteins or with autologous fibrinogen\/fibronectin system in the treatment of intrabony periodontal defects in humans. J Periodontol 72:1157\u20131163","journal-title":"J Periodontol"},{"key":"1491_CR25","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1902\/jop.2002.73.4.423","volume":"73","author":"ET Scheyer","year":"2002","unstructured":"Scheyer ET, Velasques-Plata D, Brunsvold MA, Lasho DJ, Melloning JT (2002) A clinical comparison of a bovine-derived xenograft used alone and in combination with enamel matrix derivative for treatment of periodontal osseous defects in humans. J Periodontol 73:423\u2013432","journal-title":"J Periodontol"},{"key":"1491_CR26","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1902\/jop.2002.73.4.433","volume":"73","author":"D Velasquez-Plata","year":"2002","unstructured":"Velasquez-Plata D, Scheyer ET, Melloning JT (2002) Clinical comparison of an enamel matrix derivative used alone or in combination with a bovine-derived xenograft for the treatment of periodontal osseous defects in humans. J Periodontol 73:433\u2013440","journal-title":"J Periodontol"},{"key":"1491_CR27","first-page":"47","volume":"23","author":"A Sculean","year":"2003","unstructured":"Sculean A, Windish P, Keglevich T, Chiantella GC, Gera I, Donos N (2003) Clinical and histological evaluation of human intrabony defects treated with an enamel matrix protein derivative combined with a bovine-derived xenograft. Int J Periodontics Restorative Dent 23:47\u201355","journal-title":"Int J Periodontics Restorative Dent"},{"key":"1491_CR28","doi-asserted-by":"crossref","first-page":"1725","DOI":"10.1902\/jop.2003.74.12.1725","volume":"74","author":"G Zucchelli","year":"2003","unstructured":"Zucchelli G, Amore C, Montebugnoli L, De Sanctis M (2003) Enamel matrix proteins and bovine porous mineral in the treatment of intrabony defects: a comparative controlled trial. J Periodontol 74:1725\u20131735","journal-title":"J Periodontol"},{"key":"1491_CR29","first-page":"153","volume":"28","author":"A Sculean","year":"2008","unstructured":"Sculean A, Chiantella GC, Arweiler NB, Becker J, Schwarz F, Stavropoulos A (2008) Five year clinical and histologic results following treatment of human intrabony defects with enamel matrix derivative combined with a natural bone mineral. Int J Periodontics Restorative Dent 28:153\u2013161","journal-title":"Int J Periodontics Restorative Dent"},{"key":"1491_CR30","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1111\/j.1600-051X.2011.01705.x","volume":"38","author":"P Cortellini","year":"2011","unstructured":"Cortellini P, Tonetti MS (2011) Clinical and radiographic outcomes of the modified minimally invasive technique with or without regenerative materials: a randomized-controlled trial in intra-bony defects. J Clin Periodontol 38:365\u2013373","journal-title":"J Clin Periodontol"},{"key":"1491_CR31","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1111\/j.1600-051X.2004.00635.x","volume":"32","author":"A Sculean","year":"2005","unstructured":"Sculean A, Pietruska M, Schwarz F, Willershausen B, Arweiler NB, Auschill TM (2005) Healing of human intrabony defects following regenerative periodontal therapy with enamel matrix protein derivative alone or combined with a bioactive glass. a controlled clinical study. J Clin Periodontol 32:111\u2013117","journal-title":"J Clin Periodontol"},{"key":"1491_CR32","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1007\/s00784-006-0052-5","volume":"10","author":"B Kuru","year":"2006","unstructured":"Kuru B, Ylmaz S, Argin K, Noyan U (2006) Enamel matrix derivative alone or in combination with a bioactive glass in wide intrabony defects. Clin Oral Investig 10:227\u2013234","journal-title":"Clin Oral Investig"},{"key":"1491_CR33","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1111\/j.1600-051X.2007.01084.x","volume":"34","author":"A Sculean","year":"2007","unstructured":"Sculean A, Pietruska M, Arweiler TM, Nemcovsky C (2007) Four year results of a prospective-controlled clinical study evaluating healing of intra-bony defects following treatment with an enamel matrix protein derivative alone or combined with a bioactive glass. J Clin Periodontol 34:507\u2013513","journal-title":"J Clin Periodontol"},{"key":"1491_CR34","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1111\/j.1600-051X.2006.01010.x","volume":"33","author":"I Bokan","year":"2006","unstructured":"Bokan I, Bill JS, Schlagenhauf U (2006) Primary flap closure combined with Emdogain alone or Emdogain and Cerasorb in the treatment of intra-bony defects. J Clin Periodontol 33:885\u2013893","journal-title":"J Clin Periodontol"},{"key":"1491_CR35","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1111\/j.1600-051X.2008.01217.x","volume":"35","author":"S Jepsen","year":"2008","unstructured":"Jepsen S, Topoll H, Rengers H, Heinz B, Teich M, Hoffmann T, Al-Machot E, Meyle J, Jervoe-Storm PM (2008) Clinical outcomes after treatment of intrabony defects with EMD\/synthetic bone graft or EMD alone: a multicentre randomized-controlled trial. J Clin Periodontol 35:420\u2013428","journal-title":"J Clin Periodontol"},{"key":"1491_CR36","doi-asserted-by":"crossref","first-page":"652","DOI":"10.1111\/j.1600-051X.2011.01726.x","volume":"38","author":"J Meyle","year":"2011","unstructured":"Meyle J, Hoffmann T, Topoll H, Heinz B, Al-Machot E, Jervoe-Storm PM, Meiss C, Eickholz P, Jepsen S (2011) A multi-centre randomized controlled clinical trial on the treatment of intra-bony defects with enamel matrix derivatives\/synthetic bone graft or enamel matrix derivative alone: results after 12 months. J Clin Periodontol 38:652\u2013660","journal-title":"J Clin Periodontol"},{"key":"1491_CR37","doi-asserted-by":"crossref","first-page":"1191","DOI":"10.1007\/s00784-011-0611-2","volume":"16","author":"M Pietruska","year":"2012","unstructured":"Pietruska M, Pietruski J, Nagy K, Brecx M, Arweiler NB, Sculean A (2012) Four-year results following treatment of intrabony periodontal defects with enamel matrix derivative alone or combined with a biphasic calcium phosphate. Clin Oral Investig 16:1191\u20131197","journal-title":"Clin Oral Investig"},{"key":"1491_CR38","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1034\/j.1600-051X.29.s3.15.x","volume":"29","author":"IG Needlemann","year":"2002","unstructured":"Needlemann IG (2002) A guide to systematic reviews. J Clin Periodontol 29:6\u20139","journal-title":"J Clin Periodontol"},{"key":"1491_CR39","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.1016\/j.jclinepi.2009.06.006","volume":"62","author":"A Liberati","year":"2009","unstructured":"Liberati A, Altman DG, Tetziaff J, Mulrow C, Gotzsche PC, Ioannidis JP, Clarke M, Devereaux PJ, Kleijnen J, Moher D (2009) The PRISMA statement for reporting systematic reviews and meta-analysis of studies that evaluate health care interventions: explanation and elaboration. J Clin Epidemiol 62:e1\u2013e34","journal-title":"J Clin Epidemiol"},{"key":"1491_CR40","first-page":"475","volume":"45","author":"RJ Miron","year":"2014","unstructured":"Miron RJ, GuillemetteV ZY, Chandad F, Sculean A (2014) Enamel matrix derivative in combination with bone grafts: a review of the literature. Quintessence Int 45:475\u2013487","journal-title":"Quintessence Int"}],"container-title":["Clinical Oral Investigations"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00784-015-1491-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00784-015-1491-7\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00784-015-1491-7","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,29]],"date-time":"2019-05-29T06:34:40Z","timestamp":1559111680000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00784-015-1491-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,5,27]]},"references-count":40,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2015,9]]}},"alternative-id":["1491"],"URL":"https:\/\/doi.org\/10.1007\/s00784-015-1491-7","relation":{},"ISSN":["1432-6981","1436-3771"],"issn-type":[{"value":"1432-6981","type":"print"},{"value":"1436-3771","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,5,27]]}}}