{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T21:46:53Z","timestamp":1785793613047,"version":"3.56.0"},"reference-count":44,"publisher":"Springer Science and Business Media LLC","issue":"8","license":[{"start":{"date-parts":[[2016,1,12]],"date-time":"2016-01-12T00:00:00Z","timestamp":1452556800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"funder":[{"name":"Regedent AG, Zurich, Switzerland"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Clin Oral Invest"],"published-print":{"date-parts":[[2016,11]]},"DOI":"10.1007\/s00784-016-1711-9","type":"journal-article","created":{"date-parts":[[2016,1,12]],"date-time":"2016-01-12T06:45:01Z","timestamp":1452581101000},"page":"2165-2173","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":51,"title":["In-vitro activity of sodium-hypochlorite gel on bacteria associated with periodontitis"],"prefix":"10.1007","volume":"20","author":[{"given":"Karolina","family":"Jurczyk","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandor","family":"Nietzsche","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Ender","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anton","family":"Sculean","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sigrun","family":"Eick","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,1,12]]},"reference":[{"key":"1711_CR1","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1038\/nrmicro2337","volume":"8","author":"RP Darveau","year":"2010","unstructured":"Darveau RP (2010) Periodontitis: a polymicrobial disruption of host homeostasis. Nat Rev Microbiol 8:481\u2013490","journal-title":"Nat Rev Microbiol"},{"key":"1711_CR2","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1111\/prd.12010","volume":"62","author":"R Teles","year":"2013","unstructured":"Teles R, Teles F, Frias-Lopez J, Paster B, Haffajee A (2013) Lessons learned and unlearned in periodontal microbiology. Periodontol 2000 62:95\u2013162","journal-title":"Periodontol 2000"},{"key":"1711_CR3","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1177\/154405910308200503","volume":"82","author":"PS Kumar","year":"2003","unstructured":"Kumar PS, Griffen AL, Barton JA, Paster BJ, Moeschberger ML, Leys EJ (2003) New bacterial species associated with chronic periodontitis. J Dent Res 82:338\u2013344","journal-title":"J Dent Res"},{"key":"1711_CR4","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1038\/nrmicro2873","volume":"10","author":"G Hajishengallis","year":"2012","unstructured":"Hajishengallis G, Darveau RP, Curtis MA (2012) The keystone-pathogen hypothesis. Nat Rev Microbiol 10:717\u2013725","journal-title":"Nat Rev Microbiol"},{"key":"1711_CR5","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/S1532-3382(12)70019-2","volume":"12","author":"I Sanz","year":"2012","unstructured":"Sanz I, Alonso B, Carasol M, Herrera D, Sanz M (2012) Nonsurgical treatment of periodontitis. J Evid Based Dent Pract 12:76\u201386","journal-title":"J Evid Based Dent Pract"},{"key":"1711_CR6","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1034\/j.1600-0757.2001.22250105.x","volume":"25","author":"EB Hancock","year":"2001","unstructured":"Hancock EB, Newell DH (2001) Preventive strategies and supportive treatment. Periodontol 2000 25:59\u201376","journal-title":"Periodontol 2000"},{"key":"1711_CR7","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1111\/jcpe.12026","volume":"40","author":"P Matesanz-Perez","year":"2013","unstructured":"Matesanz-Perez P, Garcia-Gargallo M, Figuero E, Bascones-Martinez A, Sanz M, Herrera D (2013) A systematic review on the effects of local antimicrobials as adjuncts to subgingival debridement, compared with subgingival debridement alone, in the treatment of chronic periodontitis. J Clin Periodontol 40:227\u2013241","journal-title":"J Clin Periodontol"},{"key":"1711_CR8","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1111\/idh.12050","volume":"12","author":"DE Slot","year":"2014","unstructured":"Slot DE, Berchier CE, Addy M, Van der Velden U, Van der Weijden GA (2014) The efficacy of chlorhexidine dentifrice or gel on plaque, clinical parameters of gingival inflammation and tooth discoloration: a systematic review. Int J Dent Hyg 12:25\u201335","journal-title":"Int J Dent Hyg"},{"key":"1711_CR9","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1034\/j.1600-0765.2002.00004.x","volume":"37","author":"J Slots","year":"2002","unstructured":"Slots J (2002) Selection of antimicrobial agents in periodontal therapy. J Periodontal Res 37:389\u2013398","journal-title":"J Periodontal Res"},{"key":"1711_CR10","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1084\/jem.27.5.627","volume":"27","author":"JH Austin","year":"1918","unstructured":"Austin JH, Taylor HD (1918) Behavior of hypochlorite and of chloramine-T Solutions in contact with necrotic and normal tissues in vivo. J Exp Med 27:627\u2013633","journal-title":"J Exp Med"},{"key":"1711_CR11","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1111\/j.1875-595X.2008.tb00354.x","volume":"58","author":"Z Mohammadi","year":"2008","unstructured":"Mohammadi Z (2008) Sodium hypochlorite in endodontics: an update review. Int Dent J 58:329\u2013341","journal-title":"Int Dent J"},{"key":"1711_CR12","doi-asserted-by":"crossref","unstructured":"Del Carpio-Perochena A, Bramante CM, de Andrade FB, Maliza AG, Cavenago BC, Marciano MA, Amoroso-Silva P, Duarte MH (2015) Antibacterial and dissolution ability of sodium hypochlorite in different pHs on multi-species biofilms. Clin Oral Investig","DOI":"10.1007\/s00784-015-1431-6"},{"key":"1711_CR13","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1046\/j.1365-2591.2001.00392.x","volume":"34","author":"DA Spratt","year":"2001","unstructured":"Spratt DA, Pratten J, Wilson M, Gulabivala K (2001) An in vitro evaluation of the antimicrobial efficacy of irrigants on biofilms of root canal isolates. Int Endod J 34:300\u2013307","journal-title":"Int Endod J"},{"key":"1711_CR14","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1902\/jop.1982.53.2.63","volume":"53","author":"KL Kalkwarf","year":"1982","unstructured":"Kalkwarf KL, Tussing GJ, Davis MJ (1982) Histologic evaluation of gingival curettage facilitated by sodium hypochlorite solution. J Periodontol 53:63\u201370","journal-title":"J Periodontol"},{"key":"1711_CR15","first-page":"23","volume":"69","author":"MD Perova","year":"1990","unstructured":"Perova MD, Lopunova Zh K, Banchenko GV, Petrosian EA (1990) A clinico-morphological assessment of the efficacy of sodium hypochlorite in the combined therapy of periodontitis. Stomatologiia (Mosk) 69:23\u201326","journal-title":"Stomatologiia (Mosk)"},{"key":"1711_CR16","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1111\/j.1875-595X.2011.00111.x","volume":"62","author":"R Nardo De","year":"2012","unstructured":"De Nardo R, Chiappe V, Gomez M, Romanelli H, Slots J (2012) Effects of 0.05% sodium hypochlorite oral rinse on supragingival biofilm and gingival inflammation. Int Dent J 62:208\u2013212","journal-title":"Int Dent J"},{"key":"1711_CR17","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1111\/jre.12219","volume":"50","author":"S Gonzalez","year":"2015","unstructured":"Gonzalez S, Cohen CL, Galvan M, Alonaizan FA, Rich SK, Slots J (2015) Gingival bleeding on probing: relationship to change in periodontal pocket depth and effect of sodium hypochlorite oral rinse. J Periodontal Res 50:397\u2013402","journal-title":"J Periodontal Res"},{"key":"1711_CR18","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1111\/jre.12151","volume":"49","author":"M Galvan","year":"2014","unstructured":"Galvan M, Gonzalez S, Cohen CL, Alonaizan FA, Chen CT, Rich SK, Slots J (2014) Periodontal effects of 0.25% sodium hypochlorite twice-weekly oral rinse. A pilot study. J Periodontal Res 49:696\u2013702","journal-title":"J Periodontal Res"},{"key":"1711_CR19","doi-asserted-by":"crossref","unstructured":"Kwasny SM, Opperman TJ (2010) Static biofilm cultures of Gram-positive pathogens grown in a microtiter format used for anti-biofilm drug discovery. Curr Protoc Pharmacol Chapter 13:Unit 13A 8","DOI":"10.1002\/0471141755.ph13a08s50"},{"key":"1711_CR20","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1590\/S1806-83242004000300012","volume":"18","author":"CV do Amorim","year":"2004","unstructured":"do Amorim CV, Aun CE, Mayer MP (2004) Susceptibility of some oral microorganisms to chlorhexidine and paramonochlorophenol. Braz Oral Res 18:242\u2013246","journal-title":"Braz Oral Res"},{"key":"1711_CR21","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1007\/s00784-011-0567-2","volume":"16","author":"S Eick","year":"2012","unstructured":"Eick S, Radakovic S, Pfister W, Nietzsche S, Sculean A (2012) Efficacy of taurolidine against periodontopathic species\u2014an in vitro study. Clin Oral Investig 16:735\u2013744","journal-title":"Clin Oral Investig"},{"key":"1711_CR22","first-page":"687","volume":"42","author":"S Eick","year":"2011","unstructured":"Eick S, Goltz S, Nietzsche S, Jentsch H, Pfister W (2011) Efficacy of chlorhexidine digluconate-containing formulations and other mouthrinses against periodontopathogenic microorganisms. Quintessence Int 42:687\u2013700","journal-title":"Quintessence Int"},{"key":"1711_CR23","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1111\/jicd.12050","volume":"5","author":"W Li","year":"2014","unstructured":"Li W, Wang RE, Finger M, Lang NP (2014) Evaluation of the antigingivitis effect of a chlorhexidine mouthwash with or without an antidiscoloration system compared to placebo during experimental gingivitis. J Investig Clin Dent 5:15\u201322","journal-title":"J Investig Clin Dent"},{"key":"1711_CR24","doi-asserted-by":"crossref","first-page":"1421","DOI":"10.1902\/jop.2009.090185","volume":"80","author":"AP Colombo","year":"2009","unstructured":"Colombo AP, Boches SK, Cotton SL, Goodson JM, Kent R, Haffajee AD, Socransky SS, Hasturk H, Van Dyke TE, Dewhirst F, Paster BJ (2009) Comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis, and periodontal health using the human oral microbe identification microarray. J Periodontol 80:1421\u20131432","journal-title":"J Periodontol"},{"key":"1711_CR25","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1111\/j.1600-0722.2004.00184.x","volume":"113","author":"JJ Doel","year":"2005","unstructured":"Doel JJ, Benjamin N, Hector MP, Rogers M, Allaker RP (2005) Evaluation of bacterial nitrate reduction in the human oral cavity. Eur J Oral Sci 113:14\u201319","journal-title":"Eur J Oral Sci"},{"key":"1711_CR26","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.freeradbiomed.2012.11.013","volume":"55","author":"V Kapil","year":"2013","unstructured":"Kapil V, Haydar SM, Pearl V, Lundberg JO, Weitzberg E, Ahluwalia A (2013) Physiological role for nitrate-reducing oral bacteria in blood pressure control. Free Radic Biol Med 55:93\u2013100","journal-title":"Free Radic Biol Med"},{"key":"1711_CR27","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1111\/j.1600-0722.2007.00493.x","volume":"115","author":"Y Takeuchi","year":"2007","unstructured":"Takeuchi Y, Guggenheim B, Filieri A, Baehni P (2007) Effect of chlorhexidine\/thymol and fluoride varnishes on dental biofilm formation in vitro. Eur J Oral Sci 115:468\u2013472","journal-title":"Eur J Oral Sci"},{"key":"1711_CR28","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1111\/j.1601-5037.2012.00556.x","volume":"11","author":"A Singh","year":"2013","unstructured":"Singh A, Daing A, Dixit J (2013) The effect of herbal, essential oil and chlorhexidine mouthrinse on de novo plaque formation. Int J Dent Hyg 11:48\u201352","journal-title":"Int J Dent Hyg"},{"key":"1711_CR29","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1111\/j.1600-0765.1978.tb00200.x","volume":"13","author":"PJ Baker","year":"1978","unstructured":"Baker PJ, Coburn RA, Genco RJ, Evans RT (1978) The in vitro inhibition of microbial growth and plaque formation by surfactant drugs. J Periodontal Res 13:474\u2013485","journal-title":"J Periodontal Res"},{"key":"1711_CR30","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1007\/978-1-4939-2854-5_5","volume":"1333","author":"I Vandecandelaere","year":"2016","unstructured":"Vandecandelaere I, Van Acker H, Coenye T (2016) A microplate-based system as in vitro model of biofilm growth and quantification. Methods Mol Biol 1333:53\u201366","journal-title":"Methods Mol Biol"},{"key":"1711_CR31","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.mimet.2007.11.010","volume":"72","author":"E Peeters","year":"2008","unstructured":"Peeters E, Nelis HJ, Coenye T (2008) Comparison of multiple methods for quantification of microbial biofilms grown in microtiter plates. J Microbiol Methods 72:157\u2013165","journal-title":"J Microbiol Methods"},{"key":"1711_CR32","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1093\/femsre\/fuv015","volume":"39","author":"L Hobley","year":"2015","unstructured":"Hobley L, Harkins C, MacPhee CE, Stanley-Wall NR (2015) Giving structure to the biofilm matrix: an overview of individual strategies and emerging common themes. FEMS Microbiol Rev 39:649\u2013669","journal-title":"FEMS Microbiol Rev"},{"key":"1711_CR33","doi-asserted-by":"crossref","unstructured":"Okshevsky M, Meyer RL (2013) The role of extracellular DNA in the establishment, maintenance and perpetuation of bacterial biofilms. Crit Rev Microbiol","DOI":"10.3109\/1040841X.2013.841639"},{"key":"1711_CR34","doi-asserted-by":"crossref","first-page":"e00497-13","DOI":"10.1128\/mBio.00497-13","volume":"4","author":"BW Peterson","year":"2013","unstructured":"Peterson BW, van der Mei HC, Sjollema J, Busscher HJ, Sharma PK (2013) A distinguishable role of eDNA in the viscoelastic relaxation of biofilms. MBio 4:e00497-13","journal-title":"MBio"},{"key":"1711_CR35","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.copbio.2014.12.002","volume":"33","author":"M Okshevsky","year":"2015","unstructured":"Okshevsky M, Regina VR, Meyer RL (2015) Extracellular DNA as a target for biofilm control. Curr Opin Biotechnol 33:73\u201380","journal-title":"Curr Opin Biotechnol"},{"key":"1711_CR36","doi-asserted-by":"crossref","first-page":"51","DOI":"10.17796\/jcpd.39.1.e72570275387527r","volume":"39","author":"S Gomez","year":"2014","unstructured":"Gomez S, Bravo P, Morales R, Romero A, Oyarzun A (2014) Resin penetration in artificial enamel carious lesions after using sodium hypochlorite as a deproteinization agent. J Clin Pediatr Dent 39:51\u201356","journal-title":"J Clin Pediatr Dent"},{"key":"1711_CR37","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.joen.2009.10.020","volume":"36","author":"K Zhang","year":"2010","unstructured":"Zhang K, Kim YK, Cadenaro M, Bryan TE, Sidow SJ, Loushine RJ, Ling JQ, Pashley DH, Tay FR (2010) Effects of different exposure times and concentrations of sodium hypochlorite\/ethylenediaminetetraacetic acid on the structural integrity of mineralized dentin. J Endod 36:105\u2013109","journal-title":"J Endod"},{"key":"1711_CR38","first-page":"358","volume":"12","author":"AM Prince","year":"1992","unstructured":"Prince AM, Andrus L (1992) PCR: how to kill unwanted DNA. Biotechniques 12:358\u2013360","journal-title":"Biotechniques"},{"key":"1711_CR39","doi-asserted-by":"crossref","unstructured":"Darzynkiewicz Z, Juan G, Srour EF (2004) Differential staining of DNA and RNA. Curr Protoc Cytom Chapter 7:Unit 7 3","DOI":"10.1002\/0471142956.cy0703s30"},{"key":"1711_CR40","doi-asserted-by":"crossref","unstructured":"Ali Mohammed MM, Nerland AH, Al-Haroni M, Bakken V (2013) Characterization of extracellular polymeric matrix, and treatment of Fusobacterium nucleatum and Porphyromonas gingivalis biofilms with DNase I and proteinase K. J Oral Microbiol 5","DOI":"10.3402\/jom.v5i0.20015"},{"key":"1711_CR41","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1146\/annurev.micro.112408.134306","volume":"64","author":"K Lewis","year":"2010","unstructured":"Lewis K (2010) Persister cells. Annu Rev Microbiol 64:357\u2013372","journal-title":"Annu Rev Microbiol"},{"key":"1711_CR42","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1007\/s00784-004-0271-6","volume":"8","author":"S Fure","year":"2004","unstructured":"Fure S, Lingstrom P (2004) Evaluation of the chemomechanical removal of dentine caries in vivo with a new modified Carisolv gel. Clin Oral Investig 8:139\u2013144","journal-title":"Clin Oral Investig"},{"key":"1711_CR43","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1590\/S0103-64402006000300007","volume":"17","author":"DC Grisi","year":"2006","unstructured":"Grisi DC, Salvador SL, Marcantonio RA (2006) Efficacy of Carisolv as an adjunctive therapy to scaling and root planing on subgingival calculus removal. Braz Dent J 17:213\u2013218","journal-title":"Braz Dent J"},{"key":"1711_CR44","first-page":"91","volume":"31","author":"AV Imbronito","year":"2011","unstructured":"Imbronito AV, Okuda O, Del Cioppo CV, Arana-Chavez VE (2011) Scanning electron microscopy in vitro study on the effect of Carisolv application on periodontally diseased root surfaces. Int J Periodontics Restorative Dent 31:91\u201395","journal-title":"Int J Periodontics Restorative Dent"}],"container-title":["Clinical Oral Investigations"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00784-016-1711-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00784-016-1711-9\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00784-016-1711-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00784-016-1711-9","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,3]],"date-time":"2019-09-03T13:03:29Z","timestamp":1567515809000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00784-016-1711-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,1,12]]},"references-count":44,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2016,11]]}},"alternative-id":["1711"],"URL":"https:\/\/doi.org\/10.1007\/s00784-016-1711-9","relation":{},"ISSN":["1432-6981","1436-3771"],"issn-type":[{"value":"1432-6981","type":"print"},{"value":"1436-3771","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,1,12]]}}}