{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T19:41:40Z","timestamp":1787773300953,"version":"build-2784847793"},"reference-count":60,"publisher":"Springer Science and Business Media LLC","issue":"6","license":[{"start":{"date-parts":[[2020,5,6]],"date-time":"2020-05-06T00:00:00Z","timestamp":1588723200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2020,5,6]],"date-time":"2020-05-06T00:00:00Z","timestamp":1588723200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"funder":[{"name":"School of Dentistry,Shahid Beheshti University of Medical Sciences","award":["6210"],"award-info":[{"award-number":["6210"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Clin Oral Invest"],"published-print":{"date-parts":[[2020,6]]},"DOI":"10.1007\/s00784-019-03075-9","type":"journal-article","created":{"date-parts":[[2020,5,6]],"date-time":"2020-05-06T14:03:55Z","timestamp":1588773835000},"page":"2099-2108","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Identification of microorganisms in irreversible pulpitis and primary endodontic infections with respect to clinical and radiographic findings"],"prefix":"10.1007","volume":"24","author":[{"given":"Nazanin","family":"Zargar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hengameh","family":"Ashraf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. M. Amin","family":"Marashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad","family":"Sabeti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alireza","family":"Aziz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,5,6]]},"reference":[{"key":"3075_CR1","doi-asserted-by":"publisher","first-page":"340","DOI":"10.1016\/0030-4220(65)90166-0","volume":"20","author":"S Kakehashi","year":"1965","unstructured":"Kakehashi S, Stanley HR, Fitzgerald RJ (1965) The effects of surgical exposures of dental pulps in germ-free and conventional laboratory rats. Oral Surgery, Oral Medicine, Oral Pathology 20:340\u2013349. https:\/\/doi.org\/10.1016\/0030-4220(65)90166-0","journal-title":"Oral Surgery, Oral Medicine, Oral Pathology"},{"key":"3075_CR2","doi-asserted-by":"publisher","first-page":"475","DOI":"10.1111\/j.1600-0722.1981.tb01711.x","volume":"89","author":"\u00c5JR M\u00f6ller","year":"1981","unstructured":"M\u00f6ller \u00c5JR, Fabricius L, Dahl\u00e9n G, \u00d6hman AE, Heyden G (1981) Influence on periapical tissues of indigenous oral bacteria and necrotic pulp tissue in monkeys. European Journal of Oral Sciences 89:475\u2013484. https:\/\/doi.org\/10.1111\/j.1600-0722.1981.tb01711.x","journal-title":"European Journal of Oral Sciences"},{"key":"3075_CR3","doi-asserted-by":"publisher","first-page":"870","DOI":"10.1016\/j.tripleo.2009.01.044","volume":"107","author":"JF Siqueira Jr","year":"2009","unstructured":"Siqueira JF Jr, Rocas IN (2009) Community as the unit of pathogenicity: an emerging concept as to the microbial pathogenesis of apical periodontitis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 107:870\u2013878. https:\/\/doi.org\/10.1016\/j.tripleo.2009.01.044","journal-title":"Oral Surg Oral Med Oral Pathol Oral Radiol Endod"},{"key":"3075_CR4","doi-asserted-by":"publisher","first-page":"391","DOI":"10.1016\/S0099-2399(82)80092-7","volume":"8","author":"M Cvek","year":"1982","unstructured":"Cvek M, Cleaton-Jones PE, Austin JC, Andreasen JO (1982) Pulp reactions to exposure after experimental crown fractures or grinding in adult monkeys. J Endod 8:391\u2013397. https:\/\/doi.org\/10.1016\/S0099-2399(82)80092-7","journal-title":"J Endod"},{"key":"3075_CR5","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1046\/j.0902-0055.2003.00116.x","volume":"19","author":"BPFA Gomes","year":"2004","unstructured":"Gomes BPFA, Pinheiro ET, Gad\u00ea-Neto CR, Sousa ELR, Ferraz CCR, Zaia AA, Teixeira FB, Souza-Filho FJ (2004) Microbiological examination of infected dental root canals. Oral Microbiol Immunol 19:71\u201376. https:\/\/doi.org\/10.1046\/j.0902-0055.2003.00116.x","journal-title":"Oral Microbiol Immunol"},{"key":"3075_CR6","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1111\/j.1601-0825.2009.01607.x","volume":"16","author":"NB Parahitiyawa","year":"2010","unstructured":"Parahitiyawa NB, Scully C, Leung WK, Yam WC, Jin LJ, Samaranayake LP (2010) Exploring the oral bacterial flora: current status and future directions. Oral Dis 16:136\u2013145. https:\/\/doi.org\/10.1111\/j.1601-0825.2009.01607.x","journal-title":"Oral Dis"},{"key":"3075_CR7","doi-asserted-by":"publisher","first-page":"1080","DOI":"10.1016\/j.joen.2018.04.003","volume":"44","author":"JM Shin","year":"2018","unstructured":"Shin JM, Luo T, Lee KH, Guerreiro D, Botero TM, McDonald NJ, Rickard AH (2018) Deciphering endodontic microbial communities by next-generation sequencing. J Endod 44:1080\u20131087. https:\/\/doi.org\/10.1016\/j.joen.2018.04.003","journal-title":"J Endod"},{"key":"3075_CR8","doi-asserted-by":"publisher","first-page":"638","DOI":"10.1097\/01.DON.0000125875.88377.85","volume":"30","author":"JF Siqueira","year":"2004","unstructured":"Siqueira JF, R\u00f4\u00e7as IN, Alves FRF, Santos KRN (2004) Selected endodontic pathogens in the apical third of infected root canals: a molecular investigation. J Endod 30:638\u2013643. https:\/\/doi.org\/10.1097\/01.DON.0000125875.88377.85","journal-title":"J Endod"},{"issue":"9","key":"3075_CR9","doi-asserted-by":"publisher","first-page":"1136","DOI":"10.1016\/j.joen.2013.05.001","volume":"39","author":"Bo-Young Hong","year":"2013","unstructured":"Hong B-Y, Lee T-K, Lim S-M, Chang SW, Park J, Han SH, Zhu Q, Safavi KE, Fouad AF, Kum KY (2013) Microbial analysis in primary and persistent endodontic infections by using pyrosequencing. J Endod 39: 1136-1140. https:\/\/doi.org\/10.1016\/j.joen.2013.05.001","journal-title":"Journal of Endodontics"},{"key":"3075_CR10","doi-asserted-by":"publisher","first-page":"670","DOI":"10.1016\/j.joen.2013.10.005","volume":"40","author":"C Tennert","year":"2014","unstructured":"Tennert C, Fuhrmann M, Wittmer A, Karygianni L, Altenburger MJ, Pelz K, Hellwig E, Al-Ahmad A (2014) New bacterial composition in primary and persistent\/secondary endodontic infections with respect to clinical and radiographic findings. J Endod 40:670\u2013677. https:\/\/doi.org\/10.1016\/j.joen.2013.10.005","journal-title":"J Endod"},{"issue":"13","key":"3075_CR11","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1111\/j.1600-0757.1997.tb00098.x","volume":"2000","author":"PNR Nair","year":"1997","unstructured":"Nair PNR (1997) Apical periodontitis: a dynamic encounter between root canal infection and host response. Periodontology 2000(13):121\u2013148. https:\/\/doi.org\/10.1111\/j.1600-0757.1997.tb00098.x","journal-title":"Periodontology"},{"key":"3075_CR12","doi-asserted-by":"publisher","first-page":"422","DOI":"10.1111\/iej.12469","volume":"49","author":"ME Lysakowska","year":"2016","unstructured":"Lysakowska ME, Ciebiada-Adamiec A, Sienkiewicz M, Sokolowski J, Banaszek K (2016) The cultivable microbiota of primary and secondary infected root canals, their susceptibility to antibiotics and association with the signs and symptoms of infection. Int Endod J 49:422\u2013430. https:\/\/doi.org\/10.1111\/iej.12469","journal-title":"Int Endod J"},{"issue":"5","key":"3075_CR13","doi-asserted-by":"publisher","first-page":"541","DOI":"10.1016\/j.joen.2008.02.004","volume":"34","author":"Luciana M. Sassone","year":"2008","unstructured":"Sassone LM, Fidel RA, Faveri M, Guerra R, Figueiredo L, Fidel SR, Feres M (2008) A microbiological profile of symptomatic teeth with primary endodontic infections. J Endod 34: 541-545. https:\/\/doi.org\/10.1016\/j.joen.2008.02.004","journal-title":"Journal of Endodontics"},{"key":"3075_CR14","doi-asserted-by":"publisher","first-page":"1206","DOI":"10.1016\/j.joen.2011.05.026","volume":"37","author":"IN Rocas","year":"2011","unstructured":"Rocas IN, Siqueira JF Jr, Debelian GJ (2011) Analysis of symptomatic and asymptomatic primary root canal infections in adult Norwegian patients. J Endod 37:1206\u20131212. https:\/\/doi.org\/10.1016\/j.joen.2011.05.026","journal-title":"J Endod"},{"key":"3075_CR15","doi-asserted-by":"publisher","first-page":"302","DOI":"10.1016\/j.joen.2018.11.017","volume":"45","author":"J Zheng","year":"2019","unstructured":"Zheng J, Wu Z, Niu K, Xie Y, Hu X, Fu J, Tian D, Fu K, Zhao B, Kong W, Sun C, Wu L (2019) Microbiome of deep dentinal caries from reversible pulpitis to irreversible pulpitis. J Endod 45:302\u2013309.e301. https:\/\/doi.org\/10.1016\/j.joen.2018.11.017","journal-title":"J Endod"},{"key":"3075_CR16","doi-asserted-by":"publisher","first-page":"2095","DOI":"10.1007\/s00784-014-1198-1","volume":"18","author":"FC Martinho","year":"2014","unstructured":"Martinho FC, Leite FR, Nascimento GG, Cirelli JA, Gomes BP (2014) Clinical investigation of bacterial species and endotoxin in endodontic infection and evaluation of root canal content activity against macrophages by cytokine production. Clin Oral Investig 18:2095\u20132102. https:\/\/doi.org\/10.1007\/s00784-014-1198-1","journal-title":"Clin Oral Investig"},{"key":"3075_CR17","doi-asserted-by":"publisher","first-page":"744","DOI":"10.1067\/moe.2000.106576","volume":"89","author":"JF Siqueira","year":"2000","unstructured":"Siqueira JF, R\u00f4\u00e7as IN, Souto R, Md U, Colombo AP (2000) Checkerboard DNA-DNA hybridization analysis of endodontic infections. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology 89:744\u2013748. https:\/\/doi.org\/10.1067\/moe.2000.106576","journal-title":"Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology"},{"key":"3075_CR18","doi-asserted-by":"publisher","first-page":"3223","DOI":"10.1128\/jcm.40.9.3223-3231.2002","volume":"40","author":"AF Fouad","year":"2002","unstructured":"Fouad AF, Barry J, Caimano M, Clawson M, Zhu Q, Carver R, Hazlett K, Radolf JD (2002) PCR-based identification of bacteria associated with endodontic infections. J Clin Microbiol 40:3223\u20133231. https:\/\/doi.org\/10.1128\/jcm.40.9.3223-3231.2002","journal-title":"J Clin Microbiol"},{"issue":"7","key":"3075_CR19","doi-asserted-by":"publisher","first-page":"922","DOI":"10.1016\/j.joen.2011.04.007","volume":"37","author":"Adriana C. Ribeiro","year":"2011","unstructured":"Ribeiro AC, Matarazzo F, Faveri M, Zezell DM, Mayer MPA (2011) Exploring bacterial diversity of endodontic microbiota by cloning and sequencing 16S rRNA. J Endod 37: 922-926. https:\/\/doi.org\/10.1016\/j.joen.2011.04.007","journal-title":"Journal of Endodontics"},{"key":"3075_CR20","doi-asserted-by":"publisher","first-page":"3314","DOI":"10.1128\/JCM.43.7.3314-3319.2005","volume":"43","author":"JF Siqueira","year":"2005","unstructured":"Siqueira JF, R\u00f4\u00e7as IN (2005) Uncultivated phylotypes and newly named species associated with primary and persistent endodontic infections. J Clin Microbiol 43:3314. https:\/\/doi.org\/10.1128\/JCM.43.7.3314-3319.2005","journal-title":"J Clin Microbiol"},{"key":"3075_CR21","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1111\/j.1399-302X.2005.00227.x","volume":"20","author":"F Foschi","year":"2005","unstructured":"Foschi F, Cavrini F, Montebugnoli L, Stashenko P, Sambri V, Prati C (2005) Detection of bacteria in endodontic samples by polymerase chain reaction assays and association with defined clinical signs in Italian patients. Oral Microbiol Immunol 20:289\u2013295. https:\/\/doi.org\/10.1111\/j.1399-302X.2005.00227.x","journal-title":"Oral Microbiol Immunol"},{"key":"3075_CR22","doi-asserted-by":"publisher","first-page":"390","DOI":"10.1111\/j.1399-302X.2007.00376.x","volume":"22","author":"L Sassone","year":"2007","unstructured":"Sassone L, Fidel R, Figueiredo L, Fidel S, Faveri M, Feres M (2007) Evaluation of the microbiota of primary endodontic infections using checkerboard DNA\u2013DNA hybridization. Oral Microbiol Immunol 22:390\u2013397. https:\/\/doi.org\/10.1111\/j.1399-302X.2007.00376.x","journal-title":"Oral Microbiol Immunol"},{"key":"3075_CR23","doi-asserted-by":"publisher","first-page":"384","DOI":"10.1111\/j.1399-302X.2008.00440.x","volume":"23","author":"B Blome","year":"2008","unstructured":"Blome B, Braun A, Sobarzo V, Jepsen S (2008) Molecular identification and quantification of bacteria from endodontic infections using real-time polymerase chain reaction. Oral Microbiol Immunol 23:384\u2013390. https:\/\/doi.org\/10.1111\/j.1399-302X.2008.00440.x","journal-title":"Oral Microbiol Immunol"},{"key":"3075_CR24","volume-title":"Glossary of endodontic terms","author":"P Eleazer","year":"2012","unstructured":"Eleazer P, Glickman G, McClanahan S, Webb T, Jusrman B (2012) Glossary of endodontic terms. Editorial AAE, Chicago"},{"key":"3075_CR25","doi-asserted-by":"publisher","first-page":"338","DOI":"10.1177\/154405910308200503","volume":"82","author":"PS Kumar","year":"2003","unstructured":"Kumar PS, Griffen AL, Barton JA, Paster BJ, Moeschberger ML, Leys EJ (2003) New bacterial species associated with chronic periodontitis. J Dent Res 82:338\u2013344. https:\/\/doi.org\/10.1177\/154405910308200503","journal-title":"J Dent Res"},{"key":"3075_CR26","doi-asserted-by":"publisher","first-page":"1687","DOI":"10.1128\/aem.69.3.1687-1694.2003","volume":"69","author":"MM Brinig","year":"2003","unstructured":"Brinig MM, Lepp PW, Ouverney CC, Armitage GC, Relman DA (2003) Prevalence of bacteria of division TM7 in human subgingival plaque and their association with disease. Appl Environ Microbiol 69:1687\u20131694. https:\/\/doi.org\/10.1128\/aem.69.3.1687-1694.2003","journal-title":"Appl Environ Microbiol"},{"key":"3075_CR27","doi-asserted-by":"publisher","first-page":"697","DOI":"10.1128\/jb.173.2.697-703.1991","volume":"173","author":"WG Weisburg","year":"1991","unstructured":"Weisburg WG, Barns SM, Pelletier DA, Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697\u2013703. https:\/\/doi.org\/10.1128\/jb.173.2.697-703.1991","journal-title":"J Bacteriol"},{"key":"3075_CR28","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1097\/00004770-200403000-00004","volume":"30","author":"JC Baumgartner","year":"2004","unstructured":"Baumgartner JC, Siqueira JF Jr, Xia T, Rocas IN (2004) Geographical differences in bacteria detected in endodontic infections using polymerase chain reaction. J Endod 30:141\u2013144. https:\/\/doi.org\/10.1097\/00004770-200403000-00004","journal-title":"J Endod"},{"key":"3075_CR29","doi-asserted-by":"publisher","first-page":"641","DOI":"10.1016\/j.tripleo.2004.07.009","volume":"99","author":"JF Siqueira","year":"2005","unstructured":"Siqueira JF, Jung IY, Rocas IN, Lee CY (2005) Differences in prevalence of selected bacterial species in primary endodontic infections from two distinct geographic locations. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 99:641\u2013647. https:\/\/doi.org\/10.1016\/j.tripleo.2004.07.009","journal-title":"Oral Surg Oral Med Oral Pathol Oral Radiol Endod"},{"key":"3075_CR30","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1590\/S0103-64402007000400001","volume":"18","author":"JF Siqueira Jr","year":"2007","unstructured":"Siqueira JF Jr, Rocas IN (2007) Bacterial pathogenesis and mediators in apical periodontitis. Braz Dent J 18:267\u2013280. https:\/\/doi.org\/10.1590\/S0103-64402007000400001","journal-title":"Braz Dent J"},{"key":"3075_CR31","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1111\/j.1747-4477.2008.00120.x","volume":"34","author":"M Kovacevic","year":"2008","unstructured":"Kovacevic M, Tamarut T, Jonjic N, Braut A, Kovacevic M (2008) The transition from pulpitis to periapical periodontitis in dogs\u2019 teeth. Aust Endod J 34:12\u201318. https:\/\/doi.org\/10.1111\/j.1747-4477.2008.00120.x","journal-title":"Aust Endod J"},{"key":"3075_CR32","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1002\/ar.1092220310","volume":"222","author":"CL Kimberly","year":"1988","unstructured":"Kimberly CL, Byers MR (1988) Inflammation of rat molar pulp and periodontium causes increased calcitonin gene-related peptide and axonal sprouting. Anat Rec 222:289\u2013300. https:\/\/doi.org\/10.1002\/ar.1092220310","journal-title":"Anat Rec"},{"key":"3075_CR33","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1016\/j.biotechadv.2014.10.013","volume":"33","author":"L SMdF","year":"2015","unstructured":"SMdF L, de P\u00e1dua GM, MGdC S, MdS F, Franco OL, TMB R (2015) Antimicrobial peptide-based treatment for endodontic infections\u2014biotechnological innovation in endodontics. Biotechnology Advances 33:203\u2013213. https:\/\/doi.org\/10.1016\/j.biotechadv.2014.10.013","journal-title":"Biotechnology Advances"},{"key":"3075_CR34","doi-asserted-by":"publisher","first-page":"1132","DOI":"10.1016\/j.joen.2018.03.009","volume":"44","author":"C Widmer","year":"2018","unstructured":"Widmer C, Skutas J, Easson C, Lopez JV, Torneck C, Flax M, Sayin TC (2018) Culture-independent characterization of the microbiome of healthy pulp. J Endod 44:1132\u20131139.e1132. https:\/\/doi.org\/10.1016\/j.joen.2018.03.009","journal-title":"J Endod"},{"key":"3075_CR35","doi-asserted-by":"publisher","first-page":"1330","DOI":"10.1177\/00220345740530060501","volume":"53","author":"ES Akpata","year":"1974","unstructured":"Akpata ES (1974) Total viable count of microorganisms in the infected dental pulp. J Dent Res 53:1330\u20131333. https:\/\/doi.org\/10.1177\/00220345740530060501","journal-title":"J Dent Res"},{"issue":"2","key":"3075_CR36","doi-asserted-by":"publisher","first-page":"e12312","DOI":"10.1111\/jicd.12312","volume":"9","author":"Smita Pattanshetty","year":"2018","unstructured":"Pattanshetty S, Kotrashetti VS, Bhat K, Nayak RS, Somannavar P, Pujar M (2018) Multiplex polymerase chain reaction detection of selected bacterial species from symptomatic and asymptomatic non-vital teeth with primary endodontic infections. J Investig Clin Dent. https:\/\/doi.org\/10.1111\/jicd.12312","journal-title":"Journal of Investigative and Clinical Dentistry"},{"key":"3075_CR37","doi-asserted-by":"publisher","first-page":"889","DOI":"10.1016\/j.joen.2012.03.021","volume":"38","author":"LM Sassone","year":"2012","unstructured":"Sassone LM, Fidel RA, Faveri M, Figueiredo L, Fidel SR, Feres M (2012) A microbiological profile of unexposed and exposed pulp space of primary endodontic infections by checkerboard DNA-DNA hybridization. J Endod 38:889\u2013893. https:\/\/doi.org\/10.1016\/j.joen.2012.03.021","journal-title":"J Endod"},{"key":"3075_CR38","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1007\/s00784-006-0085-9","volume":"11","author":"JC Machado de Oliveira","year":"2007","unstructured":"Machado de Oliveira JC, Gama TG, Siqueira JF Jr, Rocas IN, Peixoto RS, Rosado AS (2007) On the use of denaturing gradient gel electrophoresis approach for bacterial identification in endodontic infections. Clin Oral Investig 11:127\u2013132. https:\/\/doi.org\/10.1007\/s00784-006-0085-9","journal-title":"Clin Oral Investig"},{"key":"3075_CR39","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1016\/S1348-8643(13)00026-8","volume":"11","author":"P Dhandapani","year":"2014","unstructured":"Dhandapani P, MuraliKannan M, Anandkumar B, Maruthamuthu S, Manoharan SP (2014) Electrochemistry of calcium precipitating bacteria in orthodontic wire. Int J Oral Sci 11:22\u201329. https:\/\/doi.org\/10.1016\/S1348-8643(13)00026-8","journal-title":"Int J Oral Sci"},{"key":"3075_CR40","doi-asserted-by":"publisher","first-page":"e49576","DOI":"10.1371\/journal.pone.0049576","volume":"7","author":"AC Anderson","year":"2012","unstructured":"Anderson AC, Hellwig E, Vespermann R, Wittmer A, Schmid M, Karygianni L, Al-Ahmad A (2012) Comprehensive analysis of secondary dental root canal infections: a combination of culture and culture-independent approaches reveals new insights. PLOS ONE 7:e49576. https:\/\/doi.org\/10.1371\/journal.pone.0049576","journal-title":"PLOS ONE"},{"key":"3075_CR41","doi-asserted-by":"publisher","first-page":"147","DOI":"10.1016\/0003-9969(91)90077-8","volume":"36","author":"CL Hahn","year":"1991","unstructured":"Hahn CL, Falkler WA, Minah GE (1991) Microbiological studies of carious dentine from human teeth with irreversible pulpitis. Arch Oral Biol 36:147\u2013153. https:\/\/doi.org\/10.1016\/0003-9969(91)90077-8","journal-title":"Arch Oral Biol"},{"key":"3075_CR42","doi-asserted-by":"publisher","first-page":"1698","DOI":"10.1128\/jcm.40.5.1698-1704.2002","volume":"40","author":"FE Martin","year":"2002","unstructured":"Martin FE, Nadkarni MA, Jacques NA, Hunter N (2002) Quantitative microbiological study of human carious dentine by culture and real-time PCR: association of anaerobes with histopathological changes in chronic pulpitis. J Clin Microbiol 40:1698\u20131704. https:\/\/doi.org\/10.1128\/jcm.40.5.1698-1704.2002","journal-title":"J Clin Microbiol"},{"key":"3075_CR43","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1111\/j.1747-4477.2003.tb00538.x","volume":"29","author":"FE Martin","year":"2003","unstructured":"Martin FE (2003) Carious pulpitis: microbiological and histopathological considerations. Australian Endodontic Journal 29:134\u2013137. https:\/\/doi.org\/10.1111\/j.1747-4477.2003.tb00538.x","journal-title":"Australian Endodontic Journal"},{"key":"3075_CR44","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1111\/j.1600-051X.1988.tb01561.x","volume":"15","author":"AJ van Winkelhoff","year":"1988","unstructured":"van Winkelhoff AJ, van Steenbergen TJM, de Graaff J (1988) The role of black-pigmented Bacteroides in human oral infections. J Clin Periodontol 15:145\u2013155. https:\/\/doi.org\/10.1111\/j.1600-051X.1988.tb01561.x","journal-title":"J Clin Periodontol"},{"key":"3075_CR45","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1097\/01.DON.0000133157.60731.3F","volume":"31","author":"CAS de Souza","year":"2005","unstructured":"de Souza CAS, Teles RP, Souto R, Chaves MAE, Colombo APV (2005) Endodontic therapy associated with calcium hydroxide as an intracanal dressing: microbiologic evaluation by the checkerboard DNA-DNA hybridization technique. J Endod 31:79\u201383. https:\/\/doi.org\/10.1097\/01.DON.0000133157.60731.3F","journal-title":"J Endod"},{"issue":"10","key":"3075_CR46","doi-asserted-by":"publisher","first-page":"1617","DOI":"10.1016\/j.joen.2010.07.001","volume":"36","author":"Isabela N. R\u00f4\u00e7as","year":"2010","unstructured":"R\u00f4\u00e7as IN, Alves FRF, Santos AL, Rosado AS, Siqueira Jr JF (2010) Apical root canal microbiota as determined by reverse-capture checkerboard analysis of cryogenically ground root samples from teeth with apical periodontitis. J Endod 36: 1617-1621. https:\/\/doi.org\/10.1016\/j.joen.2010.07.001","journal-title":"Journal of Endodontics"},{"key":"3075_CR47","doi-asserted-by":"publisher","first-page":"390","DOI":"10.1097\/00004770-200306000-00002","volume":"29","author":"JF Siqueira Jr","year":"2003","unstructured":"Siqueira JF Jr, Rocas IN (2003) Bacteroides forsythus in primary endodontic infections as detected by nested PCR. J Endod 29:390\u2013393. https:\/\/doi.org\/10.1097\/00004770-200306000-00002","journal-title":"J Endod"},{"key":"3075_CR48","doi-asserted-by":"publisher","first-page":"433","DOI":"10.1016\/s0099-2399(97)80297-x","volume":"23","author":"G Conrads","year":"1997","unstructured":"Conrads G, Gharbia SE, Gulabivala K, Lampert F, Shah HN (1997) The use of a 16s rDNA directed PCR for the detection of endodontopathogenic bacteria. J Endod 23:433\u2013438. https:\/\/doi.org\/10.1016\/s0099-2399(97)80297-x","journal-title":"J Endod"},{"key":"3075_CR49","doi-asserted-by":"publisher","first-page":"468","DOI":"10.1067\/moe.2001.114379","volume":"91","author":"IN Rocas","year":"2001","unstructured":"Rocas IN, Siqueira JF Jr, Santos KR, Coelho AM (2001) \u201cRed complex\u201d (Bacteroides forsythus, Porphyromonas gingivalis, and Treponema denticola) in endodontic infections: a molecular approach. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 91:468\u2013471. https:\/\/doi.org\/10.1067\/moe.2001.114379","journal-title":"Oral Surg Oral Med Oral Pathol Oral Radiol Endod"},{"key":"3075_CR50","doi-asserted-by":"publisher","first-page":"1049","DOI":"10.1016\/j.joen.2007.05.017","volume":"33","author":"BP Gomes","year":"2007","unstructured":"Gomes BP, Montagner F, Jacinto RC, Zaia AA, Ferraz CC, Souza-Filho FJ (2007) Polymerase chain reaction of Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia in primary endodontic infections. J Endod 33:1049\u20131052. https:\/\/doi.org\/10.1016\/j.joen.2007.05.017","journal-title":"J Endod"},{"key":"3075_CR51","doi-asserted-by":"publisher","first-page":"e69","DOI":"10.1590\/1807-3107bor-2018.vol32.0069","volume":"32","author":"B Gomes","year":"2018","unstructured":"Gomes B, Herrera DR (2018) Etiologic role of root canal infection in apical periodontitis and its relationship with clinical symptomatology. Braz Oral Res 32:e69. https:\/\/doi.org\/10.1590\/1807-3107bor-2018.vol32.0069","journal-title":"Braz Oral Res"},{"key":"3075_CR52","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1111\/j.1600-0722.1982.tb01536.x","volume":"90","author":"L Fabricious","year":"1982","unstructured":"Fabricious L, Dahlen G, \u00d6hman AE, M\u00f6ller AJR (1982) Predominant indigenous oral bacteria isolated from infected root canals after varied times of closure. European Journal of Oral Sciences 90:134\u2013144. https:\/\/doi.org\/10.1111\/j.1600-0722.1982.tb01536.x","journal-title":"European Journal of Oral Sciences"},{"key":"3075_CR53","doi-asserted-by":"publisher","first-page":"653","DOI":"10.1128\/mmbr.00024-07","volume":"71","author":"HK Kuramitsu","year":"2007","unstructured":"Kuramitsu HK, He X, Lux R, Anderson MH, Shi W (2007) Interspecies interactions within oral microbial communities. Microbiol Mol Biol Rev 71:653\u2013670. https:\/\/doi.org\/10.1128\/mmbr.00024-07","journal-title":"Microbiol Mol Biol Rev"},{"key":"3075_CR54","doi-asserted-by":"publisher","first-page":"1871","DOI":"10.1007\/s00784-016-1980-3","volume":"21","author":"IF Persoon","year":"2017","unstructured":"Persoon IF, Buijs MJ, Ozok AR, Crielaard W, Krom BP, Zaura E, Brandt BW (2017) The mycobiome of root canal infections is correlated to the bacteriome. Clin Oral Investig 21:1871\u20131881. https:\/\/doi.org\/10.1007\/s00784-016-1980-3","journal-title":"Clin Oral Investig"},{"key":"3075_CR55","doi-asserted-by":"publisher","first-page":"695","DOI":"10.1097\/00004770-200012000-00003","volume":"26","author":"JC Baumgartner","year":"2000","unstructured":"Baumgartner JC, Watts CM, Xia T (2000) Occurrence of Candida albicans in infections of endodontic origin. J Endod 26:695\u2013698. https:\/\/doi.org\/10.1097\/00004770-200012000-00003","journal-title":"J Endod"},{"issue":"1","key":"3075_CR56","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1016\/j.joen.2008.09.017","volume":"35","author":"Hong Li","year":"2009","unstructured":"Li H, Chen V, Chen Y, Baumgartner JC, Machida CA (2009) Herpesviruses in endodontic pathoses: association of Epstein-Barr virus with irreversible pulpitis and apical periodontitis. J Endod 35: 23-29. https:\/\/doi.org\/10.1016\/j.joen.2008.09.017","journal-title":"Journal of Endodontics"},{"key":"3075_CR57","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1034\/j.1399-302X.2003.00055.x","volume":"18","author":"M Sabeti","year":"2003","unstructured":"Sabeti M, Valles Y, Nowzari H, Simon JH, Kermani-Arab V, Slots J (2003) Cytomegalovirus and Epstein\u2013Barr virus DNA transcription in endodontic symptomatic lesions. Oral Microbiol Immunol 18:104\u2013108. https:\/\/doi.org\/10.1034\/j.1399-302X.2003.00055.x","journal-title":"Oral Microbiol Immunol"},{"key":"3075_CR58","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1097\/00004770-200402000-00001","volume":"30","author":"M Sabeti","year":"2004","unstructured":"Sabeti M, Slots J (2004) Herpesviral-bacterial coinfection in periapical pathosis. J Endod 30:69\u201372. https:\/\/doi.org\/10.1097\/00004770-200402000-00001","journal-title":"J Endod"},{"key":"3075_CR59","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1080\/19424396.2011.12221912","volume":"39","author":"J Slots","year":"2011","unstructured":"Slots J (2011) Herpesvirus periodontitis: infection beyond biofilm. J Calif Dent Assoc 39:393\u2013399","journal-title":"J Calif Dent Assoc"},{"key":"3075_CR60","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1016\/j.joen.2016.10.006","volume":"43","author":"S Zhong","year":"2017","unstructured":"Zhong S, Naqvi A, Bair E, Nares S, Khan AA (2017) Viral microRNAs identified in human dental pulp. J Endod 43:84\u201389. https:\/\/doi.org\/10.1016\/j.joen.2016.10.006","journal-title":"J Endod"}],"container-title":["Clinical Oral Investigations"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00784-019-03075-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00784-019-03075-9\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00784-019-03075-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,30]],"date-time":"2023-09-30T19:11:47Z","timestamp":1696101107000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00784-019-03075-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,6]]},"references-count":60,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2020,6]]}},"alternative-id":["3075"],"URL":"https:\/\/doi.org\/10.1007\/s00784-019-03075-9","relation":{},"ISSN":["1432-6981","1436-3771"],"issn-type":[{"value":"1432-6981","type":"print"},{"value":"1436-3771","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,6]]},"assertion":[{"value":"23 February 2019","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"13 September 2019","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 May 2020","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"The authors declare that they have no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"In this article, all procedures involving human participants were in accordance with the ethical standards of the Research Ethics Committee of Dental Research Center, Shahid Beheshti University of Medical Sciences (IR.SBMU.IRDS.REC.1394.23)","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"Informed consent was obtained from all individual participants included in the study.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Informed consent"}}]}}