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Fungal cells were exposed for 1\u2032, 5\u2032, or 15\u2032 to 4 different benzydamine concentrations (0.075 to 0.6%) to 2 mouthwashes (MoWs) containing benzydamine and to a placebo MoW (without benzydamine). Treated cells were tested for adhesion (90 min) and biofilm formation (24-h assay). Next, 24- and 48-h-old biofilms were exposed to benzydamine and MoWs to assess regrowth and persistence, respectively. The effects of benzydamine, MoWs containing benzydamine, and placebo on different biofilm stages were quantified by bioluminescence assay and by the production of quorum sensing (QS) molecules.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Benzydamine and MoWs containing benzydamine impaired <jats:italic>C. albicans<\/jats:italic> ability to adhere and form biofilm, counteracted <jats:italic>C. albicans<\/jats:italic> persistence and regrowth, and impaired a 48-h-old biofilm. Some of these effects paralleled with alterations in QS molecule secretion.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Our results show for the first time that benzydamine and MoWs containing benzydamine impair <jats:italic>C. albicans<\/jats:italic> capacity to form biofilm and counteract biofilm persistence and regrowth.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Clinical relevance<\/jats:title>\n                <jats:p>Benzydamine and MoWs containing benzydamine capacity to affect <jats:italic>C. albicans<\/jats:italic> biofilm provides an interesting tool to prevent and treat oral candidiasis. Likely, restraining <jats:italic>C. albicans<\/jats:italic> colonization through daily oral hygiene may counteract colonization and persistence by other critical oral pathogens, such as <jats:italic>Streptococcus mutans<\/jats:italic>, whose increased virulence has been linked to the presence of <jats:italic>C. albicans<\/jats:italic> biofilm.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1007\/s00784-021-04330-8","type":"journal-article","created":{"date-parts":[[2022,1,23]],"date-time":"2022-01-23T00:03:55Z","timestamp":1642896235000},"page":"3613-3625","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Effects of benzydamine and mouthwashes containing benzydamine on Candida albicans adhesion, biofilm formation, regrowth, and persistence"],"prefix":"10.1007","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9845-7399","authenticated-orcid":false,"given":"Andrea","family":"Ardizzoni","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giorgia","family":"Boaretto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Pericolini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego","family":"Pinetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Capezzone de Joannon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia","family":"Durando","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorella","family":"Ragni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabetta","family":"Blasi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,1,23]]},"reference":[{"key":"4330_CR1","doi-asserted-by":"publisher","first-page":"488","DOI":"10.1016\/j.tripleo.2009.11.026","volume":"109","author":"GR Thompson","year":"2010","unstructured":"Thompson GR, Patel PK, Kirkpatrick WR et al (2010) Oropharyngeal candidiasis in the era of antiretroviral therapy. 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