{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T00:32:12Z","timestamp":1767141132231,"version":"build-2238731810"},"reference-count":29,"publisher":"Springer Science and Business Media LLC","issue":"10","license":[{"start":{"date-parts":[[2022,6,8]],"date-time":"2022-06-08T00:00:00Z","timestamp":1654646400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2022,6,8]],"date-time":"2022-06-08T00:00:00Z","timestamp":1654646400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Clin Oral Invest"],"DOI":"10.1007\/s00784-022-04574-y","type":"journal-article","created":{"date-parts":[[2022,6,8]],"date-time":"2022-06-08T10:03:04Z","timestamp":1654682584000},"page":"6235-6243","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Assessing immuno-expression of p53 protein and TP 53 gene amplification in histologically negative surgical margins of oral squamous cell carcinoma patients and normal oral mucosa"],"prefix":"10.1007","volume":"26","author":[{"given":"Mamata S.","family":"Kamat","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rudrayya S.","family":"Puranik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. Bhagavan","family":"Das Rai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. R.","family":"Patil","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7246-5497","authenticated-orcid":false,"given":"Shankargouda","family":"Patil","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,6,8]]},"reference":[{"issue":"2","key":"4574_CR1","doi-asserted-by":"publisher","first-page":"102","DOI":"10.1177\/1066896914565022","volume":"23","author":"PV Angadi","year":"2015","unstructured":"Angadi PV, Patil PV, Hallikeri K, Mallapur MD, Hallikerimath S, Kale AD (2015) Tumor budding is an independent prognostic factor for prediction of lymph node metastasis in oral squamous cell carcinoma. Int J Surg Pathol 23(2):102\u2013110. https:\/\/doi.org\/10.1177\/1066896914565022","journal-title":"Int J Surg Pathol"},{"issue":"3","key":"4574_CR2","first-page":"152","volume":"26","author":"Y More","year":"2013","unstructured":"More Y, D\u2019Cruz AK (2013) Oral cancer: review of current management strategies. Natl Med J India 26(3):152\u2013158","journal-title":"Natl Med J India"},{"issue":"5","key":"4574_CR3","doi-asserted-by":"publisher","first-page":"685","DOI":"10.1016\/j.tripleo.2008.07.002","volume":"106","author":"LR Oliveira","year":"2008","unstructured":"Oliveira LR, Ribeiro-Silva A, Costa JPO, Sim\u00f5es AL, Di MMAS, Zucoloto S (2008) Prognostic factors and survival analysis in a sample of oral squamous cell carcinoma patients. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 106(5):685\u2013695. https:\/\/doi.org\/10.1016\/j.tripleo.2008.07.002","journal-title":"Oral Surg Oral Med Oral Pathol Oral Radiol Endod"},{"issue":"6","key":"4574_CR4","doi-asserted-by":"publisher","first-page":"700","DOI":"10.1016\/j.ijom.2015.11.014","volume":"45","author":"DB Jelovac","year":"2016","unstructured":"Jelovac DB, Tepav\u010devi\u0107 Z, Nikoli\u0107 N et al (2016) The amplification of c-erb-B2 in cancer-free surgical margins is a predictor of poor outcome in oral squamous cell carcinoma. Int J Oral Maxillofac Surg 45(6):700\u2013705. https:\/\/doi.org\/10.1016\/j.ijom.2015.11.014","journal-title":"Int J Oral Maxillofac Surg"},{"issue":"11","key":"4574_CR5","doi-asserted-by":"publisher","first-page":"3614","DOI":"10.1158\/1078-0432.CCR-03-0631","volume":"10","author":"VMM van Houten","year":"2004","unstructured":"van Houten VMM, Leemans CR, Kummer JA et al (2004) Molecular diagnosis of surgical margins and local recurrence in head and neck cancer patients: a prospective study. Clin Cancer Res 10(11):3614\u20133620. https:\/\/doi.org\/10.1158\/1078-0432.CCR-03-0631","journal-title":"Clin Cancer Res"},{"issue":"7","key":"4574_CR6","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1016\/j.ooe.2005.02.010","volume":"41","author":"B Mognetti","year":"2005","unstructured":"Mognetti B, Trione E, Corvetti G et al (2005) \u0394Np63\u03b1 as early indicator of malignancy in surgical margins of an oral squamous cell carcinoma. Oral Oncol Extra 41(7):129\u2013131. https:\/\/doi.org\/10.1016\/j.ooe.2005.02.010","journal-title":"Oral Oncol Extra"},{"issue":"1","key":"4574_CR7","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1111\/j.1600-0714.2008.00715.x","volume":"38","author":"A Bilde","year":"2009","unstructured":"Bilde A, von Buchwald C, Dabelsteen E, Therkildsen MH, Dabelsteen S (2009) Molecular markers in the surgical margin of oral carcinomas. J Oral Pathol Med 38(1):72\u201378. https:\/\/doi.org\/10.1111\/j.1600-0714.2008.00715.x","journal-title":"J Oral Pathol Med"},{"issue":"2","key":"4574_CR8","doi-asserted-by":"publisher","first-page":"166","DOI":"10.3892\/mco.2015.689","volume":"4","author":"J Singh","year":"2016","unstructured":"Singh J, Jayaraj R, Baxi S et al (2016) Immunohistochemical expression levels of p53 and eIF4E markers in histologically negative surgical margins, and their association with the clinical outcome of patients with head and neck squamous cell carcinoma. Mol Clin Oncol 4(2):166\u2013172. https:\/\/doi.org\/10.3892\/mco.2015.689","journal-title":"Mol Clin Oncol"},{"key":"4574_CR9","doi-asserted-by":"publisher","first-page":"1465","DOI":"10.2147\/CMAR.S192500","volume":"11","author":"X-H Yang","year":"2019","unstructured":"Yang X-H, Ding L, Fu Y et al (2019) p53-positive expression in dysplastic surgical margins is a predictor of tumor recurrence in patients with early oral squamous cell carcinoma. Cancer Manag Res 11:1465\u20131472. https:\/\/doi.org\/10.2147\/CMAR.S192500","journal-title":"Cancer Manag Res"},{"issue":"3","key":"4574_CR10","doi-asserted-by":"publisher","first-page":"240","DOI":"10.1016\/j.oraloncology.2011.10.018","volume":"48","author":"AC de Carvalho","year":"2012","unstructured":"de Carvalho AC, Kowalski LP, Campos AHJFM, Soares FA, Carvalho AL, Vettore AL (2012) Clinical significance of molecular alterations in histologically negative surgical margins of head and neck cancer patients. Oral Oncol 48(3):240\u2013248. https:\/\/doi.org\/10.1016\/j.oraloncology.2011.10.018","journal-title":"Oral Oncol"},{"issue":"12","key":"4574_CR11","doi-asserted-by":"publisher","first-page":"4070","DOI":"10.1245\/s10434-019-07645-9","volume":"26","author":"MM Li","year":"2019","unstructured":"Li MM, Puram SV, Silverman DA, Old MO, Rocco JW, Kang SY (2019) Margin analysis in head and neck cancer: state of the art and future directions. Ann Surg Oncol 26(12):4070\u20134080. https:\/\/doi.org\/10.1245\/s10434-019-07645-9","journal-title":"Ann Surg Oncol"},{"issue":"1","key":"4574_CR12","doi-asserted-by":"publisher","first-page":"78","DOI":"10.4103\/jomfp.JOMFP_185_16","volume":"22","author":"AK Shah","year":"2018","unstructured":"Shah AK (2018) Postoperative pathologic assessment of surgical margins in oral cancer: a contemporary review. J Oral Maxillofac Pathol 22(1):78\u201385. https:\/\/doi.org\/10.4103\/jomfp.JOMFP_185_16","journal-title":"J Oral Maxillofac Pathol"},{"issue":"7","key":"4574_CR13","doi-asserted-by":"publisher","first-page":"1835","DOI":"10.26717\/bjstr.2017.01.000562","volume":"1","author":"K Payne","year":"2017","unstructured":"Payne K (2017) Factors influencing the status of the surgical margin in the resection of oral squamous cell carcinoma. Biomed J Sci Tech Res 1(7):1835\u20131838. https:\/\/doi.org\/10.26717\/bjstr.2017.01.000562","journal-title":"Biomed J Sci Tech Res"},{"issue":"3","key":"4574_CR14","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1016\/j.coms.2017.03.002","volume":"29","author":"DJ Clark","year":"2017","unstructured":"Clark DJ, Mao L (2017) Understanding the surgical margin: a molecular assessment. Oral Maxillofac Surg Clin North Am 29(3):245\u2013258. https:\/\/doi.org\/10.1016\/j.coms.2017.03.002","journal-title":"Oral Maxillofac Surg Clin North Am"},{"issue":"1","key":"4574_CR15","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1136\/jcp.52.1.75","volume":"52","author":"EJ Adams","year":"1999","unstructured":"Adams EJ, Green JA, Clark AH, Youngson JH (1999) Comparison of different scoring systems for immunohistochemical staining. J Clin Pathol 52(1):75\u201377. https:\/\/doi.org\/10.1136\/jcp.52.1.75","journal-title":"J Clin Pathol"},{"issue":"4","key":"4574_CR16","first-page":"1107","volume":"54","author":"D Stoic\u0103nescu","year":"2013","unstructured":"Stoic\u0103nescu D, Andreescu N, Belengeanu A, Meszaros N, Cornianu M (2013) Assessment of p53 and HER-2\/neu genes status and protein products in oral squamous cell carcinomas. Rom J Morphol Embryol 54(4):1107\u20131113","journal-title":"Rom J Morphol Embryol"},{"key":"4574_CR17","doi-asserted-by":"publisher","unstructured":"Elizabeth van Pelt-Verkuil Alex van Belkum John P. Hays (2008) Principles and technical aspects of PCR amplification. https:\/\/doi.org\/10.1007\/978-1-40206241-4","DOI":"10.1007\/978-1-40206241-4"},{"issue":"4","key":"4574_CR18","first-page":"1017","volume":"12","author":"KG Heah","year":"2011","unstructured":"Heah KG, Hassan MIA, Huat SC (2011) p53 Expression as a marker of microinvasion in oral squamous cell carcinoma. Asian Pac J Cancer Prev 12(4):1017\u20131022","journal-title":"Asian Pac J Cancer Prev"},{"issue":"3","key":"4574_CR19","doi-asserted-by":"publisher","first-page":"222","DOI":"10.1016\/s1368-8375(02)00163-x","volume":"39","author":"M Gasco","year":"2003","unstructured":"Gasco M, Crook T (2003) The p53 network in head and neck cancer. Oral Oncol 39(3):222\u2013231. https:\/\/doi.org\/10.1016\/s1368-8375(02)00163-x","journal-title":"Oral Oncol"},{"issue":"9","key":"4574_CR20","doi-asserted-by":"publisher","first-page":"413","DOI":"10.1034\/j.1600-0714.2000.290901.x","volume":"29","author":"K Nylander","year":"2000","unstructured":"Nylander K, Dabelsteen E, Hall PA (2000) The p53 molecule and its prognostic role in squamous cell carcinomas of the head and neck. J Oral Pathol Med 29(9):413\u2013425. https:\/\/doi.org\/10.1034\/j.1600-0714.2000.290901.x","journal-title":"J Oral Pathol Med"},{"issue":"7","key":"4574_CR21","doi-asserted-by":"publisher","first-page":"429","DOI":"10.1056\/NEJM199502163320704","volume":"332","author":"JA Brennan","year":"1995","unstructured":"Brennan JA, Mao L, Hruban RH et al (1995) Molecular assessment of histopathological staging in squamous-cell carcinoma of the head and neck. N Engl J Med 332(7):429\u2013435. https:\/\/doi.org\/10.1056\/NEJM199502163320704","journal-title":"N Engl J Med"},{"issue":"2","key":"4574_CR22","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1111\/jop.12334","volume":"45","author":"X Wang","year":"2016","unstructured":"Wang X, Chen S, Chen X, Zhang C, Liang X (2016) Tumor-related markers in histologically normal margins correlate with locally recurrent oral squamous cell carcinoma: a retrospective study. J Oral Pathol Med 45(2):83\u201388. https:\/\/doi.org\/10.1111\/jop.12334","journal-title":"J Oral Pathol Med"},{"issue":"4\u20135","key":"4574_CR23","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1016\/j.oraloncology.2008.06.002","volume":"45","author":"S Warnakulasuriya","year":"2009","unstructured":"Warnakulasuriya S (2009) Global epidemiology of oral and oropharyngeal cancer. Oral Oncol 45(4\u20135):309\u2013316. https:\/\/doi.org\/10.1016\/j.oraloncology.2008.06.002","journal-title":"Oral Oncol"},{"key":"4574_CR24","first-page":"166","volume-title":"Pathology and genetics of head and neck tumours","author":"PJE Slootweg","year":"2005","unstructured":"Slootweg PJE (2005) Tumors of oral cavity and oropharynx: introduction. In: John LB, W. Eveson Peter Reichart David Sidransky, (eds) Pathology and genetics of head and neck tumours, 2005th edn. IARCPress International Agency for Research on Cancer (IARC) 69008 Lyon, France, pp 166\u2013175","edition":"2005"},{"key":"4574_CR25","unstructured":"Prabhu SR (1992) Oral diseases in the tropics. Oxford University Press."},{"issue":"4","key":"4574_CR26","doi-asserted-by":"publisher","first-page":"587","DOI":"10.4103\/0973-1482.126452","volume":"9","author":"A Chandra","year":"2013","unstructured":"Chandra A, Singh A, Sebastian BT, Agnihotri A, Bali R, Verma PK (2013) Oral squamous cell carcinomas in age distinct population: a comparison of p53 immunoexpression. J Cancer Res Ther 9(4):587\u2013591. https:\/\/doi.org\/10.4103\/0973-1482.126452","journal-title":"J Cancer Res Ther"},{"key":"4574_CR27","doi-asserted-by":"publisher","unstructured":"S\u00e1 C, Silva L, Cardoso S, Aguiar M, Carmo M (2006) p53 Immunoexpression in oral squamous cell carcinomas from different anatomical sites: a comparative study. Int J Morphol. 24. https:\/\/doi.org\/10.4067\/S0717-95022006000300018","DOI":"10.4067\/S0717-95022006000300018"},{"issue":"2","key":"4574_CR28","doi-asserted-by":"publisher","first-page":"132","DOI":"10.1002\/(SICI)1096-9896(200006)191:2<132::AID-PATH605>3.0.CO;2-E","volume":"191","author":"IB Cruz","year":"2000","unstructured":"Cruz IB, Meijer CJ, Snijders PJ, Snow GB, Walboomers JM, van Der Waal I (2000) p53 immunoexpression in non-malignant oral mucosa adjacent to oral squamous cell carcinoma: potential consequences for clinical management. J Pathol 191(2):132\u2013137. https:\/\/doi.org\/10.1002\/(SICI)1096-9896(200006)191:2%3c132::AID-PATH605%3e3.0.CO;2-E","journal-title":"J Pathol"},{"issue":"2","key":"4574_CR29","doi-asserted-by":"publisher","first-page":"98","DOI":"10.1136\/jcp.55.2.98","volume":"55","author":"I Cruz","year":"2002","unstructured":"Cruz I, Napier SS, van der Waal I et al (2002) Suprabasal p53 immunoexpression is strongly associated with high grade dysplasia and risk for malignant transformation in potentially malignant oral lesions from Northern Ireland. J Clin Pathol 55(2):98\u2013104. https:\/\/doi.org\/10.1136\/jcp.55.2.98","journal-title":"J Clin Pathol"}],"updated-by":[{"DOI":"10.1007\/s00784-022-04834-x","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2022,12,23]],"date-time":"2022-12-23T00:00:00Z","timestamp":1671753600000}}],"container-title":["Clinical Oral Investigations"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00784-022-04574-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00784-022-04574-y\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00784-022-04574-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,24]],"date-time":"2022-12-24T02:07:18Z","timestamp":1671847638000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00784-022-04574-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,8]]},"references-count":29,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2022,10]]}},"alternative-id":["4574"],"URL":"https:\/\/doi.org\/10.1007\/s00784-022-04574-y","relation":{},"ISSN":["1436-3771"],"issn-type":[{"value":"1436-3771","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,8]]},"assertion":[{"value":"19 February 2022","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"30 May 2022","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 June 2022","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 December 2022","order":4,"name":"change_date","label":"Change Date","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Correction","order":5,"name":"change_type","label":"Change Type","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"A Correction to this paper has been published:","order":6,"name":"change_details","label":"Change Details","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"https:\/\/doi.org\/10.1007\/s00784-022-04834-x","URL":"https:\/\/doi.org\/10.1007\/s00784-022-04834-x","order":7,"name":"change_details","label":"Change Details","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All procedures performed in studies involving human participants were following the ethical standards of the institutional and\/or national research committee and with the Helsinki declaration.and its later amendments or comparable ethical standards. The research protocol was approved by the Department of Histopathology, Karnataka Cancer Therapy and Research Institute Ref- KCTRI- EC-01\/2017, KCTRI Hubli, Karnataka, India.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"Informed consent was obtained from all individual participants included in this study.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Informed consent"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}