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After 3\u00a0months, the perforation site was assessed radiographically (vertical bone density), histologically (inflammatory cell count, epithelial proliferation, cementum and bone deposition) and immunohistochemically (OPN and TRAP antibodies localisation). Data collected were statistically analysed using SPSS software at a 0.05 significance level.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The MTA\u2009+\u2009PRF and MTA\u2009+\u2009CGF groups demonstrated significantly more bone formation, OPN immunolocalisation and fewer inflammatory cell counts than MTA group. MTA, MTA\u2009+\u2009PRF and MTA\u2009+\u2009CGF groups showed significantly favourable radiographic, histological and immunohistochemical healing features than the positive control, especially in non-contaminated subdivisions, that significantly showed better features than the contaminated subdivisions (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.001).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The use CGF and PRF as a matrix beneath MTA in FP repair in dog\u2019s teeth is promising as it could increase hard and soft tissue regeneration in non-contaminated and contaminated perforations.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Clinical relevance<\/jats:title>\n                    <jats:p>The repair of FP is challenging especially when associated with contaminated inter-radicular bone loss. Radiographic, histological and immunohistochemical comprehensive evaluation of the root and surrounding attachment apparatus response to different perforation repair protocols could give a predictable clinical outcome.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s00784-023-05131-x","type":"journal-article","created":{"date-parts":[[2023,7,27]],"date-time":"2023-07-27T17:02:15Z","timestamp":1690477335000},"page":"5103-5119","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Radiographic and immuno-histochemical evaluation of root perforation repair using MTA with or without platelet-rich fibrin or concentrated growth factors as an internal matrix in dog\u2019s teeth: in vivo animal study"],"prefix":"10.1007","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5724-9251","authenticated-orcid":false,"given":"Dalia Abd-Allah","family":"Mohamed","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3481-796X","authenticated-orcid":false,"given":"Safinaz AbdelFatah","family":"Abdelwahab","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6626-1770","authenticated-orcid":false,"given":"Rania Hanafi","family":"Mahmoud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1856-6526","authenticated-orcid":false,"given":"Rasha Mohamed","family":"Taha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,7,27]]},"reference":[{"key":"5131_CR1","doi-asserted-by":"publisher","first-page":"1137","DOI":"10.1016\/j.joen.2017.02.001","volume":"43","author":"LA Silva","year":"2017","unstructured":"Silva LA, Pieroni KA, Nelson-Filho P, Silva RA, Hernand\u00e9zGat\u00f3n P, Lucisano MP (2017) Furcation perforation: periradicular tissue response to biodentine as a repair material by histopathologic and indirect immunofluorescence analyses. 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