{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T15:27:23Z","timestamp":1786980443268,"version":"build-2736575974"},"reference-count":56,"publisher":"Springer Science and Business Media LLC","issue":"6","license":[{"start":{"date-parts":[[2024,5,25]],"date-time":"2024-05-25T00:00:00Z","timestamp":1716595200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,5,25]],"date-time":"2024-05-25T00:00:00Z","timestamp":1716595200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"University of Health Sciences"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Clin Oral Invest"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Mechano-sensitive odontoblast cells, which sense mechanical loading and various stresses in the tooth structure, synthesize early signaling molecules such as prostaglandin E2 (PGE2) and nitric oxide (NO) as an adaptive response. It is thought that these synthesized molecules can be used for the diagnosis and treatment of periodontal and periapical diseases. The aim of this study was to investigate the relationship between the severity of apical periodontitis (AP) and chronic periodontitis (CP) and serum (s) TNF-\u03b1, IL-10, PGE2 and NO levels, as well as PGE2 and NO levels in gingival crevicular fluid (GCF) samples.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials &amp; methods<\/jats:title>\n                    <jats:p>\n                      A total of 185 subjects were divided into three categories: AP group (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200985), CP group (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200950) and healthy control group (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200950). The AP group was divided into 3 subgroups according to abscess scoring (AS-PAI 1, 2 and 3) based on the periapical index. The CP group was divided into 4 subgroups according to the periodontitis staging system (PSS1, 2,3 and 4). After recording the demographic and clinical characteristics of all participants, serum (s) and gingival crevicular fluid (GCF) samples were taken. TNF-\u03b1, IL-10, PGE2 and NO levels were measured in these samples.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Unlike serum measurements (sTNF-\u03b1, sIL-10, sNO and sPGE2), GCF-NO and GCF-PGE levels of the AP group were significantly higher than the control group in relation to abscess formation (54.4\u2009\u00b1\u200956.3 vs. 22.5\u2009\u00b1\u200912.6 \u00b5mol\/mL,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001 and 100\u2009\u00b1\u200998 vs. 41\u2009\u00b1\u200928 ng\/L,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001, respectively). Confirming this, the GCF-NO and GCF-PGE levels of the AS-PAI 1 group, in which abscesses have not yet formed, were found to be lower than those in AS-PAI 2 and 3, which are characterized by abscess formation [(16.7(3.7-117.8), 32.9(11.8-212.8) and 36.9(4.3-251.6) \u00b5mol\/mL,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090,0131; 46.0(31.4\u2013120.0), 69.6(40.3-424.2) and 74.4(32.1\u2013471.0) ng\/L,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090,0020, respectively]. Consistent with the increase in PSS, the levels of sTNF [29.8 (8.2-105.5) vs. 16.7(6.3\u201337.9) pg\/mL,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001], sIL-10 [542(106\u20131326) vs. 190(69\u2013411) pg\/mL,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001], sNO [182.1(36.3\u2013437) vs. 57.0(15.9\u2013196) \u00b5mol\/mL,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001], sPGE2 [344(82-1298) vs. 100(35-1178) ng\/L,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001], GCF-NO [58.9\u2009\u00b1\u200933.6 vs. 22.5\u2009\u00b1\u200912.6 ng\/L,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001] and GCF-PGE2 [ 99(37\u2013365) vs. 30(13\u2013119),\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001] in the CP group were higher than the control group. Comparison ROC analysis revealed that the GCF-PGE2 test had the best diagnostic value for both AP and CP (sensitivity: 94.1 and 88.0; specificity: 64.0 and 78.0, respectively;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>GCF-PE2 and GCF-NO have high diagnostic value in the determination of AP and CP, and can be selected as targets to guide treatment. In addition, the measurements of PGE2 and NO in GCF can be used as an important predictor of pulpal necrosis leading to abscess in patients with AP.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Clinical relevance<\/jats:title>\n                    <jats:p>In this article, it is reported that syntheses of early signaling molecules such as PGE2 and NO can be used for the diagnosis and treatment target of periapical and periodontal infections.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s00784-024-05721-3","type":"journal-article","created":{"date-parts":[[2024,5,25]],"date-time":"2024-05-25T05:01:22Z","timestamp":1716613282000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["The importance of mechanosensitive cell mediated prostaglandin and nitric oxide synthesis in the pathogenesis of apical periodontitis: comparative with chronic periodontitis"],"prefix":"10.1007","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2439-3964","authenticated-orcid":false,"given":"Fatih","family":"Ozcelik","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0354-5108","authenticated-orcid":false,"given":"Seyda","family":"Ersahan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7159-1977","authenticated-orcid":false,"given":"Dursun Ali","family":"Sirin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1774-9689","authenticated-orcid":false,"given":"Ilbey Kayra","family":"Oz\u00e7elik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1844-1288","authenticated-orcid":false,"given":"Yelda Erdem","family":"Hepsenoglu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6757-4960","authenticated-orcid":false,"given":"Burak","family":"Karip","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,5,25]]},"reference":[{"key":"5721_CR1","unstructured":"Zhang S, Wu XY, Li YH (2001) [Bone adaptation and response to mechanical stress in bone]. 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