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Baseline GCF samples were quantitatively analysed using ELISA-based kits for levels of periodontopathogens (<jats:italic>Porphyromonas gingivalis<\/jats:italic> (<jats:italic>Pg<\/jats:italic>), <jats:italic>Aggregatibacter actinomycetemcomitans<\/jats:italic> (<jats:italic>Aa<\/jats:italic>), <jats:italic>Prevotella intermedia<\/jats:italic> (<jats:italic>Pi<\/jats:italic>), <jats:italic>Fusobacterium nucleatum<\/jats:italic> (<jats:italic>Fn<\/jats:italic>), <jats:italic>Treponema denticola<\/jats:italic> (<jats:italic>Td<\/jats:italic>), and <jats:italic>Tannerella forsythia<\/jats:italic> (<jats:italic>Tf<\/jats:italic>)) and associated with the primary therapy outcome using a \u201ctreat-to-target\u201d therapy endpoint (TE) defined as \u2264\u20094 sites with PD\u2009\u2265\u20095\u00a0mm six months after therapy.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>38.2% of the patients achieved TE. Patients failing to achieve TE revealed significantly increased levels of <jats:italic>Pg<\/jats:italic>, <jats:italic>Fn<\/jats:italic>, and <jats:italic>Tf<\/jats:italic> at baseline (<jats:italic>Pg<\/jats:italic>: <jats:italic>p<\/jats:italic>\u2009=\u20090.010, <jats:italic>Fn<\/jats:italic>: <jats:italic>p<\/jats:italic>\u2009=\u20090.008 <jats:italic>Tf<\/jats:italic>: <jats:italic>p<\/jats:italic>\u2009=\u20090.004). Multivariate binary logistic regression adjusted for sex, mean probing depth, diabetes, and current smoking status showed an independent relationship between <jats:italic>Tf<\/jats:italic> and the TE (aOR 2.570, <jats:italic>p<\/jats:italic>\u2009=\u20090.023).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>Increased microbial load is associated with decreased responsiveness to therapy. The findings suggest that specifically baseline <jats:italic>Tf<\/jats:italic> levels are associated with poorer treatment outcomes and might improve the accuracy of periodontal diagnosis.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Clinical relevance<\/jats:title>\n                <jats:p>The findings of this study support the concept of a critical biomass that is sufficient to induce and maintain an immune response within the periodontal pocket, which ultimately leads to irreversible tissue destruction. However, calculating this level in advance may serve as an early indicator for intervention.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Key finding<\/jats:title>\n                <jats:p>Baseline <jats:italic>Tannerella forsythia<\/jats:italic> levels are associated with poorer treatment outcome.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1007\/s00784-024-05904-y","type":"journal-article","created":{"date-parts":[[2024,9,13]],"date-time":"2024-09-13T06:02:24Z","timestamp":1726207344000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["The association between periodontal microbial biomarkers and primary therapy outcome"],"prefix":"10.1007","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8498-0736","authenticated-orcid":false,"given":"Nils","family":"Werner","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iris","family":"Frasheri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrin","family":"Heck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Ern","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Heym","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caspar Victor","family":"Bumm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Folwaczny","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,9,13]]},"reference":[{"key":"5904_CR1","doi-asserted-by":"publisher","unstructured":"Hajishengallis G, Chavakis T, Lambris JD (2020) Current understanding of periodontal disease pathogenesis and targets for host-modulation therapy. 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