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It was evaluated if there is an association in frequency to single periodontitis stages and grades.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Materials and methods<\/jats:title>\n            <jats:p>The study included 100 patients that were assigned to 4 test groups (periodontitis stages I-IV) and one healthy control group, each <jats:italic>n<\/jats:italic>\u2009=\u200920. Samples were collected from the deepest pockets of each sextant and analyzed for: <jats:italic>Fusobacterium nucleatum<\/jats:italic> (<jats:italic>F.n.<\/jats:italic>), <jats:italic>Aggregatibacter actinomycetemcomitans<\/jats:italic> (<jats:italic>A.a.<\/jats:italic>), <jats:italic>Porphyromonas gingivalis<\/jats:italic> (<jats:italic>P.g.<\/jats:italic>), <jats:italic>Tannerella forsythia<\/jats:italic> (<jats:italic>T.f.<\/jats:italic>), <jats:italic>Treponema denticola<\/jats:italic> (<jats:italic>T.d.<\/jats:italic>) and <jats:italic>Enterococcus faecalis<\/jats:italic> (<jats:italic>E.f.<\/jats:italic>).<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Among all samples, F.n. was most common (98%), followed by <jats:italic>T.f.<\/jats:italic> (73%), T.d. (66%), <jats:italic>P.g.<\/jats:italic> (45%), <jats:italic>A.a.<\/jats:italic> (12%) and <jats:italic>E.f.<\/jats:italic> (3%). Controls showed no <jats:italic>P.g.<\/jats:italic>, <jats:italic>A.a.<\/jats:italic> and <jats:italic>E.f.<\/jats:italic>. In stage I <jats:italic>T.f.<\/jats:italic> (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) and <jats:italic>T.d.<\/jats:italic> (<jats:italic>p<\/jats:italic>\u2009=\u20090.022) were significantly more frequent. In stages II and III, <jats:italic>P.g.<\/jats:italic>, T.f. and <jats:italic>T.d.<\/jats:italic> were significant (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090,001). In stage IV, <jats:italic>A.a.<\/jats:italic> (<jats:italic>p<\/jats:italic>\u2009=\u20090.003), <jats:italic>P.g<\/jats:italic>., <jats:italic>T.f.<\/jats:italic> and <jats:italic>T.d<\/jats:italic>. (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) occurred with significant higher frequency. Grade C was more common among stage III and IV (20%) as compared to stage I and II (2.5%). Patients diagnosed with stage IV and grade C, showed significant association to <jats:italic>A.a.<\/jats:italic> (<jats:italic>p<\/jats:italic>\u2009=\u20090.001) and <jats:italic>P.g.<\/jats:italic> (<jats:italic>p<\/jats:italic>\u2009=\u20090.021).<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>The present investigation proved significant correlation between periodontopathogenic key species, disease severity and progression risk. <jats:italic>A.a.<\/jats:italic> and <jats:italic>P.g<\/jats:italic> were most frequently in stage IV and grade C.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Clinical relevance<\/jats:title>\n            <jats:p>Microbial analysis provide additional information in regard to the classification of periodontitis.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1007\/s00784-025-06413-2","type":"journal-article","created":{"date-parts":[[2025,6,11]],"date-time":"2025-06-11T22:23:25Z","timestamp":1749680605000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Peridontopathogenic key species in correlation to the current classification system"],"prefix":"10.1007","volume":"29","author":[{"given":"Bernd","family":"Sigusch","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9591-0004","authenticated-orcid":false,"given":"Stefan","family":"Kranz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Heyder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Weber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9","family":"G\u00fcllmar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nargis","family":"Mahmudova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Reise","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,6,12]]},"reference":[{"key":"6413_CR1","doi-asserted-by":"publisher","first-page":"489","DOI":"10.1902\/jop.1992.63.6.489","volume":"63","author":"H L\u00f6e","year":"1992","unstructured":"L\u00f6e H, Anerud A, Boysen H (1992) The natural history of periodontal disease in man: prevalence, severity, and extent of gingival recession. 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