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In vitro, ex vivo, and clinical studies were considered eligible. Studies focusing exclusively on fluorescence-based caries detection without an excavation component were excluded. Two reviewers independently screened titles, abstracts, and full texts according to predefined eligibility criteria.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A total of 16 studies were included, predominantly laboratory-based (12 in vitro, 1 ex vivo), with only three clinical studies. Across the included studies, fluorescence-guided excavation generally demonstrated enhanced selectivity, enabling preservation of less mineralized dentin while maintaining effective removal of bacterially infected tissue, although these advantages were not observed uniformly across all studies. Microbiological analyses showed reduced residual bacterial contamination following FACE compared with conventional excavation and dye-assisted methods. In addition, fluorescence signals were found to correlate with bacterial presence and diversity, supporting their biological relevance. Clinical studies indicated improved intraoperative detection of residual caries and provided biologically relevant information on bacterial contamination. However, the evidence base was heterogeneous and primarily focused on surrogate outcomes, with limited data on long-term clinical performance.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Fluorescence-guided caries excavation represents a promising adjunctive approach that provides qualitative intraoperative visualization of fluorescence characteristics associated with bacterial contamination, rather than a validated quantitative threshold for tissue removal. While the available evidence suggests improved excavation selectivity and microbiological outcomes, robust clinical data remain limited. Further well-designed clinical studies are required to determine the impact of FACE on patient-centered outcomes and its role in routine operative dentistry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Clinical relevance<\/jats:title>\n                    <jats:p>Fluorescence-guided technologies may assist clinicians in identifying bacterially infected dentin during cavity preparation and facilitate selective caries removal, potentially improving intraoperative decision-making.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s00784-026-07119-9","type":"journal-article","created":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T09:30:02Z","timestamp":1789032602000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Fluorescence-aided caries excavation in minimally invasive dentistry: a scoping review"],"prefix":"10.1007","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3953-9145","authenticated-orcid":false,"given":"Marco M.","family":"Herz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes","family":"\u00d6sterreicher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Meller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,10]]},"reference":[{"key":"7119_CR1","doi-asserted-by":"crossref","unstructured":"Frencken JE, Peters MC, Manton DJ (2012) Minimal intervention dentistry for managing dental caries. 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