{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T18:33:58Z","timestamp":1790534038005,"version":"4.1.0"},"reference-count":45,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2018,6,5]],"date-time":"2018-06-05T00:00:00Z","timestamp":1528156800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Extremophiles"],"published-print":{"date-parts":[[2018,9]]},"DOI":"10.1007\/s00792-018-1035-6","type":"journal-article","created":{"date-parts":[[2018,6,5]],"date-time":"2018-06-05T07:15:46Z","timestamp":1528182946000},"page":"751-759","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Life from the ashes: survival of dry bacterial spores after very high temperature exposure"],"prefix":"10.1007","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5755-7235","authenticated-orcid":false,"given":"Lynda","family":"Beladjal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom","family":"Gheysens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James S.","family":"Clegg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed","family":"Amar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johan","family":"Mertens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2018,6,5]]},"reference":[{"key":"1035_CR1","doi-asserted-by":"publisher","first-page":"443","DOI":"10.1590\/S1517-838246220140219","volume":"46","author":"T Aanniz","year":"2015","unstructured":"Aanniz T, Ouadghiri M, Melloul M, Swings J, Elfahime E, Ibijbijen J, Ismaili M, Amar M (2015) Thermophilic bacteria in Moroccan hot springs, salt marshes and desert soils. Braz J Microbiol 46:443\u2013453","journal-title":"Braz J Microbiol"},{"key":"1035_CR2","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1016\/j.mimet.2009.09.026","volume":"79","author":"A Adiguzel","year":"2009","unstructured":"Adiguzel A, Ozkan H, Baris O, Inan K, Gulluce M, Sahin F (2009) Identification and characterization of thermophilic bacteria isolated from hot springs in Turkey. J Microbiol Methods 79:321\u2013328","journal-title":"J Microbiol Methods"},{"key":"1035_CR3","doi-asserted-by":"publisher","first-page":"6759","DOI":"10.1128\/jb.178.23.6759-6765.1996","volume":"178","author":"HJ Agostini","year":"1996","unstructured":"Agostini HJ, Carroll JD, Minton KW (1996) Identification and characterization of gvrA, a DNA repair gene of Deinococcus radiodurans. J Bacteriol 178:6759\u20136765","journal-title":"J Bacteriol"},{"key":"1035_CR4","doi-asserted-by":"publisher","first-page":"162","DOI":"10.1016\/j.dnarep.2012.12.005","volume":"12","author":"CH Alonso","year":"2013","unstructured":"Alonso CH, Cardenas PP, Sanchez H, Hejna J, Suzuki Y, Takeyasu K (2013) Early steps of double-strand break repair in Bacillus subtilis. DNA Repair 12:162\u2013176","journal-title":"DNA Repair"},{"key":"1035_CR5","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","volume":"215","author":"SF Altschul","year":"1990","unstructured":"Altschul SF, Gish W, Miller W, Meyers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403\u2013410","journal-title":"J Mol Biol"},{"key":"1035_CR6","volume-title":"Textbook of microbiology","author":"R Ananthanarayan","year":"2006","unstructured":"Ananthanarayan R, Paniker CKJ (2006) Textbook of microbiology, 7th edn. Orient Longman Private Ltd., Chennai","edition":"7"},{"key":"1035_CR7","doi-asserted-by":"publisher","first-page":"594","DOI":"10.1016\/j.ifset.2011.06.008","volume":"12","author":"T Anthierens","year":"2011","unstructured":"Anthierens T, Ragaert P, Verbrugge S, Ouchchen A, De Geest BG, Noseda B, Mertens J, Beladjal L, De Cuyper D, Dierickx W, Du Prez F, Devlieghere F (2011) Use of endospore-forming bacteria as an active oxygen scavenger in plastic packaging materials. Innov Food Sci Emerg Technol 12:594\u2013599","journal-title":"Innov Food Sci Emerg Technol"},{"key":"1035_CR8","doi-asserted-by":"publisher","first-page":"1315","DOI":"10.1099\/ijs.0.02473-0","volume":"53","author":"AO Belduz","year":"2003","unstructured":"Belduz AO, Dulger S, Demirbag Z (2003) Anoxybacillus gonensis sp. nov., a moderately thermophilic, xylose-utilizing, endospore-forming bacterium. Int J Syst Evol Microbiol 53:1315\u20131320","journal-title":"Int J Syst Evol Microbiol"},{"key":"1035_CR9","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1093\/nar\/27.1.12","volume":"27","author":"DA Benson","year":"1999","unstructured":"Benson DA, Boguski MS, Lipman DJ, Ostell J, Ouellette BFF, Rapp BA, Wheeler DL (1999) GenBank. Nucleic Acids Res 27:12\u201317","journal-title":"Nucleic Acids Res"},{"key":"1035_CR10","doi-asserted-by":"publisher","first-page":"1513","DOI":"10.1093\/nar\/7.6.1513","volume":"7","author":"H Birnboim","year":"1979","unstructured":"Birnboim H, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7:1513\u20131523","journal-title":"Nucleic Acids Res"},{"key":"1035_CR11","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1128\/AEM.29.6.859-860.1975","volume":"29","author":"WW Bond","year":"1975","unstructured":"Bond WW, Favero MS (1975) Thermal profile of a Bacillus species (ATCC 27380) extremely resistant to dry heat. Appl Microbiol 29:859","journal-title":"Appl Microbiol"},{"key":"1035_CR12","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1099\/00207713-27-2-157","volume":"27","author":"WW Bond","year":"1977","unstructured":"Bond WW, Favero MS (1977) Bacillus xerothermodurans sp. nov., a species forming endospores extremely resistant to dry heat. Int J Syst Bacteriol 27:157\u2013160","journal-title":"Int J Syst Bacteriol"},{"key":"1035_CR13","doi-asserted-by":"publisher","first-page":"26922","DOI":"10.1074\/jbc.M602297200","volume":"281","author":"A Chandor","year":"2006","unstructured":"Chandor A, Berteau O, Douki T, Gasparutto D, Sanakis Y, Ollagnier-de-Choudens S, Atta M, Fontecave M (2006) Dinucleotide spore photoproduct, a minimal substrate of the DNA repair spore photoproduct lyase enzyme from Bacillus subtilis. J Biol Chem 281:26922\u201326931","journal-title":"J Biol Chem"},{"key":"1035_CR14","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1023\/A:1026555830014","volume":"78","author":"J Chun","year":"2000","unstructured":"Chun J, Bae KS (2000) Phylogenetic analysis of Bacillus subtilis and related taxa based on partial gyrA gene sequences. Antonie Van Leeuwenhoek 78:123\u2013127","journal-title":"Antonie Van Leeuwenhoek"},{"issue":"2\u2013104","key":"1035_CR15","first-page":"102","volume":"22","author":"L Ciera","year":"2014","unstructured":"Ciera L, Beladjal L, Almeras X, Gheysens T, Nierstrasz V, Van Langenhove L, Mertens J (2014a) Resistance of Bacillus amyloliquefaciens spores to melt extrusion process conditions. Fibres Text East Eur 22(2\u2013104):102\u2013107","journal-title":"Fibres Text East Eur"},{"issue":"4\u2013106","key":"1035_CR16","first-page":"29","volume":"22","author":"L Ciera","year":"2014","unstructured":"Ciera L, Beladjal L, Almeras X, Gheysens T, Van Landuyt L, Mertens J, Nierstrasz V, Van Langenhove L (2014b) Morphological and material properties of polyethylene terephthalate (PET) fibres with spores incorporated. Fibres Text East Eur 22(4\u2013106):29\u201336","journal-title":"Fibres Text East Eur"},{"key":"1035_CR17","doi-asserted-by":"publisher","first-page":"1600","DOI":"10.1111\/j.1365-2672.2008.04127.x","volume":"106","author":"W Coleman","year":"2009","unstructured":"Coleman W, Setlow P (2009) Analysis of damage due to moist heat treatment of spores of Bacillus subtilis. J Appl Microbiol 106:1600\u20131607","journal-title":"J Appl Microbiol"},{"key":"1035_CR18","doi-asserted-by":"publisher","first-page":"8458","DOI":"10.1128\/JB.01242-07","volume":"189","author":"WH Coleman","year":"2007","unstructured":"Coleman WH, Chen D, Li Y, Cowan AE, Setlow P (2007) How moist heat kills spores of Bacillus subtilis. J Bacteriol 189:8458\u20138466","journal-title":"J Bacteriol"},{"key":"1035_CR19","doi-asserted-by":"publisher","first-page":"317","DOI":"10.1128\/AEM.67.1.317-322.2001","volume":"67","author":"L Coroller","year":"2001","unstructured":"Coroller L, Legu\u00e9rinel I, Mafart P (2001) Effect of water activities of heating and recovery media on apparent heat resistance of Bacillus cereus spores. Appl Environ Microbiol 67:317\u2013322","journal-title":"Appl Environ Microbiol"},{"key":"1035_CR20","doi-asserted-by":"publisher","first-page":"606","DOI":"10.1111\/j.1365-2672.2004.02495.x","volume":"98","author":"DE Cortezzo","year":"2005","unstructured":"Cortezzo DE, Setlow P (2005) Analysis of factors that influence the sensitivity of spores of Bacillus subtilis to DNA damaging chemicals. J Appl Microbiol 98:606\u2013617","journal-title":"J Appl Microbiol"},{"key":"1035_CR21","doi-asserted-by":"publisher","first-page":"1499","DOI":"10.1099\/ijs.0.02863-0","volume":"54","author":"S Dulger","year":"2004","unstructured":"Dulger S, Demirbag Z, Belduz AO (2004) Anoxybacillus ayderensis sp. nov. and Anoxybacillus kestanbolensis sp. nov. Inter J Syst Evol Microbiol 54:1499\u20131503","journal-title":"Inter J Syst Evol Microbiol"},{"key":"1035_CR22","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1016\/S0027-5107(02)00093-3","volume":"503","author":"LDCH Espitia","year":"2002","unstructured":"Espitia LDCH, Caley C, Bagyan I, Setlow P (2002) Base-change mutations induced by various treatments of Bacillus subtilis spores with and without DNA protective small, acid-soluble spore proteins. Mutat Res Fund Mol Mech Mutagen 503:77\u201384","journal-title":"Mutat Res Fund Mol Mech Mutagen"},{"key":"1035_CR23","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1111\/j.1574-6968.2001.tb10921.x","volume":"205","author":"D Gevers","year":"2001","unstructured":"Gevers D, Huys G, Swings J (2001) Applicability of rep-PCR fingerprinting for identification of Lactobacillus species. FEMS Microbiol Lett 205:31\u201336","journal-title":"FEMS Microbiol Lett"},{"key":"1035_CR24","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1111\/j.1365-2672.1980.tb01222.x","volume":"48","author":"TR Gurney","year":"1980","unstructured":"Gurney TR, Quesnel LB (1980) Thermal activation and dry-heat inactivation of spores of Bacillus subtilis MD2 and Bacillus subtilis var. niger. J Appl Bacteriol 48:231\u2013247","journal-title":"J Appl Bacteriol"},{"key":"1035_CR25","volume-title":"Laboratory exercises in microbiology","author":"JP Harley","year":"2002","unstructured":"Harley JP, Prescott LM (2002) Laboratory exercises in microbiology, 5th edn. The McGraw-Hill Compagnies, New York","edition":"5"},{"key":"1035_CR26","doi-asserted-by":"publisher","first-page":"298","DOI":"10.1089\/dna.2013.2056","volume":"32","author":"M Karni","year":"2013","unstructured":"Karni M, Zidon D, Polak P, Zalevsky Z, Shefi O (2013) Thermal degradation of DNA. DNA Cell Biol 32:298\u2013301","journal-title":"DNA Cell Biol"},{"key":"1035_CR27","doi-asserted-by":"publisher","first-page":"3015","DOI":"10.1128\/AEM.69.5.3015-3019.2003","volume":"69","author":"C Laroche","year":"2003","unstructured":"Laroche C, Gervais P (2003) Unexpected thermal destruction of dried, glass bead-immobilized microorganisms as a function of water activity. Appl Environ Microbiol 69:3015\u20133019","journal-title":"Appl Environ Microbiol"},{"key":"1035_CR28","first-page":"633","volume":"16","author":"S Lee","year":"2006","unstructured":"Lee S, Sim S (2006) Increased heat resistance of Geobacillus stearothermophilus spores heat-shocked during sporulation. J Microbiol Biotechnol 16:633\u2013636","journal-title":"J Microbiol Biotechnol"},{"key":"1035_CR29","doi-asserted-by":"publisher","first-page":"530","DOI":"10.1128\/MMBR.05020-11","volume":"76","author":"JS Lenhart","year":"2012","unstructured":"Lenhart JS, Schroeder JW, Walsh BW, Simmons LA (2012) DNA repair and genome maintenance in Bacillus subtilis. Microbiol Mol Biol Rev 76:530\u2013564","journal-title":"Microbiol Mol Biol Rev"},{"key":"1035_CR30","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1016\/j.resmic.2015.08.006","volume":"167","author":"JS Lenhart","year":"2016","unstructured":"Lenhart JS, Pillon MC, Guarn\u00e9 A, Biteen J, Simmons LA (2016) Mismatch repair in Gram-positive bacteria. Res Microbiol 167:4\u201312","journal-title":"Res Microbiol"},{"key":"1035_CR31","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1016\/j.dnarep.2017.09.007","volume":"59","author":"E Markkanen","year":"2017","unstructured":"Markkanen E (2017) Not breathing is not an option: how to deal with oxidative DNA damage. DNA Repair 59:82\u2013105","journal-title":"DNA Repair"},{"key":"1035_CR32","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1111\/j.1095-8312.2007.00902.x","volume":"93","author":"J Mertens","year":"2008","unstructured":"Mertens J, Beladjal L, Alcantara A, Fougnies L, Van Der Straeten D, Clegg J (2008) Survival of dried eukaryotes (anhydrobiotes) after exposure to very high temperatures. Biol J Linn Soc 93:15\u201322","journal-title":"Biol J Linn Soc"},{"key":"1035_CR33","doi-asserted-by":"publisher","first-page":"967","DOI":"10.1016\/j.dnarep.2007.02.016","volume":"6","author":"B Michel","year":"2007","unstructured":"Michel B, Boubakri H, Baharoglu Z, LeMasson M, Lestini R (2007) Recombination proteins and rescue of arrested replication forks. DNA Repair 6:967\u2013980","journal-title":"DNA Repair"},{"key":"1035_CR34","doi-asserted-by":"publisher","first-page":"548","DOI":"10.1128\/MMBR.64.3.548-572.2000","volume":"64","author":"WL Nicholson","year":"2000","unstructured":"Nicholson WL, Munakata N, Horneck G, Melosh HJ, Setlow P (2000) Resistance of Bacillus endospores to extreme terrestrial and extraterrestrial environments. Microbiol Mol Biol Rev 64:548\u2013572","journal-title":"Microbiol Mol Biol Rev"},{"key":"1035_CR35","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1111\/j.1472-765X.1989.tb00262.x","volume":"8","author":"D Pitcher","year":"1989","unstructured":"Pitcher D, Saunders N, Owen R (1989) Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8:151\u2013156","journal-title":"Lett Appl Microbiol"},{"key":"1035_CR36","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1111\/j.1574-6941.2008.00638.x","volume":"67","author":"N Rameshkumar","year":"2009","unstructured":"Rameshkumar N, Nair S (2009) Isolation and molecular characterization of genetically diverse antagonistic, diazotrophic red-pigmented vibrios from different mangrove rhizospheres. FEMS Microbiol Ecol 67:455\u2013467","journal-title":"FEMS Microbiol Ecol"},{"key":"1035_CR37","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1111\/j.1439-0434.2007.01329.x","volume":"156","author":"N Sadfi-Zouaoui","year":"2008","unstructured":"Sadfi-Zouaoui N, Essghaier B, Hajlaoui MR, Fardeau ML, Cayaol JL, Ollivier B, Boudabous A (2008) Ability of moderately halophilic bacteria to control grey mould disease on tomato fruits. J Phytopathol 156:42\u201352","journal-title":"J Phytopathol"},{"key":"1035_CR38","doi-asserted-by":"publisher","first-page":"550","DOI":"10.1016\/j.mib.2003.10.001","volume":"6","author":"P Setlow","year":"2003","unstructured":"Setlow P (2003) Spore germination. Curr Opin Microbiol 6:550\u2013556","journal-title":"Curr Opin Microbiol"},{"key":"1035_CR39","doi-asserted-by":"publisher","first-page":"514","DOI":"10.1111\/j.1365-2672.2005.02736.x","volume":"101","author":"P Setlow","year":"2006","unstructured":"Setlow P (2006) Spores of Bacillus subtilis: their resistance to and killing by radiation, heat and chemicals. J Appl Microbiol 101:514\u2013525","journal-title":"J Appl Microbiol"},{"issue":"5","key":"1035_CR40","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1128\/microbiolspec.TBS-0003-2012","volume":"2","author":"P Setlow","year":"2014","unstructured":"Setlow P (2014) Spore resistance properties. Microbiol Spect 2(5):201\u2013215","journal-title":"Microbiol Spect"},{"key":"1035_CR41","doi-asserted-by":"crossref","first-page":"2787","DOI":"10.1128\/AEM.61.7.2787-2790.1995","volume":"61","author":"B Setlow","year":"1995","unstructured":"Setlow B, Setlow P (1995) Small, acid-soluble proteins bound to DNA protect Bacillus subtilis spores from killing by dry heat. Appl Environ Microbiol 61:2787\u20132790","journal-title":"Appl Environ Microbiol"},{"issue":"12","key":"1035_CR42","doi-asserted-by":"publisher","first-page":"3486","DOI":"10.1128\/jb.178.12.3486-3495.1996","volume":"178","author":"B Setlow","year":"1996","unstructured":"Setlow B, Setlow P (1996) Role of DNA repair in Bacillus subtilis spore resistance. J Bacteriol 178(12):3486\u20133495","journal-title":"J Bacteriol"},{"key":"1035_CR43","doi-asserted-by":"publisher","first-page":"805","DOI":"10.1111\/jam.12421","volume":"116","author":"B Setlow","year":"2014","unstructured":"Setlow B, Parish S, Zhang P, Li YQ, Neely WC, Setlow P (2014) Mechanism of killing of spores of Bacillus anthracis in a high-temperature gas environment, and analysis of DNA damage generated by various decontamination treatments of spores of Bacillus anthracis, Bacillus subtilis and Bacillus thuringiensis. J Appl Microbiol 116:805\u2013814","journal-title":"J Appl Microbiol"},{"key":"1035_CR44","first-page":"25","volume":"5","author":"J Versalovic","year":"1994","unstructured":"Versalovic J, Schneider M, de Bruijn FJ, Lupski JR (1994) Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction. Methods Mol Cell Biol 5:25\u201340","journal-title":"Methods Mol Cell Biol"},{"key":"1035_CR45","doi-asserted-by":"publisher","first-page":"578","DOI":"10.1016\/j.cej.2015.05.021","volume":"279","author":"W Zhou","year":"2015","unstructured":"Zhou W, Orr MW, Jian G, Watt SK, Lee VT, Zachariah MR (2015) Inactivation of bacterial spores subjected to sub-second thermal stress. Chem Eng J 279:578\u2013588","journal-title":"Chem Eng J"}],"container-title":["Extremophiles"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s00792-018-1035-6\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00792-018-1035-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s00792-018-1035-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,7]],"date-time":"2024-07-07T08:22:04Z","timestamp":1720340524000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s00792-018-1035-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,6,5]]},"references-count":45,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2018,9]]}},"alternative-id":["1035"],"URL":"https:\/\/doi.org\/10.1007\/s00792-018-1035-6","relation":{},"ISSN":["1431-0651","1433-4909"],"issn-type":[{"value":"1431-0651","type":"print"},{"value":"1433-4909","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,6,5]]},"assertion":[{"value":"12 January 2018","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"31 May 2018","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 June 2018","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}]}}