{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T06:59:18Z","timestamp":1773298758242,"version":"3.50.1"},"reference-count":19,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2021,7,21]],"date-time":"2021-07-21T00:00:00Z","timestamp":1626825600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2021,7,21]],"date-time":"2021-07-21T00:00:00Z","timestamp":1626825600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Med Mol Morphol"],"published-print":{"date-parts":[[2021,12]]},"DOI":"10.1007\/s00795-021-00298-2","type":"journal-article","created":{"date-parts":[[2021,7,21]],"date-time":"2021-07-21T08:03:06Z","timestamp":1626854586000},"page":"374-379","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Next-generation sequencing of 16S rRNA for identification of invasive bacterial pathogens in a formalin-fixed paraffin-embedded placental specimen: a case report of perinatal fulminant Streptococcus pyogenes infection"],"prefix":"10.1007","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7110-980X","authenticated-orcid":false,"given":"Tasuku","family":"Mariya","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toyotaka","family":"Sato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuya","family":"Fujibe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manami","family":"Ishido","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Shimada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Terufumi","family":"Kubo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoko","family":"Nagai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wataru","family":"Arai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suguru E.","family":"Tanaka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyota","family":"Ashikawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiyuki","family":"Sakuraba","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinichi","family":"Ishioka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shin-ichi","family":"Yokota","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsuyoshi","family":"Saito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,7,21]]},"reference":[{"key":"298_CR1","doi-asserted-by":"publisher","first-page":"727","DOI":"10.1016\/j.jiac.2016.06.013","volume":"22","author":"Y Tanaka","year":"2016","unstructured":"Tanaka Y, Gotoh K, Teramachi M, Ishimoto K, Tsumura N, Shindou S, Yamashita Y (2016) Molecular epidemiology, antimicrobial susceptibility, and characterization of macrolide-resistant Streptococcus pyogenes in Japan. J Infect Chemother 22:727\u2013732","journal-title":"J Infect Chemother"},{"key":"298_CR2","doi-asserted-by":"publisher","first-page":"88","DOI":"10.1186\/s13613-018-0438-y","volume":"8","author":"M Schmitz","year":"2018","unstructured":"Schmitz M, Roux X, Huttner B, Pugin J (2018) Streptococcal toxic shock syndrome in the intensive care unit. Ann Intensive Care 8:88","journal-title":"Ann Intensive Care"},{"key":"298_CR3","first-page":"153","volume":"94","author":"H Udagawa","year":"1999","unstructured":"Udagawa H, Oshio Y, Shimizu Y (1999) Serious group A streptococcal infection around delivery. Obstet Gynecol 94:153\u2013157","journal-title":"Obstet Gynecol"},{"key":"298_CR4","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1016\/j.jiac.2018.10.004","volume":"25","author":"H Tanaka","year":"2019","unstructured":"Tanaka H, Katsuragi S, Hasegawa J, Tanaka K, Osato K, Nakata M, Murakoshi T, Sekizawa A, Kanayama N, Ishiwata I, Ikeda T (2019) The most common causative bacteria in maternal sepsis-related deaths in Japan were group A Streptococcus: a nationwide survey. J Infect Chemother 25:41\u201344","journal-title":"J Infect Chemother"},{"key":"298_CR5","doi-asserted-by":"publisher","first-page":"928","DOI":"10.1111\/jog.14619","volume":"47","author":"E Hayata","year":"2021","unstructured":"Hayata E, Nakata M, Hasegawa J, Tanaka H, Murakoshi T, Mitsuda N, Sekizawa A, Ikeda T, Ishiwata I (2021) Nationwide study of mortality and survival in pregnancy-related streptococcal toxic shock syndrome. J Obstet Gynaecol Res 47:928\u2013934","journal-title":"J Obstet Gynaecol Res"},{"key":"298_CR6","doi-asserted-by":"publisher","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","volume":"30","author":"AM Bolger","year":"2014","unstructured":"Bolger AM, Lohse M, Usadel B (2014) Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics 30:2114\u20132120","journal-title":"Bioinformatics"},{"key":"298_CR7","doi-asserted-by":"publisher","first-page":"1","DOI":"10.2174\/1875036201307010001","volume":"7","author":"E Aronesty","year":"2013","unstructured":"Aronesty E (2013) Comparison of sequencing utility programs. Open Bioinform J 7:1\u20138","journal-title":"Open Bioinform J"},{"key":"298_CR8","doi-asserted-by":"publisher","first-page":"863","DOI":"10.1093\/bioinformatics\/btr026","volume":"27","author":"R Schmieder","year":"2011","unstructured":"Schmieder R, Edwards R (2011) Quality control and preprocessing of metagenomic datasets. Bioinformatics 27:863\u2013864","journal-title":"Bioinformatics"},{"key":"298_CR9","doi-asserted-by":"publisher","first-page":"335","DOI":"10.1038\/nmeth.f.303","volume":"7","author":"JG Caporaso","year":"2010","unstructured":"Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, Fierer N, Pe\u00f1a AG, Goodrich JK, Gordon JI, Huttley GA, Kelley ST, Knights D, Koenig JE, Ley RE, Lozupone CA, McDonald D, Muegge BD, Pirrung M, Reeder J, Sevinsky JR, Turnbaugh PJ, Walters WA, Widmann J, Yatsunenko T, Zaneveld J, Knight R (2010) QIIME allows analysis of high-throughput community sequencing data. Nat Methods 7:335\u2013336","journal-title":"Nat Methods"},{"key":"298_CR10","doi-asserted-by":"publisher","first-page":"D590","DOI":"10.1093\/nar\/gks1219","volume":"41","author":"C Quast","year":"2013","unstructured":"Quast C, Pruesse E, Yilmaz P, Gerken J, Schweer T, Yarza P, Peplies J, Gl\u00f6ckner FO (2013) The SILVA ribosomal RNA gene database project: improved data processing and web-based tools. Nucleic Acids Res 41:D590\u2013D596","journal-title":"Nucleic Acids Res"},{"issue":"Suppl 8","key":"298_CR11","doi-asserted-by":"publisher","first-page":"S17","DOI":"10.1186\/1471-2164-13-S8-S17","volume":"13","author":"JM Fettweis","year":"2012","unstructured":"Fettweis JM, Serrano MG, Sheth NU, Mayer CM, Glascock AL, Brooks JP, Jefferson KK, Buck GA (2012) Species-level classification of the vaginal microbiome. BMC Genomics 13(Suppl 8):S17","journal-title":"BMC Genomics"},{"key":"298_CR12","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1155\/S1064744900000302","volume":"8","author":"PB Mead","year":"2000","unstructured":"Mead PB, Winn WC (2000) Vaginal-rectal colonization with group A streptococci in late pregnancy. Infect Dis Obstet Gynecol 8:217\u2013219","journal-title":"Infect Dis Obstet Gynecol"},{"key":"298_CR13","doi-asserted-by":"publisher","first-page":"329","DOI":"10.1038\/s41586-019-1451-5","volume":"572","author":"MC de Goffau","year":"2019","unstructured":"de Goffau MC, Lager S, Sovio U, Gaccioli F, Cook E, Peacock SJ, Parkhill J, Charnock-Jones DS, Smith GCS (2019) Human placenta has no microbiome but can contain potential pathogens. Nature 572:329\u2013334","journal-title":"Nature"},{"key":"298_CR14","doi-asserted-by":"publisher","first-page":"293","DOI":"10.1056\/NEJM199207303270501","volume":"327","author":"DA Relman","year":"1992","unstructured":"Relman DA, Schmidt TM, MacDermott RP, Falkow S (1992) Identification of the uncultured bacillus of Whipple\u2019s disease. N Engl J Med 327:293\u2013301","journal-title":"N Engl J Med"},{"key":"298_CR15","doi-asserted-by":"publisher","first-page":"132","DOI":"10.1016\/j.humpath.2016.09.015","volume":"59","author":"LD Racsa","year":"2017","unstructured":"Racsa LD, DeLeon-Carnes M, Hiskey M, Guarner J (2017) Identification of bacterial pathogens from formalin-fixed, paraffin-embedded tissues by using 16S sequencing: retrospective correlation of results to clinicians\u2019 responses. Hum Pathol 59:132\u2013138","journal-title":"Hum Pathol"},{"key":"298_CR16","doi-asserted-by":"publisher","first-page":"431","DOI":"10.1093\/ajcp\/aqz055","volume":"152","author":"IH Solomon","year":"2019","unstructured":"Solomon IH, Lin C, Horback KL, Kanjilal S, Rojas-Rudilla V, Brigl M, Laga AC, Lindeman NI, Padera RF (2019) Utility of histologic and histochemical screening for 16S ribosomal RNA gene sequencing of formalin-fixed, paraffin-embedded tissue for bacterial endocarditis. Am J Clin Pathol 152:431\u2013437","journal-title":"Am J Clin Pathol"},{"key":"298_CR17","doi-asserted-by":"publisher","first-page":"e01834","DOI":"10.1128\/JCM.01834-18","volume":"57","author":"BG Nezami","year":"2019","unstructured":"Nezami BG, Jani M, Alouani D, Rhoads DD, Sadri N (2019) Helicobacter pylori mutations detected by next-generation sequencing in formalin-fixed, paraffin-embedded gastric biopsy specimens are associated with treatment failure. J Clin Microbiol 57:e01834-e1918","journal-title":"J Clin Microbiol"},{"key":"298_CR18","doi-asserted-by":"publisher","first-page":"1287","DOI":"10.1016\/j.jmoldx.2020.06.017","volume":"22","author":"PMK Larkin","year":"2020","unstructured":"Larkin PMK, Lawson KL, Contreras DA, Le CQ, Trejo M, Realegeno S, Hilt EE, Chandrasekaran S, Garner OB, Fishbein GA, Yang S (2020) Amplicon-based next-generation sequencing for detection of fungi in formalin-fixed, paraffin-embedded tissues: correlation with histopathology and clinical applications. J Mol Diagn 22:1287\u20131293","journal-title":"J Mol Diagn"},{"key":"298_CR19","doi-asserted-by":"publisher","first-page":"780","DOI":"10.1016\/j.acthis.2018.09.005","volume":"120","author":"K Vitosevic","year":"2018","unstructured":"Vitosevic K, Todorovic M, Varljen T, Slovic Z, Matic S, Todorovic D (2018) Effect of formalin fixation on pcr amplification of DNA isolated from healthy autopsy tissues. Acta Histochem 120:780\u2013788","journal-title":"Acta Histochem"}],"container-title":["Medical Molecular Morphology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00795-021-00298-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s00795-021-00298-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s00795-021-00298-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,30]],"date-time":"2021-10-30T16:04:33Z","timestamp":1635609873000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s00795-021-00298-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7,21]]},"references-count":19,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["298"],"URL":"https:\/\/doi.org\/10.1007\/s00795-021-00298-2","relation":{},"ISSN":["1860-1480","1860-1499"],"issn-type":[{"value":"1860-1480","type":"print"},{"value":"1860-1499","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,7,21]]},"assertion":[{"value":"23 June 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 July 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 July 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare that they have no conflict of interest. All authors have contributed significantly to the research described in the paper and have reviewed the manuscript and agree with its contents and with the authorship listed.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"Written informed consent was obtained from the patient according to the guidelines of the Declaration of Helsinki. This investigation of perinatal infection was approved by the Ethics Committee of Sapporo Medical University of Medicine.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval and consent to participate"}}]}}