{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T04:32:09Z","timestamp":1741753929327,"version":"3.38.0"},"reference-count":35,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2004,5,1]],"date-time":"2004-05-01T00:00:00Z","timestamp":1083369600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatr Dev Pathol"],"published-print":{"date-parts":[[2004,5]]},"abstract":"<jats:p> Somatic mutation (SM) analysis provides a useful tool for understanding the stages at which neoplastic differentiate from normal B-cells. B-cell precursor neoplasms are considered to be somatically premutational. However, the variable frequency of SM of the variable region (VH) genes has been described in cases of precursor B-cell acute lymphoblastic leukemia (PB-ALL). To better characterize PB-ALL based on the differentiation stage, we investigated the SM of the VH genes expressed by tumor cells of the surface immunoglobulin (sIg)<jats:sup>-<\/jats:sup> HBL-3 cell line derived from childhood PB-ALL. In the HBL-3 cell line, the rearranged Ig heavy chain VH gene sequence showed no SM in the complementarity-determining regions of 1, 2, and 3, or in the framework regions of 1, 2, and 3 relative to the putative germline VH gene sequences. In addition, the VH segment of HBL-3 cells showed no intraclonal sequence heterogeneity, indicating ongoing SM. Our data demonstrated that HBL-3 cells express unmutated and developmentally regulated rearrangement of VH genes at the stage of B-cell precursor cells. HBL-3 cells, which are derived only from the sIg<jats:sup>-<\/jats:sup> PB-ALL, showed that SM cannot be recognized in VH genes of tumor cells before the expression of sIg. <\/jats:p>","DOI":"10.1007\/s10024-003-8085-0","type":"journal-article","created":{"date-parts":[[2004,3,19]],"date-time":"2004-03-19T18:57:37Z","timestamp":1079722657000},"page":"250-257","source":"Crossref","is-referenced-by-count":0,"title":["HBL-3 Cell Line, Derived from Precursor B-cell Lymphoblastic Leukemia, Lacks Somatic Hypermutation of Immunoglobulin Heavy Chain Variable Region Genes"],"prefix":"10.1177","volume":"7","author":[{"given":"Hiroshi","family":"Hojo","sequence":"first","affiliation":[{"name":"First Department of Pathology, Fukushima Medical University School of Medicine, 1 Hikariga-oka, Fukushima, 960-1295, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshikazu","family":"Sasaki","sequence":"additional","affiliation":[{"name":"First Department of Pathology, Fukushima Medical University School of Medicine, 1 Hikariga-oka, Fukushima, 960-1295, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoya","family":"Nakamura","sequence":"additional","affiliation":[{"name":"First Department of Pathology, Fukushima Medical University School of Medicine, 1 Hikariga-oka, Fukushima, 960-1295, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michiko","family":"Sato","sequence":"additional","affiliation":[{"name":"First Department of Pathology, Fukushima Medical University School of Medicine, 1 Hikariga-oka, Fukushima, 960-1295, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masafumi","family":"Abe","sequence":"additional","affiliation":[{"name":"First Department of Pathology, Fukushima Medical University School of Medicine, 1 Hikariga-oka, Fukushima, 960-1295, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2004,5,1]]},"reference":[{"key":"bibr1-s10024-003-8085-0","first-page":"111","volume-title":"World Health Organization Classification of Tumors. Pathology & Genetics: Tumors of Hematopoietic and Lymphoid Tumors","author":"Brunning RD","year":"2001"},{"doi-asserted-by":"publisher","key":"bibr2-s10024-003-8085-0","DOI":"10.1038\/302575a0"},{"doi-asserted-by":"publisher","key":"bibr3-s10024-003-8085-0","DOI":"10.1111\/j.1600-065X.1986.tb01484.x"},{"doi-asserted-by":"publisher","key":"bibr4-s10024-003-8085-0","DOI":"10.1038\/342929a0"},{"doi-asserted-by":"publisher","key":"bibr5-s10024-003-8085-0","DOI":"10.1038\/354389a0"},{"key":"bibr6-s10024-003-8085-0","first-page":"3","volume":"97","author":"Hentges F.","year":"1994","journal-title":"Clin Exp Immunol"},{"doi-asserted-by":"publisher","key":"bibr7-s10024-003-8085-0","DOI":"10.1056\/NEJM199911113412007"},{"doi-asserted-by":"publisher","key":"bibr8-s10024-003-8085-0","DOI":"10.1309\/85CJ-U0X5-TE63-A8B3"},{"doi-asserted-by":"publisher","key":"bibr9-s10024-003-8085-0","DOI":"10.1084\/jem.168.1.229"},{"doi-asserted-by":"publisher","key":"bibr10-s10024-003-8085-0","DOI":"10.1182\/blood.V87.12.5242.bloodjournal87125242"},{"doi-asserted-by":"publisher","key":"bibr11-s10024-003-8085-0","DOI":"10.1182\/blood.V81.3.775.775"},{"doi-asserted-by":"publisher","key":"bibr12-s10024-003-8085-0","DOI":"10.1172\/JCI115650"},{"doi-asserted-by":"publisher","key":"bibr13-s10024-003-8085-0","DOI":"10.3109\/10428199509056854"},{"doi-asserted-by":"publisher","key":"bibr14-s10024-003-8085-0","DOI":"10.1007\/BF02899394"},{"doi-asserted-by":"publisher","key":"bibr15-s10024-003-8085-0","DOI":"10.1002\/path.1711730410"},{"key":"bibr16-s10024-003-8085-0","first-page":"642","volume":"11","author":"Lai R","year":"1998","journal-title":"Mod Pathol"},{"key":"bibr17-s10024-003-8085-0","doi-asserted-by":"crossref","first-page":"3994","DOI":"10.4049\/jimmunol.141.11.3994","volume":"141","author":"Meeker TC","year":"1988","journal-title":"J Immunol"},{"doi-asserted-by":"publisher","key":"bibr18-s10024-003-8085-0","DOI":"10.1182\/blood.V80.9.2326.2326"},{"doi-asserted-by":"publisher","key":"bibr19-s10024-003-8085-0","DOI":"10.1038\/sj.leu.2400998"},{"key":"bibr20-s10024-003-8085-0","first-page":"925","volume":"79","author":"Nakamura N","year":"1999","journal-title":"Lab Invest"},{"doi-asserted-by":"publisher","key":"bibr21-s10024-003-8085-0","DOI":"10.1002\/j.1460-2075.1993.tb06189.x"},{"doi-asserted-by":"publisher","key":"bibr22-s10024-003-8085-0","DOI":"10.4049\/jimmunol.166.4.2235"},{"doi-asserted-by":"publisher","key":"bibr23-s10024-003-8085-0","DOI":"10.1146\/annurev.iy.12.040194.001001"},{"key":"bibr24-s10024-003-8085-0","doi-asserted-by":"crossref","first-page":"3347","DOI":"10.4049\/jimmunol.159.7.3347","volume":"159","author":"Razanajaona D","year":"1997","journal-title":"J Immunol"},{"doi-asserted-by":"publisher","key":"bibr25-s10024-003-8085-0","DOI":"10.1016\/S1074-7613(00)80240-X"},{"doi-asserted-by":"publisher","key":"bibr26-s10024-003-8085-0","DOI":"10.4049\/jimmunol.164.3.1306"},{"doi-asserted-by":"publisher","key":"bibr27-s10024-003-8085-0","DOI":"10.1126\/science.2448875"},{"key":"bibr28-s10024-003-8085-0","first-page":"442","volume":"14","author":"Koop BF","year":"1993","journal-title":"Biotechniques"},{"doi-asserted-by":"publisher","key":"bibr29-s10024-003-8085-0","DOI":"10.1111\/j.1600-0609.1999.tb01766.x"},{"doi-asserted-by":"publisher","key":"bibr30-s10024-003-8085-0","DOI":"10.1046\/j.1365-2141.1996.d01-1851.x"},{"doi-asserted-by":"publisher","key":"bibr31-s10024-003-8085-0","DOI":"10.1093\/intimm\/1.4.362"},{"doi-asserted-by":"publisher","key":"bibr32-s10024-003-8085-0","DOI":"10.1002\/eji.1830210532"},{"doi-asserted-by":"publisher","key":"bibr33-s10024-003-8085-0","DOI":"10.1002\/eji.1830201009"},{"doi-asserted-by":"publisher","key":"bibr34-s10024-003-8085-0","DOI":"10.1182\/blood.V75.7.1518.1518"},{"doi-asserted-by":"publisher","key":"bibr35-s10024-003-8085-0","DOI":"10.1111\/j.1365-3083.1989.tb02455.x"}],"container-title":["Pediatric and Developmental Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1007\/s10024-003-8085-0","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1007\/s10024-003-8085-0","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T10:17:35Z","timestamp":1741688255000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1007\/s10024-003-8085-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,5]]},"references-count":35,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2004,5]]}},"alternative-id":["10.1007\/s10024-003-8085-0"],"URL":"https:\/\/doi.org\/10.1007\/s10024-003-8085-0","relation":{},"ISSN":["1093-5266","1615-5742"],"issn-type":[{"type":"print","value":"1093-5266"},{"type":"electronic","value":"1615-5742"}],"subject":[],"published":{"date-parts":[[2004,5]]}}}