{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T13:05:33Z","timestamp":1787144733983,"version":"build-2736575974"},"reference-count":38,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2001,3,1]],"date-time":"2001-03-01T00:00:00Z","timestamp":983404800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatr Dev Pathol"],"published-print":{"date-parts":[[2001,3]]},"abstract":"<jats:p> Anaplastic large cell lymphoma (ALCL) was proposed as a clinicopathologic entity over 14 years ago, but has been somewhat controversial due to the variability of its defining features and variable occurrence in different age-groups. To evaluate this entity in a pediatric population, 36 cases of childhood large cell lymphoma were evaluated for abnormalities of the anaplastic lymphoma kinase (ALK) gene that has been associated with ALCL morphology and immunophenotype. ALK abnormalities were evaluated by assay for the t(2;5)(p23;q35) translocation by RT-PCR and\/or expression of NPM-ALK fusion protein by immunohistochemistry. Results showed 17 patients to have evidence of ALK gene expression. All of these children (mean age, 9.3 years) had tumors that were of T-cell phenotype (with the exception of a single case of null phenotype) and that expressed CD30. In contrast, 19 children with no evidence of ALK expression were older (mean, 12.7 years), and the majority (12\/19) had tumors of B-cell phenotype. CD30 was also diffusely expressed in 8 of these 19 tumors. The difference in mean age between the two groups was statistically significant ( P = 0.015). In three cases tested for both ALK and the t(2;5), ALK protein was detected in the absence of the t(2;5) translocation but no cases showed the reverse pattern, consistent with ALK fusion to genes other than NPM or activation of the ALK gene by another mechanism. These findings provide further support that ALK-positive ALCL is a distinct pathologic entity among pediatric large cell lymphomas primarily characterized by expression of T-cell markers, CD30, and EMA, and by a younger mean age. <\/jats:p>","DOI":"10.1007\/s100240010139","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T06:40:57Z","timestamp":1027579257000},"page":"129-137","source":"Crossref","is-referenced-by-count":14,"title":["Morphological and Phenotypic Features in Pediatric Large Cell Lymphoma and Their Correlation with ALK Expression and the t(2;5)(p23;q35) Translocation"],"prefix":"10.1177","volume":"4","author":[{"given":"C.G.","family":"Sherman","sequence":"first","affiliation":[{"name":"Department of Pediatric Laboratory Medicine, Division of Pathology, Hospital for Sick Children and University of Toronto, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Zielenska","sequence":"additional","affiliation":[{"name":"Department of Pediatric Laboratory Medicine, Division of Pathology, Hospital for Sick Children and University of Toronto, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.N.","family":"Lorenzana","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Hospital for Sick Children and University of Toronto, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.A.F.","family":"Pulford","sequence":"additional","affiliation":[{"name":"Leukaemia Research Fund Immunodiagnostics Unit, John Radcliffe Hospital, Oxford, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.Y.","family":"Mason","sequence":"additional","affiliation":[{"name":"Leukaemia Research Fund Immunodiagnostics Unit, John Radcliffe Hospital, Oxford, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.E.","family":"Hutchison","sequence":"additional","affiliation":[{"name":"Department of Pathology, State University of New York Health Science Center, Syracuse, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.S.","family":"Thorner","sequence":"additional","affiliation":[{"name":"Department of Pediatric Laboratory Medicine, Division of Pathology, Hospital for Sick Children and University of Toronto, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2001,3,1]]},"reference":[{"key":"bibr1-s100240010139","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(197410)34:8+<1488::AID-CNCR2820340822>3.0.CO;2-C"},{"key":"bibr2-s100240010139","first-page":"2112","volume":"49","author":"Working F.","year":"1982","journal-title":"Summary and description of a working formulation for clinical usage. 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