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This study aimed to clarify the impact of hypothermia on coagulation by eliminating clinical biases in vitro. In the hypothermic storage study, blood samples from five healthy volunteers were stored at 37\u00a0\u2103 (group N) for 3\u00a0h or at 20\u00a0\u2103 for 2\u00a0h, followed by 1\u00a0h of rewarming at 37\u00a0\u2103 (group H). Thromboelastography was performed before and after 3\u00a0h of storage. In the mock circulation loop (MCL) study, blood samples were placed in the MCL and (a) maintained at 37\u00a0\u2103 for 4\u00a0h (group N, <jats:italic>n<\/jats:italic>\u2009=\u20095), or (b) cooled to 20\u00a0\u2103 to simulate HCA with a 0.1\u00a0L\/min flow rate for 3\u00a0h and then rewarmed to 37\u00a0\u2103 (group H, <jats:italic>n<\/jats:italic>\u2009=\u20095). The total MCL duration was 4\u00a0h, and the flow rate was maintained at 1\u00a0L\/min, except during HCA. Blood samples collected 15\u00a0min after the beginning and end of MCL were subjected to standard laboratory tests and rotational thromboelastometry analyses. Hypothermia had no impact on coagulation in both the hypothermic storage and MCL studies. MCL significantly decreased the platelet counts and clot elasticity in the INTEM and EXTEM assays; however, there was no effect on fibrinogen contribution measured by FIBTEM. Hypothermia does not cause irreversible coagulopathy in vitro; however, MCL decreases coagulation due to the deterioration of platelets.<\/jats:p>","DOI":"10.1007\/s10047-022-01324-5","type":"journal-article","created":{"date-parts":[[2022,3,18]],"date-time":"2022-03-18T09:02:33Z","timestamp":1647594153000},"page":"314-322","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Hypothermic circulatory arrest does not induce coagulopathy in vitro"],"prefix":"10.1007","volume":"25","author":[{"given":"Hayato","family":"Ise","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8478-4417","authenticated-orcid":false,"given":"Kyohei","family":"Oyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shingo","family":"Kunioka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomonori","family":"Shirasaka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirotsugu","family":"Kanda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Payam","family":"Akhyari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Kamiya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,3,18]]},"reference":[{"key":"1324_CR1","doi-asserted-by":"publisher","first-page":"70","DOI":"10.14503\/THIJ-17-6364","volume":"45","author":"P Gupta","year":"2018","unstructured":"Gupta P, Harky A, Jahangeer S, et al. 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