{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T17:07:24Z","timestamp":1789578444667,"version":"build-2803163510"},"reference-count":31,"publisher":"Springer Science and Business Media LLC","issue":"10","license":[{"start":{"date-parts":[[2018,7,23]],"date-time":"2018-07-23T00:00:00Z","timestamp":1532304000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81771765"],"award-info":[{"award-number":["81771765"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81771766"],"award-info":[{"award-number":["81771766"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"clinical research fund of xiangya hospital, central south university","award":["2014l10"],"award-info":[{"award-number":["2014l10"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Clin Rheumatol"],"published-print":{"date-parts":[[2018,10]]},"DOI":"10.1007\/s10067-018-4216-x","type":"journal-article","created":{"date-parts":[[2018,7,23]],"date-time":"2018-07-23T02:07:24Z","timestamp":1532311644000},"page":"2731-2739","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Calcinosis and malignancy are rare in Chinese adult patients with myositis and nuclear matrix protein 2 antibodies identified by an unlabeled immunoprecipitation assay"],"prefix":"10.1007","volume":"37","author":[{"given":"Li","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanjuan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meidong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yizhi","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxia","family":"Zuo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yisha","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianzhong","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0788-7092","authenticated-orcid":false,"given":"Huali","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2018,7,23]]},"reference":[{"key":"4216_CR1","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1111\/joim.12451","volume":"280","author":"Z Betteridge","year":"2016","unstructured":"Betteridge Z, McHugh N (2016) Myositis-specific autoantibodies: an important tool to support diagnosis of myositis. J Intern Med 280:8\u201323. \n                    https:\/\/doi.org\/10.1111\/joim.12451","journal-title":"J Intern Med"},{"key":"4216_CR2","doi-asserted-by":"publisher","first-page":"1044","DOI":"10.1016\/j.autrev.2017.07.021","volume":"16","author":"S Gao","year":"2017","unstructured":"Gao S, Luo H, Zhang H, Zuo X, Wang L, Zhu H (2017) Using multi-omics methods to understand dermatomyositis\/polymyositis. Autoimmun Rev 16:1044\u20131048. \n                    https:\/\/doi.org\/10.1016\/j.autrev.2017.07.021","journal-title":"Autoimmun Rev"},{"key":"4216_CR3","doi-asserted-by":"publisher","first-page":"602","DOI":"10.1097\/BOR.0b013e328358bd85","volume":"24","author":"L Casciola-Rosen","year":"2012","unstructured":"Casciola-Rosen L, Mammen AL (2012) Myositis autoantibodies. Curr Opin Rheumatol 24:602\u2013608. \n                    https:\/\/doi.org\/10.1097\/BOR.0b013e328358bd85","journal-title":"Curr Opin Rheumatol"},{"key":"4216_CR4","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1080\/08916930400022640","volume":"38","author":"S Zampieri","year":"2005","unstructured":"Zampieri S, Ghirardello A, Iaccarino L, Tarricone E, Gambari PF, Doria A (2005) Anti-Jo-1 antibodies. Autoimmunity 38:73\u201378. \n                    https:\/\/doi.org\/10.1080\/08916930400022640","journal-title":"Autoimmunity"},{"key":"4216_CR5","first-page":"1012","volume":"34","author":"T Matsushita","year":"2007","unstructured":"Matsushita T, Hasegawa M, Fujimoto M, Hamaguchi Y, Komura K, Hirano T, Horikawa M, Kondo M, Orito H, Kaji K, Saito Y, Matsushita Y, Kawara S, Yasui M, Seishima M, Ozaki S, Kuwana M, Ogawa F, Sato S, Takehara K (2007) Clinical evaluation of anti-aminoacyl tRNA synthetase antibodies in Japanese patients with dermatomyositis. J Rheumatol 34:1012\u20131018","journal-title":"J Rheumatol"},{"key":"4216_CR6","doi-asserted-by":"publisher","first-page":"1635","DOI":"10.1136\/ard.2006.052191","volume":"65","author":"GJ Hengstman","year":"2006","unstructured":"Hengstman GJ, ter Laak HJ, Vree Egberts WT, Lundberg IE, Moutsopoulos HM, Vencovsky J, Doria A, Mosca M, van Venrooij WJ, van Engelen BG (2006) Anti-signal recognition particle autoantibodies: marker of a necrotising myopathy. Ann Rheum Dis 65:1635\u20131638. \n                    https:\/\/doi.org\/10.1136\/ard.2006.052191","journal-title":"Ann Rheum Dis"},{"key":"4216_CR7","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1016\/j.jdermsci.2005.09.004","volume":"40","author":"K Komura","year":"2005","unstructured":"Komura K, Fujimoto M, Matsushita T, Kaji K, Kondo M, Hirano T, Orito H, Horikawa M, Hamaguchi Y, Hasegawa M, Takehara K, Sato S (2005) Prevalence and clinical characteristics of anti-Mi-2 antibodies in Japanese patients with dermatomyositis. J Dermatol Sci 40:215\u2013217. \n                    https:\/\/doi.org\/10.1016\/j.jdermsci.2005.09.004","journal-title":"J Dermatol Sci"},{"key":"4216_CR8","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1080\/08916930400022681","volume":"38","author":"A Ghirardello","year":"2005","unstructured":"Ghirardello A, Zampieri S, Iaccarino L, Tarricone E, Bendo R, Gambari PF, Doria A (2005) Anti-Mi-2 antibodies. Autoimmunity 38:79\u201383","journal-title":"Autoimmunity"},{"key":"4216_CR9","doi-asserted-by":"publisher","first-page":"242","DOI":"10.1136\/ard.2005.040717","volume":"65","author":"GJ Hengstman","year":"2006","unstructured":"Hengstman GJ, Vree Egberts WT, Seelig HP, Lundberg IE, Moutsopoulos HM, Doria A, Mosca M, Vencovsky J, van Venrooij WJ, van Engelen BG (2006) Clinical characteristics of patients with myositis and autoantibodies to different fragments of the Mi-2 beta antigen. Ann Rheum Dis 65:242\u2013245. \n                    https:\/\/doi.org\/10.1136\/ard.2005.040717","journal-title":"Ann Rheum Dis"},{"key":"4216_CR10","doi-asserted-by":"publisher","first-page":"1571","DOI":"10.1002\/art.21023","volume":"52","author":"S Sato","year":"2005","unstructured":"Sato S, Hirakata M, Kuwana M, Suwa A, Inada S, Mimori T, Nishikawa T, Oddis CV, Ikeda Y (2005) Autoantibodies to a 140-kd polypeptide, CADM-140, in Japanese patients with clinically amyopathic dermatomyositis. Arthritis Rheum 52:1571\u20131576. \n                    https:\/\/doi.org\/10.1002\/art.21023","journal-title":"Arthritis Rheum"},{"key":"4216_CR11","doi-asserted-by":"publisher","first-page":"3682","DOI":"10.1002\/art.22164","volume":"54","author":"IN Targoff","year":"2006","unstructured":"Targoff IN, Mamyrova G, Trieu EP, Perurena O, Koneru B, O'Hanlon TP, Miller FW, Rider LG, Childhood Myositis Heterogeneity Study G, International Myositis Collaborative Study G (2006) A novel autoantibody to a 155-kd protein is associated with dermatomyositis. Arthritis Rheum 54:3682\u20133689. \n                    https:\/\/doi.org\/10.1002\/art.22164","journal-title":"Arthritis Rheum"},{"key":"4216_CR12","doi-asserted-by":"publisher","first-page":"1807","DOI":"10.1002\/art.24547","volume":"60","author":"H Gunawardena","year":"2009","unstructured":"Gunawardena H, Wedderburn LR, Chinoy H, Betteridge ZE, North J, Ollier WE, Cooper RG, Oddis CV, Ramanan AV, Davidson JE, McHugh NJ, Juvenile Dermatomyositis Research Group UK, Ireland (2009) Autoantibodies to a 140-kd protein in juvenile dermatomyositis are associated with calcinosis. Arthritis Rheum 60:1807\u20131814. \n                    https:\/\/doi.org\/10.1002\/art.24547","journal-title":"Arthritis Rheum"},{"key":"4216_CR13","doi-asserted-by":"publisher","first-page":"2547","DOI":"10.3899\/jrheum.090461","volume":"36","author":"G Espada","year":"2009","unstructured":"Espada G, Maldonado Cocco JA, Fertig N, Oddis CV (2009) Clinical and serologic characterization of an Argentine pediatric myositis cohort: identification of a novel autoantibody (anti-MJ) to a 142-kDa protein. J Rheumatol 36:2547\u20132551. \n                    https:\/\/doi.org\/10.3899\/jrheum.090461","journal-title":"J Rheumatol"},{"key":"4216_CR14","doi-asserted-by":"publisher","first-page":"1701","DOI":"10.1091\/mbc.e06-08-0747","volume":"18","author":"K Takahashi","year":"2007","unstructured":"Takahashi K, Yoshida N, Murakami N, Kawata K, Ishizaki H, Tanaka-Okamoto M, Miyoshi J, Zinn AR, Shime H, Inoue N (2007) Dynamic regulation of p53 subnuclear localization and senescence by MORC3. Mol Biol Cell 18:1701\u20131709. \n                    https:\/\/doi.org\/10.1091\/mbc.E06-08-0747","journal-title":"Mol Biol Cell"},{"key":"4216_CR15","doi-asserted-by":"publisher","first-page":"2014","DOI":"10.1242\/jcs.063586","volume":"123","author":"Y Mimura","year":"2010","unstructured":"Mimura Y, Takahashi K, Kawata K, Akazawa T, Inoue N (2010) Two-step colocalization of MORC3 with PML nuclear bodies. J Cell Sci 123:2014\u20132024. \n                    https:\/\/doi.org\/10.1242\/jcs.063586","journal-title":"J Cell Sci"},{"key":"4216_CR16","doi-asserted-by":"publisher","first-page":"R97","DOI":"10.1186\/ar3822","volume":"14","author":"A Ceribelli","year":"2012","unstructured":"Ceribelli A, Fredi M, Taraborelli M, Cavazzana I, Franceschini F, Quinzanini M, Tincani A, Ross SJ, Chan JY, Pauley BA, Chan EK, Satoh M (2012) Anti-MJ\/NXP-2 autoantibody specificity in a cohort of adult Italian patients with polymyositis\/dermatomyositis. Arthritis Res Ther 14:R97. \n                    https:\/\/doi.org\/10.1186\/ar3822","journal-title":"Arthritis Res Ther"},{"key":"4216_CR17","doi-asserted-by":"publisher","first-page":"710","DOI":"10.1136\/annrheumdis-2011-200697","volume":"71","author":"Y Ichimura","year":"2012","unstructured":"Ichimura Y, Matsushita T, Hamaguchi Y, Kaji K, Hasegawa M, Tanino Y, Inokoshi Y, Kawai K, Kanekura T, Habuchi M, Igarashi A, Sogame R, Hashimoto T, Koga T, Nishino A, Ishiguro N, Sugimoto N, Aoki R, Ando N, Abe T, Kanda T, Kuwana M, Takehara K, Fujimoto M (2012) Anti-NXP2 autoantibodies in adult patients with idiopathic inflammatory myopathies: possible association with malignancy. Ann Rheum Dis 71:710\u2013713. \n                    https:\/\/doi.org\/10.1136\/annrheumdis-2011-200697","journal-title":"Ann Rheum Dis"},{"key":"4216_CR18","doi-asserted-by":"publisher","first-page":"1211","DOI":"10.1016\/j.autrev.2014.08.011","volume":"13","author":"L Bodoki","year":"2014","unstructured":"Bodoki L, Nagy-Vincze M, Griger Z, Betteridge Z, Sz\u00f6ll\u00f6si L, Dank\u00f3 K (2014) Four dermatomyositis-specific autoantibodies-anti-TIF1gamma, anti-NXP2, anti-SAE and anti-MDA5-in adult and juvenile patients with idiopathic inflammatory myopathies in a Hungarian cohort. Autoimmun Rev 13:1211\u20131219. \n                    https:\/\/doi.org\/10.1016\/j.autrev.2014.08.011","journal-title":"Autoimmun Rev"},{"key":"4216_CR19","doi-asserted-by":"publisher","first-page":"724","DOI":"10.1001\/jamadermatol.2013.10416","volume":"150","author":"A Valenzuela","year":"2014","unstructured":"Valenzuela A, Chung L, Casciola-Rosen L, Fiorentino D (2014) Identification of clinical features and autoantibodies associated with calcinosis in dermatomyositis. JAMA Dermatol 150:724\u2013729. \n                    https:\/\/doi.org\/10.1001\/jamadermatol.2013.10416","journal-title":"JAMA Dermatol"},{"key":"4216_CR20","doi-asserted-by":"publisher","first-page":"1771","DOI":"10.1002\/acr.23188","volume":"69","author":"J Albayda","year":"2017","unstructured":"Albayda J, Pinal-Fernandez I, Huang W, Parks C, Paik J, Casciola-Rosen L, Danoff SK, Johnson C, Christopher-Stine L, Mammen AL (2017) Dermatomyositis patients with anti-nuclear matrix protein-2 autoantibodies have more edema, more severe muscle disease, and increased malignancy risk. Arthritis Care Res 69:1771\u20131776. \n                    https:\/\/doi.org\/10.1002\/acr.23188","journal-title":"Arthritis Care Res"},{"key":"4216_CR21","doi-asserted-by":"publisher","first-page":"469","DOI":"10.1007\/s10067-016-3453-0","volume":"36","author":"A Ceribelli","year":"2017","unstructured":"Ceribelli A, Isailovic N, De Santis M, Generali E, Fredi M, Cavazzana I, Franceschini F, Cantarini L, Satoh M, Selmi C (2017) Myositis-specific autoantibodies and their association with malignancy in Italian patients with polymyositis and dermatomyositis. Clin Rheumatol 36:469\u2013475. \n                    https:\/\/doi.org\/10.1007\/s10067-016-3453-0","journal-title":"Clin Rheumatol"},{"key":"4216_CR22","doi-asserted-by":"publisher","first-page":"1909","DOI":"10.1002\/acr.23210","volume":"69","author":"A Rogers","year":"2017","unstructured":"Rogers A, Chung L, Li S, Casciola-Rosen L, Fiorentino DF (2017) The cutaneous and systemic findings associated with nuclear matrix protein-2 antibodies in adult dermatomyositis patients. Arthritis Care Res (Hoboken) 69:1909\u20131914. \n                    https:\/\/doi.org\/10.1002\/acr.23210","journal-title":"Arthritis Care Res (Hoboken)"},{"key":"4216_CR23","doi-asserted-by":"publisher","first-page":"344","DOI":"10.1056\/NEJM197502132920706","volume":"292","author":"A Bohan","year":"1975","unstructured":"Bohan A, Peter JB (1975) Polymyositis and dermatomyositis (first of two parts). N Engl J Med 292:344\u2013347. \n                    https:\/\/doi.org\/10.1056\/NEJM197502132920706","journal-title":"N Engl J Med"},{"key":"4216_CR24","doi-asserted-by":"publisher","first-page":"1345","DOI":"10.1136\/ard.2006.068502","volume":"66","author":"H Chinoy","year":"2007","unstructured":"Chinoy H, Fertig N, Oddis CV, Ollier WE, Cooper RG (2007) The diagnostic utility of myositis autoantibody testing for predicting the risk of cancer-associated myositis. Ann Rheum Dis 66:1345\u20131349. \n                    https:\/\/doi.org\/10.1136\/ard.2006.068502","journal-title":"Ann Rheum Dis"},{"key":"4216_CR25","doi-asserted-by":"publisher","first-page":"2556","DOI":"10.1172\/JCI116493","volume":"91","author":"IN Targoff","year":"1993","unstructured":"Targoff IN, Trieu EP, Miller FW (1993) Reaction of anti-OJ autoantibodies with components of the multi-enzyme complex of aminoacyl-tRNA synthetases in addition to isoleucyl-tRNA synthetase. J Clin Invest 91:2556\u20132564. \n                    https:\/\/doi.org\/10.1172\/JCI116493","journal-title":"J Clin Invest"},{"key":"4216_CR26","doi-asserted-by":"publisher","first-page":"2204","DOI":"10.1093\/rheumatology\/keu259","volume":"53","author":"SL Tansley","year":"2014","unstructured":"Tansley SL, Betteridge ZE, Shaddick G, Gunawardena H, Arnold K, Wedderburn LR, McHugh NJ, Juvenile Dermatomyositis Research Group (2014) Calcinosis in juvenile dermatomyositis is influenced by both anti-NXP2 autoantibody status and age at disease onset. Rheumatology (Oxford) 53:2204\u20132208. \n                    https:\/\/doi.org\/10.1093\/rheumatology\/keu259","journal-title":"Rheumatology (Oxford)"},{"key":"4216_CR27","doi-asserted-by":"publisher","first-page":"2954","DOI":"10.1002\/art.38093","volume":"65","author":"DF Fiorentino","year":"2013","unstructured":"Fiorentino DF, Chung LS, Christopher-Stine L, Zaba L, Li S, Mammen AL, Rosen A, Casciola-Rosen L (2013) Most patients with cancer-associated dermatomyositis have antibodies to nuclear matrix protein NXP-2 or transcription intermediary factor 1gamma. Arthritis Rheum 65:2954\u20132962. \n                    https:\/\/doi.org\/10.1002\/art.38093","journal-title":"Arthritis Rheum"},{"key":"4216_CR28","doi-asserted-by":"publisher","first-page":"1181","DOI":"10.1093\/rheumatology\/kes033","volume":"51","author":"A Ishikawa","year":"2012","unstructured":"Ishikawa A, Muro Y, Sugiura K, Akiyama M (2012) Development of an ELISA for detection of autoantibodies to nuclear matrix protein 2. Rheumatology (Oxford) 51:1181\u20131187. \n                    https:\/\/doi.org\/10.1093\/rheumatology\/kes033","journal-title":"Rheumatology (Oxford)"},{"key":"4216_CR29","doi-asserted-by":"publisher","first-page":"713","DOI":"10.1002\/art.30156","volume":"63","author":"AL Mammen","year":"2011","unstructured":"Mammen AL, Chung T, Christopher-Stine L, Rosen P, Rosen A, Doering KR, Casciola-Rosen LA (2011) Autoantibodies against 3-hydroxy-3-methylglutaryl-coenzyme a reductase in patients with statin-associated autoimmune myopathy. Arthritis Rheum 63:713\u2013721. \n                    https:\/\/doi.org\/10.1002\/art.30156","journal-title":"Arthritis Rheum"},{"key":"4216_CR30","doi-asserted-by":"publisher","first-page":"799","DOI":"10.2169\/internalmedicine.51.6566","volume":"51","author":"K Sugie","year":"2012","unstructured":"Sugie K, Tonomura Y, Ueno S (2012) Characterization of dermatomyositis with coexistence of anti-Jo-1 and anti-SRP antibodies. Intern Med 51:799\u2013802","journal-title":"Intern Med"},{"key":"4216_CR31","doi-asserted-by":"publisher","first-page":"1038","DOI":"10.1136\/jnnp-2016-313166","volume":"87","author":"Y Watanabe","year":"2016","unstructured":"Watanabe Y, Uruha A, Suzuki S, Nakahara J, Hamanaka K, Takayama K, Suzuki N, Nishino I (2016) Clinical features and prognosis in anti-SRP and anti-HMGCR necrotising myopathy. J Neurol Neurosurg Psychiatry 87:1038\u20131044. \n                    https:\/\/doi.org\/10.1136\/jnnp-2016-313166","journal-title":"J Neurol Neurosurg Psychiatry"}],"container-title":["Clinical Rheumatology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s10067-018-4216-x\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10067-018-4216-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10067-018-4216-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,23]],"date-time":"2019-09-23T09:40:12Z","timestamp":1569231612000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s10067-018-4216-x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,7,23]]},"references-count":31,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2018,10]]}},"alternative-id":["4216"],"URL":"https:\/\/doi.org\/10.1007\/s10067-018-4216-x","relation":{},"ISSN":["0770-3198","1434-9949"],"issn-type":[{"value":"0770-3198","type":"print"},{"value":"1434-9949","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,7,23]]},"assertion":[{"value":"21 March 2018","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 July 2018","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 July 2018","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 July 2018","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"The study is in accordance with the ethical principles of the Declaration of Helsinki. The study protocol was approved by the Institutional Review Board at the Xiangya Hospital of Central South University (approval number: 201703567). All study participants provided written informed consent prior to participation in the study.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval and consent to participate"}},{"value":"None.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Disclosures"}}]}}