{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T00:51:55Z","timestamp":1791593515637,"version":"4.3.4"},"reference-count":25,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2014,10,8]],"date-time":"2014-10-08T00:00:00Z","timestamp":1412726400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Neurol Sci"],"published-print":{"date-parts":[[2015,3]]},"DOI":"10.1007\/s10072-014-1971-6","type":"journal-article","created":{"date-parts":[[2014,10,7]],"date-time":"2014-10-07T00:54:39Z","timestamp":1412643279000},"page":"429-434","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Selection and characterization of llama single domain antibodies against N-terminal huntingtin"],"prefix":"10.1007","volume":"36","author":[{"given":"Menno H.","family":"Schut","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barry A.","family":"Pepers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rinse","family":"Klooster","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silv\u00e8re M.","family":"van der Maarel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed","family":"el Khatabi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theo","family":"Verrips","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johan T.","family":"den Dunnen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gert-Jan B.","family":"van Ommen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Willeke M. C.","family":"van Roon-Mom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2014,10,8]]},"reference":[{"key":"1971_CR1","doi-asserted-by":"crossref","unstructured":"(1993) A novel gene containing a trinucleotide repeat that is expanded and unstable on Huntington\u2019s disease chromosomes. The Huntington\u2019s Disease Collaborative Research Group.\u00a0Cell 72(6): 971\u2013983","DOI":"10.1016\/0092-8674(93)90585-E"},{"issue":"14","key":"1971_CR2","doi-asserted-by":"crossref","first-page":"2846","DOI":"10.1093\/hmg\/ddr195","volume":"20","author":"JC Jacobsen","year":"2011","unstructured":"Jacobsen JC, Gregory GC, Woda JM et al (2011) HD CAG-correlated gene expression changes support a simple dominant gain of function. Hum Mol Genet 20(14):2846\u20132860. doi: 10.1093\/hmg\/ddr195","journal-title":"Hum Mol Genet"},{"issue":"32","key":"1971_CR3","doi-asserted-by":"crossref","first-page":"11563","DOI":"10.1073\/pnas.0505321102","volume":"102","author":"WJ Wolfgang","year":"2005","unstructured":"Wolfgang WJ, Miller TW, Webster JM et al (2005) Suppression of Huntington\u2019s disease pathology in Drosophila by human single-chain Fv antibodies. Proc Natl Acad Sci USA 102(32):11563\u201311568. doi: 10.1073\/pnas.0505321102","journal-title":"Proc Natl Acad Sci USA"},{"issue":"10","key":"1971_CR4","doi-asserted-by":"crossref","first-page":"1078","DOI":"10.1097\/NEN.0b013e3181f530ec","volume":"69","author":"A Snyder-Keller","year":"2010","unstructured":"Snyder-Keller A, McLear JA, Hathorn T, Messer A (2010) Early or late-stage anti-N-terminal Huntingtin intrabody gene therapy reduces pathological features in B6.HDR6\/1 mice. J Neuropathol Exp Neurol 69(10):1078\u20131085. doi: 10.1097\/NEN.0b013e3181f530ec","journal-title":"J Neuropathol Exp Neurol"},{"issue":"6428","key":"1971_CR5","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1038\/363446a0","volume":"363","author":"C Hamers-Casterman","year":"1993","unstructured":"Hamers-Casterman C, Atarhouch T, Muyldermans S et al (1993) Naturally occurring antibodies devoid of light chains. Nature 363(6428):446\u2013448. doi: 10.1038\/363446a0","journal-title":"Nature"},{"issue":"1","key":"1971_CR6","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/S0167-4838(99)00030-8","volume":"1431","author":"RH Linden van der","year":"1999","unstructured":"van der Linden RH, Frenken LG, de Geus B et al (1999) Comparison of physical chemical properties of llama VHH antibody fragments and mouse monoclonal antibodies. Biochim Biophys Acta 1431(1):37\u201346","journal-title":"Biochim Biophys Acta"},{"issue":"3","key":"1971_CR7","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1016\/S0014-5793(97)01062-4","volume":"414","author":"M Arbabi Ghahroudi","year":"1997","unstructured":"Arbabi Ghahroudi M, Desmyter A, Wyns L et al (1997) Selection and identification of single domain antibody fragments from camel heavy-chain antibodies. FEBS Lett 414(3):521\u2013526","journal-title":"FEBS Lett"},{"issue":"1","key":"1971_CR8","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1093\/hmg\/ddi432","volume":"15","author":"P Verheesen","year":"2006","unstructured":"Verheesen P, de Kluijver A, van Koningsbruggen S et al (2006) Prevention of oculopharyngeal muscular dystrophy-associated aggregation of nuclear polyA-binding protein with a single-domain intracellular antibody. Hum Mol Genet 15(1):105\u2013111. doi: 10.1093\/hmg\/ddi432","journal-title":"Hum Mol Genet"},{"issue":"10","key":"1971_CR9","doi-asserted-by":"crossref","first-page":"1849","DOI":"10.1093\/hmg\/ddp101","volume":"18","author":"A Chartier","year":"2009","unstructured":"Chartier A, Raz V, Sterrenburg E et al (2009) Prevention of oculopharyngeal muscular dystrophy by muscular expression of Llama single-chain intrabodies in vivo. Hum Mol Genet 18(10):1849\u20131859. doi: 10.1093\/hmg\/ddp101","journal-title":"Hum Mol Genet"},{"key":"1971_CR10","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1186\/1472-6750-9-50","volume":"9","author":"BA Pepers","year":"2009","unstructured":"Pepers BA, Schut MH, Vossen RH et al (2009) Cost-effective HRMA pre-sequence typing of clone libraries; application to phage display selection. BMC Biotechnol 9:50. doi: 10.1186\/1472-6750-9-50","journal-title":"BMC Biotechnol"},{"issue":"5","key":"1971_CR11","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1006\/jmbi.2001.5276","volume":"315","author":"B Liu","year":"2002","unstructured":"Liu B, Huang L, Sihlbom C et al (2002) Towards proteome-wide production of monoclonal antibody by phage display. J Mol Biol 315(5):1063\u20131073. doi: 10.1006\/jmbi.2001.5276","journal-title":"J Mol Biol"},{"issue":"2","key":"1971_CR12","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/S1097-2765(02)00602-0","volume":"10","author":"A Lunkes","year":"2002","unstructured":"Lunkes A, Lindenberg KS, Ben-Haiem L et al (2002) Proteases acting on mutant huntingtin generate cleaved products that differentially build up cytoplasmic and nuclear inclusions. Mol Cell 10(2):259\u2013269","journal-title":"Mol Cell"},{"issue":"5","key":"1971_CR13","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1038\/nri2763","volume":"10","author":"O Leavy","year":"2010","unstructured":"Leavy O (2010) Therapeutic antibodies: past, present and future. Nat Rev Immunol 10(5):297. doi: 10.1038\/nri2763","journal-title":"Nat Rev Immunol"},{"key":"1971_CR14","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.neuroscience.2011.05.076","volume":"190","author":"KS Rutgers","year":"2011","unstructured":"Rutgers KS, Nabuurs RJ, van den Berg SA et al (2011) Transmigration of beta amyloid specific heavy chain antibody fragments across the in vitro blood-brain barrier. Neuroscience 190:37\u201342. doi: 10.1016\/j.neuroscience.2011.05.076","journal-title":"Neuroscience"},{"issue":"10","key":"1971_CR15","doi-asserted-by":"crossref","first-page":"3969","DOI":"10.1096\/fj.11-201384","volume":"26","author":"T Li","year":"2012","unstructured":"Li T, Bourgeois JP, Celli S et al (2012) Cell-penetrating anti-GFAP VHH and corresponding fluorescent fusion protein VHH-GFP spontaneously cross the blood-brain barrier and specifically recognize astrocytes: application to brain imaging. FASEB J 26(10):3969\u20133979. doi: 10.1096\/fj.11-201384","journal-title":"FASEB J"},{"issue":"8","key":"1971_CR16","doi-asserted-by":"crossref","first-page":"1307","DOI":"10.1016\/j.bbapap.2006.05.011","volume":"1764","author":"P Verheesen","year":"2006","unstructured":"Verheesen P, Roussis A, de Haard HJ et al (2006) Reliable and controllable antibody fragment selections from Camelid non-immune libraries for target validation. Biochim Biophys Acta 1764(8):1307\u20131319. doi: 10.1016\/j.bbapap.2006.05.011","journal-title":"Biochim Biophys Acta"},{"issue":"1","key":"1971_CR17","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1523\/JNEUROSCI.1409-03.2004","volume":"24","author":"ZH Qin","year":"2004","unstructured":"Qin ZH, Wang Y, Sapp E et al (2004) Huntingtin bodies sequester vesicle-associated proteins by a polyproline-dependent interaction. J Neurosci 24(1):269\u2013281. doi: 10.1523\/JNEUROSCI.1409-03.2004","journal-title":"J Neurosci"},{"issue":"3","key":"1971_CR18","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1038\/81593","volume":"26","author":"I Dragatsis","year":"2000","unstructured":"Dragatsis I, Levine MS, Zeitlin S (2000) Inactivation of Hdh in the brain and testis results in progressive neurodegeneration and sterility in mice. Nat Genet 26(3):300\u2013306. doi: 10.1038\/81593","journal-title":"Nat Genet"},{"issue":"12","key":"1971_CR19","doi-asserted-by":"crossref","first-page":"8808","DOI":"10.1074\/jbc.M109.075028","volume":"285","author":"C Landles","year":"2010","unstructured":"Landles C, Sathasivam K, Weiss A et al (2010) Proteolysis of mutant huntingtin produces an exon 1 fragment that accumulates as an aggregated protein in neuronal nuclei in Huntington disease. J Biol Chem 285(12):8808\u20138823. doi: 10.1074\/jbc.M109.075028","journal-title":"J Biol Chem"},{"issue":"6","key":"1971_CR20","doi-asserted-by":"crossref","first-page":"e38284","DOI":"10.1371\/journal.pone.0038284","volume":"7","author":"RJ Nabuurs","year":"2012","unstructured":"Nabuurs RJ, Rutgers KS, Welling MM et al (2012) In vivo detection of amyloid-beta deposits using heavy chain antibody fragments in a transgenic mouse model for Alzheimer\u2019s disease. PLoS ONE 7(6):e38284. doi: 10.1371\/journal.pone.0038284","journal-title":"PLoS ONE"},{"issue":"2","key":"1971_CR21","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1038\/ng0298-150","volume":"18","author":"D Martindale","year":"1998","unstructured":"Martindale D, Hackam A, Wieczorek A et al (1998) Length of huntingtin and its polyglutamine tract influences localization and frequency of intracellular aggregates. Nat Genet 18(2):150\u2013154. doi: 10.1038\/ng0298-150","journal-title":"Nat Genet"},{"issue":"3","key":"1971_CR22","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1016\/0022-2836(91)90498-U","volume":"222","author":"JD Marks","year":"1991","unstructured":"Marks JD, Hoogenboom HR, Bonnert TP et al (1991) By-passing immunization. Human antibodies from V-gene libraries displayed on phage. J Mol Biol 222(3):581\u2013597","journal-title":"J Mol Biol"},{"issue":"1\u20133","key":"1971_CR23","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.bbagen.2003.09.006","volume":"1624","author":"P Verheesen","year":"2003","unstructured":"Verheesen P, ten Haaft MR, Lindner N et al (2003) Beneficial properties of single-domain antibody fragments for application in immunoaffinity purification and immuno-perfusion chromatography. Biochim Biophys Acta 1624(1\u20133):21\u201328","journal-title":"Biochim Biophys Acta"},{"issue":"5","key":"1971_CR24","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1038\/nbt.1539","volume":"27","author":"J Hu","year":"2009","unstructured":"Hu J, Matsui M, Gagnon KT et al (2009) Allele-specific silencing of mutant huntingtin and ataxin-3 genes by targeting expanded CAG repeats in mRNAs. Nat Biotechnol 27(5):478\u2013484. doi: 10.1038\/nbt.1539","journal-title":"Nat Biotechnol"},{"key":"1971_CR25","unstructured":"Kabat E, Wu TT, Perry H et al. (1991) United States Public Health Services Publication No. 91-3242 (National Institutes of Health, Bethesda, MD)"}],"container-title":["Neurological Sciences"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10072-014-1971-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s10072-014-1971-6\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10072-014-1971-6","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,3,26]],"date-time":"2019-03-26T18:52:19Z","timestamp":1553626339000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s10072-014-1971-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,10,8]]},"references-count":25,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2015,3]]}},"alternative-id":["1971"],"URL":"https:\/\/doi.org\/10.1007\/s10072-014-1971-6","relation":{},"ISSN":["1590-1874","1590-3478"],"issn-type":[{"value":"1590-1874","type":"print"},{"value":"1590-3478","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,10,8]]}}}