{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T21:41:03Z","timestamp":1790286063683,"version":"4.1.0"},"reference-count":43,"publisher":"Springer Science and Business Media LLC","issue":"8","license":[{"start":{"date-parts":[[2018,5,8]],"date-time":"2018-05-08T00:00:00Z","timestamp":1525737600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/501100004484","name":"Tehran University of Medical Sciences and Health Services","doi-asserted-by":"publisher","award":["29999-160-03-94"],"award-info":[{"award-number":["29999-160-03-94"]}],"id":[{"id":"10.13039\/501100004484","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Eur J Clin Microbiol Infect Dis"],"published-print":{"date-parts":[[2018,8]]},"DOI":"10.1007\/s10096-018-3266-y","type":"journal-article","created":{"date-parts":[[2018,5,8]],"date-time":"2018-05-08T06:34:41Z","timestamp":1525761281000},"page":"1421-1429","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Detection of toxoplasma-specific immunoglobulin G in human sera: performance comparison of in house Dot-ELISA with ECLIA and ELISA"],"prefix":"10.1007","volume":"37","author":[{"given":"Aref","family":"Teimouri","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Hossein","family":"Modarressi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saeedeh","family":"Shojaee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehdi","family":"Mohebali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nima","family":"Zouei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mostafa","family":"Rezaian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hossein","family":"Keshavarz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2018,5,8]]},"reference":[{"key":"3266_CR1","unstructured":"Dubey JP (2010). Toxoplasmosis of animals and humans. 2rd edn. CRC Press Inc. Boca Raton, New York, pp 1\u2013313"},{"key":"3266_CR2","first-page":"4","volume":"16","author":"C Alvarado-Esquirel","year":"2011","unstructured":"Alvarado-Esquirel C, Estrada-Martinez S, Liesenfeld O (2011) Toxoplasma gondii infection in workers occupationally exposed to unwashed raw fruits and vegetables: a case control seroprevalence study. Parasit Vectors 16:4\u2013235","journal-title":"Parasit Vectors"},{"key":"3266_CR3","doi-asserted-by":"publisher","first-page":"1217","DOI":"10.1016\/S0020-7519(00)00124-7","volume":"30","author":"AM Tenter","year":"2000","unstructured":"Tenter AM, Heckeroth AR, Weiss LM (2000) Toxoplasma gondii: from animals to humans. Int J Parasitol 30:1217\u20131258","journal-title":"Int J Parasitol"},{"key":"3266_CR4","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1186\/1756-3305-6-15","volume":"6","author":"F Walle","year":"2013","unstructured":"Walle F, Kebede N, Tsegaye A, Kassa T (2013) Seroprevalence and risk factors for toxoplasmosis in HIV infected and non-infected individuals in Bahir Dar, Northwest Ethiopia. Parasit Vectors 6:15\u201310","journal-title":"Parasit Vectors"},{"issue":"suppl A","key":"3266_CR5","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1093\/jac\/23.suppl_A.77","volume":"23","author":"CU Tuazon","year":"1989","unstructured":"Tuazon CU (1989) Toxoplasmosis in AIDS patients. J Antimicrob Chemother 23(suppl A):77\u201382","journal-title":"J Antimicrob Chemother"},{"key":"3266_CR6","doi-asserted-by":"publisher","first-page":"264","DOI":"10.1128\/CMR.05013-11","volume":"25","author":"F Robert-Gangneux","year":"2012","unstructured":"Robert-Gangneux F, Darde ML (2012) Epidemiology of and diagnostic strategies for toxoplasmosis. Clin Microbiol Rev 25:264\u2013296","journal-title":"Clin Microbiol Rev"},{"issue":"1","key":"3266_CR7","doi-asserted-by":"publisher","first-page":"292","DOI":"10.1186\/s13071-015-0902-6","volume":"8","author":"Q Liu","year":"2015","unstructured":"Liu Q, Wang Z-D, Huang S-Y, Zhu X-Q (2015) Diagnosis of toxoplasmosis and typing of Toxoplasma gondii. Parasit Vectors 8(1):292","journal-title":"Parasit Vectors"},{"issue":"6","key":"3266_CR8","doi-asserted-by":"publisher","first-page":"32227","DOI":"10.1038\/srep32227","volume":"2016","author":"D Acharya","year":"2016","unstructured":"Acharya D, Bastola P, Le L, Paul AM, Fernandez E, Diamond MS et al (2016) An ultrasensitive electrogenerated chemiluminescence-based immunoassay for specific detection of Zika virus. Sci Rep 2016(6):32227","journal-title":"Sci Rep"},{"key":"3266_CR9","doi-asserted-by":"publisher","first-page":"492","DOI":"10.1016\/j.pt.2016.04.001","volume":"32","author":"C Dard","year":"2016","unstructured":"Dard C, Fricker-Hidalgo H, Brenier-Pinchart MP, Pelloux H (2016) Relevance of and new developments in serology for toxoplasmosis. Trends Parasitol 32:492\u2013506","journal-title":"Trends Parasitol"},{"key":"3266_CR10","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1016\/0022-1759(83)90399-X","volume":"64","author":"MG Pappas","year":"1983","unstructured":"Pappas MG, Hajkowski R, Hockmeyer WT (1983) Dot enzyme-linked immunosorbent assay (Dot-ELISA): a micro technique for the rapid diagnosis of visceral leishmaniasis. J lmmlmologcal Methods 64:205\u2013214","journal-title":"J lmmlmologcal Methods"},{"issue":"1","key":"3266_CR11","first-page":"10","volume":"32","author":"A Gupta","year":"2008","unstructured":"Gupta A, Dixit AK, Dixit P (2008) Dot-ELISA in the diagnosis of parasitic diseases of animals. J Parasit Dis 32(1):10\u201314","journal-title":"J Parasit Dis"},{"key":"3266_CR12","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1016\/j.cca.2016.07.018","volume":"46","author":"K Zhang","year":"2016","unstructured":"Zhang K, Lin G, Han Y, Li J (2016) Serological diagnosis of toxoplasmosis and standardization. Clin Chim Acta 46:83\u201389","journal-title":"Clin Chim Acta"},{"key":"3266_CR13","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1007\/s00216-007-1631-2","volume":"390","author":"CA Marquette","year":"2008","unstructured":"Marquette CA, Blum LJ (2008) Electro-chemiluminescent biosensing. Anal Bioanal Chem 390:155\u2013168","journal-title":"Anal Bioanal Chem"},{"issue":"18","key":"3266_CR14","first-page":"20","volume":"20","author":"S Ali-Heydari","year":"2013","unstructured":"Ali-Heydari S, Keshavarz H, Shojaee S, Mohebali M (2013) Diagnosis of antigenic markers of acute toxoplasmosis by IgG avidity immunoblotting. Parasite 20(18):20\u201318","journal-title":"Parasite"},{"issue":"1","key":"3266_CR15","doi-asserted-by":"publisher","first-page":"142","DOI":"10.1016\/0003-2697(82)90677-7","volume":"119","author":"R Hawkes","year":"1982","unstructured":"Hawkes R, Niday E, Gordon J (1982) A dot-immunobinding assay for monoclonal and other antibodies. Anal Biochem 119(1):142\u2013147","journal-title":"Anal Biochem"},{"issue":"2\u20133","key":"3266_CR16","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1016\/0304-4017(88)90120-3","volume":"29","author":"MG Pappas","year":"1988","unstructured":"Pappas MG (1988) Recent applications of the Dot-ELISA in immunoparasitology. Vet Parasitol 29(2\u20133):105\u2013129","journal-title":"Vet Parasitol"},{"key":"3266_CR17","unstructured":"IBM Corp Released (2012) IBM SPSS statistics for windows, version 21.0. Armonk, NY: IBM Corp"},{"issue":"1","key":"3266_CR18","doi-asserted-by":"publisher","first-page":"159","DOI":"10.2307\/2529310","volume":"33","author":"JR Landis","year":"1977","unstructured":"Landis JR, Koch GG (1977) The measurement of observer agreement for categorical data. Biometrics 33(1):159\u2013174","journal-title":"Biometrics"},{"issue":"2","key":"3266_CR19","doi-asserted-by":"publisher","first-page":"469","DOI":"10.20506\/rst.17.2.1119","volume":"17","author":"RH Jacobson","year":"1998","unstructured":"Jacobson RH (1998) Validation of serological assays for diagnosis of infectious diseases. Rev Sci Tech 17(2):469\u2013526","journal-title":"Rev Sci Tech"},{"key":"3266_CR20","first-page":"133","volume":"107","author":"M Hayde","year":"1995","unstructured":"Hayde M, Salzer HR, Gittler G, Aspock H, Pollak A (1995) Microparticle enzyme immunoassay (MEIA) for toxoplasma specific immunoglobulin G in comparison to the Sabin-Feldman dye test. A pilot study. Wien Klin Wochenschr 107:133\u2013136","journal-title":"Wien Klin Wochenschr"},{"key":"3266_CR21","doi-asserted-by":"publisher","first-page":"164","DOI":"10.1128\/JCM.01435-08","volume":"47","author":"DC Kasper","year":"2009","unstructured":"Kasper DC, Prusa AR, Hayde M, Gerstl N, Pollak A, Herkner KR, Reiter-Reisacher R (2009) Evaluation of the Vitros ECiQ immunodiagnostic system for detection of anti-toxoplasma immunoglobulin G and immunoglobulin M antibodies for confirmatory testing for acute Toxoplasma gondii infection in pregnant women. J Clin Microbiol 47:164\u2013167","journal-title":"J Clin Microbiol"},{"key":"3266_CR22","doi-asserted-by":"publisher","first-page":"1570","DOI":"10.1128\/JCM.43.4.1570-1574.2005","volume":"43","author":"E Petersen","year":"2005","unstructured":"Petersen E, Borobio MV, El G et al (2005) European multicenter study of the LIAISON automated diagnostic system for determination of Toxoplasma gondii-specific immunoglobulin G (IgG) and IgM and the IgG avidity index. J Clin Microbiol 43:1570\u20131574","journal-title":"J Clin Microbiol"},{"key":"3266_CR23","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1016\/j.jmii.2011.01.005","volume":"44","author":"N Tekkesin","year":"2011","unstructured":"Tekkesin N, Keskin K, K\u0131l\u0131nc C, Orgen N, Molo K (2011) Detection of immunoglobulin G antibodies to Toxoplasma gondii: evaluation of two commercial immunoassay systems. J Microbiol Immunol Infect 44:21\u201326","journal-title":"J Microbiol Immunol Infect"},{"issue":"4","key":"3266_CR24","doi-asserted-by":"publisher","first-page":"352","DOI":"10.1016\/j.diagmicrobio.2010.07.011","volume":"68","author":"AR Prusa","year":"2010","unstructured":"Prusa AR, Hayde M, Unterasinger L, Pollak A, Herkner KR et al (2010) Evaluation of the Roche Elecsys Toxo IgG and IgM electrochemiluminescence immunoassay for the detection of gestational Toxoplasma infection. Diagn Microbiol Infect Dis 68(4):352\u2013357","journal-title":"Diagn Microbiol Infect Dis"},{"issue":"9","key":"3266_CR25","doi-asserted-by":"publisher","first-page":"943","DOI":"10.1586\/14787210.2013.825441","volume":"11","author":"JB Murat","year":"2013","unstructured":"Murat JB, Hidalgo HF, Brenier-Pinchart MP, Pelloux H (2013) Human toxoplasmosis: which biological diagnostic tests are best suited to which clinical situations? Expert Rev Anti-Infect Ther 11(9):943\u2013956","journal-title":"Expert Rev Anti-Infect Ther"},{"key":"3266_CR26","doi-asserted-by":"crossref","first-page":"3112","DOI":"10.1128\/JCM.35.12.3112-3115.1997","volume":"35","author":"M Wilson","year":"1997","unstructured":"Wilson M, Remington JS, Clavet C, Varney G, Press C, Ware D (1997) Evaluation of six commercial kits for detection of human immunoglobulin M antibodies to Toxoplasma gondii. J Clin Microbiol 35:3112\u20133115","journal-title":"J Clin Microbiol"},{"issue":"5","key":"3266_CR27","doi-asserted-by":"publisher","first-page":"471","DOI":"10.1258\/acb.2011.011013","volume":"48","author":"NR Syme","year":"2011","unstructured":"Syme NR, Toft AD, Stoddart M, Beckett GJ (2011) Clinical performance of the Roche cobas e411 automated assay system for thyrotropin-receptor antibodies for the diagnosis of Graves\u2019 disease. Ann Clin Biochem 48(5):471\u2013473","journal-title":"Ann Clin Biochem"},{"key":"3266_CR28","doi-asserted-by":"crossref","unstructured":"Selseleh M, Keshavarz H, Mohebali M, Shojaee S, et al (2012) Production and Evaluation of Toxoplasma gondii Recombinant GRA7 for Serodiagnosis of Human Infections. Korean J Parasitol 50(3):233\u2013238","DOI":"10.3347\/kjp.2012.50.3.233"},{"key":"3266_CR29","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1590\/S1676-24442003000200003","volume":"39","author":"PG Leser","year":"2003","unstructured":"Leser PG, de Assis Rocha LS, Moura MEG, Ferreira AW (2003) Comparison of semi-automatized assays for anti-T. gondii IgG detection in low-reactivity serum samples: importance of the results in patient counseling. J Bras Patol Med Lab 39:107\u2013110","journal-title":"J Bras Patol Med Lab"},{"issue":"2","key":"3266_CR30","doi-asserted-by":"publisher","first-page":"150","DOI":"10.1556\/1886.2015.00016","volume":"5","author":"P Meylan","year":"2015","unstructured":"Meylan P, Paris L, Liesenfeld O (2015) Multicenter evaluation of the Elecsys Toxo IgG and IgM tests for the diagnosis of infection with Toxoplasma gondii. Eur J Microbiol Immunol 5(2):150\u2013158","journal-title":"Eur J Microbiol Immunol"},{"key":"3266_CR31","doi-asserted-by":"publisher","first-page":"352","DOI":"10.1016\/j.diagmicrobio.2010.07.011","volume":"68","author":"AR Prusa","year":"2010","unstructured":"Prusa AR, Hayde M, Unterasinger L, Pollak A, Herkner KR et al (2010) Evaluation of the Roche Elecsys Toxo IgG and IgM electrochemiluminescence immunoassay for the detection of gestational Toxoplasma infection. Diagn Microbiol Infect Dis 68:352\u2013357","journal-title":"Diagn Microbiol Infect Dis"},{"key":"3266_CR32","first-page":"267","volume":"55","author":"J Helden Van","year":"2009","unstructured":"Van Helden J (2009) Performance of Elecsys toxo IgG and IgM immunoassays. Clin Lab 55:267\u2013273","journal-title":"Clin Lab"},{"key":"3266_CR33","doi-asserted-by":"publisher","first-page":"1838","DOI":"10.1128\/CVI.00333-12","volume":"19","author":"JB Murat","year":"2012","unstructured":"Murat JB, L\u2019Ollivier C, Fricker Hidalgo H, Franck J, Pelloux H et al (2012) Evaluation of the new Elecsys Toxo IgG avidity assay for toxoplasmosis and new insights into the interpretation of avidity results. Clin Vaccine Immunol 19:1838\u20131843","journal-title":"Clin Vaccine Immunol"},{"key":"3266_CR34","doi-asserted-by":"crossref","first-page":"2410","DOI":"10.1128\/JCM.27.11.2410-2413.1989","volume":"27","author":"LE Schaefer","year":"1989","unstructured":"Schaefer LE, Dyke JW, Meglio FD, Murray PR, Crafts W, Niles AC (1989) Evaluation of microparticle enzyme immunoassays for immunoglobulins G and M to rubella virus and Toxoplasma gondii on the Abbott IMx automated analyzer. J Clin Microbiol 27:2410\u20132413","journal-title":"J Clin Microbiol"},{"key":"3266_CR35","unstructured":"Selseleh M, Keshavarz H, Mohebali M, Shojaee S, Modarressi MH, Eshragian M (2012) Production and evaluation of Toxoplasma gondii recombinant surface antigen 1 (SAG1) for serodiagnosis of acute and chronic Toxoplasma infection in human sera. Iran J Parasitol 7:1\u20139"},{"key":"3266_CR36","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1016\/0304-4017(88)90119-7","volume":"29","author":"AM Tenter","year":"1988","unstructured":"Tenter AM (1988) Comparison of Dot-ELISA, ELISA and IFAT for the detection of IgG antibodies to Sarcocystis muris in experimentally infected and immunized mice. Vet Parasit 29:89\u2013104","journal-title":"Vet Parasit"},{"key":"3266_CR37","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1016\/S0304-4017(98)00166-6","volume":"79","author":"F Vercammen","year":"1998","unstructured":"Vercammen F, Berkvens D, Brandt J, Vansteenkiste W (1998) A sensitive and specific 30-min Dot-ELISA for the detection of anti-leishmania antibodies in the dog. Vet Parasitol 79:221\u2013228","journal-title":"Vet Parasitol"},{"key":"3266_CR38","doi-asserted-by":"publisher","first-page":"20","DOI":"10.4269\/ajtmh.1987.36.20","volume":"36","author":"L Wanduragala","year":"1987","unstructured":"Wanduragala L, Kakoma I, Clabaugh GW, Abeygunawardena I, Levy MG, Ristic M (1987) Development of dot-enzyme immunoassay for diagnosis of canine babesiosis. Am J Trop Med Hyg 36:20\u201321","journal-title":"Am J Trop Med Hyg"},{"key":"3266_CR39","unstructured":"Montenegro James S, Guillen AT, Toro M (1992) Dot-ELISA para el diagnostico serologico de la anaplasmosis y babesiosis bovina. Rev Ciesstifica FCV de Luz II:23\u201329"},{"key":"3266_CR40","first-page":"313","volume":"25","author":"E Camus","year":"1994","unstructured":"Camus E, Montenegro James S (1994) Bovine anaplasmosis and babesiosis in the lesser antilles: risk assessment of an unstable epidemiologic situation. Vet Res 25:313\u2013317","journal-title":"Vet Res"},{"key":"3266_CR41","doi-asserted-by":"publisher","first-page":"342","DOI":"10.1111\/j.1365-2915.1997.tb00420.x","volume":"11","author":"RC Mattioli","year":"1997","unstructured":"Mattioli RC, Janneh L, Corr N, Faye JA, Pandey US, Verhulet A (1997) Seasonal prevalence of ticks and tick transmitted haemoparasites in traditionally managed N\u2019Dama cattle with reference to strategic tick control in the Gambia. Med Vet Entomol 11:342\u2013348","journal-title":"Med Vet Entomol"},{"key":"3266_CR42","first-page":"28","volume":"6","author":"S Jafarpour Azami","year":"2011","unstructured":"Jafarpour Azami S, Keshavarz H, Rezaian M, Mohebali M, Shojaee S (2011) Rapid detection of Toxoplasma gondii antigen in experimentally infected mice by dot-ELISA. Iranian J Parasitol 6:28\u201333","journal-title":"Iranian J Parasitol"},{"key":"3266_CR43","first-page":"91","volume":"9","author":"MR Siavashi","year":"2005","unstructured":"Siavashi MR, Taherkhani H, Rezaei KR, Razavi Deligani MR, Assmar M (2005) Comparison of dot-ELISA and sandwich ELISA diagnostic tests in detection of human hydatidosis. Iran Biomed J 9:91\u201394","journal-title":"Iran Biomed J"}],"container-title":["European Journal of Clinical Microbiology &amp; Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s10096-018-3266-y\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10096-018-3266-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10096-018-3266-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T18:07:10Z","timestamp":1604254030000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s10096-018-3266-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,5,8]]},"references-count":43,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2018,8]]}},"alternative-id":["3266"],"URL":"https:\/\/doi.org\/10.1007\/s10096-018-3266-y","relation":{},"ISSN":["0934-9723","1435-4373"],"issn-type":[{"value":"0934-9723","type":"print"},{"value":"1435-4373","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,5,8]]},"assertion":[{"value":"27 March 2018","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 April 2018","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 May 2018","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"The study was approved by the Ethical Committee of Tehran University of Medical Sciences.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable (no individual person\u2019s data).","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The data that support the findings of this study are available on reasonable request to the corresponding author.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Availability of data and material"}},{"value":"The authors declare that they have no conflict of interest.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}