{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T04:07:43Z","timestamp":1793333263818,"version":"4.5.0"},"reference-count":16,"publisher":"Springer Science and Business Media LLC","issue":"9","license":[{"start":{"date-parts":[[2019,5,22]],"date-time":"2019-05-22T00:00:00Z","timestamp":1558483200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Eur J Clin Microbiol Infect Dis"],"published-print":{"date-parts":[[2019,9]]},"DOI":"10.1007\/s10096-019-03595-y","type":"journal-article","created":{"date-parts":[[2019,5,23]],"date-time":"2019-05-23T01:23:14Z","timestamp":1558574594000},"page":"1665-1670","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Evaluation of the Alfred AST\u00ae system for rapid antimicrobial susceptibility testing directly from positive blood cultures"],"prefix":"10.1007","volume":"38","author":[{"given":"Carlos","family":"S\u00e1nchez-Carrillo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula","family":"Pescador","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa","family":"Ricote","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Fuentes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Losada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Candela","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilia","family":"Cercenado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,5,22]]},"reference":[{"key":"3595_CR1","volume-title":"Cumitech 1C: blood cultures IV","author":"EJ Baron","year":"2005","unstructured":"Baron EJ, Weinstein MP, Dunne WM, Yagupsky P, Welch DF, Wilson DM (2005) In: Coordinating ed., Baron EJ (eds) Cumitech 1C: blood cultures IV. American Society for Microbiology, Washington, DC"},{"key":"3595_CR2","doi-asserted-by":"publisher","first-page":"513","DOI":"10.1111\/1469-0691.12180","volume":"19","author":"TJ Kirn","year":"2013","unstructured":"Kirn TJ, Weinstein MP (2013) Update on blood cultures: how to obtain, process, report, and interpret. Clin Microbiol Infect 19:513\u2013520","journal-title":"Clin Microbiol Infect"},{"key":"3595_CR3","doi-asserted-by":"publisher","first-page":"495","DOI":"10.1128\/JCM.41.1.495-497.2003","volume":"41","author":"EL Munson","year":"2003","unstructured":"Munson EL, Diekema DJ, Beekmann SE, Chapin KC, Doern GV (2003) Detection and treatment of bloodstream infection: laboratory reporting and antimicrobial management. J Clin Microbiol 41:495\u2013497","journal-title":"J Clin Microbiol"},{"key":"3595_CR4","doi-asserted-by":"publisher","first-page":"1237","DOI":"10.1093\/cid\/cit498","volume":"57","author":"MH Angela","year":"2013","unstructured":"Angela MH, Newton D, Kunapuli A, Gandhi TN, Washer LL, Isip J et al (2013) Impact of rapid organism identification via matrix-assisted laser desorption\/ionization time-of-flight combined with antimicrobial stewardship team intervention in adult patients with bacteremia and candidemia. Clin Infect Dis 57:1237\u20131245","journal-title":"Clin Infect Dis"},{"key":"3595_CR5","doi-asserted-by":"publisher","first-page":"1076","DOI":"10.1086\/656623","volume":"51","author":"KA Bauer","year":"2010","unstructured":"Bauer KA, West JE, Balada-Llasat JM, Pancholi P, Stevenson KB, Goff DA (2010) An antimicrobial stewardship program\u2019s impact with rapid polymerase chain reaction methicillin-resistant Staphylococcus aureus\/S. aureus blood culture test in patients with S. aureus bacteremia. Clin Infect Dis 51:1076\u20131080","journal-title":"Clin Infect Dis"},{"key":"3595_CR6","doi-asserted-by":"publisher","first-page":"1247","DOI":"10.5858\/arpa.2012-0651-OA","volume":"137","author":"KK Perez","year":"2013","unstructured":"Perez KK, Olsen RJ, Musick WL, Cernoch PL, Davis JR, Land GA et al (2013) Integrating rapid pathogen identification and antimicrobial stewardship significantly decreases hospital costs. Arch Pathol Lab Med 137:1247\u20131254","journal-title":"Arch Pathol Lab Med"},{"key":"3595_CR7","first-page":"3.4.1.1","volume-title":"Clinical microbiology procedures handbook","author":"MK York","year":"2007","unstructured":"York MK, Henry M, Gilligan P (2007) Blood cultures. Clinical detection and interpretation. In: Garc\u00eda LS (ed) Clinical microbiology procedures handbook, Third edn. ASM Press, Washington, DC, pp 3.4.1.1\u20133.4.1.20","edition":"Third"},{"key":"3595_CR8","volume-title":"Cumitech 31a, verification and validation procedures in the clinical microbiology laboratory","author":"RB Clark","year":"2009","unstructured":"Clark RB, Lewinski MA, Loeffelholz MJ, Tibbetts RJ (2009) In: Coordinating ed., Sharp SE (eds) Cumitech 31a, verification and validation procedures in the clinical microbiology laboratory. American Society for Microbiology, Washington, DC"},{"key":"3595_CR9","doi-asserted-by":"publisher","first-page":"2013","DOI":"10.1128\/JCM.00221-09","volume":"47","author":"JM Swenson","year":"2009","unstructured":"Swenson JM, Anderson KF, Lonsway DR, Thompson A, McAllister SK, Limbago BM et al (2009) Accuracy of commercial and reference susceptibility testing methods for detecting vancomycin-intermediate Staphylococcus aureus. J Clin Microbiol 47:2013\u20132017","journal-title":"J Clin Microbiol"},{"key":"3595_CR10","doi-asserted-by":"publisher","first-page":"2272","DOI":"10.1128\/JCM.02215-10","volume":"49","author":"MC Kruzel","year":"2011","unstructured":"Kruzel MC, Lewis CT, Welsh KJ, Lewis EM, Dundas NE, Mohr JF et al (2011) Determination of vancomycin and daptomycin MICs by different testing methods for methicillin-resistant Staphylococcus aureus. J Clin Microbiol 49:2272\u20132273","journal-title":"J Clin Microbiol"},{"key":"3595_CR11","doi-asserted-by":"publisher","first-page":"2077","DOI":"10.1128\/JCM.00448-13","volume":"51","author":"MJ Rybak","year":"2013","unstructured":"Rybak MJ, Vidaillac C, Sader HS, Rhomberg PR, Salimnia H, Briski LE et al (2013) Evaluation of vancomycin susceptibility testing for methicillin-resistant Staphylococcus aureus: comparison of Etest and three automated testing methods. J Clin Microbiol 51:2077\u20132081","journal-title":"J Clin Microbiol"},{"key":"3595_CR12","doi-asserted-by":"publisher","first-page":"421","DOI":"10.1111\/1469-0691.12455","volume":"20","author":"B Rodr\u00edguez-S\u00e1nchez","year":"2014","unstructured":"Rodr\u00edguez-S\u00e1nchez B, S\u00e1nchez-Carrillo C, Ruiz A, Mar\u00edn M, Cercenado E, Rodr\u00edguez-Cr\u00e9ixems M et al (2014) Direct identification of pathogens from positive blood cultures using matrix-assisted laser desorption-ionization time-of-flight mass spectrometry. Clin Microbiolol Infect 20:421\u2013427","journal-title":"Clin Microbiolol Infect"},{"key":"3595_CR13","first-page":"513","volume":"65","author":"MP Romero-G\u00f3mez","year":"2012","unstructured":"Romero-G\u00f3mez MP, G\u00f3mez-Gil R, Pa\u00f1o-Pardo JR, Mignorance J (2012) Identification and susceptibility testing of microorganism by direct inoculation from positive blood culture bottles by combining MALDI-TOF and Vitek-2 compact is rapid and effective. J Inf Secur 65:513\u2013520","journal-title":"J Inf Secur"},{"key":"3595_CR14","doi-asserted-by":"publisher","first-page":"3988","DOI":"10.1128\/JCM.01889-13","volume":"51","author":"SG Beal","year":"2013","unstructured":"Beal SG, Ciurca J, Smith G, John J, Lee F, Doern CD et al (2013) Evaluation of the Nanosphere Verigene gram-positive blood culture assay with the VersaTREK blood culture system and assessment of possible impact on selected patients. J Clin Microbiol 51:3988\u20133992","journal-title":"J Clin Microbiol"},{"key":"3595_CR15","doi-asserted-by":"publisher","first-page":"1588","DOI":"10.1128\/AAC.04259-14","volume":"59","author":"JT Bork","year":"2015","unstructured":"Bork JT, Leekha S, Heil EL, Zhao L, Badamas R, Johnson JK (2015) Rapid testing using the Verigene gram-negative blood culture nucleic acid test in combination with antimicrobial stewardship intervention against gram-negative bacteremia. Antimicrob Agents Chemother 59:1588\u20131595","journal-title":"Antimicrob Agents Chemother"},{"key":"3595_CR16","doi-asserted-by":"publisher","first-page":"487","DOI":"10.1007\/s10096-014-2252-2","volume":"34","author":"C Ward","year":"2015","unstructured":"Ward C, Stocker K, Begum J, Wade P, Ebrahimsa U, Goldenberg SD (2015) Performance evaluation of the Verigene\u00ae (Nanosphere) and FilmArray\u00ae (BioFire\u00ae) molecular assays for identification of causative organisms in bacterial bloodstream infections. Eur J Clin Microbiol Infect Dis 34:487\u2013496","journal-title":"Eur J Clin Microbiol Infect Dis"}],"container-title":["European Journal of Clinical Microbiology &amp; Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10096-019-03595-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s10096-019-03595-y\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s10096-019-03595-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,8,29]],"date-time":"2019-08-29T10:33:08Z","timestamp":1567074788000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s10096-019-03595-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,5,22]]},"references-count":16,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2019,9]]}},"alternative-id":["3595"],"URL":"https:\/\/doi.org\/10.1007\/s10096-019-03595-y","relation":{},"ISSN":["0934-9723","1435-4373"],"issn-type":[{"value":"0934-9723","type":"print"},{"value":"1435-4373","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,5,22]]},"assertion":[{"value":"13 March 2019","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 May 2019","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 May 2019","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"The authors declare that they do not have conflicts of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"Informed consent was obtained from all individual participants included in the study.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Informed consent"}},{"value":"All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}}]}}