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Prevalence is generally underestimated since many infections are asymptomatic, and traditional diagnostic tests based on parasitological examination of stool samples are not adequately sensitive. Serological tests are useful and supportive but are still only available in a reference research setting. We made an immunochromatographic test (ICT) kit for rapid serodiagnosis of human strongyloidiasis. The antigen used in the ICT kit was extracted from larvae of <jats:italic>Strongyloides stercoralis<\/jats:italic>. Diagnostic efficacy of the kit was evaluated using human serum samples from strongyloidiasis patients, healthy persons, and those with other parasitoses. When using a cutoff level of 0.5 or above, the diagnostic sensitivity, specificity, and positive and negative predictive values at the prevalence of infection of 34.4%, were 93.3%, 83.7%, 76.7%, and 95.6%, respectively. This ICT kit is easy to use at the point-of-care and a result can be obtained in 15\u00a0min. Sophisticated instruments and highly trained staff are not required. It can be used in several diagnostic and public-health settings, e.g., prevalence surveys in endemic areas, confirmation and monitoring of cure post-treatment, diagnosis and screening of infected but asymptomatic individuals, and populations \u201cat risk\u201d for hyperinfection syndrome or disseminated strongyloidiasis if they are given immunosuppressive treatment for other conditions.<\/jats:p>","DOI":"10.1007\/s10096-019-03745-2","type":"journal-article","created":{"date-parts":[[2019,11,22]],"date-time":"2019-11-22T23:02:22Z","timestamp":1574463742000},"page":"465-470","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Development of immunochromatographic device as a point-of-care tool for serodiagnosis of human strongyloidiasis cases"],"prefix":"10.1007","volume":"39","author":[{"given":"Lakkhana","family":"Sadaow","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oranuch","family":"Sanpool","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rutchanee","family":"Rodpai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patcharaporn","family":"Boonroumkaew","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanchai","family":"Maleewong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2138-8783","authenticated-orcid":false,"given":"Pewpan M.","family":"Intapan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,11,22]]},"reference":[{"key":"3745_CR1","doi-asserted-by":"publisher","first-page":"1040","DOI":"10.1086\/322707","volume":"33","author":"AA Siddiqui","year":"2001","unstructured":"Siddiqui AA, Berk SL (2001) Diagnosis of Strongyloides stercoralis infection. 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