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The increasing antibiotic resistance among<jats:italic>Staphylococcus epidermidis<\/jats:italic>is an emerging problem worldwide. In particular, the linezolid-resistant<jats:italic>S. epidermidis<\/jats:italic>(LRSE) strains are observed in Europe since 2014. The aim of our study was to genetically characterize 11 LRSE isolates, recovered mostly from blood in the University Children\u2019s Hospital in Krakow, Poland, between 2015 and 2017. For identification of the isolates at the species level, we used 16S rRNA sequencing and RFLP of the<jats:italic>saoC<\/jats:italic>gene. Isolates were characterized phenotypically by determining their antimicrobial resistance patterns and using molecular methods such as PFGE, MLST, SCC<jats:italic>mec<\/jats:italic>typing, detection of the<jats:italic>ica<\/jats:italic>operon, and analysis of antimicrobial resistance determinants. All isolates were multidrug-resistant, including resistance to methicillin, and exhibited so-called PhLOPS<jats:sub>A<\/jats:sub>phenotype. In PFGE, all isolates (excluding one from a catheter) represented identical patterns, were identified as ST2, and harbored the<jats:italic>ica<\/jats:italic>operon, responsible for biofilm formation. Linezolid resistance was associated with acquisition of A157R mutation in the ribosomal protein L3 and the presence of<jats:italic>cfr<\/jats:italic>gene. All isolates revealed new SCC<jats:italic>mec<\/jats:italic>cassette element composition. Recently, pediatric patients with serious staphylococcal infections are often treated with linezolid. The increasing linezolid resistance in bacterial strains becomes a real threat for patients, and monitoring such infections combined with surveillance and infection prevention programs is very important to decrease number of linezolid-resistant staphylococcal strains.<\/jats:p>","DOI":"10.1007\/s10096-020-03893-w","type":"journal-article","created":{"date-parts":[[2020,7,8]],"date-time":"2020-07-08T10:03:43Z","timestamp":1594202623000},"page":"1717-1725","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Emergence of linezolid-resistant Staphylococcus epidermidis in the tertiary children\u2019s hospital in Cracow, Poland"],"prefix":"10.1007","volume":"39","author":[{"given":"Maja","family":"Kosecka-Strojek","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ewa","family":"Sadowy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iwona","family":"Gawryszewska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Klepacka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomasz","family":"Tomasik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michal","family":"Michalik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Waleria","family":"Hryniewicz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacek","family":"Miedzobrodzki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,4,29]]},"reference":[{"key":"3893_CR1","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1016\/B978-0-12-813547-1.00002-9","volume-title":"Pet-to-man travelling staphylococci: a world in progress","author":"M Kosecka-Strojek","year":"2018","unstructured":"Kosecka-Strojek M, Buda A, Miedzobrodzki J (2018) Staphylococcal ecology and epidemiology. 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