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We compared the results of the Allplex\u2122 2019-nCoV assay with those of the Simplexa\u2122 COVID-19 Direct assay and the Quanty COVID-19 assay, respectively, all performed on 125 nasal\/oropharyngeal swab samples of patients with COVID-19 suspicion. Fifty-four samples were positive, and 71 were negative with the Allplex\u2122 assay, whereas 47 of 54 samples were also positive with the Simplexa\u2122 assay. The Quanty assay detected 55 positive samples, including the 54 positive samples with the Allplex\u2122 assay and 1 sample that was Allplex\u2122 negative but Simplexa\u2122 positive. Using a consensus result criterion as the reference standard allowed to resolve the eight samples with discordant results (one Allplex\u2122 negative and seven Simplexa\u2122 negative) as truly false negative. Interestingly, a Spearman\u2019s negative association was found between the viral RNA loads quantified by the Quanty assay and the <jats:italic>C<\/jats:italic><jats:sub><jats:italic>T<\/jats:italic><\/jats:sub> values of RT PCRs performed with either the Allplex\u2122 assay or the Simplexa\u2122 assay. However, the strength of this association was higher for the Allplex\u2122 assay (N gene, <jats:italic>\u03c1<\/jats:italic>\u2009=\u2009\u2212\u20090.92; RdRP gene, <jats:italic>\u03c1<\/jats:italic>\u2009=\u2009\u2212\u20090.91) than for the Simplexa\u2122 assay (ORF1ab gene, <jats:italic>\u03c1<\/jats:italic>\u2009=\u2009\u2212\u20090.65; S gene, <jats:italic>\u03c1<\/jats:italic>\u2009=\u2009\u2212\u20090.80). The Allplex\u2122 2019-nCoV, the Simplexa\u2122 COVID-19 Direct, and the Quanty COVID-19 assays yielded comparable results. However, the role these assays might play in future clinical practice warrants larger comparison studies.<\/jats:p>","DOI":"10.1007\/s10096-020-04025-0","type":"journal-article","created":{"date-parts":[[2020,9,4]],"date-time":"2020-09-04T02:02:36Z","timestamp":1599184956000},"page":"269-277","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Evaluation of three commercial assays for SARS-CoV-2 molecular detection in upper respiratory tract samples"],"prefix":"10.1007","volume":"40","author":[{"given":"Flora Marzia","family":"Liotti","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giulia","family":"Menchinelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simona","family":"Marchetti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grazia Angela","family":"Morandotti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9780-7059","authenticated-orcid":false,"given":"Maurizio","family":"Sanguinetti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brunella","family":"Posteraro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola","family":"Cattani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,9,4]]},"reference":[{"key":"4025_CR1","doi-asserted-by":"publisher","first-page":"727","DOI":"10.1056\/NEJMoa2001017","volume":"382","author":"N Zhu","year":"2020","unstructured":"Zhu N, Zhang D, Wang W, Li X, Yang B, Song J, Zhao X, Huang B, Shi W, Lu R, Niu P, Zhan F, Ma X, Wang D, Xu W, Wu G, Gao GF, Tan W, China Novel Coronavirus Investigating and Research Team (2020) A novel coronavirus from patients with pneumonia in China, 2019. 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