{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T07:05:27Z","timestamp":1790665527827,"version":"4.1.0"},"reference-count":26,"publisher":"Springer Science and Business Media LLC","issue":"7","license":[{"start":{"date-parts":[[2021,1,30]],"date-time":"2021-01-30T00:00:00Z","timestamp":1611964800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2021,1,30]],"date-time":"2021-01-30T00:00:00Z","timestamp":1611964800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Eur J Clin Microbiol Infect Dis"],"published-print":{"date-parts":[[2021,7]]},"DOI":"10.1007\/s10096-021-04170-0","type":"journal-article","created":{"date-parts":[[2021,1,30]],"date-time":"2021-01-30T00:04:11Z","timestamp":1611965051000},"page":"1413-1418","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Clinical evaluation of commercial PCR assays for antimicrobal resistance in Mycoplasma genitalium and estimation of resistance-mediated mutation prevalence in Moscow and Moscow region"],"prefix":"10.1007","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4556-7513","authenticated-orcid":false,"given":"Elizaveta Dmitrievna","family":"Shedko","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1544-0730","authenticated-orcid":false,"given":"Guzel Anvarovna","family":"Khayrullina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0536-2874","authenticated-orcid":false,"given":"Elena Nikolaevna","family":"Goloveshkina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4228-9044","authenticated-orcid":false,"given":"Vasiliy Gennadevich","family":"Akimkin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,1,30]]},"reference":[{"key":"4170_CR1","doi-asserted-by":"publisher","first-page":"498","DOI":"10.1128\/CMR.00006-11","volume":"24","author":"D Taylor-Robinson","year":"2011","unstructured":"Taylor-Robinson D, Jensen JS (2011) Mycoplasma genitalium: from chrysalis to multicolored butterfly. Clin Microbiol Rev 24:498\u2013514. https:\/\/doi.org\/10.1128\/CMR.00006-11","journal-title":"Clin Microbiol Rev"},{"key":"4170_CR2","doi-asserted-by":"publisher","first-page":"1288","DOI":"10.1016\/S0140-6736(81)92461-2","volume":"317","author":"J Tully","year":"1981","unstructured":"Tully J, Cole R, Taylor-Robinson D, Rose D (1981) A newly discovered mycoplasma in the human urogenital tract. Lancet 317:1288\u20131291. https:\/\/doi.org\/10.1016\/S0140-6736(81)92461-2","journal-title":"Lancet"},{"key":"4170_CR3","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1136\/sextrans-2017-053384","volume":"94","author":"L Baumann","year":"2018","unstructured":"Baumann L, Cina M, Egli-Gany D, Goutaki M, Halbeisen FS, Lohrer G-R et al (2018) Prevalence of Mycoplasma genitalium in different population groups: systematic review andmeta-analysis. Sex Transm Infect 94:255\u2013262. https:\/\/doi.org\/10.1136\/sextrans-2017-053384","journal-title":"Sex Transm Infect"},{"key":"4170_CR4","doi-asserted-by":"publisher","first-page":"418","DOI":"10.1093\/cid\/civ312","volume":"61","author":"R Lis","year":"2015","unstructured":"Lis R, Rowhani-Rahbar A, Manhart LE (2015) Mycoplasma genitalium infection and female reproductive tract disease: a meta-analysis. Clin Infect Dis 61:418\u2013426. https:\/\/doi.org\/10.1093\/cid\/civ312","journal-title":"Clin Infect Dis"},{"key":"4170_CR5","doi-asserted-by":"publisher","first-page":"611","DOI":"10.1097\/QAD.0b013e328323da3e","volume":"23","author":"S Napierala Mavedzenge","year":"2009","unstructured":"Napierala Mavedzenge S, Weiss HA (2009) Association of Mycoplasma genitalium and HIV infection: a systematic review and meta-analysis. AIDS 23:611\u2013620. https:\/\/doi.org\/10.1097\/QAD.0b013e328323da3e","journal-title":"AIDS"},{"key":"4170_CR6","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1093\/ofid\/ofaa291","volume":"1","author":"M Doyle","year":"2020","unstructured":"Doyle M, Vodstrcil LA, Plummer EL, Aguirre I, Fairley CK, Bradshaw CS (2020) Nonquinolone Options for the treatment of Mycoplasma genitalium in the era of increased resistance. Open Forum Infect Dis 1:7. https:\/\/doi.org\/10.1093\/ofid\/ofaa291","journal-title":"Open Forum Infect Dis"},{"key":"4170_CR7","doi-asserted-by":"publisher","first-page":"S406","DOI":"10.1093\/infdis\/jix104","volume":"216","author":"CA Gaydos","year":"2017","unstructured":"Gaydos CA (2017) Mycoplasma genitalium: accurate diagnosis is necessary for adequate treatment. J Infect Dis 216:S406\u2013S411. https:\/\/doi.org\/10.1093\/infdis\/jix104","journal-title":"J Infect Dis"},{"key":"4170_CR8","doi-asserted-by":"publisher","first-page":"1650","DOI":"10.1111\/jdv.13849","volume":"30","author":"JS Jensen","year":"2016","unstructured":"Jensen JS, Cusini M, Gomberg M, Moi H (2016) 2016 European guideline on Mycoplasma genitalium infections. J Eur Acad Dermatol Venereol 30:1650\u20131656. https:\/\/doi.org\/10.1111\/jdv.13849","journal-title":"J Eur Acad Dermatol Venereol"},{"key":"4170_CR9","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1136\/sextrans-2018-053831","volume":"95","author":"S Soni","year":"2019","unstructured":"Soni S, Horner PJ (2019) Launch of the BASHH guideline for the management of M. genitalium in adults. Sex Transm Infect 95:237\u2013237. https:\/\/doi.org\/10.1136\/sextrans-2018-053831","journal-title":"Sex Transm Infect"},{"key":"4170_CR10","doi-asserted-by":"publisher","first-page":"S382","DOI":"10.1093\/infdis\/jix172","volume":"216","author":"CL McGowin","year":"2017","unstructured":"McGowin CL, Totten PA (2017) The unique microbiology and molecular pathogenesis of Mycoplasma genitalium. J Infect Dis 216:S382\u2013S388. https:\/\/doi.org\/10.1093\/infdis\/jix172","journal-title":"J Infect Dis"},{"key":"4170_CR11","doi-asserted-by":"publisher","first-page":"2967","DOI":"10.1111\/bph.13936","volume":"174","author":"GP Dinos","year":"2017","unstructured":"Dinos GP (2017) The macrolide antibiotic renaissance. Br J Pharmacol 174:2967\u20132983. https:\/\/doi.org\/10.1111\/bph.13936","journal-title":"Br J Pharmacol"},{"key":"4170_CR12","doi-asserted-by":"publisher","first-page":"1546","DOI":"10.1086\/593188","volume":"47","author":"JS Jensen","year":"2008","unstructured":"Jensen JS, Bradshaw CS, Tabrizi SN, Fairley CK, Hamasuna R (2008) Azithromycin treatment failure in Mycoplasma genitalium\u2013positive patients with nongonococcal urethritis is associated with Induced macrolide resistance. Clin Infect Dis 47:1546\u20131553. https:\/\/doi.org\/10.1086\/593188","journal-title":"Clin Infect Dis"},{"key":"4170_CR13","doi-asserted-by":"publisher","first-page":"7","DOI":"10.17116\/klinderma2020190117","volume":"19","author":"IV Romanova","year":"2020","unstructured":"Romanova IV, Kisina VI, Khayrullina GA, Frigo NV, Zhukova OV, Gushchin AE (2020) The prevalence and type of mutations of in dermatovenereological patients from the Moscow region for 2014\u20132018. Klin dermatologiya i Venerol 19:7. https:\/\/doi.org\/10.17116\/klinderma2020190117","journal-title":"Klin dermatologiya i Venerol"},{"key":"4170_CR14","doi-asserted-by":"publisher","first-page":"a025395","DOI":"10.1101\/cshperspect.a025395","volume":"6","author":"C Fyfe","year":"2016","unstructured":"Fyfe C, Grossman TH, Kerstein K, Sutcliffe J (2016) Resistance to macrolide antibiotics in public health pathogens. Cold Spring Harb Perspect Med 6:a025395. https:\/\/doi.org\/10.1101\/cshperspect.a025395","journal-title":"Cold Spring Harb Perspect Med"},{"key":"4170_CR15","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1186\/s12879-015-0781-7","volume":"15","author":"A Guschin","year":"2015","unstructured":"Guschin A, Ryzhikh P, Rumyantseva T, Gomberg M, Unemo M (2015) Treatment efficacy, treatment failures and selection of macrolide resistance in patients with high load of Mycoplasma genitalium during treatment of male urethritis with josamycin. BMC Infect Dis 15:40. https:\/\/doi.org\/10.1186\/s12879-015-0781-7","journal-title":"BMC Infect Dis"},{"key":"4170_CR16","doi-asserted-by":"publisher","first-page":"S73","DOI":"10.1016\/j.survophthal.2004.01.005","volume":"49","author":"JM Blondeau","year":"2004","unstructured":"Blondeau JM (2004) Fluoroquinolones: mechanism of action, classification, and development of resistance. Surv Ophthalmol 49:S73\u2013S78. https:\/\/doi.org\/10.1016\/j.survophthal.2004.01.005","journal-title":"Surv Ophthalmol"},{"key":"4170_CR17","doi-asserted-by":"publisher","unstructured":"Murray GL, Bodiyabadu K, Danielewski J, Garland SM, Machalek DA, Fairley CK et al (2019) The parC mutation G248T (S83I), and concurrent gyrA mutations, are associated with moxifloxacin and sitafloxacin treatment failure for Mycoplasma genitalium. J Infect Dis. https:\/\/doi.org\/10.1093\/infdis\/jiz550","DOI":"10.1093\/infdis\/jiz550"},{"key":"4170_CR18","doi-asserted-by":"publisher","first-page":"e0198355","DOI":"10.1371\/journal.pone.0198355","volume":"13","author":"R Hamasuna","year":"2018","unstructured":"Hamasuna R, Le PT, Kutsuna S, Furubayashi K, Matsumoto M, Ohmagari N et al (2018) Mutations in ParC and GyrA of moxifloxacin-resistant and susceptible Mycoplasma genitalium strains. PLoS One 13:e0198355. https:\/\/doi.org\/10.1371\/journal.pone.0198355","journal-title":"PLoS One"},{"key":"4170_CR19","doi-asserted-by":"publisher","first-page":"950","DOI":"10.1186\/s12879-020-05659-3","volume":"20","author":"W Ke","year":"2020","unstructured":"Ke W, Li D, Tso LS, Wei R, Lan Y, Chen Z et al (2020) Macrolide and fluoroquinolone associated mutations in Mycoplasma genitalium in a retrospective study of male and female patients seeking care at a STI Clinic in Guangzhou, China, 2016-2018. BMC Infect Dis 20:950. https:\/\/doi.org\/10.1186\/s12879-020-05659-3","journal-title":"BMC Infect Dis"},{"key":"4170_CR20","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1016\/j.eimc.2019.06.008","volume":"38","author":"M Fern\u00e1ndez-Huerta","year":"2020","unstructured":"Fern\u00e1ndez-Huerta M, Vall M, Fern\u00e1ndez-Naval C, Barber\u00e1 M-J, Arando M, L\u00f3pez L et al (2020) Mycoplasma genitalium macrolide resistance update: Rate among a 2016\u20132017 cohort of patients in Barcelona. Spain Enferm Infecc Microbiol Clin 38:99\u2013104. https:\/\/doi.org\/10.1016\/j.eimc.2019.06.008","journal-title":"Spain Enferm Infecc Microbiol Clin"},{"key":"4170_CR21","doi-asserted-by":"publisher","first-page":"742","DOI":"10.1093\/jac\/48.5.742","volume":"48","author":"T Deguchi","year":"2001","unstructured":"Deguchi T (2001) Analysis of the gyrA and parC genes ofMycoplasma genitaliumdetected in first-pass urine of men with non-gonococcal urethritis before and after fluoroquinolone treatment. J Antimicrob Chemother 48:742\u2013744. https:\/\/doi.org\/10.1093\/jac\/48.5.742","journal-title":"J Antimicrob Chemother"},{"key":"4170_CR22","doi-asserted-by":"publisher","first-page":"1645","DOI":"10.2147\/IDR.S173867","volume":"11","author":"B Aslam","year":"2018","unstructured":"Aslam B, Wang W, Arshad MI, Khurshid M, Muzammil S, Rasool MH et al (2018) Antibiotic resistance: a rundown of a global crisis. Infect Drug Resist 11:1645\u20131658. https:\/\/doi.org\/10.2147\/IDR.S173867","journal-title":"Infect Drug Resist"},{"key":"4170_CR23","doi-asserted-by":"publisher","first-page":"1915","DOI":"10.1128\/JCM.02312-16","volume":"55","author":"SN Tabrizi","year":"2017","unstructured":"Tabrizi SN, Su J, Bradshaw CS, Fairley CK, Walker S, Tan LY et al (2017) Prospective evaluation of ResistancePlus MG, a new multiplex quantitative PCR assay for detection of Mycoplasma genitalium and macrolide resistance. J Clin Microbiol 55:1915\u20131919. https:\/\/doi.org\/10.1128\/JCM.02312-16","journal-title":"J Clin Microbiol"},{"key":"4170_CR24","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1128\/JCM.01245-17","volume":"1","author":"JP Su","year":"2017","unstructured":"Su JP, Tan LY, Garland SM, Tabrizi SN, Mokany E, Walker S et al (2017) Evaluation of the SpeeDx ResistancePlus MG diagnostic test for Mycoplasma genitalium on the Applied Biosystems 7500 Fast Quantitative PCR Platform. J Clin Microbiol 1:56. https:\/\/doi.org\/10.1128\/JCM.01245-17","journal-title":"J Clin Microbiol"},{"key":"4170_CR25","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1128\/JCM.01478-19","volume":"4","author":"C Le Roy","year":"2019","unstructured":"Le Roy C, B\u00e9b\u00e9ar C, Pereyre S (2019) Clinical evaluation of three commercial PCR assays for the detection of macrolide resistance in Mycoplasma genitalium. J Clin Microbiol 4:58. https:\/\/doi.org\/10.1128\/JCM.01478-19","journal-title":"J Clin Microbiol"},{"key":"4170_CR26","doi-asserted-by":"publisher","first-page":"e0175763","DOI":"10.1371\/journal.pone.0175763","volume":"12","author":"E Shipitsyna","year":"2017","unstructured":"Shipitsyna E, Rumyantseva T, Golparian D, Khayrullina G, Lagos AC, Edelstein I et al (2017) Prevalence of macrolide and fluoroquinolone resistance-mediating mutations in Mycoplasma genitalium in five cities in Russia and Estonia. PLoS One 12:e0175763. https:\/\/doi.org\/10.1371\/journal.pone.0175763","journal-title":"PLoS One"}],"container-title":["European Journal of Clinical Microbiology & Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10096-021-04170-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10096-021-04170-0\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10096-021-04170-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,6,15]],"date-time":"2021-06-15T13:05:26Z","timestamp":1623762326000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10096-021-04170-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1,30]]},"references-count":26,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2021,7]]}},"alternative-id":["4170"],"URL":"https:\/\/doi.org\/10.1007\/s10096-021-04170-0","relation":{},"ISSN":["0934-9723","1435-4373"],"issn-type":[{"value":"0934-9723","type":"print"},{"value":"1435-4373","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1,30]]},"assertion":[{"value":"28 October 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 January 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"30 January 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Retrospective analysis of previously obtained samples and cross-sectional examination does not require ethical review.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no conflict of interest.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}