{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:24:44Z","timestamp":1772119484099,"version":"3.50.1"},"reference-count":34,"publisher":"Springer Science and Business Media LLC","issue":"9","license":[{"start":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T00:00:00Z","timestamp":1617235200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T00:00:00Z","timestamp":1617235200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Eur J Clin Microbiol Infect Dis"],"published-print":{"date-parts":[[2021,9]]},"DOI":"10.1007\/s10096-021-04236-z","type":"journal-article","created":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T03:04:53Z","timestamp":1617246293000},"page":"1851-1861","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Exploratory development of PCR-fluorescent probes in rapid detection of mutations associated with extensively drug-resistant tuberculosis"],"prefix":"10.1007","volume":"40","author":[{"given":"Jianqin","family":"Liang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huiru","family":"An","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuqin","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guangxin","family":"Xiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingying","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wanli","family":"Xing","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0333-890X","authenticated-orcid":false,"given":"Wenping","family":"Gong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,4,1]]},"reference":[{"key":"4236_CR1","first-page":"1","volume-title":"Global tuberculosis report 2019","author":"WHO","year":"2019","unstructured":"WHO (2019) Global tuberculosis report 2019. World Health Organization, Geneva, pp 1\u2013297"},{"key":"4236_CR2","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1186\/s13756-017-0270-4","volume":"6","author":"KM Akshaya","year":"2017","unstructured":"Akshaya KM, Shewade HD, Aslesh OP, Nagaraja SB, Nirgude AS, Singarajipura A, Jacob AG (2017) \u201cWho has to do it at the end of the day? Programme officials or hospital authorities?\u201d Airborne infection control at drug resistant tuberculosis (DR-TB) centres of Karnataka, India: a mixed-methods study. Antimicrob Resist Infect Control 6:111","journal-title":"Antimicrob Resist Infect Control"},{"issue":"3","key":"4236_CR3","doi-asserted-by":"publisher","first-page":"154","DOI":"10.1016\/j.cmi.2016.10.022","volume":"23","author":"T Sch\u00f6n","year":"2017","unstructured":"Sch\u00f6n T, Miotto P, K\u00f6ser CU, Viveiros M, B\u00f6ttger E, Cambau E (2017) Mycobacterium tuberculosis drug-resistance testing: challenges, recent developments and perspectives. Clin Microbiol Infect: Off Publ Eur Soc Clin Microbiol Infect Dis 23(3):154\u2013160","journal-title":"Clin Microbiol Infect: Off Publ Eur Soc Clin Microbiol Infect Dis"},{"key":"4236_CR4","first-page":"S15","volume":"92s","author":"GB Migliori","year":"2020","unstructured":"Migliori GB, Tiberi S, Zumla A, Petersen E, Chakaya JM, Wejse C, Mu\u00f1oz Torrico M, Duarte R, Alffenaar JW, Schaaf HS, Marais BJ, Cirillo DM, Alagna R, Rendon A, Pontali E, Piubello A, Figueroa J, Ferlazzo G, Garc\u00eda-Basteiro A, Centis R, Visca D, D\u2019Ambrosio L, Sotgiu G (2020) MDR\/XDR-TB management of patients and contacts: challenges facing the new decade. The 2020 clinical update by the Global Tuberculosis Network. Int J Infect Dis: IJID : Off Publ Int Soc Infect Dis 92s:S15\u2013s25","journal-title":"Int J Infect Dis: IJID : Off Publ Int Soc Infect Dis"},{"issue":"2","key":"4236_CR5","doi-asserted-by":"publisher","first-page":"326","DOI":"10.1128\/AAC.44.2.326-336.2000","volume":"44","author":"SV Ramaswamy","year":"2000","unstructured":"Ramaswamy SV, Amin AG, G\u00f6ksel S, Stager CE, Dou SJ, El Sahly H, Moghazeh SL, Kreiswirth BN, Musser JM (2000) Molecular genetic analysis of nucleotide polymorphisms associated with ethambutol resistance in human isolates of Mycobacterium tuberculosis. Antimicrob Agents Chemother 44(2):326\u2013336","journal-title":"Antimicrob Agents Chemother"},{"issue":"11","key":"4236_CR6","first-page":"1320","volume":"13","author":"Y Zhang","year":"2009","unstructured":"Zhang Y, Yew WW (2009) Mechanisms of drug resistance in Mycobacterium tuberculosis. Int J Tuberculosis Lung Dis : Off J Int Union Tuberculosis Lung Dis 13(11):1320\u20131330","journal-title":"Int J Tuberculosis Lung Dis : Off J Int Union Tuberculosis Lung Dis"},{"issue":"6","key":"4236_CR7","first-page":"464","volume":"28","author":"Q Li","year":"2015","unstructured":"Li Q, Dong HY, Pang Y, Xia H, Ou XC, Zhang ZY, Li JC, Zhang JK, Huan ST, Chin DP, Kam KM, Zhao YL (2015) Multicenter evaluation of the molecular line probe assay for multidrug resistant mycobacterium tuberculosis detection in China. Biomedical and environmental sciences : BES 28(6):464\u2013467","journal-title":"Biomedical and environmental sciences : BES"},{"issue":"7","key":"4236_CR8","first-page":"e42","volume":"4","author":"JN Torres","year":"2015","unstructured":"Torres JN, Paul LV, Rodwell TC, Victor TC, Amallraja AM, Elghraoui A, Goodmanson AP, Ramirez-Busby SM, Chawla A, Zadorozhny V, Streicher EM, Sirgel FA, Catanzaro D, Rodrigues C, Gler MT, Crudu V, Catanzaro A, Valafar F (2015) Novel katG mutations causing isoniazid resistance in clinical M. tuberculosis isolates. Emerging Microbes Infect 4(7):e42","journal-title":"Emerging Microbes Infect"},{"issue":"1","key":"4236_CR9","first-page":"183","volume":"7","author":"L Liu","year":"2018","unstructured":"Liu L, Jiang F, Chen L, Zhao B, Dong J, Sun L, Zhu Y, Liu B, Zhou Y, Yang J, Zhao Y, Jin Q, Zhang X (2018) The impact of combined gene mutations in inhA and ahpC genes on high levels of isoniazid resistance amongst katG non-315 in multidrug-resistant tuberculosis isolates from China. Emerging Microbes Infect 7(1):183","journal-title":"Emerging Microbes Infect"},{"issue":"5","key":"4236_CR10","doi-asserted-by":"publisher","first-page":"2032","DOI":"10.1128\/AAC.01550-10","volume":"55","author":"PJ Campbell","year":"2011","unstructured":"Campbell PJ, Morlock GP, Sikes RD, Dalton TL, Metchock B, Starks AM, Hooks DP, Cowan LS, Plikaytis BB, Posey JE (2011) Molecular detection of mutations associated with first- and second-line drug resistance compared with conventional drug susceptibility testing of Mycobacterium tuberculosis. Antimicrob Agents Chemother 55(5):2032\u20132041","journal-title":"Antimicrob Agents Chemother"},{"key":"4236_CR11","doi-asserted-by":"crossref","unstructured":"Maningi NE, Daum LT, Rodriguez JD, Said HM, Peters RPH, Sekyere JO, Fischer GW, Chambers JP, Fourie PB (2018) Multi- and extensively drug resistant mycobacterium tuberculosis in South Africa: a molecular analysis of historical isolates. J Clin Microbiol 56(5)","DOI":"10.1128\/JCM.01214-17"},{"issue":"4","key":"4236_CR12","doi-asserted-by":"publisher","first-page":"269","DOI":"10.1016\/j.ijmm.2008.07.001","volume":"299","author":"S Srivastava","year":"2009","unstructured":"Srivastava S, Ayyagari A, Dhole TN, Nyati KK, Dwivedi SK (2009) emb nucleotide polymorphisms and the role of embB306 mutations in Mycobacterium tuberculosis resistance to ethambutol. Int J Med Microbiol : IJMM 299(4):269\u2013280","journal-title":"Int J Med Microbiol : IJMM"},{"issue":"4","key":"4236_CR13","doi-asserted-by":"publisher","first-page":"947","DOI":"10.1093\/jac\/dkt448","volume":"69","author":"R Moure","year":"2014","unstructured":"Moure R, Espa\u00f1ol M, Tud\u00f3 G, Vicente E, Coll P, Gonzalez-Martin J, Mick V, Salvad\u00f3 M, Alcaide F (2014) Characterization of the embB gene in Mycobacterium tuberculosis isolates from Barcelona and rapid detection of main mutations related to ethambutol resistance using a low-density DNA array. J Antimicrob Chemother 69(4):947\u2013954","journal-title":"J Antimicrob Chemother"},{"issue":"8","key":"4236_CR14","doi-asserted-by":"publisher","first-page":"585","DOI":"10.3109\/00365548.2014.918649","volume":"46","author":"J He","year":"2014","unstructured":"He J, Zhu B, Yang Z, Hu B, Lin L, Zhang Q (2014) Molecular analysis of the rpsL gene for rapid detection of streptomycin-resistant Mycobacterium tuberculosis: a meta-analysis. Scand J Infect Dis 46(8):585\u2013592","journal-title":"Scand J Infect Dis"},{"issue":"2","key":"4236_CR15","doi-asserted-by":"publisher","first-page":"596","DOI":"10.1128\/AAC.48.2.596-601.2004","volume":"48","author":"AF Cheng","year":"2004","unstructured":"Cheng AF, Yew WW, Chan EW, Chin ML, Hui MM, Chan RC (2004) Multiplex PCR amplimer conformation analysis for rapid detection of gyrA mutations in fluoroquinolone-resistant Mycobacterium tuberculosis clinical isolates. Antimicrob Agents Chemother 48(2):596\u2013601","journal-title":"Antimicrob Agents Chemother"},{"issue":"5","key":"4236_CR16","doi-asserted-by":"publisher","first-page":"897","DOI":"10.1093\/jac\/dkq047","volume":"65","author":"J Evans","year":"2010","unstructured":"Evans J, Segal H (2010) Novel multiplex allele-specific PCR assays for the detection of resistance to second-line drugs in Mycobacterium tuberculosis. J Antimicrob Chemother 65(5):897\u2013900","journal-title":"J Antimicrob Chemother"},{"issue":"7","key":"4236_CR17","doi-asserted-by":"publisher","first-page":"1417","DOI":"10.1093\/jac\/dkr173","volume":"66","author":"PE Almeida Da Silva","year":"2011","unstructured":"Almeida Da Silva PE, Palomino JC (2011) Molecular basis and mechanisms of drug resistance in Mycobacterium tuberculosis: classical and new drugs. J Antimicrob Chemother 66(7):1417\u20131430","journal-title":"J Antimicrob Chemother"},{"issue":"9","key":"4236_CR18","doi-asserted-by":"publisher","first-page":"2291","DOI":"10.1128\/AAC.44.9.2291-2295.2000","volume":"44","author":"GP Morlock","year":"2000","unstructured":"Morlock GP, Crawford JT, Butler WR, Brim SE, Sikes D, Mazurek GH, Woodley CL, Cooksey RC (2000) Phenotypic characterization of pncA mutants of Mycobacterium tuberculosis. Antimicrob Agents Chemother 44(9):2291\u20132295","journal-title":"Antimicrob Agents Chemother"},{"issue":"Suppl 2","key":"4236_CR19","doi-asserted-by":"publisher","first-page":"S29","DOI":"10.1093\/infdis\/jiu821","volume":"211 Suppl 2","author":"CM Denkinger","year":"2015","unstructured":"Denkinger CM, Kik SV, Cirillo DM, Casenghi M, Shinnick T, Weyer K, Gilpin C, Boehme CC, Schito M, Kimerling M, Pai M (2015) Defining the needs for next generation assays for tuberculosis. J Infect Dis 211 Suppl 2(Suppl 2):S29\u2013S38","journal-title":"J Infect Dis"},{"key":"4236_CR20","doi-asserted-by":"publisher","first-page":"100159","DOI":"10.1016\/j.jctube.2020.100159","volume":"19","author":"NM Mertaniasih","year":"2020","unstructured":"Mertaniasih NM, Soedarsono KT, Koendhori EB, Kusumaningrum D, Koesprijani ST, Nuha Z, Chanifah H (2020) Difficulties with the implemented xpert MTB\/RIF for determining diagnosis of pulmonary and extrapulmonary tuberculosis in adults and children. J Clin Tuberculosis Mycobact Dis 19:100159","journal-title":"J Clin Tuberculosis Mycobact Dis"},{"issue":"1","key":"4236_CR21","doi-asserted-by":"publisher","first-page":"32","DOI":"10.4103\/0974-777X.176143","volume":"8","author":"R Kaur","year":"2016","unstructured":"Kaur R, Kachroo K, Sharma JK, Vatturi SM, Dang A (2016) Diagnostic accuracy of Xpert test in tuberculosis detection: a systematic review and meta-analysis. J Global Infect Dis 8(1):32\u201340","journal-title":"J Global Infect Dis"},{"issue":"7","key":"4236_CR22","doi-asserted-by":"publisher","first-page":"e0180572","DOI":"10.1371\/journal.pone.0180572","volume":"12","author":"AJ Meyer","year":"2017","unstructured":"Meyer AJ, Atuheire C, Worodria W, Kizito S, Katamba A, Sanyu I, Andama A, Ayakaka I, Cattamanchi A, Bwanga F, Huang L, Davis JL (2017) Sputum quality and diagnostic performance of GeneXpert MTB\/RIF among smear-negative adults with presumed tuberculosis in Uganda. PLoS One 12(7):e0180572","journal-title":"PLoS One"},{"key":"4236_CR23","doi-asserted-by":"crossref","unstructured":"Vilch\u00e8ze C, Kremer L (2017) Acid-fast positive and acid-fast negative Mycobacterium tuberculosis: The Koch Paradox. Microbiol Spectr 5(2)","DOI":"10.1128\/microbiolspec.TBTB2-0003-2015"},{"key":"4236_CR24","unstructured":"Y Z, Z L (2013) TB laboratory standardization operation and network establishment. People\u2019s Medical Publishing House, Beijing"},{"issue":"6","key":"4236_CR25","doi-asserted-by":"publisher","first-page":"e100253","DOI":"10.1371\/journal.pone.0100253","volume":"9","author":"W Gong","year":"2014","unstructured":"Gong W, Xiong X, Qi Y, Jiao J, Duan C, Wen B (2014) Identification of novel surface-exposed proteins of Rickettsia rickettsii by affinity purification and proteomics. PLoS One 9(6):e100253","journal-title":"PLoS One"},{"issue":"5","key":"4236_CR26","first-page":"271","volume":"34","author":"L JianQin","year":"2012","unstructured":"JianQin L, HuaFang G, HongMin L, ZhiXian Z, GuangYu Z, JinHe W, SuMin W (2012) Study on the reliability of detecting Mycobacteria in sputum specimens by PCR-fluorescent probe. Chin J Antituberculosis 34(5):271\u2013274","journal-title":"Chin J Antituberculosis"},{"issue":"15","key":"4236_CR27","doi-asserted-by":"publisher","first-page":"10002","DOI":"10.1073\/pnas.142313799","volume":"99","author":"P An","year":"2002","unstructured":"An P, Nelson GW, Wang L, Donfield S, Goedert JJ, Phair J, Vlahov D, Buchbinder S, Farrar WL, Modi W, O\u2019Brien SJ, Winkler CA (2002) Modulating influence on HIV\/AIDS by interacting RANTES gene variants. Proc Natl Acad Sci U S A 99(15):10002\u201310007","journal-title":"Proc Natl Acad Sci U S A"},{"key":"4236_CR28","unstructured":"WHO (2014) WHO Guidelines Approved by the Guidelines Review Committee. Xpert MTB\/RIF implementation manual: technical and operational \u2018how-to\u2019; practical considerations. World Health Organization, Geneva"},{"issue":"11","key":"4236_CR29","doi-asserted-by":"publisher","first-page":"2341","DOI":"10.1093\/jac\/dkq322","volume":"65","author":"G Tud\u00f3","year":"2010","unstructured":"Tud\u00f3 G, Rey E, Borrell S, Alcaide F, Codina G, Coll P, Mart\u00edn-Casabona N, Montemayor M, Moure R, Orcau A, Salvad\u00f3 M, Vicente E, Gonz\u00e1lez-Mart\u00edn J (2010) Characterization of mutations in streptomycin-resistant Mycobacterium tuberculosis clinical isolates in the area of Barcelona. J Antimicrob Chemother 65(11):2341\u20132346","journal-title":"J Antimicrob Chemother"},{"issue":"3","key":"4236_CR30","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1111\/j.1469-0691.2009.02800.x","volume":"16","author":"YJ Sun","year":"2010","unstructured":"Sun YJ, Luo JT, Wong SY, Lee AS (2010) Analysis of rpsL and rrs mutations in Beijing and non-Beijing streptomycin-resistant Mycobacterium tuberculosis isolates from Singapore. Clin Microbiol Infect : Off Publ Eur Soc Clin Microbiol Infect Dis 16(3):287\u2013289","journal-title":"Clin Microbiol Infect : Off Publ Eur Soc Clin Microbiol Infect Dis"},{"issue":"7","key":"4236_CR31","doi-asserted-by":"publisher","first-page":"1585","DOI":"10.1007\/s00204-016-1727-6","volume":"90","author":"L Nguyen","year":"2016","unstructured":"Nguyen L (2016) Antibiotic resistance mechanisms in M. tuberculosis: an update. Arch Toxicol 90(7):1585\u20131604","journal-title":"Arch Toxicol"},{"issue":"1","key":"4236_CR32","doi-asserted-by":"publisher","first-page":"2600","DOI":"10.1038\/s41598-020-59558-3","volume":"10","author":"J Perdig\u00e3o","year":"2020","unstructured":"Perdig\u00e3o J, Gomes P, Miranda A, Maltez F, Machado D, Silva C, Phelan JE, Brum L, Campino S, Couto I, Viveiros M, Clark TG, Portugal I (2020) Using genomics to understand the origin and dispersion of multidrug and extensively drug resistant tuberculosis in Portugal. Sci Rep 10(1):2600","journal-title":"Sci Rep"},{"issue":"5","key":"4236_CR33","doi-asserted-by":"publisher","first-page":"621","DOI":"10.1164\/rccm.201510-2091OC","volume":"194","author":"MR Farhat","year":"2016","unstructured":"Farhat MR, Sultana R, Iartchouk O, Bozeman S, Galagan J, Sisk P, Stolte C, Nebenzahl-Guimaraes H, Jacobson K, Sloutsky A, Kaur D, Posey J, Kreiswirth BN, Kurepina N, Rigouts L, Streicher EM, Victor TC, Warren RM, van Soolingen D, Murray M (2016) Genetic determinants of drug resistance in Mycobacterium tuberculosis and their diagnostic value. Am J Respir Crit Care Med 194(5):621\u2013630","journal-title":"Am J Respir Crit Care Med"},{"issue":"3","key":"4236_CR34","doi-asserted-by":"publisher","first-page":"727","DOI":"10.1128\/JCM.02775-15","volume":"54","author":"MR Farhat","year":"2016","unstructured":"Farhat MR, Jacobson KR, Franke MF, Kaur D, Sloutsky A, Mitnick CD, Murray M (2016) Gyrase mutations are associated with variable levels of fluoroquinolone resistance in Mycobacterium tuberculosis. J Clin Microbiol 54(3):727\u2013733","journal-title":"J Clin Microbiol"}],"container-title":["European Journal of Clinical Microbiology &amp; Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10096-021-04236-z.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10096-021-04236-z\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10096-021-04236-z.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,8,6]],"date-time":"2021-08-06T09:21:38Z","timestamp":1628241698000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10096-021-04236-z"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4,1]]},"references-count":34,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2021,9]]}},"alternative-id":["4236"],"URL":"https:\/\/doi.org\/10.1007\/s10096-021-04236-z","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-67817\/v1","asserted-by":"object"}]},"ISSN":["0934-9723","1435-4373"],"issn-type":[{"value":"0934-9723","type":"print"},{"value":"1435-4373","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4,1]]},"assertion":[{"value":"5 December 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 March 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 April 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The study protocol was approved by the Research Ethics Committee of the 8th Medical Center of Chinese PLA General Hospital. Written informed consent was obtained from all adult participants.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval and consent to participate"}},{"value":"The study protocol was approved by the Research Ethics Committee of the 8th Medical Center of Chinese PLA General Hospital, and the informed consent was obtained from all adult participants and parents of the participant under 16 years old.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to participate"}},{"value":"Not available.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}