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The aim of this study was (1) to investigate the relationship of specific topoisomerase mutations on the stability of IncF (CTX-M producing) plasmids using isogenic <jats:italic>E. coli<\/jats:italic> mutants and (2) to investigate the impact of the IncF-type plasmids present in the <jats:italic>E. coli<\/jats:italic> clone ST131 on the evolution of quinolone resistance. <jats:italic>E. coli<\/jats:italic> ATCC 25922 (background strain) and derived mutants encoding specific QRDR substitutions were used. Also, NGS-characterized IncFIA and IncFIB plasmids (encoding CTX-M genes) were included. Plasmid stability was evaluated by sequential dilutions into Luria broth medium without antibiotics for 7\u00a0days. Mutant frequency to ciprofloxacin was also evaluated. Moderate differences in the IncF plasmids stability were observed among <jats:italic>E. coli<\/jats:italic> ATCC 25922 and isogenic mutants. Under our experimental conditions, the fluctuation of bacteria harboring plasmids was less than 0.5-log<jats:sub>(10)<\/jats:sub> in all cases. In the mutant frequency tests, it was observed that the presence of these IncF plasmids increased this value significantly (10\u20131000-fold). Quinolone resistance substitutions in <jats:italic>gyrA<\/jats:italic> or <jats:italic>parC<\/jats:italic> genes, frequently found associated with <jats:italic>E. coli<\/jats:italic> clone ST131, do not modify the stability of ST131-associated IncFIA and IncFIB plasmids under <jats:italic>in vitro<\/jats:italic> conditions. IncF-type plasmids present in <jats:italic>E. coli<\/jats:italic> clone ST131 facilitate the selection of resistance to quinolones. These results are consistent with the clinical scenario in which the combination of resistance to quinolones and beta-lactams is highly frequent in the <jats:italic>E. coli<\/jats:italic> clone ST131.<\/jats:p>","DOI":"10.1007\/s10096-021-04358-4","type":"journal-article","created":{"date-parts":[[2021,11,17]],"date-time":"2021-11-17T12:09:48Z","timestamp":1637150988000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Interplay between IncF plasmids and topoisomerase mutations conferring quinolone resistance in the Escherichia coli ST131 clone: stability and resistance evolution"],"prefix":"10.1007","author":[{"given":"Jose-Manuel","family":"Rodr\u00edguez-Mart\u00ednez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorena","family":"Lopez-Cerero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Garc\u00eda-Duque","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jesus","family":"Rodriguez-Ba\u00f1o","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alvaro","family":"Pascual","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,11,17]]},"reference":[{"key":"4358_CR1","doi-asserted-by":"publisher","unstructured":"Pitout JDD (2012) Extraintestinal pathogenic Escherichia coli: an update on antimicrobial resistance, laboratory diagnosis and treatment. 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