{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T23:36:53Z","timestamp":1791243413036,"version":"4.1.0"},"reference-count":24,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2022,2,25]],"date-time":"2022-02-25T00:00:00Z","timestamp":1645747200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2022,2,25]],"date-time":"2022-02-25T00:00:00Z","timestamp":1645747200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Eur J Clin Microbiol Infect Dis"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract\n<\/jats:title><jats:p>With the increase in antimicrobial resistance, fast reporting of antimicrobial susceptibility testing (AST) results is becoming increasingly important. EUCAST developed a method for rapid AST (RAST) directly from the broth of positive blood cultures (BC). Inhibition zones are read after 4, 6, and 8\u00a0h, with specific breakpoints per time point. We evaluated the RAST method based on EUCAST disk diffusion methodology with inoculation of BC broth using WASPLab<jats:sup>\u00ae<\/jats:sup> (inclusive Colibr\u00ed<jats:sup>\u2122<\/jats:sup> and Radian<jats:sup>\u00ae<\/jats:sup>). Forty-nine non-duplicate strains were tested: <jats:italic>Escherichia coli n<\/jats:italic>\u2009=\u200917, <jats:italic>Klebsiella pneumoniae n<\/jats:italic>\u2009=\u20097, <jats:italic>Pseudomonas aeruginosa n<\/jats:italic>\u2009=\u20094, <jats:italic>Acinetobacter baumannii n<\/jats:italic>\u2009=\u20092, <jats:italic>Staphylococcus aureus n<\/jats:italic>\u2009=\u200910, <jats:italic>Enterococcus faecalis n<\/jats:italic>\u2009=\u20096, and <jats:italic>Enterococcus faecium n<\/jats:italic>\u2009=\u20093. Results were compared to direct AST and standardized AST. Good categorical agreement was obtained at all time points for all groups, except <jats:italic>P. aeruginosa<\/jats:italic>. RAST cut-offs for extended-spectrum \u03b2-lactamase (ESBL)-producing <jats:italic>Enterobacterales<\/jats:italic> enabled the detection of all included ESBL isolates (<jats:italic>n<\/jats:italic>\u2009=\u20095) at all time points, except for 1 <jats:italic>E. coli<\/jats:italic> ESBL after 4\u00a0h. RAST cut-offs for carbapenemase-producing <jats:italic>Enterobacterales<\/jats:italic> enabled the detection of only one carbapenemase after 6\u00a0h. However, all carbapenemases (<jats:italic>n<\/jats:italic>\u2009=\u20093) were correctly detected after 8\u00a0h. Two methicillin-resistant <jats:italic>S. aureus<\/jats:italic> were included; both were correctly categorized as cefoxitin-resistant at 6 and 8\u00a0h. At 4\u00a0h, there was insufficient growth for inhibition zone interpretation. EUCAST RAST is a fast supplementary tool for direct AST of positive BC. WASPLab<jats:sup>\u00ae<\/jats:sup> provides a significant advantage as pictures are made automatically implicating that we are not strictly bound to the time points for inhibition zone interpretation.<\/jats:p>","DOI":"10.1007\/s10096-022-04421-8","type":"journal-article","created":{"date-parts":[[2022,2,25]],"date-time":"2022-02-25T22:02:33Z","timestamp":1645826553000},"page":"733-739","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Validation of Rapid Antimicrobial Susceptibility Testing directly from blood cultures using WASPLab\u00ae, including Colibr\u00ed\u2122 and Radian\u00ae in-Line Carousel"],"prefix":"10.1007","volume":"41","author":[{"given":"Pauline Hilda","family":"Herroelen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robbe","family":"Heestermans","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristof","family":"Emmerechts","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristof","family":"Vandoorslaer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid","family":"Wybo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denis","family":"Pi\u00e9rard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2655-4322","authenticated-orcid":false,"given":"Astrid","family":"Muyldermans","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,2,25]]},"reference":[{"issue":"10219","key":"4421_CR1","doi-asserted-by":"publisher","first-page":"200","DOI":"10.1016\/S0140-6736(19)32989-7","volume":"395","author":"KE Rudd","year":"2020","unstructured":"Rudd KE et al (2020) Global, regional, and national sepsis incidence and mortality, 1990\u20132017: analysis for the Global Burden of Disease Study. 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