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This study aimed to characterize a hypervirulent tigecycline-resistant and carbapenem-resistant <jats:italic>Klebsiella pneumoniae<\/jats:italic> strain, XJ-K2, collected from a patient\u2019s blood. We tested antimicrobial susceptibility, virulence, and whole-genome sequencing (WGS) on strain XJ-K2. WGS data were used to identify virulence and resistance genes and to perform multilocus sequence typing (MLST) and phylogenetic analysis. Three novel plasmids, including a pLVPK-like virulence plasmid (pXJ-K2-p1) and two multiple resistance plasmids (pXJ-K2-KPC-2 and pXJ-K2-p3), were discovered in strain XJ-K2. The IncFII(pCRY) plasmid pXJ-K2-p3 carried the <jats:italic>dfrA14<\/jats:italic>, <jats:italic>sul2<\/jats:italic>, <jats:italic>qnrS1<\/jats:italic>, <jats:italic>bla<\/jats:italic><jats:sub>LAP-2<\/jats:sub>, and <jats:italic>tet<\/jats:italic>(A) resistance genes. The IncFII(pHN7A8)\/IncR plasmid pXJ-K2-KPC-2 also carried a range of resistance elements, containing <jats:italic>rmtB<\/jats:italic>, <jats:italic>bla<\/jats:italic><jats:sub>KPC-2<\/jats:sub>, <jats:italic>bla<\/jats:italic><jats:sub>TEM-1<\/jats:sub>, <jats:italic>bla<\/jats:italic><jats:sub>CTX-M-65<\/jats:sub>, and <jats:italic>fosA3<\/jats:italic>. MLST analysis revealed that strain XJ-K2 belonged to sequence type 11 (ST11). Seven complete phage sequences and many virulence genes were found in strain XJ-K2. Meanwhile, antimicrobial susceptibility tests and <jats:italic>G. mellonella<\/jats:italic> larval infection models confirmed the extensively drug resistance (XDR) and hypervirulence of KJ-K2. To our knowledge, this is the first observation and description of the ST11 hypervirulent tigecycline- and carbapenem-resistant <jats:italic>K. pneumoniae<\/jats:italic> strain co-carrying <jats:italic>bla<\/jats:italic><jats:sub>KPC-2<\/jats:sub> and the <jats:italic>tet<\/jats:italic>(A) in a patient\u2019s blood in China. Further investigation is needed to understand the resistance and virulence mechanisms of this significant hypervirulent tigecycline- and carbapenem-resistant strain.<\/jats:p>","DOI":"10.1007\/s10096-022-04512-6","type":"journal-article","created":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T07:02:31Z","timestamp":1667372551000},"page":"23-31","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Coexistence of tet(A) and blaKPC-2 in the ST11 hypervirulent tigecycline- and carbapenem-resistant Klebsiella pneumoniae isolated from a blood sample"],"prefix":"10.1007","volume":"42","author":[{"given":"Xiaokui","family":"Zhu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changwu","family":"Yue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huaixin","family":"Geng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingjie","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiming","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianqin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuanyu","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangxin","family":"Luan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1612-1136","authenticated-orcid":false,"given":"Xu","family":"Jia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,11,2]]},"reference":[{"key":"4512_CR1","doi-asserted-by":"publisher","first-page":"252","DOI":"10.1093\/femsre\/fux013","volume":"41","author":"S Navon-Venezia","year":"2017","unstructured":"Navon-Venezia S, Kondratyeva K, Carattoli A (2017) Klebsiella pneumoniae: a major worldwide source and shuttle for antibiotic resistance. 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