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The objective of this study was to use the cobas<jats:sup>\u00ae<\/jats:sup> TV\/MG assay to assess the point prevalence of TV and MG in specimens from men and women over a broad European geographical area. Urine, vaginal, endocervical, and rectal samples were collected from patients aged\u2009\u2265\u200918\u00a0years receiving <jats:italic>Chlamydia trachomatis<\/jats:italic> (CT) and\/or <jats:italic>Neisseria gonorrhoeae<\/jats:italic> (NG) screening as per local standard of care at sites in Belgium, Germany, Spain, and the UK (Wales). Remnant samples were assessed using the cobas TV\/MG assay. Analysis of 2795 samples showed that MG prevalence varied slightly across female sample types (range: 1.7\u20135.8%; <jats:italic>p<\/jats:italic>\u2009=\u20090.0042). MG prevalence was higher in male rectal samples (12.5%) than in male urine samples (3.9%; <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001). TV prevalence was low in male (0.8%; 12\/1535) and female (1.3%; 16\/1260) samples across all sites. Co-infection of TV\/MG with CT or NG was 10.0% (19\/190) and 9.6% (7\/73), respectively, in both male and female samples. MG and TV prevalence rates were comparable to the published literature in Europe. MG prevalence was highest in male rectal samples; as rectal testing is an off-label use of the cobas TV\/MG assay, the clinical utility of this assay for rectal testing should be further investigated.<\/jats:p>","DOI":"10.1007\/s10096-022-04521-5","type":"journal-article","created":{"date-parts":[[2022,11,11]],"date-time":"2022-11-11T21:02:49Z","timestamp":1668200569000},"page":"43-52","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["The prevalence of Mycoplasma genitalium (MG) and Trichomonas vaginalis (TV) at testing centers in Belgium, Germany, Spain, and the UK using the cobas TV\/MG molecular assay"],"prefix":"10.1007","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7535-5899","authenticated-orcid":false,"given":"Michael D.","family":"Perry","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7915-8921","authenticated-orcid":false,"given":"Sophie","family":"Jones","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9401-0071","authenticated-orcid":false,"given":"Alexander","family":"Bertram","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4318-858X","authenticated-orcid":false,"given":"Adolfo","family":"de Salazar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2236-0958","authenticated-orcid":false,"given":"Antonio","family":"Barrientos-Dur\u00e1n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1874-8933","authenticated-orcid":false,"given":"Gilberte","family":"Schiettekatte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Lewinski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodney","family":"Arcenas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Avneet","family":"Hansra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Merlin","family":"Njoya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7611-781X","authenticated-orcid":false,"given":"Federico","family":"Garc\u00eda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,11,11]]},"reference":[{"key":"4521_CR1","doi-asserted-by":"publisher","first-page":"458","DOI":"10.1097\/olq.0000000000001002","volume":"46","author":"A Lockhart","year":"2019","unstructured":"Lockhart A, Senkomago V, Ting J, Chitwa M, Kimani J, Gakure H et al (2019) Prevalence and risk factors of Trichomonas vaginalis among female sexual workers in Nairobi, Kenya. 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Accessed 25 October 2022."}],"container-title":["European Journal of Clinical Microbiology &amp; Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10096-022-04521-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10096-022-04521-5\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10096-022-04521-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,9]],"date-time":"2023-01-09T03:05:11Z","timestamp":1673233511000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10096-022-04521-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,11]]},"references-count":41,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,1]]}},"alternative-id":["4521"],"URL":"https:\/\/doi.org\/10.1007\/s10096-022-04521-5","relation":{},"ISSN":["0934-9723","1435-4373"],"issn-type":[{"value":"0934-9723","type":"print"},{"value":"1435-4373","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,11]]},"assertion":[{"value":"4 July 2022","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 November 2022","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 November 2022","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The study was conducted in compliance with International Conference on Harmonisation (ICH) guidelines and Good Clinical Practice, and the protocol was approved by the Institutional Review Board (IRB) codes: 1559-N-18, UK NHS Health Research Authority Research Ethics Committee Reference Number \u2013 18\/EE\/0334, 0175-N-19, CEIM\/CEI Provincial de Granada, Comunidad Aut\u00f3noma de Andaluc\u00eda, Spain, EC UZA 18\/47\/545, Medical Association of Lower Saxony approval number Bo\/32\/2018.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"For AME, CMA, and PHW, no informed consent was deemed necessary according to the ethical committee due to use of remnant and de-identified samples for the study. For USC, verbal informed consent was obtained.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to participate"}},{"value":"Not required.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"ML, RA, AH, and MN are employees of Roche Molecular Systems. ML, RA, and AH report receiving stocks and stock options from Roche. MDP reports receiving funding from Roche for this study and an investigator-initiated study, as well as honoraria and funding to speak at and attend past meetings from Roche. FG reports receiving honoraria, grant, and advisory board support from Gilead Sciences, ViiV Healthcare, Janssen, Merck Sharp & Dohme, Theratechnologies, AbbVie, Werfen, Roche, Accelerate, and Menarini. GS reports receiving reagents from Roche to perform the testing during the study. AB reports receiving funding from Roche directly to their institution for sample analysis during the study. SJ reports receiving funding from Roche directly to their institution for this study. AdS and ABD have nothing to disclose.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}