{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T14:31:17Z","timestamp":1793197877115,"version":"4.5.0"},"reference-count":10,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2023,3,16]],"date-time":"2023-03-16T00:00:00Z","timestamp":1678924800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2023,3,16]],"date-time":"2023-03-16T00:00:00Z","timestamp":1678924800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Eur J Clin Microbiol Infect Dis"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>PCR detection of <jats:italic>Helicobacter pylori<\/jats:italic> infection in gastric biopsies allows the detection of this bacterium and the mutations associated with macrolide resistance. The aim of this study was to evaluate the performance of RIDA\u00aeGENE <jats:italic>H. pylori<\/jats:italic> PCR (r-Biopharm) on the ELITe InGenius System (Elitech). Two hundred gastric biopsies were obtained. These biopsies were ground in nutrient broth. Two hundred microliters of this suspension was treated with proteinase K, and then, 200 \u00b5L was transferred to an ELITe InGenius sample tube and tested using RIDA\u00aeGENE <jats:italic>H. pylori<\/jats:italic> PCR reagents. In-house <jats:italic>H. pylori<\/jats:italic> PCR was used as a reference. The sensitivity of RIDA\u00aeGENE <jats:italic>H. pylori<\/jats:italic> PCR with ELITe InGenius was 100%, the specificity was 98% (95% confidence interval (CI), 95.3\u2013100%), the PPV was 98% (95% CI, 95.3\u2013100%), and the NPV was 100% for the detection of <jats:italic>H. pylori<\/jats:italic>. All of these parameters were 100% for the categorization of macrolide resistance. The adaptation of RIDA\u00aeGENE <jats:italic>H. pylori<\/jats:italic> PCR reagents on the ELITe InGenius System was successful. This PCR is easy to use on this system.<\/jats:p>","DOI":"10.1007\/s10096-023-04563-3","type":"journal-article","created":{"date-parts":[[2023,3,26]],"date-time":"2023-03-26T23:46:40Z","timestamp":1679874400000},"page":"593-596","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["RIDA\u00aeGENE Helicobacter pylori PCR on the ELITe InGenius System"],"prefix":"10.1007","volume":"42","author":[{"given":"Lucie","family":"B\u00e9n\u00e9jat","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Astrid","family":"Ducournau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chlo\u00e9 Domingues","family":"Martins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilie","family":"Bess\u00e8de","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6077-1825","authenticated-orcid":false,"given":"Philippe","family":"Lehours","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,3,16]]},"reference":[{"key":"4563_CR1","doi-asserted-by":"crossref","unstructured":"Megraud F, Lehours P. Helicobacter pylori detection and antimicrobial susceptibility testing. Clin Microbiol Rev [Internet] 2007;20:280\u2013322. http:\/\/www.ncbi.nlm.nih.gov\/entrez\/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17428887","DOI":"10.1128\/CMR.00033-06"},{"key":"4563_CR2","doi-asserted-by":"publisher","first-page":"12512","DOI":"10.1111\/hel.12512","volume":"23","author":"L B\u00e9n\u00e9jat","year":"2018","unstructured":"B\u00e9n\u00e9jat L, Ducournau A, Lehours P, M\u00e9graud F (2018) Real-time PCR for Helicobacter pylori diagnosis The best tools available. Helicobacter 23:12512. https:\/\/doi.org\/10.1111\/hel.12512","journal-title":"Helicobacter"},{"key":"4563_CR3","doi-asserted-by":"publisher","first-page":"12702","DOI":"10.1111\/hel.12702","volume":"25","author":"Q Jehanne","year":"2020","unstructured":"Jehanne Q, B\u00e9n\u00e9jat L, M\u00e9graud F, Bess\u00e8de E, Lehours P (2020) Evaluation of the AllplexTM H pylori and ClariR PCR Assay for Helicobacter pylori detection on gastric biopsies. Helicobacter 25:12702. https:\/\/doi.org\/10.1111\/hel.12702","journal-title":"Helicobacter"},{"key":"4563_CR4","doi-asserted-by":"publisher","first-page":"12560","DOI":"10.1111\/hel.12560","volume":"24","author":"C Hays","year":"2019","unstructured":"Hays C, Delerue T, Lamarque D, Burucoa C, Collobert G, Bill\u00f6et A et al (2019) Molecular diagnosis of Helicobacter pylori infection in gastric biopsies: Evaluation of the Amplidiag\u00ae H pylori + ClariR assay. Helicobacter 24:12560. https:\/\/doi.org\/10.1111\/hel.12560","journal-title":"Helicobacter"},{"key":"4563_CR5","doi-asserted-by":"publisher","unstructured":"Berenger BM, Chui L, Ferrato C, Lloyd T, Li V, Pillai DR. Performance of four commercial real-time PCR assays for the detection of bacterial enteric pathogens in clinical samples. Int. J. Infect. Dis. IJID Off. Publ. Int. Soc. Infect. Dis. 2021 23;S1201-9712(21)00831-6. https:\/\/doi.org\/10.1016\/j.ijid.2021.10.035","DOI":"10.1016\/j.ijid.2021.10.035"},{"key":"4563_CR6","doi-asserted-by":"publisher","first-page":"875","DOI":"10.1007\/s10096-022-04444-1","volume":"41","author":"L B\u00e9n\u00e9jat","year":"2022","unstructured":"B\u00e9n\u00e9jat L, Giese A, Lescaudron Z, Bonnac J, Ducournau A, Bess\u00e8de E et al (2022) Automation of RIDA\u00aeGENE Helicobacter pylori PCR on the BD MAXTM System. Eur. J. Clin. Microbiol. Infect. Dis. Off. Publ. Eur. Soc. Clin. Microbiol. 41:875\u20139. https:\/\/doi.org\/10.1007\/s10096-022-04444-1","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis. Off. Publ. Eur. Soc. Clin. Microbiol."},{"key":"4563_CR7","doi-asserted-by":"publisher","first-page":"12855","DOI":"10.1111\/hel.12855","volume":"26","author":"L B\u00e9n\u00e9jat","year":"2021","unstructured":"B\u00e9n\u00e9jat L, Ducournau A, Domingues-Martins C, Lecoeur M, Blosse A, M\u00e9graud F et al (2021) Adaptation of an in-house PCR for the detection of Helicobacter pylori and the mutations associated with macrolide resistance into ready-to-use PCR microwell strips. Helicobacter 26:12855. https:\/\/doi.org\/10.1111\/hel.12855","journal-title":"Helicobacter"},{"key":"4563_CR8","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1007\/978-1-0716-1302-3_6","volume":"2283","author":"P Lehours","year":"2021","unstructured":"Lehours P, M\u00e9graud F (2021) Culture-Based Antimicrobial Susceptibility Testing for Helicobacter pylori. Methods Mol. Biol. Clifton NJ 2283:45\u201350. https:\/\/doi.org\/10.1007\/978-1-0716-1302-3_6","journal-title":"Methods Mol. Biol. Clifton NJ"},{"key":"4563_CR9","doi-asserted-by":"publisher","first-page":"8","DOI":"10.3390\/jcm8010053","volume":"7","author":"FN Lauener","year":"2019","unstructured":"Lauener FN, Imkamp F, Lehours P, Buissonni\u00e8re A, Benejat L, Zbinden R et al (2019) Genetic Determinants and Prediction of Antibiotic Resistance Phenotypes in Helicobacter pylori. J. Clin. Med. 7:8. https:\/\/doi.org\/10.3390\/jcm8010053","journal-title":"J. Clin. Med."},{"key":"4563_CR10","doi-asserted-by":"publisher","first-page":"715","DOI":"10.1016\/j.cmi.2016.06.003","volume":"22","author":"A Ducournau","year":"2016","unstructured":"Ducournau A, B\u00e9n\u00e9jat L, Sifr\u00e9 E, Bess\u00e8de E, Lehours P, M\u00e9graud F (2016) Helicobacter pylori resistance to antibiotics in 2014 in France detected by phenotypic and genotypic methods. Clin. Microbiol. Infect. Off. Publ. Eur. Soc. Clin. 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Dis."}],"container-title":["European Journal of Clinical Microbiology &amp; Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10096-023-04563-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10096-023-04563-3\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10096-023-04563-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,15]],"date-time":"2023-04-15T08:14:02Z","timestamp":1681546442000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10096-023-04563-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,16]]},"references-count":10,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2023,5]]}},"alternative-id":["4563"],"URL":"https:\/\/doi.org\/10.1007\/s10096-023-04563-3","relation":{},"ISSN":["0934-9723","1435-4373"],"issn-type":[{"value":"0934-9723","type":"print"},{"value":"1435-4373","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,16]]},"assertion":[{"value":"16 December 2022","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 February 2023","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 March 2023","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All diagnostic methods were performed retrospectively. All patients were investigated in a hospital and private clinical setting according to good clinical practices. In this routine process, consent for the endoscopic procedure is always provided in writing and maintained in the patient\u2019s medical record.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"No informed consent for using human gastric DNA was requested from the patients.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to participate"}},{"value":"Not applicable","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}