{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T15:59:12Z","timestamp":1767974352794,"version":"3.49.0"},"reference-count":35,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2023,2,22]],"date-time":"2023-02-22T00:00:00Z","timestamp":1677024000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2023,2,22]],"date-time":"2023-02-22T00:00:00Z","timestamp":1677024000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100012957","name":"Friedrich-Schiller-Universit\u00e4t Jena","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100012957","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Eur J Clin Microbiol Infect Dis"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Identification of <jats:italic>Salmonella<\/jats:italic> serovars is performed by conventional seroagglutination or sequencing. These methods are labor-intensive and require technical experience. An easy-to-perform assay allowing the timely identification of the most common non-typhoidal serovars (NTS) is needed. In this study, a molecular assay based on loop-mediated isothermal amplification (LAMP) targeting specific gene sequences of <jats:italic>Salmonella<\/jats:italic> Enteritidis, <jats:italic>S.<\/jats:italic> Typhimurium, <jats:italic>S.<\/jats:italic> Infantis, <jats:italic>S.<\/jats:italic> Derby, and <jats:italic>S.<\/jats:italic> Choleraesuis has been developed for rapid serovar identification from cultured colonies. A total of 318 <jats:italic>Salmonella<\/jats:italic> strains and 25 isolates of other <jats:italic>Enterobacterales<\/jats:italic> species that served as negative controls were analyzed. All <jats:italic>S.<\/jats:italic> Enteritidis (<jats:italic>n<\/jats:italic>\u2009=\u200940), <jats:italic>S.<\/jats:italic> Infantis (<jats:italic>n<\/jats:italic>\u2009=\u200927), and <jats:italic>S.<\/jats:italic> Choleraesuis (<jats:italic>n<\/jats:italic>\u2009=\u200911) strains were correctly identified. Seven out of 104 <jats:italic>S.<\/jats:italic> Typhimurium and 10 out of 38 <jats:italic>S.<\/jats:italic> Derby strains missed a positive signal. Cross-reactions of the gene targets were only rarely observed and restricted to the <jats:italic>S.<\/jats:italic> Typhimurium primer set (5 false-positives). Sensitivity and specificity of the assay compared to seroagglutination were as follows: 100% and 100% for <jats:italic>S.<\/jats:italic> Enteritidis, 93.3% and 97.7% for <jats:italic>S.<\/jats:italic> Typhimurium, 100% and 100% for <jats:italic>S.<\/jats:italic> Infantis, 73.7% and 100% for<jats:italic> S<\/jats:italic>. Derby, and 100% and 100% for <jats:italic>S.<\/jats:italic> Choleraesuis, respectively. With results available in just a few minutes of hands-on time and a test run time of 20\u00a0min, the LAMP assay developed here may be a useful tool for the rapid identification of common <jats:italic>Salmonella<\/jats:italic> NTS in daily routine diagnostics.<\/jats:p>","DOI":"10.1007\/s10096-023-04571-3","type":"journal-article","created":{"date-parts":[[2023,2,22]],"date-time":"2023-02-22T01:02:35Z","timestamp":1677027755000},"page":"461-470","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Development of a rapid diagnostic test based on loop-mediated isothermal amplification to identify the most frequent non-typhoidal Salmonella serovars from culture"],"prefix":"10.1007","volume":"42","author":[{"given":"Birgit","family":"Edel","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Gl\u00f6ckner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sylvia","family":"Stoll","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nora","family":"Lindig","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katharina","family":"Boden","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lars","family":"Wassill","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"Simon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bettina","family":"L\u00f6ffler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3893-0679","authenticated-orcid":false,"given":"J\u00fcrgen","family":"R\u00f6del","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2023,2,22]]},"reference":[{"issue":"14","key":"4571_CR1","doi-asserted-by":"publisher","first-page":"e00591","DOI":"10.1128\/AEM.00591-19","volume":"85","author":"RG Ferrari","year":"2019","unstructured":"Ferrari RG, Rosario DKA, Cunha-Neto A, Mano SB, Figueiredo EES, Conte-Junior CA (2019) Worldwide epidemiology of Salmonella serovars in animal-based foods: a meta-analysis. Appl Environ Microbiol 85(14):e00591-e619. https:\/\/doi.org\/10.1128\/AEM.00591-19","journal-title":"Appl Environ Microbiol"},{"key":"4571_CR2","doi-asserted-by":"publisher","first-page":"19","DOI":"10.3390\/pathogens8010019","volume":"8","author":"J Campos","year":"2019","unstructured":"Campos J, Mour\u00e3o J, Peixe L, Antunes P (2019) Non-typhoidal Salmonella in the pig production chain: a comprehensive analysis of its impact on human health. Pathogens 8:19. https:\/\/doi.org\/10.3390\/pathogens8010019","journal-title":"Pathogens"},{"key":"4571_CR3","doi-asserted-by":"publisher","first-page":"110","DOI":"10.1016\/j.cmi.2015.12.004","volume":"22","author":"P Antunes","year":"2016","unstructured":"Antunes P, Mour\u00e3o J, Campos J, Peixe L (2016) Salmonellosis: the role of poultry meat. Clin Microbiol Infect 22:110\u2013121. https:\/\/doi.org\/10.1016\/j.cmi.2015.12.004","journal-title":"Clin Microbiol Infect"},{"key":"4571_CR4","unstructured":"Grimont ADG, Weill FX (2007) Antigenic formulae of the Salmonella serovars, 9th edn. WHO Collaborating Center for Reference and Research on Salmonella, Institut Pasteur. https:\/\/www.pasteur.fr\/sites\/default\/files\/veng_0.pdf. Accessed 29 Oct\u00a02019"},{"key":"4571_CR5","doi-asserted-by":"publisher","first-page":"4333","DOI":"10.1038\/s41598-020-61254-1","volume":"10","author":"S Banerji","year":"2020","unstructured":"Banerji S, Simon S, Tille A, Fruth A, Flieger A (2020) Genome-based Salmonella serotyping as the new gold standard. Sci Rep 10:4333. https:\/\/doi.org\/10.1038\/s41598-020-61254-1","journal-title":"Sci Rep"},{"key":"4571_CR6","doi-asserted-by":"publisher","unstructured":"Langridge GC, Wain J, Nair S (2012) Invasive salmonellosis in humans. EcoSal Plus 5(1). https:\/\/doi.org\/10.1128\/ecosalplus.8.6.2.2","DOI":"10.1128\/ecosalplus.8.6.2.2"},{"issue":"Suppl 2","key":"4571_CR7","doi-asserted-by":"publisher","first-page":"S127","DOI":"10.1093\/cid\/ciaa315","volume":"71","author":"S Kariuki","year":"2020","unstructured":"Kariuki S, Owusu-Dabo E (2020) Research on invasive nontyphoidal Salmonella disease and developments towards better understanding of epidemiology, management, and control strategies. Clin Infect Dis 71(Suppl 2):S127\u2013S129. https:\/\/doi.org\/10.1093\/cid\/ciaa315","journal-title":"Clin Infect Dis"},{"key":"4571_CR8","doi-asserted-by":"publisher","first-page":"e0008121","DOI":"10.1371\/journal.pntd.0008121","volume":"14","author":"B Tack","year":"2020","unstructured":"Tack B, Phoba MF, Barb\u00e9 B, Kalonji LM, Hardy L, Van Puyvelde S, Ingelbeen B, Falay D, Ngonda D, van der Sande MAB, Deborggraeve S, Jacobs J, Lunguya O (2020) Non-typhoidal Salmonella bloodstream infections in Kisantu, DR Congo: emergence of O5-negative Salmonella Typhimurium and extensive drug resistance. PLoS Negl Trop Dis 14:e0008121. https:\/\/doi.org\/10.1371\/journal.pntd.0008121","journal-title":"PLoS Negl Trop Dis"},{"key":"4571_CR9","doi-asserted-by":"publisher","unstructured":"EFSA and ECDC (European Food Safety Authority and European Centre for Disease Prevention and Control) (2021) The European Union One Health 2020 Zoonoses Report. EFSA J 19:6971, 324 pp. https:\/\/doi.org\/10.2903\/j.efsa.2021.6971","DOI":"10.2903\/j.efsa.2021.6971"},{"key":"4571_CR10","doi-asserted-by":"publisher","first-page":"708","DOI":"10.1016\/j.jmoldx.2020.02.006","volume":"22","author":"X Zhang","year":"2020","unstructured":"Zhang X, Payne M, Wang Q, Sintchenko V, Lan R (2020) Highly sensitive and specific detection and serotyping of five prevalent Salmonella serovars by multiple cross-displacement amplification. J Mol Diagn 22:708\u2013719. https:\/\/doi.org\/10.1016\/j.jmoldx.2020.02.006","journal-title":"J Mol Diagn"},{"issue":"1","key":"4571_CR11","doi-asserted-by":"publisher","first-page":"67","DOI":"10.3390\/microorganisms9010067","volume":"9","author":"SM Yang","year":"2021","unstructured":"Yang SM, Baek J, Kim E, Kim HB, Ko S, Kim D, Yoon H, Kim HY (2021) Development of a genoserotyping method for Salmonella Infantis detection on the basis of pangenome analysis. Microorganisms 9(1):67. https:\/\/doi.org\/10.3390\/microorganisms9010067","journal-title":"Microorganisms"},{"key":"4571_CR12","doi-asserted-by":"publisher","first-page":"223","DOI":"10.12688\/wellcomeopenres.16291.2","volume":"5","author":"M Achtman","year":"2021","unstructured":"Achtman M, Zhou Z, Alikhan NF, Tyne W, Parkhill J, Cormican M, Chiou CS, Torpdahl M, Litrup E, Prendergast DM, Moore JE, Strain S, Kornschober C, Meinersmann R, Uesbeck A, Weill FX, Coffey A, Andrews-Polymenis H, Curtiss RdR, Fanning S (2021) Wellcome Open Res 5:223. https:\/\/doi.org\/10.12688\/wellcomeopenres.16291.2","journal-title":"Wellcome Open Res"},{"key":"4571_CR13","doi-asserted-by":"publisher","first-page":"e00038","DOI":"10.1128\/JCM.00038-20","volume":"58","author":"RJ Rockett","year":"2020","unstructured":"Rockett RJ, Arnott A, Wang Q, Howard P, Sintchenko V (2020) Genomic surveillance enables suitability assessment of Salmonella gene targets used for culture-independent diagnostic testing. J Clin Microbiol 58:e00038-e120. https:\/\/doi.org\/10.1128\/JCM.00038-20","journal-title":"J Clin Microbiol"},{"key":"4571_CR14","doi-asserted-by":"publisher","unstructured":"Ford L, Glass K, Williamson DA, Sintchenko V, Robson JMB, Lancsar E, Stafford R, Kirk MD (2021) Cost of whole genome sequencing for non-typhoidal Salmonella enterica. PLoS One 16:e0248561. https:\/\/doi.org\/10.1371\/journal.pone.0248561","DOI":"10.1371\/journal.pone.0248561"},{"key":"4571_CR15","doi-asserted-by":"publisher","first-page":"e00644","DOI":"10.1128\/AEM.00644-17","volume":"83","author":"KN Kasturi","year":"2017","unstructured":"Kasturi KN, Drgon T (2017) Real-time PCR method for detection of Salmonella spp. in environmental samples. Appl Environ Microbiol 83:e00644-e717. https:\/\/doi.org\/10.1128\/AEM.00644-17","journal-title":"Appl Environ Microbiol"},{"key":"4571_CR16","doi-asserted-by":"publisher","first-page":"e0239457","DOI":"10.1371\/journal.pone.0239457","volume":"15","author":"PDM Santos","year":"2020","unstructured":"Santos PDM, Widmer KW, Rivera WL (2020) PCR-based detection and serovar identification of\u00a0Salmonella\u00a0in retail meat collected from wet markets in Metro Manila, Philippines. PLoS One 15:e0239457. https:\/\/doi.org\/10.1371\/journal.pone.0239457","journal-title":"PLoS One"},{"key":"4571_CR17","doi-asserted-by":"publisher","first-page":"e63","DOI":"10.1093\/nar\/28.12.e63","volume":"28","author":"T Notomi","year":"2000","unstructured":"Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, Hase T (2000) Loop-mediated isothermal amplification of DNA. Nucleid Acids Res 28:e63. https:\/\/doi.org\/10.1093\/nar\/28.12.e63","journal-title":"Nucleid Acids Res"},{"key":"4571_CR18","doi-asserted-by":"publisher","first-page":"420","DOI":"10.1111\/lam.13409","volume":"72","author":"A Kreitlow","year":"2021","unstructured":"Kreitlow A, Becker A, Schotte U, Malorny B, Pl\u00f6tz M, Abdulmawjood A (2021) Evaluation of different target genes for the detection of Salmonella sp. by loop-mediated isothermal amplification. Lett Appl Microbiol 72:420\u2013426. https:\/\/doi.org\/10.1111\/lam.13409","journal-title":"Lett Appl Microbiol"},{"key":"4571_CR19","doi-asserted-by":"publisher","first-page":"5","DOI":"10.3389\/fsufs.2018.00005","volume":"2","author":"S Azinheiro","year":"2018","unstructured":"Azinheiro S, Carvalho J, Prado M, Garrido-Maestu A (2018) Evaluation of different genetic targets for Salmonella enterica serovar Enteritidis and Typhimurium, using loop-mediated isothermal amplification for detection in food samples. Front Sustain Food Syst 2:5. https:\/\/doi.org\/10.3389\/fsufs.2018.00005","journal-title":"Front Sustain Food Syst"},{"key":"4571_CR20","doi-asserted-by":"publisher","first-page":"560791","DOI":"10.3389\/fmicb.2020.560791","volume":"11","author":"H Shen","year":"2020","unstructured":"Shen H, Wen J, Liao X, Lin Q, Zhang J, Chen K, Wang S, Zhang J (2020) A sensitive, highly specific novel isothermal amplification method based on single-nucleotide polymorphism for the rapid detection of\u00a0Salmonella\u00a0Pullorum. Front Microbiol 11:560791. https:\/\/doi.org\/10.3389\/fmicb.2020.560791","journal-title":"Front Microbiol"},{"key":"4571_CR21","doi-asserted-by":"publisher","first-page":"493","DOI":"10.1007\/s10344-009-0339-3","volume":"56","author":"U Methner","year":"2010","unstructured":"Methner U, Heller M, Bocklisch H (2010) Salmonella enterica subspecies enterica serovar Choleraesuis in a wild boar population in Germany. Eur J Wildl Res 56:493\u2013502. https:\/\/doi.org\/10.1007\/s10344-009-0339-3","journal-title":"Eur J Wildl Res"},{"key":"4571_CR22","unstructured":"G\u00e4rtner A (1888) Ueber die Fleischvergiftung in Frankenhausen a. k. und den Erreger derselben. Cor.-Bl. d. allg. \u00e4rztl. Ver. v. Th\u00fcringen, Weimar, xvii, 573\u2013600"},{"key":"4571_CR23","doi-asserted-by":"publisher","unstructured":"European Centre for Disease Prevention and Control, European Food Safety Authority (2022) Multi-country outbreak of Salmonella Enteritidis sequence type (ST)11 infections linked to eggs and egg products \u2013 8 February 2022. https:\/\/doi.org\/10.2903\/sp.efsa.2022.EN-7180","DOI":"10.2903\/sp.efsa.2022.EN-7180"},{"key":"4571_CR24","doi-asserted-by":"publisher","first-page":"1513","DOI":"10.1017\/S095026881700036X","volume":"145","author":"S Bonardi","year":"2017","unstructured":"Bonardi S (2017) Salmonella in the pork production chain and its impact on human health in the European Union. Epidemiol Infect 145:1513\u20131526. https:\/\/doi.org\/10.1017\/S095026881700036X","journal-title":"Epidemiol Infect"},{"key":"4571_CR25","doi-asserted-by":"publisher","first-page":"115105","DOI":"10.1016\/j.diagmicrobio.2020.115105","volume":"98","author":"M Payen","year":"2020","unstructured":"Payen M, Pardos de la G\u00e1ndara M, Cointe A, Massiot A, Bidet P, Weill FX, Bonacorsi S (2020) Diagnostic challenge of gastrointestinal infection due to lactose-fermenting Salmonella enterica subsp. enterica serovar 4,5:I:-. Diagn Microbiol Infect Dis 98:115105. https:\/\/doi.org\/10.1016\/j.diagmicrobio.2020.115105","journal-title":"Diagn Microbiol Infect Dis"},{"key":"4571_CR26","doi-asserted-by":"publisher","unstructured":"European Centre for Disease Prevention and Control, European Food Safety Authority (2022) Multi-country outbreak of monophasic Salmonella Typhimurium sequence type (ST) 34 infections linked to chocolate products \u2013 12 April 2022. https:\/\/doi.org\/10.2903\/sp.efsa.2022.EN-7318","DOI":"10.2903\/sp.efsa.2022.EN-7318"},{"key":"4571_CR27","doi-asserted-by":"publisher","first-page":"e000365","DOI":"10.1099\/mgen.0.000365","volume":"6","author":"P Alba","year":"2020","unstructured":"Alba P, Leekitcharoenphon P, Carfora V, Amoruso R, Cordaro G, Di Matteo P, Ianzano A, Iurescia M, Diaconu EL, Study Group EN, Pedersen SK, Guerra B, Hendriksen RS, Franco A, Battisti A (2020) Molecular epidemiology of\u00a0Salmonella\u00a0Infantis in Europe: insights into the success of the bacterial host and its parasitic pESI-like megaplasmid. Microb Genom 6:e000365. https:\/\/doi.org\/10.1099\/mgen.0.000365","journal-title":"Microb Genom"},{"key":"4571_CR28","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1017\/S095026881300037X","volume":"142","author":"T Miller","year":"2014","unstructured":"Miller T, Braun PG, Fehlhaber K, Prager R, Pfeifer Y, Rabsch W (2014) Typing of Salmonella enterica serovar Infantis isolates from 51 outbreaks in Germany between 1974 and 2009 by a novel phage-typing scheme. Epidemiol Infect 142:75\u201383. https:\/\/doi.org\/10.1017\/S095026881300037X","journal-title":"Epidemiol Infect"},{"key":"4571_CR29","doi-asserted-by":"publisher","first-page":"1741","DOI":"10.3389\/fmicb.2020.01741","volume":"11","author":"S Garc\u00eda-Soto","year":"2020","unstructured":"Garc\u00eda-Soto S, Abdel-Glil MY, Tomaso H, Linde J, Methner U (2020) Emergence of multidrug-resistant Salmonella enterica subspecies enterica serovar Infantis of multilocus sequence type 2283 in German broiler farms. Front Microbiol 11:1741. https:\/\/doi.org\/10.3389\/fmicb.2020.01741","journal-title":"Front Microbiol"},{"key":"4571_CR30","doi-asserted-by":"publisher","first-page":"1429","DOI":"10.1017\/S0950268815002629","volume":"144","author":"S Schroeder","year":"2016","unstructured":"Schroeder S, Harries M, Prager R, H\u00f6fig A, Ahrens B, Hoffmann L, Rabsch W, Mertens E, Rimek D (2016) A prolonged outbreak of Salmonella Infantis associated with pork products in central Germany, April-October 2013. Epidemiol Infect 144:1429\u20131439. https:\/\/doi.org\/10.1017\/S0950268815002629","journal-title":"Epidemiol Infect"},{"key":"4571_CR31","doi-asserted-by":"publisher","first-page":"891","DOI":"10.3389\/fmicb.2018.00891","volume":"9","author":"Y S\u00e9vellec","year":"2018","unstructured":"S\u00e9vellec Y, Vignaud ML, Granier SA, Lailler R, Feurer C, Le Hello S, Mistou MY, Cadel-Six S (2018) Polyphyletic nature of Salmonella enterica serotype Derby and lineage-specific host-association revealed by genome-wide analysis. Front Microbiol 9:891. https:\/\/doi.org\/10.3389\/fmicb.2018.00891","journal-title":"Front Microbiol"},{"key":"4571_CR32","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1016\/j.ijfoodmicro.2011.08.020","volume":"151","author":"E Hauser","year":"2011","unstructured":"Hauser E, Hebner F, Tietze E, Helmuth R, Junker E, Prager R, Schroeter A, Rabsch W, Fruth A, Malorny B (2011) Diversity of Salmonella enterica serovar Derby isolated from pig, pork and humans in Germany. Int J Food Microbiol 151:141\u2013149. https:\/\/doi.org\/10.1016\/j.ijfoodmicro.2011.08.020","journal-title":"Int J Food Microbiol"},{"key":"4571_CR33","doi-asserted-by":"publisher","first-page":"46","DOI":"10.1016\/j.fm.2017.08.017","volume":"71","author":"S Simon","year":"2018","unstructured":"Simon S, Trost E, Bender J, Fuchs S, Malorny B, Rabsch W, Prager R, Tietze E, Flieger A (2018) Evaluation of WGS based approaches for investigating a food-borne outbreak caused by Salmonella enterica serovar Derby in Germany. Food Microbiol 71:46\u201354. https:\/\/doi.org\/10.1016\/j.fm.2017.08.017","journal-title":"Food Microbiol"},{"key":"4571_CR34","doi-asserted-by":"publisher","first-page":"46","DOI":"10.3390\/pathogens8020046","volume":"8","author":"Y S\u00e9vellec","year":"2019","unstructured":"S\u00e9vellec Y, Felten A, Radomski N, Granier SA, Le Hello S, Petrovska L, Mistou MY (2019) Cadel-Six S (2019) Genetic diversity of Salmonella Derby from the poultry sector in Europe. Pathogens 8:46. https:\/\/doi.org\/10.3390\/pathogens8020046","journal-title":"Pathogens"},{"key":"4571_CR35","doi-asserted-by":"publisher","unstructured":"European Centre for Disease Prevention and Control, European Food Safety Authority (2021) Multi-country outbreak of Salmonella Braenderup ST22, presumed to be linked to imported melons - 20 July 2021. https:\/\/doi.org\/10.2903\/sp.efsa.2021.EN-6807","DOI":"10.2903\/sp.efsa.2021.EN-6807"}],"container-title":["European Journal of Clinical Microbiology &amp; Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10096-023-04571-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10096-023-04571-3\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10096-023-04571-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,3,9]],"date-time":"2023-03-09T11:10:08Z","timestamp":1678360208000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10096-023-04571-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,22]]},"references-count":35,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2023,4]]}},"alternative-id":["4571"],"URL":"https:\/\/doi.org\/10.1007\/s10096-023-04571-3","relation":{},"ISSN":["0934-9723","1435-4373"],"issn-type":[{"value":"0934-9723","type":"print"},{"value":"1435-4373","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,22]]},"assertion":[{"value":"29 June 2022","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"13 February 2023","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 February 2023","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Because in this work, a method for subtyping bacterial isolates was developed, and the paper contains no patient data, and research ethics committee approval was not required.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"All the authors have given their consent for publication of the study.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"L.W. is managing director of Amplex Diagnostics, whose lyophilization service was used for this project. The other authors declare that there are no conflicts of interest.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}