{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T19:55:40Z","timestamp":1790711740344,"version":"4.1.0"},"reference-count":26,"publisher":"Springer Science and Business Media LLC","issue":"6","license":[{"start":{"date-parts":[[2024,4,19]],"date-time":"2024-04-19T00:00:00Z","timestamp":1713484800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,4,19]],"date-time":"2024-04-19T00:00:00Z","timestamp":1713484800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Beijing Natural Science Foundation","award":["7232150"],"award-info":[{"award-number":["7232150"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Eur J Clin Microbiol Infect Dis"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Purpose<\/jats:title>\n                <jats:p>This study was to clarify the molecular epidemiology and clinical infection characteristics of <jats:italic>Ralstonia pickettii<\/jats:italic> and establish sequence typing system.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>48 nonrepetitive <jats:italic>Ralstonia pickettii<\/jats:italic> strains were collected from January 2008 to December 2013 at the Chinese People\u2019s Liberation Army General Hospital (PLAGH) and were identified through a specific PCR experiment, 16\u00a0S rDNA experiment and VITEK 2 system to compare the identification accuracy. The sequence types of the strains were analyzed by multilocus sequence typing (MLST) method. The antibiotic sensitivity of these strains was determined with disc diffusion tests and broth microdilution method. The clinical data of <jats:italic>Ralstonia pickettii<\/jats:italic> infected patients were collected.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>All of the 48 strains were identified as <jats:italic>Ralstonia pickettii<\/jats:italic> by VITEK 2 system. 30 and 34 strains were identified as <jats:italic>Ralstonia pickettii<\/jats:italic> by PCR and 16\u00a0S rDNA experiment respectively. ST9 was the most sequence types (STs) in these 18 STs of 42 strains. 42 strains were divided into 2 groups (A and B) and 18 genotypes. <jats:italic>Ralstonia pickettii<\/jats:italic> was sensitive to some cephalosporins, \u03b2-lactam\/\u03b2-lactamase inhibitor, levofloxacin and trimethoprim\/sulfamethoxazole. Cough, sputum, shortness of breath and pulmonary rales were the common clinical symptoms of most <jats:italic>Ralstonia pickettii<\/jats:italic> infected patients.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>We established a sequence typing system with a relatively fine resolution and the PCR assay is a faster and more sensitive method for clinical identification of <jats:italic>Ralstonia pickettii<\/jats:italic>. ST9 is the most common sequence types of <jats:italic>Ralstonia pickettii<\/jats:italic>. The most common clinical characteristics of <jats:italic>Ralstonia pickettii<\/jats:italic> infected patients were cough, sputum, shortness of breath and pulmonary rales.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1007\/s10096-024-04823-w","type":"journal-article","created":{"date-parts":[[2024,4,19]],"date-time":"2024-04-19T14:01:45Z","timestamp":1713535305000},"page":"1161-1170","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Molecular epidemiological and clinical infection characteristics analysis of Ralstonia"],"prefix":"10.1007","volume":"43","author":[{"given":"Zhaojun","family":"Sheng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxin","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guojing","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ru","family":"Fan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pingjun","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9687-9683","authenticated-orcid":false,"given":"Xiangqun","family":"Fang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,4,19]]},"reference":[{"issue":"11","key":"4823_CR1","doi-asserted-by":"publisher","first-page":"897","DOI":"10.1111\/j.1348-0421.1995.tb03275.x","volume":"39","author":"E Yabuuchi","year":"1995","unstructured":"Yabuuchi E, Kosako Y, Yano I et al (1995) Transfer of two Burkholderia and an Alcaligenes species to Ralstonia gen. 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