{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T06:36:41Z","timestamp":1774766201670,"version":"3.50.1"},"reference-count":8,"publisher":"Springer Science and Business Media LLC","issue":"7","license":[{"start":{"date-parts":[[2024,5,27]],"date-time":"2024-05-27T00:00:00Z","timestamp":1716768000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,5,27]],"date-time":"2024-05-27T00:00:00Z","timestamp":1716768000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100011091","name":"FOD Volksgezondheid, Veiligheid van de Voedselketen en Leefmilieu","doi-asserted-by":"publisher","award":["FOD Volksgezondheid, Veiligheid van de Voedselketen en Leefmilieu"],"award-info":[{"award-number":["FOD Volksgezondheid, Veiligheid van de Voedselketen en Leefmilieu"]}],"id":[{"id":"10.13039\/501100011091","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Eur J Clin Microbiol Infect Dis"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Purpose<\/jats:title>\n                <jats:p>To assess performance of Etest\u00ae, Vitek\u00ae2 and BD Phoenix\u2122 to determine the susceptibility of <jats:italic>Streptococcus pneumoniae<\/jats:italic> strains to penicillin, ampicillin and cefotaxime.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Sixty unique <jats:italic>S. pneumoniae<\/jats:italic> challenge strains were selected to cover a wide range of penicillin, ampicillin and cefotaxime minimal inhibitory concentrations (MICs). Strains were analyzed in four different Belgian laboratories. Etest\u00ae benzylpenicillin (BEN), ampicillin\/amoxicillin (AMP) and cefotaxime (CTA) (bioM\u00e9rieux), Vitek\u00ae2 AST-ST03 (bioM\u00e9rieux) and BD Phoenix\u2122 SMIC\/ID-11 testing were each performed in two different labs. Results were compared to Sensititre\u00ae broth microdilution (BMD) (Thermo Fisher Scientific) results. MIC results were interpreted using EUCAST non-meningitis breakpoints (v 13.0).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Essential agreement (EA) was \u2265\u200990% for all methods compared to BMD, except for Etest\u00ae BEN on Oxoid plate (58.3%), Etest\u00ae AMP (both on Oxoid (65.8%) and BD BBL plate (84.2%)). Categorical agreement (CA) for penicillin was only\u2009\u2265\u200990% for Vitek\u00ae2, for other methods CA ranged between 74 and 84%. CA for AMP was for all methods\u2009&lt;\u200990% (range 75.8\u201388.3%) and CA for CTA was between 87 and 90% for all methods except for Etest on Oxoid plate (79.2%).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Our study indicates that Vitek\u00ae2 and BD Phoenix\u2122 are reliable for providing accurate pneumococcal susceptibility results for BEN, AMP and CTA. Using Etest BEN or AMP on Oxoid plate carries a risk of underestimating the MIC and should be interpreted with caution, especially when the obtained MIC is 1 or 2 doubling dilutions below the S or R clinical breakpoint.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1007\/s10096-024-04847-2","type":"journal-article","created":{"date-parts":[[2024,5,27]],"date-time":"2024-05-27T12:02:42Z","timestamp":1716811362000},"page":"1375-1381","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Multicenter comparison of Etest, Vitek2 and BD Phoenix to broth microdilution for beta-lactam susceptibility testing of Streptococcus pneumonia"],"prefix":"10.1007","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-1723-1061","authenticated-orcid":false,"given":"Steven","family":"Martens","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lize","family":"Cuypers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Florian","family":"B\u00e9lik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pieter-Jan","family":"Briers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pieter-Jan","family":"Ceyssens","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olivier","family":"Denis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Te-Din","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koen","family":"Magerman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Strypens","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne-Marie","family":"Van den Abeele","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefanie","family":"Desmet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"on behalf of the Belgian National Antibiogram Committee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,5,27]]},"reference":[{"key":"4847_CR1","volume-title":"Antimicrobial resistance in the EU\/EEA (EARS-Net) - Annual Epidemiological Report 2021","author":"European Centre for Disease Prevention and Control","year":"2022","unstructured":"European Centre for Disease Prevention and Control (2022) Antimicrobial resistance in the EU\/EEA (EARS-Net) - Annual Epidemiological Report 2021. ECDC, Stockholm"},{"key":"4847_CR2","unstructured":"EUCAST (2019) Warning against the use of gradient tests for benzylpenicillin MIC in Streptococcus pneumoniae. https:\/\/www.eucast.org\/fileadmin\/src\/media\/PDFs\/EUCAST_files\/Warnings\/Warnings_docs\/Warning_-_gradient_for_benzyl_and_pnc_21nov2019b.pdf. Accessed 14 September 2023"},{"key":"4847_CR3","unstructured":"CLSI (2015) Verification of Commercial Microbial Identification and Antimicrobial Susceptibility Testing Systems. 1st ed. CLSI guideline M52. Wayne, PA: Clinical and Laboratory Standards Institute"},{"key":"4847_CR4","unstructured":"International Standard Organisation ISO 20776-2:2021 (2021) Clinical laboratory testing and in vitro diagnostic test systems_Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices. Part 2_Evaluation of performance of antimicrobial susceptibility test devices against reference broth micro-dilution AFNOR. Gen\u00e8ve"},{"key":"4847_CR5","unstructured":"The European Committee on Antimicrobial Susceptibility Testing (2023) Breakpoint tables for interpretation of MICs and zone diameters. Version 13.0"},{"issue":"4","key":"4847_CR6","doi-asserted-by":"publisher","first-page":"e01934","DOI":"10.1128\/JCM.01934-17","volume":"56","author":"RM Humphries","year":"2018","unstructured":"Humphries RM, Ambler J, Mitchell SL, Castanheira M, Dingle T, Hindler JA, Koeth L, Sei K, CLSI Methods Development and Standardization Working Group of the Subcommittee on Antimicrobial Susceptibility Testing (2018) CLSI Methods Development and Standardization Working Group Best Practices for Evaluation of Antimicrobial susceptibility tests. J Clin Microbiol 56(4):e01934\u2013e01917. https:\/\/doi.org\/10.1128\/JCM.01934-17","journal-title":"J Clin Microbiol"},{"key":"4847_CR7","doi-asserted-by":"crossref","unstructured":"Marthe K, Charles BM, Berenger, LeeAnn Turnbull R, Rennie (2016) Variability of \u03b2-lactam susceptibility testing for Streptococcus pneumoniae using 4 commercial test methods and broth microdilution, Diagnostic Microbiology and Infectious Disease. 84(3):240\u2013245Jeff Fuller","DOI":"10.1016\/j.diagmicrobio.2015.11.014"},{"issue":"11","key":"4847_CR8","doi-asserted-by":"publisher","first-page":"1499e1","DOI":"10.1016\/j.cmi.2022.05.030","volume":"28","author":"J \u00c5hman","year":"2022","unstructured":"\u00c5hman J, Matuschek E, Kahlmeter G (2022) Evaluation of ten brands of pre-poured Mueller-Hinton agar plates for EUCAST disc diffusion testing. Clin Microbiol Infect 28(11):1499e1\u20131499e5. https:\/\/doi.org\/10.1016\/j.cmi.2022.05.030","journal-title":"Clin Microbiol Infect"}],"container-title":["European Journal of Clinical Microbiology &amp; Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10096-024-04847-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s10096-024-04847-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s10096-024-04847-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,19]],"date-time":"2024-07-19T14:06:02Z","timestamp":1721397962000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s10096-024-04847-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,27]]},"references-count":8,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2024,7]]}},"alternative-id":["4847"],"URL":"https:\/\/doi.org\/10.1007\/s10096-024-04847-2","relation":{},"ISSN":["0934-9723","1435-4373"],"issn-type":[{"value":"0934-9723","type":"print"},{"value":"1435-4373","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,27]]},"assertion":[{"value":"27 December 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 May 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 May 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Not required.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical approval"}},{"value":"The authors have no relevant financial or non-financial interests to disclose.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}